##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_53115004-1.3420000003588a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4725086 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.344934462568514 33.0 33.0 33.0 22.0 33.0 2 29.663317662366357 33.0 33.0 33.0 15.0 33.0 3 29.3098163715962 33.0 33.0 33.0 15.0 33.0 4 32.50231932286523 37.0 37.0 37.0 15.0 37.0 5 32.68532720885927 37.0 37.0 37.0 15.0 37.0 6 33.64302237038648 37.0 37.0 37.0 22.0 37.0 7 32.452335259083114 37.0 33.0 37.0 15.0 37.0 8 33.21335484687474 37.0 33.0 37.0 22.0 37.0 9 32.19432111923466 37.0 33.0 37.0 15.0 37.0 10-11 32.32015523103706 37.0 33.0 37.0 15.0 37.0 12-13 32.27173992177073 37.0 33.0 37.0 10.5 37.0 14-15 33.56570705803027 37.0 33.0 40.0 15.0 40.0 16-17 33.44882167224046 37.0 33.0 40.0 10.5 40.0 18-19 33.52950433071483 37.0 33.0 40.0 10.5 40.0 20-21 33.60908129502828 37.0 33.0 40.0 10.5 40.0 22-23 34.80958769850962 37.0 33.0 40.0 22.0 40.0 24-25 33.776860886764815 37.0 33.0 40.0 15.0 40.0 26-27 33.70470706776554 37.0 33.0 40.0 10.5 40.0 28-29 33.14786535102218 37.0 33.0 40.0 15.0 40.0 30-31 33.170178278236634 37.0 33.0 40.0 10.5 40.0 32-33 32.86962427773801 37.0 33.0 40.0 6.0 40.0 34-35 33.12588649180142 37.0 33.0 40.0 10.5 40.0 36-37 32.918096517185084 37.0 33.0 40.0 6.0 40.0 38-39 33.24982106145793 37.0 33.0 40.0 15.0 40.0 40-41 32.61982247518881 37.0 33.0 40.0 6.0 40.0 42-43 32.44634531519638 37.0 33.0 40.0 6.0 40.0 44-45 32.336464563819575 37.0 33.0 40.0 6.0 40.0 46-47 32.28369917499914 37.0 33.0 40.0 6.0 40.0 48-49 32.19972768749606 37.0 33.0 40.0 6.0 40.0 50-51 31.372804748950603 37.0 30.0 38.5 10.5 38.5 52-53 31.864541830561393 37.0 33.0 38.5 10.5 40.0 54-55 32.33975582666643 37.0 33.0 40.0 10.5 40.0 56-57 32.72048043146728 37.0 33.0 40.0 10.5 40.0 58-59 31.978485682588634 37.0 33.0 40.0 6.0 40.0 60-61 31.93855487498005 37.0 33.0 40.0 6.0 40.0 62-63 31.46034802329524 37.0 33.0 40.0 6.0 40.0 64-65 31.282531471384857 37.0 33.0 37.0 6.0 40.0 66-67 31.255400324988795 37.0 33.0 37.0 6.0 40.0 68-69 31.565371296945706 37.0 30.0 37.0 15.0 40.0 70-71 31.06848901797766 37.0 27.0 37.0 10.5 40.0 72-73 31.740592975450603 37.0 27.0 37.0 15.0 37.0 74-75 31.940023631315917 37.0 27.0 37.0 22.0 37.0 76-77 31.498052522218643 35.0 27.0 37.0 22.0 37.0 78-79 30.803287284083297 33.0 27.0 37.0 15.0 37.0 80-81 30.10290955550862 33.0 27.0 37.0 10.5 37.0 82-83 29.6918503705541 33.0 27.0 37.0 6.0 37.0 84-85 29.42901991625126 33.0 27.0 37.0 6.0 37.0 86-87 29.264838354264874 33.0 27.0 37.0 6.0 37.0 88-89 29.12179323296973 33.0 27.0 37.0 6.0 37.0 90-91 28.987672605324008 33.0 27.0 37.0 6.0 37.0 92-93 28.823722996787783 33.0 27.0 37.0 6.0 37.0 94-95 28.665914017226353 33.0 27.0 37.0 6.0 37.0 96-97 28.536860810575725 33.0 27.0 37.0 6.0 37.0 98-99 28.379816049908932 33.0 27.0 37.0 6.0 37.0 100-101 27.254664359548165 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.031201514028811772 2306 2 -0.1920703785211657 2306 3 -0.17216246129115476 2306 4 -0.05271944491287428 2306 5 -0.13163505177605117 2306 6 -0.18241493146016552 2306 7 0.33547828551920134 2306 8 -0.2775217275233217 2306 9 0.058578023092977105 2306 10-11 0.008300967588965591 2306 12-13 1.052464689692556E-4 2306 14-15 0.44128444571649794 2306 