##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l03n01_30230-1.34100000035714.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2185036 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.686433999256764 33.0 33.0 33.0 33.0 33.0 2 32.66122892254407 33.0 33.0 33.0 33.0 33.0 3 32.72512581028413 33.0 33.0 33.0 33.0 33.0 4 36.68204368257548 37.0 37.0 37.0 37.0 37.0 5 36.686254139519896 37.0 37.0 37.0 37.0 37.0 6 36.57749483303708 37.0 37.0 37.0 37.0 37.0 7 36.51590408579081 37.0 37.0 37.0 37.0 37.0 8 36.44397071718727 37.0 37.0 37.0 37.0 37.0 9 36.44955826814753 37.0 37.0 37.0 37.0 37.0 10-11 36.312868987055595 37.0 37.0 37.0 37.0 37.0 12-13 36.341811530794004 37.0 37.0 37.0 37.0 37.0 14-15 38.21281205435517 40.0 37.0 40.0 37.0 40.0 16-17 38.1288479915205 40.0 37.0 40.0 37.0 40.0 18-19 38.09202068066613 40.0 37.0 40.0 37.0 40.0 20-21 38.08081125436835 40.0 37.0 40.0 35.0 40.0 22-23 37.97480270347948 40.0 37.0 40.0 33.0 40.0 24-25 37.98262339842456 40.0 37.0 40.0 33.0 40.0 26-27 37.87774984027723 40.0 37.0 40.0 33.0 40.0 28-29 37.74574423487759 40.0 37.0 40.0 33.0 40.0 30-31 37.64783944063164 40.0 37.0 40.0 33.0 40.0 32-33 37.585923527118084 40.0 37.0 40.0 33.0 40.0 34-35 37.47115997173502 40.0 37.0 40.0 33.0 40.0 36-37 37.37444142796732 40.0 37.0 40.0 33.0 40.0 38-39 37.23618146337177 40.0 37.0 40.0 33.0 40.0 40-41 37.08190505785717 38.5 37.0 40.0 33.0 40.0 42-43 36.94631141088751 37.0 37.0 40.0 33.0 40.0 44-45 36.80454944449428 37.0 37.0 40.0 33.0 40.0 46-47 36.76577250901129 37.0 37.0 40.0 33.0 40.0 48-49 36.59020377696294 37.0 37.0 40.0 33.0 40.0 50-51 36.463125321505004 37.0 37.0 40.0 33.0 40.0 52-53 36.29921841104678 37.0 37.0 40.0 33.0 40.0 54-55 35.92493716350669 37.0 37.0 40.0 30.0 40.0 56-57 35.75985864763784 37.0 35.0 40.0 30.0 40.0 58-59 35.614566991115936 37.0 33.0 40.0 27.0 40.0 60-61 35.37298424373786 37.0 33.0 40.0 27.0 40.0 62-63 35.1187554804589 37.0 33.0 37.0 27.0 40.0 64-65 34.87053005076346 37.0 33.0 37.0 27.0 40.0 66-67 34.301991591900546 37.0 33.0 37.0 27.0 40.0 68-69 34.03054343269402 37.0 33.0 37.0 27.0 38.5 70-71 33.74340720244426 37.0 33.0 37.0 27.0 37.0 72-73 33.25408254143181 37.0 33.0 37.0 27.0 37.0 74-75 32.74326715898502 37.0 33.0 37.0 24.5 37.0 76-77 29.974907049586367 33.0 30.0 33.0 22.0 37.0 78-79 32.4315265286247 33.0 33.0 37.0 27.0 37.0 80-81 32.918174803527265 37.0 33.0 37.0 27.0 37.0 82-83 32.69957176906925 37.0 33.0 37.0 27.0 37.0 84-85 32.63694030670433 37.0 33.0 37.0 27.0 37.0 86-87 32.55822100871565 37.0 33.0 37.0 22.0 37.0 88-89 32.44642079123639 33.0 33.0 37.0 22.0 37.0 90-91 32.390297230800776 33.0 33.0 37.0 22.0 37.0 92-93 32.2041545310924 33.0 33.0 37.0 22.0 37.0 94-95 32.01056344151767 33.0 33.0 37.0 22.0 37.0 96-97 31.842974440695713 33.0 33.0 37.0 22.0 37.0 98-99 31.698414808726262 33.0 33.0 37.0 18.5 37.0 100-101 29.97622419035659 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15544448688221024 2306 2 0.15167479931248096 2306 3 0.13776951764014456 2306 4 0.14376926337738638 2306 5 0.15341309226270283 2306 6 0.15992803396832755 2306 7 0.20080621311819158 2306 8 0.12001325367821636 2306 9 0.23134831685254653 2306 10-11 0.27973688998666546 2306 12-13 0.23932507381817203 2306 14-15 0.47571035734036826 2306 16-17 0.5335541520477207 2306 