FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567359
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2075768.085195720582902TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA277001.078929748430196No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC253660.9880192057285327No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC117770.45872042047878775No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT101330.3946857451567934No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT87280.33996024708659756No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC53000.20643782190180648No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT51120.1991151218041575No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT49460.19264933342006318TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT36540.14232524551494355No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31860.12409639633568972No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG29480.11482616961632558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG286600.080.737913
GATCGGA288550.080.369271
CGGAAGA285500.080.313884
GAAGAGC285850.080.192696
ATCGGAA290550.079.460472
GGAAGAG302600.075.9577565
GAGCACA367250.062.3923269
AGAGCAC368900.062.164758
AAGAGCA369400.061.9777687
GCGCACA2550.048.4204529
GTACGGG2050.046.4005971
ATCGGAC1250.045.5950472
GCCGTCT247650.045.5187650-51
ATGCCGT247000.045.42566348-49
TCGTATG252250.045.35603744-45
TCTCGTA246550.045.3346542-43
AATCTCG246950.045.13142840-41
CTGCTTG252200.045.10427558-59
GTATGCC252750.045.06532746-47
TATGCCG249350.044.85466848-49
Produced by FastQC (version 0.11.2)