16-17 0.3116100806207669 2306 18-19 0.2958207535787807 2306 20-21 0.2592593242242103 2306 22-23 0.22326272076004017 2306 24-25 0.621766832117217 2306 26-27 0.1554477863201953 2306 28-29 0.27153205952793513 2306 30-31 0.3203510520662576 2306 32-33 0.4635146729021571 2306 34-35 0.2527909395143624 2306 36-37 0.18929070720402308 2306 38-39 0.29112519517647684 2306 40-41 0.37091559954636466 2306 42-43 0.35652040686287734 2306 44-45 0.4955343569881947 2306 46-47 0.3582743067270755 2306 48-49 0.3733576005481538 2306 50-51 0.4214659134046137 2306 52-53 0.324531123793772 2306 54-55 0.521952187097753 2306 56-57 0.7029105844038881 2306 58-59 0.9351745044090478 2306 60-61 0.5510472248468012 2306 62-63 0.6688299019009243 2306 64-65 0.617611941725535 2306 66-67 0.4453106429073941 2306 68-69 0.3737097426276961 2306 70-71 0.4875741618195839 2306 72-73 0.15979872713445076 2306 74-75 0.45683203850684606 2306 76-77 0.5857276060730392 2306 78-79 0.6449903906240699 2306 80-81 0.6427257711400678 2306 82-83 0.6679674211109798 2306 84-85 0.6811653629710861 2306 86-87 0.652224464215756 2306 88-89 0.5766215753462269 2306 90-91 0.46671225756536927 2306 92-93 0.5336440834703033 2306 94-95 0.5943332756131916 2306 96-97 0.677474093378784 2306 98-99 0.5752176192178204 2306 100-101 0.44883531596375903 2307 1 0.09343958600557656 2307 2 -0.14405724690403687 2307 3 0.01668080189332244 2307 4 0.05715669206988139 2307 5 -0.02390843273588672 2307 6 -0.13528743274688537 2307 7 0.618159132968394 2307 8 -0.24463457110709896 2307 9 0.17080028235769618 2307 10-11 0.0736167082210315 2307 12-13 0.137519006580618 2307 14-15 0.4649771524490447 2307 16-17 0.4101521829490977 2307 18-19 0.4714487685782487 2307 20-21 0.3238098835076073 2307 22-23 0.16101752432238925 2307 24-25 0.5869583306807016 2307 26-27 0.25826209409252954 2307 28-29 0.46737138879887397 2307 30-31 0.5639500509140305 2307 32-33 0.5126392127315498 2307 34-35 0.21837987112390778 2307 36-37 0.41339139648376744 2307 38-39 0.18314391528907947 2307 40-41 0.5289828624349084 2307 42-43 0.45445676916653355 2307 44-45 0.3896017810877268 2307 46-47 0.2658918457522432 2307 48-49 0.38755592751601853 2307 50-51 0.3825647421359797 2307 52-53 0.28659562006422945 2307 54-55 0.4434194856993301 2307 56-57 0.4937824801218369 2307 58-59 0.5263340110394736 2307 60-61 0.3034422698694179 2307 62-63 0.4384273128432632 2307 64-65 0.4696381195590682 2307 66-67 0.39379151474374297 2307 68-69 0.39585615965632925 2307 70-71 0.41657613334323784 2307 72-73 0.06505832706631054 2307 74-75 0.22425795337922239 2307 76-77 0.5432377776645616 2307 78-79 0.621377490182585 2307 80-81 0.5661202946245787 2307 82-83 0.47461515956738154 2307 84-85 0.534884427472381 2307 86-87 0.45043642652800386 2307 88-89 0.4892012617566017 2307 90-91 0.4182794864306345 2307 92-93 0.6350944914528505 2307 94-95 0.6326713501328634 2307 96-97 0.6680821259790264 2307 98-99 0.7059500286097027 2307 100-101 0.5589386554060631 2201 1 -0.07473271711043594 2201 2 -0.3011390262393796 2201 3 -0.13888195291998784 2201 4 -0.09766867528945511 2201 5 -0.16671116933576258 2201 6 -0.016261913241024217 2201 7 0.11879756894721538 2201 8 -0.27378647154313285 2201 9 -0.20593647520248481 2201 10-11 -0.18053864357878524 2201 