18-19 0.6146018085717486 2306 20-21 0.5944243967670744 2306 22-23 0.5658632996023414 2306 24-25 0.49512260533725083 2306 26-27 0.44414512552373964 2306 28-29 0.469281454286282 2306 30-31 0.6007250173692071 2306 32-33 0.5541293957375473 2306 34-35 0.5649761733635046 2306 36-37 0.5729821067357932 2306 38-39 0.6384009247829496 2306 40-41 0.6663617160046726 2306 42-43 0.6830321489343518 2306 44-45 0.7435086102120394 2306 46-47 0.641606774019877 2306 48-49 0.6443991246633018 2306 50-51 0.7098226749945624 2306 52-53 0.6073894778287752 2306 54-55 0.6791837118232991 2306 56-57 0.7656113913831675 2306 58-59 0.6819016521198833 2306 60-61 0.7638813039187724 2306 62-63 0.6480893914487993 2306 64-65 0.5375241887443778 2306 66-67 0.6718266161381123 2306 68-69 0.7135048992819222 2306 70-71 0.7014527310439789 2306 72-73 0.625829372340533 2306 74-75 0.5522650192268586 2306 76-77 0.20538804128488053 2306 78-79 0.5431243718179815 2306 80-81 0.7026888540212965 2306 82-83 0.538838176109472 2306 84-85 0.5229267868755016 2306 86-87 0.5833589476668521 2306 88-89 0.6220542650737926 2306 90-91 0.6916859114806755 2306 92-93 0.5328167728551492 2306 94-95 0.5145777635567441 2306 96-97 0.8224491766259945 2306 98-99 0.6434222330591446 2306 100-101 0.7246410542597097 2307 1 0.16638735182247189 2307 2 0.21362517390006275 2307 3 0.157258258675526 2307 4 0.14236858217139314 2307 5 0.16095921924150502 2307 6 0.08857451132392669 2307 7 0.1214316396462678 2307 8 0.1899041485276669 2307 9 0.22261384780880178 2307 10-11 0.13086787983464632 2307 12-13 0.14927400573852623 2307 14-15 0.18877306896970225 2307 16-17 0.2642053930960131 2307 18-19 0.3786549945364399 2307 20-21 0.3927581367682933 2307 22-23 0.26027802306759895 2307 24-25 0.2790544517000768 2307 26-27 0.35479284938237043 2307 28-29 0.3102174121818706 2307 30-31 0.3901905869388358 2307 32-33 0.33892918264128014 2307 34-35 0.39283572255064314 2307 36-37 0.2904421658121876 2307 38-39 0.4068550717457242 2307 40-41 0.3984926201598853 2307 42-43 0.37468061305471423 2307 44-45 0.33480136325267296 2307 46-47 0.41644434490140014 2307 48-49 0.40406098576597316 2307 50-51 0.35617028690193564 2307 52-53 0.25074076764279596 2307 54-55 0.40063708892763117 2307 56-57 0.4694493955466683 2307 58-59 0.25929152571099223 2307 60-61 0.18976857695765403 2307 62-63 0.3686008362531794 2307 64-65 0.40868010176998837 2307 66-67 0.46408133087579273 2307 68-69 0.31733564192860086 2307 70-71 0.25569350848743255 2307 72-73 0.2864673740261736 2307 74-75 0.36920510551846064 2307 76-77 -0.07369252679287186 2307 78-79 0.3103225516895378 2307 80-81 0.4347232247420365 2307 82-83 0.47434334538024103 2307 84-85 0.5028454234723725 2307 86-87 0.49500831345422824 2307 88-89 0.40173657543377317 2307 90-91 0.4194406332733749 2307 92-93 0.3823424856797075 2307 94-95 0.23565963320115202 2307 96-97 0.4276045438333256 2307 98-99 0.24564339683593417 2307 100-101 0.2728683665796119 2201 1 0.1638198046267405 2201 2 0.1768471991487246 2201 3 0.11727760830656564 2201 4 0.1243887548932463 2201 5 0.11592139459292383 2201 6 0.1282663802171342 2201 7 0.12206263353160551 2201 8 0.19170458017327974 2201 9 0.12756371496296026 2201 10-11 0.20782543977111345 2201 12-13 0.2104190306506979 2201 14-15 0.36917180367429836 2201 16-17 0.2291386394132573 