FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139482
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT304131.4215123099890534TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC24570.11484088204528012No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG22000.10282862861197242No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC21950.10259492718330886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTC200.002076671771.229676
TCGGAAG48650.067.458383
CGGAAGA49400.066.241934
GATCGGA51300.063.8900761
ATCGGAA51850.063.2034872
GAAGAGC56950.057.367296
GGAAGAG67250.049.075215
GCCGTCT36050.044.21349750-51
TGCCGTC35750.043.9200750-51
ATGCCGT36250.043.7678648-49
TATGCCG36150.043.56042548-49
CGTCTTC37200.042.7843352-53
TTGTGTT236300.042.6980971
CGTATGC37150.042.63662346-47
CCGTCTT36850.042.54605552-53
AATCTCG37300.042.33585440-41
TCGTATG38400.042.1719144-45
CACTCCG38450.042.0528630-31
GTATGCC38250.041.96923446-47
TCTCGTA37700.041.8832442-43
Produced by FastQC (version 0.11.2)