FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l03n01_30230-1.34100000035714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l03n01_30230-1.34100000035714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185036
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT545502.4965263730208567TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA299011.3684442727717072No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC241291.1042838653459257No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC109170.49962563545863775No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT103990.47591893222811893No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT91050.4166979399881741No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC53850.24644902875742095No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT48170.2204540337092844No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT43890.20086625575047734No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC27310.12498649907827605No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT25550.11693171188026193No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC25120.11496378091711075No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC24650.11281278660855015No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA22830.1044834043924219No Hit
CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA22160.10141709335681424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA109750.056.46634
GAAGAGC110600.055.8984036
TCGGAAG111500.055.6665083
GATCGGA115250.053.979211
ATCGGAA116500.053.236622
GGAAGAG131300.047.3027735
TTGTGTT222050.043.8670961
GCCGTCT71200.043.5055650-51
ATGCCGT72150.042.92731548-49
TGTGTTT416250.042.8714641
TATGCCG71850.042.7759848-49
TCGTATG73000.042.7420844-45
TGCCGTC71900.042.48731250-51
TCTCGTA71150.042.2838942-43
GTATGCC73850.042.2237746-47
CGTATGC72850.042.11877446-47
CTCGTAT70750.042.05404344-45
TCGATCT71450.041.81012338-39
CGTCTTC74350.041.6628352-53
ACTCGAT73400.041.57287636-37
Produced by FastQC (version 0.11.2)