2201 18-19 0.26455085001339285 2201 20-21 0.2824140351935469 2201 22-23 0.28249241087313237 2201 24-25 0.24839543201777303 2201 26-27 0.22908624118441168 2201 28-29 0.3516605753440487 2201 30-31 0.36186042447699407 2201 32-33 0.4368243536934173 2201 34-35 0.4666394262048712 2201 36-37 0.4112059355681197 2201 38-39 0.4857606344784102 2201 40-41 0.35271328228364496 2201 42-43 0.3639638943246908 2201 44-45 0.2184599573957513 2201 46-47 0.4264284555594031 2201 48-49 0.4294887035717636 2201 50-51 0.23684597194692714 2201 52-53 0.2732234232619746 2201 54-55 0.6009790787083347 2201 56-57 0.38934115974068817 2201 58-59 0.2996326758627248 2201 60-61 0.37044241736327166 2201 62-63 0.43696619316227725 2201 64-65 0.31393687875245035 2201 66-67 0.4121575946286953 2201 68-69 0.4993837386742186 2201 70-71 0.2773751771609412 2201 72-73 0.37086439639655566 2201 74-75 0.4756500210880432 2201 76-77 0.16952794641438018 2201 78-79 0.35270823016380604 2201 80-81 0.3644885802517521 2201 82-83 0.34423520498665283 2201 84-85 0.5274276285542925 2201 86-87 0.49022703997881223 2201 88-89 0.517033851791922 2201 90-91 0.6150576428839472 2201 92-93 0.5611853624775165 2201 94-95 0.5350633694228151 2201 96-97 0.44891299107314353 2201 98-99 0.3561889775570428 2201 100-101 0.2122559017788319 1101 1 0.01140292790660169 1101 2 0.13183340835901447 1101 3 0.09772081370768859 1101 4 0.07110049027634346 1101 5 0.10386744276716797 1101 6 0.08184946193164677 1101 7 0.13817989532503105 1101 8 0.13009136951711042 1101 9 0.013977783052943948 1101 10-11 0.17131072415234172 1101 12-13 0.10613572121744141 1101 14-15 0.23774988270191244 1101 16-17 0.21787428081115223 1101 18-19 0.3349805169858584 1101 20-21 0.33388084674515284 1101 22-23 0.1670198573651689 1101 24-25 0.2335778296853661 1101 26-27 0.18683045988941416 1101 28-29 0.2980990185435033 1101 30-31 0.3201029820524326 1101 32-33 0.3470094976431355 1101 34-35 0.2854519041232848 1101 36-37 0.33420641950756647 1101 38-39 0.3091353550256528 1101 40-41 0.35880602051990707 1101 42-43 0.47558022238219877 1101 44-45 0.4144676054203629 1101 46-47 0.2870228697700057 1101 48-49 0.3433712339515793 1101 50-51 0.17727183865872576 1101 52-53 0.209419718601616 1101 54-55 0.5032964954811447 1101 56-57 0.4026747305903129 1101 58-59 0.31395565051676755 1101 60-61 0.4570304661372475 1101 62-63 0.3907498276959629 1101 64-65 0.3048593029624911 1101 66-67 0.49226026104597054 1101 68-69 0.38469038705212455 1101 70-71 0.27518601918588104 1101 72-73 0.3122269679854526 1101 74-75 0.2585158926689175 1101 76-77 -0.024030611742968944 1101 78-79 0.6370746109202372 1101 80-81 0.7525732785615276 1101 82-83 0.6960473058479479 1101 84-85 0.6540521657686398 1101 86-87 0.6097186660450333 1101 88-89 0.6411313641056609 1101 90-91 0.46493665802264417 1101 92-93 0.4651578224634463 1101 94-95 0.4851846511794804 1101 96-97 0.4654548132387575 1101 98-99 0.3010446715647994 1101 100-101 0.4920739939885941 2304 1 0.11779494214648167 2304 2 0.18780679648365606 2304 3 0.13730817581378574 2304 4 0.11069691712116025 2304 5 1.5859118715866316E-4 2304 6 0.11528653616361595 2304 7 0.08652819005539669 2304 8 0.19412138753671115 2304 9 0.07957023240028605 2304 10-11 0.14320165611735547 2304 12-13 0.1281539715580564 2304 14-15 