12-13 -0.16903993226058134 2201 14-15 0.04072140283776804 2201 16-17 0.004085942868520931 2201 18-19 0.0050737001478609045 2201 20-21 -0.03222378482465871 2201 22-23 -0.09288814080608887 2201 24-25 0.23794422163288687 2201 26-27 -0.0074872932837593 2201 28-29 -0.03907642727044447 2201 30-31 0.21728912997663485 2201 32-33 0.0288749479346464 2201 34-35 -0.06355357384861549 2201 36-37 -0.0390819181869162 2201 38-39 -0.06491581341303743 2201 40-41 0.05655928560383927 2201 42-43 -0.0143535136373103 2201 44-45 0.06759076028096445 2201 46-47 0.016288474037757794 2201 48-49 0.0011042043225018006 2201 50-51 0.04498692993240283 2201 52-53 -0.07912213467427165 2201 54-55 0.291237581796544 2201 56-57 0.38981440393694555 2201 58-59 0.36602354853126684 2201 60-61 0.08811277100744164 2201 62-63 0.24267694178849908 2201 64-65 0.1324176999220441 2201 66-67 0.0969169806513257 2201 68-69 0.1084660050787356 2201 70-71 0.16976765002369731 2201 72-73 0.08878243401200336 2201 74-75 0.16224737188517935 2201 76-77 0.36244250280176615 2201 78-79 0.3876208390782736 2201 80-81 0.34119271490126124 2201 82-83 0.30499869548507164 2201 84-85 0.24782046789169598 2201 86-87 0.22066797085757983 2201 88-89 0.34125686855940884 2201 90-91 0.36178790356675705 2201 92-93 0.1960527385993629 2201 94-95 0.05507392166151348 2201 96-97 0.2714520365173456 2201 98-99 0.22222592803086272 2201 100-101 0.19025898854941303 1101 1 0.11036503532445963 1101 2 0.03332346079247728 1101 3 -0.0355670095761198 1101 4 0.015474446688543253 1101 5 0.06915774286787979 1101 6 0.12458991852361123 1101 7 0.23795521019598453 1101 8 0.10641809392143387 1101 9 0.030772994695951184 1101 10-11 0.1275028501995621 1101 12-13 0.18003231504155082 1101 14-15 0.26232174628059113 1101 16-17 0.29138234270615015 1101 18-19 0.28573452677260036 1101 20-21 0.1640944715508752 1101 22-23 0.2056605434668839 1101 24-25 0.3638492743239752 1101 26-27 0.26480822785958225 1101 28-29 0.33862616416445945 1101 30-31 0.502665890895031 1101 32-33 0.5160690582472185 1101 34-35 0.38759500234155553 1101 36-37 0.3702179570297659 1101 38-39 0.4126150857187767 1101 40-41 0.30652238626610284 1101 42-43 0.21445969206555304 1101 44-45 0.1771299256407488 1101 46-47 0.2334149971846884 1101 48-49 0.36219685363478504 1101 50-51 0.11957749769506165 1101 52-53 0.300033965584408 1101 54-55 0.5242476080331571 1101 56-57 0.5969000374745619 1101 58-59 0.5460938693046486 1101 60-61 0.41872996374076266 1101 62-63 0.5554630450854923 1101 64-65 0.5054060552576694 1101 66-67 0.491725338445935 1101 68-69 0.37563065026334286 1101 70-71 0.4083252891181921 1101 72-73 0.39322215456490284 1101 74-75 0.5385163961502428 1101 76-77 0.4986993602319423 1101 78-79 0.5430124655050115 1101 80-81 0.42473407704528654 1101 82-83 0.43020608140789207 1101 84-85 0.5575195934574495 1101 86-87 0.5420987083503981 1101 88-89 0.6289031934775586 1101 90-91 0.5577668607065647 1101 92-93 0.48407014324792996 1101 94-95 0.4266778604244301 1101 96-97 0.5052521112961195 1101 98-99 0.4752152595856529 1101 100-101 0.5298551656906909 2304 1 0.2739630786896754 2304 2 0.05439951795229092 2304 3 0.16750843799773918 2304 4 0.08244649494699985 2304 5 0.05255857395756891 2304 6 0.2580812402012356 2304 7 0.17478994710372575 