0.15526364462341036 2304 16-17 0.1495360397000951 2304 18-19 0.29324192756908474 2304 20-21 0.2905404081216716 2304 22-23 0.18110989494263663 2304 24-25 0.1283127272812905 2304 26-27 0.054646929146208834 2304 28-29 0.15583989337319792 2304 30-31 0.26640305786661145 2304 32-33 0.21314514459078993 2304 34-35 0.2831027633506338 2304 36-37 0.22948272922495505 2304 38-39 0.20613176758663343 2304 40-41 0.42542003845161247 2304 42-43 0.20002521315264232 2304 44-45 0.4716806652366472 2304 46-47 0.3710637105382588 2304 48-49 0.3410576654111708 2304 50-51 0.17390799652802258 2304 52-53 0.38182033610259936 2304 54-55 0.6031238534119652 2304 56-57 0.46003873204067247 2304 58-59 0.32654121615646403 2304 60-61 0.39756852705229306 2304 62-63 0.24176219419707934 2304 64-65 0.010942704052453678 2304 66-67 0.2138616707501768 2304 68-69 0.20694267246503983 2304 70-71 0.10919403461242183 2304 72-73 -0.033257281344305056 2304 74-75 0.22746517957614998 2304 76-77 0.16904118098765863 2304 78-79 0.08099752767846269 2304 80-81 0.32476472034942105 2304 82-83 0.22729661912531185 2304 84-85 0.21184166056053755 2304 86-87 0.367301182636254 2304 88-89 0.2247218521731682 2304 90-91 0.06942480244315874 2304 92-93 0.10128904691736551 2304 94-95 0.23824935693257032 2304 96-97 0.1977292660581682 2304 98-99 0.277328156160241 2304 100-101 0.25977056147420186 2202 1 0.11449348495158063 2202 2 0.15611768641339552 2202 3 0.10108159501753278 2202 4 0.04839207715238558 2202 5 0.08092231744268474 2202 6 0.06303243780295986 2202 7 0.10440159624847922 2202 8 0.011402808301738787 2202 9 0.027584479082563007 2202 10-11 0.16512056712438294 2202 12-13 0.1310366478526177 2202 14-15 0.12570096572671474 2202 16-17 0.09594834779272787 2202 18-19 0.18011009696798652 2202 20-21 0.217774177312414 2202 22-23 0.27485582910864537 2202 24-25 0.2523694537970229 2202 26-27 0.13276379747824052 2202 28-29 0.14212176951162547 2202 30-31 0.17853631964126038 2202 32-33 0.23538932231652865 2202 34-35 0.34200856610889474 2202 36-37 0.19587047968033744 2202 38-39 0.24609078398007256 2202 40-41 0.3932552258052411 2202 42-43 0.18971832400354316 2202 44-45 0.22730777947031555 2202 46-47 0.17770890960149188 2202 48-49 0.2331870239852023 2202 50-51 0.21510709228654434 2202 52-53 0.2912132257721325 2202 54-55 0.35200574906640014 2202 56-57 0.41724582936047483 2202 58-59 0.21444210738727065 2202 60-61 0.23171502718239623 2202 62-63 0.18702387127228093 2202 64-65 0.10454226819505408 2202 66-67 -0.016252335234725024 2202 68-69 0.10935859101825685 2202 70-71 0.20300502212217708 2202 72-73 0.22467922659220108 2202 74-75 0.2624313518373995 2202 76-77 -0.033262520547598484 2202 78-79 0.2583410743867631 2202 80-81 0.3776449571435947 2202 82-83 0.3885435290914856 2202 84-85 0.22286413649080572 2202 86-87 0.4402951001486102 2202 88-89 0.35094973403629837 2202 90-91 0.03795931324201973 2202 92-93 0.27769745180936667 2202 94-95 0.3502915440259926 2202 96-97 0.28555128010968645 2202 98-99 0.3289764452573003 2202 100-101 0.2816772749792733 1102 1 0.10094818417982765 1102 2 0.14849314516017387 1102 3 0.09243336883838538 1102 4 0.07503903469848439 1102 5 0.0715708683314844 1102 6 0.06615446444425288 1102 7 0.08287778294896953 1102 8 0.046984000816806315 1102 9 0.06676626740208036 1102 10-11 0.18019974341906675 1102 12-13 0.17305704981296088 1102 14-15 0.21068144539083278 1102 16-17 0.194189063362181 1102 18-19 0.22308504146529629 1102 20-21 0.2162844302083684 1102 22-23 0.18301113848897899 1102 24-25 0.19156167247010103 1102 26-27 0.11171713745680023 1102 28-29 0.23885338754736551 1102 30-31 0.18918566342571808 1102 32-33 0.2885765740646775 1102 34-35 0.21564554072113395 1102 36-37 0.12153981576237527 1102 38-39 0.13832139768130958 1102 40-41 0.22583601620893745 1102 42-43 0.12654011504155704 1102 44-45 0.03276563895411044 1102 46-47 0.09948833735737139 1102 48-49 0.13541213461302704 1102 50-51 0.03974712709842976 1102 52-53 0.27531024033120133 1102 54-55 0.25512853084641307 1102 56-57 0.3170828137895114 1102 58-59 0.2592260810782392 1102 60-61 0.33124702996131106 1102 62-63 0.09415077610970002 1102 64-65 -0.06755762155469114 1102 66-67 -0.03020257914452884 1102 68-69 0.0485635526572068 1102 70-71 0.06970368779206382 1102 72-73 -0.08060801873726575 1102 74-75 0.034135690486913006 1102 76-77 -0.36049645685919884 1102 78-79 -0.06695168345534341 1102 80-81 0.011751694113677047 1102 82-83 -0.01207313606490601 1102 84-85 0.11213153866258807 1102 86-87 0.22245669417093694 1102 88-89 -0.07160819454268363 1102 90-91 -0.09852658265456427 1102 92-93 -0.09214353485487692 1102 94-95 -0.09349899748398727 1102 96-97 0.24880693798654363 1102 98-99 0.3362857160953503 1102 100-101 0.16947404449583559 2305 1 0.1121335344718517 2305 2 0.19234182091399532 2305 3 0.1062712753464865 2305 4 0.1571763986804413 2305 5 0.08623082431804363 2305 6 0.0678780738289575 2305 7 0.03167836814571956 2305 8 0.0995527618764882 2305 9 -0.04856537500948832 2305 10-11 0.15597644986745252 2305 12-13 0.09928992525457403 2305 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Count 7 3.0 8 3.0 9 7.0 10 8.0 11 14.0 12 26.0 13 77.0 14 243.0 15 721.0 16 1465.0 17 2354.0 18 3062.0 19 3576.0 20 4542.0 21 5802.0 22 7331.0 23 9039.0 24 11780.0 25 14609.0 26 18840.0 27 25691.0 28 37308.0 29 52901.0 30 52387.0 31 52920.0 32 70669.0 33 101226.0 34 158585.0 35 283873.0 36 517654.0 37 637624.0 38 110642.0 39 54.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.512332946467694 21.919880310005883 23.714680175794676 12.85310656773174 2 35.41506867621403 20.8756285937623 31.40351005658488 12.30579267343879 3 41.93106516228213 15.275989695152061 27.76455372127391 15.028391421291895 4 35.905077242485376 14.403599767119124 31.599801904789803 18.09152108560569 5 35.58403614402692 13.289254730814504 34.933017121914695 16.193692003243882 6 29.747015609811463 13.926589767857372 39.610148299616114 16.71624632271505 7 36.92314451569676 17.254955982418597 27.59075823007035 18.231141271814284 8 33.70992514539806 27.52911164850373 21.06642636551526 17.69453684058295 9 34.6355391856244 25.498435723713474 21.329122266177766 18.536902824484354 10-11 30.152478036975133 26.873401628165393 26.493430771849987 16.480689563009488 12-13 28.34336101175473 25.176570357781376 26.29973288763501 20.18033574282889 14-15 27.014886711248693 25.687883403294045 26.047374047841775 21.24985583761549 16-17 27.116235155850983 26.318170501538646 26.68313016353049 19.882464179079886 18-19 24.677282204961383 26.401601621208986 27.79244827087517 21.128667902954458 20-21 