2304 8 0.2015778821445764 2304 9 0.1174582690013537 2304 10-11 0.18571366393058497 2304 12-13 0.10820487054026984 2304 14-15 0.24410774482340258 2304 16-17 0.38332428415095166 2304 18-19 0.26569149154960314 2304 20-21 0.2897533311395364 2304 22-23 0.28269696797488564 2304 24-25 0.32275174921718275 2304 26-27 0.3772434961408635 2304 28-29 0.3304024560534913 2304 30-31 0.31752618553068856 2304 32-33 0.29679925355569026 2304 34-35 0.4318826908574849 2304 36-37 0.156283730875586 2304 38-39 0.3009645167933499 2304 40-41 0.2712775484786931 2304 42-43 0.17131345968013534 2304 44-45 0.21246489532791912 2304 46-47 0.1878524481294832 2304 48-49 0.2696626628343779 2304 50-51 0.23735298906566982 2304 52-53 0.30600538788602094 2304 54-55 0.5241531534810733 2304 56-57 0.6391020462386905 2304 58-59 0.4034161756438479 2304 60-61 0.4628424008944485 2304 62-63 0.39105464236439147 2304 64-65 0.43966780234130454 2304 66-67 0.45998198700712933 2304 68-69 0.40487403711111014 2304 70-71 0.39015042146032997 2304 72-73 0.5094432385499914 2304 74-75 0.6421358909075963 2304 76-77 0.5254792491195452 2304 78-79 0.5220424512844026 2304 80-81 0.353521414874546 2304 82-83 0.3070584988834 2304 84-85 0.24417112348323755 2304 86-87 0.3166516495290317 2304 88-89 0.42428699626013255 2304 90-91 0.3770291789321334 2304 92-93 0.38820608459377226 2304 94-95 0.38194245549215466 2304 96-97 0.5199493131788842 2304 98-99 0.5114260262273582 2304 100-101 0.2916186202219997 2202 1 0.001593707948138956 2202 2 -0.1891281740578563 2202 3 -0.2588141130206374 2202 4 -0.25318237050785086 2202 5 -0.27049014492024526 2202 6 -0.02541121487444542 2202 7 -0.14225570794808107 2202 8 -0.04561407271926754 2202 9 -0.25931927355187767 2202 10-11 -0.2196926648237536 2202 12-13 -0.09970551793030324 2202 14-15 -0.15961272948583627 2202 16-17 -0.06039495406070472 2202 18-19 -0.09991722453639795 2202 20-21 -0.2033046274860908 2202 22-23 -0.02300227350476547 2202 24-25 0.03218288015514048 2202 26-27 0.007878543714312514 2202 28-29 -0.02328680597668864 2202 30-31 -0.003521457713944187 2202 32-33 -0.07560620536428786 2202 34-35 -0.029005991544046594 2202 36-37 -0.05299500782219724 2202 38-39 -0.1110005489367083 2202 40-41 -0.1726387641281022 2202 42-43 -0.09135686657460695 2202 44-45 -0.2568895877761932 2202 46-47 -0.12726755280981905 2202 48-49 -0.1318353634863456 2202 50-51 -0.12481202115415257 2202 52-53 0.02845386561066121 2202 54-55 0.1188559209371931 2202 56-57 0.18730577468729592 2202 58-59 0.018034872157763004 2202 60-61 0.06124367665151098 2202 62-63 -0.0033311753239395614 2202 64-65 -0.09355060761975054 2202 66-67 -0.004748708426586035 2202 68-69 0.09115296269118645 2202 70-71 0.09798245308616416 2202 72-73 0.1770208884708495 2202 74-75 0.2163466719614533 2202 76-77 0.3041464391436186 2202 78-79 0.18288777242679188 2202 80-81 0.10160562671231688 2202 82-83 -0.06133551357737588 2202 84-85 0.0014468948945491888 2202 86-87 -0.13443109366827244 2202 88-89 -0.03313229618979108 2202 90-91 -0.10084801754639017 2202 92-93 -0.14035168307271917 2202 94-95 -0.06296738276822822 2202 96-97 -0.08133909839007458 2202 98-99 -0.1031092581615134 2202 100-101 -0.018961494392623734 1102 1 0.0712441448886949 1102 2 -0.1110026930941963 1102 3 