24.993238094584296 27.251050898498193 28.350781566427997 19.404929440489514 22-23 24.340553890567858 28.191660131682134 27.280484587314298 20.18730139043571 24-25 25.200477246141485 24.13651308262198 29.357159332843946 21.30585033839259 26-27 22.511803009195273 24.23218656351657 29.48747755185727 23.768532875430886 28-29 23.078178116973817 27.412385882887058 27.0367856639433 22.472650336195834 30-31 20.27437991868326 27.09410737397462 28.96165097508691 23.669861732255214 32-33 20.975672712028544 26.511439628454635 28.228962818003918 24.283924841512906 34-35 21.901446932682116 26.712740659650457 28.831081043973644 22.55473136369378 36-37 20.341360563582683 29.39809274144276 29.53230197787573 20.72824471709883 38-39 20.230998663152388 27.560708488044117 27.186404886793586 25.021887962009913 40-41 23.184292915989957 26.680609336951193 25.38299816536184 24.752099581697006 42-43 23.32332941643564 28.381505072706698 26.86599747594655 21.429168034911115 44-45 20.78712647213218 28.559856220403468 26.594694488952232 24.058322818512128 46-47 22.464009219205092 28.13857549002199 26.606312848855197 22.791102441917726 48-49 21.387859564123644 27.641330746246663 28.927398716998454 22.04341097263124 50-51 22.063451636679506 26.702497026913047 26.714442005868598 24.51960933053885 52-53 20.4750518151791 27.90945434771464 26.238437708267725 25.377056128838532 54-55 22.297548461287736 27.882888120621267 28.462262820771063 21.357300597319934 56-57 22.002881376028725 27.210897589376902 26.44211863083823 24.344102403756136 58-59 20.498526005474837 26.4258379338442 27.29508271764307 25.780553343037894 60-61 20.851316598167166 26.266794395058422 29.73597076439033 23.14591824238408 62-63 20.35594772208058 26.207292372029013 29.572115330705138 23.864644575185263 64-65 23.135033201321416 28.13080508198203 27.528226558539714 21.205935158156837 66-67 21.903384809490305 30.119663409750242 26.01352696901679 21.963424811742662 68-69 21.230413421693058 27.600343159964915 28.424320775827184 22.744922642514844 70-71 20.947699536835472 30.862565529516957 26.705667344680567 21.484067588967008 72-73 23.372358338453143 30.291792525474676 25.98229385174191 20.35355528433027 74-75 24.74172995602862 28.13206797341572 26.449627039925865 20.67657503062979 76-77 23.34741003813779 27.06863131720833 26.540749165113546 23.043209479540334 78-79 23.358374687498 28.73812355077339 26.262892567052333 21.640609194676273 80-81 22.606290153403137 29.125631646174238 26.41185607793585 21.856222122486777 82-83 22.111866741026937 28.734675495003454 27.01309947532071 22.140358288648898 84-85 22.765530264107348 28.285802493575424 26.52514023758516 22.423527004732065 86-87 20.321790603761983 29.083049921334432 27.935354365910626 22.659805108992963 88-89 19.77641530744497 29.28984190100544 28.4553472586661 22.47839553288349 90-91 21.279309693699467 28.63468812048317 26.844652472205215 23.24134971361215 92-93 21.764093518971958 28.642457452091318 25.795444477031097 23.79800455190562 94-95 22.29740032979558 29.69909473357399 25.798124075855732 22.205380860774703 96-97 21.25550824671726 31.232402741343563 24.490713958513567 23.021375053425615 98-99 21.358131178973565 29.081384584533993 26.41345522570508 23.147029010787364 100-101 22.7857605592856 29.800546191652376 25.94030349576392 21.473389753298093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 232.0 1 161.5 2 90.5 3 144.5 4 178.5 5 139.0 6 131.0 7 144.5 8 158.5 9 172.5 10 185.5 11 195.5 12 205.0 13 223.0 14 241.5 15 265.0 16 278.0 17 286.0 18 319.5 19 353.0 20 374.5 21 424.0 22 482.5 23 529.5 24 603.0 25 763.5 26 995.5 27 1302.0 28 1739.5 29 2252.5 30 2918.5 31 3902.0 32 5525.0 33 8174.5 34 12968.0 35 22624.5 36 35025.5 37 49010.0 38 65986.0 39 80054.5 40 90424.0 41 97808.5 42 131714.5 43 168649.5 44 159272.0 45 136419.0 46 130849.5 47 126699.5 48 112740.5 49 101830.0 50 92336.5 51 87388.5 52 86292.5 53 86503.0 54 73483.5 55 46906.5 56 31130.5 57 25009.5 58 21673.5 59 18007.5 60 14156.0 61 11224.5 62 8613.0 63 6420.5 64 4922.5 65 3701.5 66 2801.5 67 2240.0 68 1795.0 69 1321.5 70 930.0 71 706.0 72 479.5 73 293.0 74 194.0 75 142.0 76 98.5 77 60.0 78 39.0 79 32.0 80 26.5 81 18.0 82 14.0 83 9.5 84 6.5 85 5.5 86 2.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15560384359800022 2 0.0 3 0.002059462635855885 4 0.010434610688336485 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.28829181761765E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.5765836352353E-5 22-23 6.86487545285295E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.864875452852951E-4 38-39 0.002059462635855885 40-41 0.006247036662096185 42-43 0.0026773014266126506 44-45 0.0011441459088088251 46-47 0.001464506763275296 48-49 0.0037299156627167702 50-51 8.009021361661775E-4 52-53 0.015926511050618847 54-55 0.028008691847640037 56-57 0.030342749501610044 58-59 0.02269985483076709 60-61 0.03265392423740387 62-63 0.006247036662096185 64-65 0.01183046869708325 66-67 0.03015968615620063 68-69 0.028924008574687098 70-71 0.032722572991932396 72-73 0.03249374381017064 74-75 0.04064006268088947 76-77 0.0209378701312015 78-79 0.011441459088088251 80-81 0.016818944859489728 82-83 0.00812343595254266 84-85 0.011967766206140311 86-87 0.0361321278001827 88-89 0.01649858400502326 90-91 0.022814269421647975 92-93 0.05105179045104978 94-95 0.05761918796761244 96-97 0.04231051570775035 98-99 0.03725339079081535 100-101 0.03757375164528182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2185036.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.792104946416444 #Duplication Level Percentage of deduplicated Percentage of total 1 86.62205126683509 37.06739908484272 2 5.747951302526038 4.919338707291705 3 2.3603871648747665 3.0301780582048607 4 1.1126898448621236 1.904573624566073 5 0.6944388971488443 1.4858251082833518 6 0.5034759575961132 1.2926877609270233 7 0.3737002358094382 1.1193993796480632 8 0.29737926725368496 1.0180387850566488 9 0.22708454417054258 0.8745683081269499 >10 1.6664122409696527 14.507180331906829 >50 0.21725706106279016 6.497579861754176 >100 0.1620400844418997 13.491285150213766 >500 0.010374469304707257 3.0589980712437215 >1k 0.004002471830772416 3.067218340698509 >5k 2.1576894672679442E-4 0.6689458770342235 >10k+ 5.39422366810143E-4 5.996783550201392 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 54550 2.4965263730208567 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 29901 1.3684442727717072 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24129 1.1042838653459257 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10917 0.49962563545863775 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10399 0.47591893222811893 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9105 0.4166979399881741 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5385 0.24644902875742095 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4817 0.2204540337092844 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4389 0.20086625575047734 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2731 0.12498649907827605 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2555 0.11693171188026193 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2512 0.11496378091711075 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2465 0.11281278660855015 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2283 0.1044834043924219 No Hit CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA 2216 0.10141709335681424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002471355163027062 0.0 0.0 2 0.0028832476901982395 0.0 0.0 3 0.0033866718900741224 0.0 0.0 4 0.003615501071835887 0.0 0.0 5 0.0038900960899500053 0.0 0.0 6 0.004256222780768829 0.0 0.0 7 0.004942710326054124 0.0 0.0 8 0.005583432034987066 0.0 0.0 9 0.006269919580272362 0.0 0.0 10-11 0.009908303570284426 0.0 0.0 12-13 0.013958580087467667 0.0 0.0 14-15 0.01734525197754179 0.0 0.0 16-17 0.021830303940072383 0.0 0.0 18-19 0.02636112173895533 0.0 0.0 20-21 0.0297020277926771 0.0 0.0 22-23 0.03356924096445093 0.0 0.0 24-25 0.037825463745219765 0.0 0.0 26-27 0.04215033528051712 0.0 0.0 28-29 0.04725322603380448 0.0 0.0 30-31 0.0963142026035269 0.0 0.0 32-33 0.10533007236494044 0.0 0.0 34-35 0.11203476739056015 0.0 0.0 36-37 0.11956324747052222 0.0 0.0 38-39 0.12855623431375957 0.0 0.0 40-41 0.1385789524749249 0.0 0.0 42-43 0.15091284537188404 0.0 0.0 44-45 0.16471124503211845 0.0 0.0 46-47 0.1796537906011617 0.0 0.0 48-49 0.1948709311883191 0.0 0.0 50-51 0.2137722216018409 0.0 0.0 52-53 0.2341151358604618 0.0 0.0 54-55 0.2572040003002239 0.0 0.0 56-57 0.28459485335710716 0.0 0.0 58-59 0.31642499254016865 0.0 0.0 60-61 0.3516646865314805 0.0 0.0 62-63 0.39289970508495053 0.0 0.0 64-65 0.4377273417920803 0.0 0.0 66-67 0.4835618268989619 0.0 0.0 68-69 0.5362383045405201 0.0 0.0 70-71 0.5966949743619785 0.0 0.0 72-73 0.6593255214101735 0.0 0.0 74-75 0.730560045692611 0.0 0.0 76-77 0.8136250386721318 0.0 0.0 78-79 0.905591486822185 0.0 0.0 80-81 1.0050864150522005 0.0 0.0 82-83 1.1205765030873631 0.0 0.0 84-85 1.2471648064379717 0.0 0.0 86-87 1.3909152984207127 0.0 0.0 88-89 1.5496312188906727 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 10975 0.0 56.4663 4 GAAGAGC 11060 0.0 55.898403 6 TCGGAAG 11150 0.0 55.666508 3 GATCGGA 11525 0.0 53.97921 1 ATCGGAA 11650 0.0 53.23662 2 GGAAGAG 13130 0.0 47.302773 5 TTGTGTT 22205 0.0 43.867096 1 GCCGTCT 7120 0.0 43.50556 50-51 ATGCCGT 7215 0.0 42.927315 48-49 TGTGTTT 41625 0.0 42.871464 1 TATGCCG 7185 0.0 42.77598 48-49 TCGTATG 7300 0.0 42.74208 44-45 TGCCGTC 7190 0.0 42.487312 50-51 TCTCGTA 7115 0.0 42.28389 42-43 GTATGCC 7385 0.0 42.22377 46-47 CGTATGC 7285 0.0 42.118774 46-47 CTCGTAT 7075 0.0 42.054043 44-45 TCGATCT 7145 0.0 41.810123 38-39 CGTCTTC 7435 0.0 41.66283 52-53 ACTCGAT 7340 0.0 41.572876 36-37 >>END_MODULE