5.729707766342074E-4 1102 4 -0.13661482436983619 1102 5 -0.13421768358574582 1102 6 0.10612325688548907 1102 7 -0.06335099951952827 1102 8 0.08957837642993383 1102 9 -0.06930068248217225 1102 10-11 -0.026293993849066055 1102 12-13 -0.02242513986765715 1102 14-15 -0.13485769588604057 1102 16-17 -0.0662648335557563 1102 18-19 0.0036900426984232126 1102 20-21 -0.011599744663435274 1102 22-23 0.0949142264278322 1102 24-25 0.07538044861517079 1102 26-27 0.1258283462339449 1102 28-29 0.16104467178779913 1102 30-31 0.15754046758424067 1102 32-33 -0.06177395593507384 1102 34-35 0.16732609336242632 1102 36-37 0.036458394561158514 1102 38-39 0.09751053087380512 1102 40-41 0.06816156205150037 1102 42-43 0.0637010401229503 1102 44-45 -0.017612490084708554 1102 46-47 0.1063622354653333 1102 48-49 -0.025449453107711406 1102 50-51 0.013254467792574331 1102 52-53 0.05801820408595049 1102 54-55 0.3671947056342404 1102 56-57 0.43866819546429525 1102 58-59 0.29078856622401617 1102 60-61 0.27905918085003023 1102 62-63 0.2882656797442671 1102 64-65 0.2623992511560509 1102 66-67 0.3403976451656874 1102 68-69 0.3204181882094055 1102 70-71 0.23763758157842574 1102 72-73 0.40221868726271737 1102 74-75 0.30102692466838477 1102 76-77 0.4713729917341105 1102 78-79 0.2502110086634026 1102 80-81 0.11883082597058703 1102 82-83 0.15653790527947464 1102 84-85 0.25625659780893884 1102 86-87 0.24376588834780222 1102 88-89 0.18218531496124157 1102 90-91 0.26572297826199787 1102 92-93 0.28170891914419727 1102 94-95 0.21441152318738688 1102 96-97 0.291043198856876 1102 98-99 0.283765568691571 1102 100-101 0.1619481093835482 2305 1 0.27985410040063385 2305 2 0.24682177570101516 2305 3 0.16511926830904144 2305 4 0.1638185102864682 2305 5 0.1321464726745134 2305 6 0.3921692314915717 2305 7 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-0.5772531559050655 1213 86-87 -0.7040814422257178 1213 88-89 -0.489759414342263 1213 90-91 -0.5359283479578991 1213 92-93 -0.42799947081142875 1213 94-95 -0.4483366711906491 1213 96-97 -0.5153406761630492 1213 98-99 -0.68077078911098 1213 100-101 -1.0295483496261078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2318.0 3 345.0 4 582.0 5 559.0 6 1056.0 7 6917.0 8 35897.0 9 119322.0 10 169752.0 11 126010.0 12 79253.0 13 52300.0 14 37359.0 15 28404.0 16 23986.0 17 21782.0 18 20724.0 19 20278.0 20 21011.0 21 21589.0 22 22701.0 23 23769.0 24 25988.0 25 30148.0 26 35324.0 27 42606.0 28 52619.0 29 63168.0 30 74439.0 31 95687.0 32 130078.0 33 184222.0 34 277095.0 35 458887.0 36 862225.0 37 1262941.0 38 293638.0 39 107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.259137029633536 22.134277664958585 22.31337504216145 13.293210263246433 2 29.251822101966486 29.58243116798103 29.476439546406734 11.689307183645747 3 34.52146684642767 20.543932451453298 30.418779289395864 14.515821412723163 4 26.113775208565986 17.476632345368746 33.602168196256514 22.807424249808758 5 37.85378240698453 15.947783033872955 29.022920846540977 17.17551371260154 6 34.066278346842 16.15481976068708 34.724241556439104 15.054660336031816 7 35.81090259606914 20.768595491438234 27.068430340404404 16.352071572088224 8 25.57766290291991 34.601331385240336 23.852922510327613 15.968083201512137 9 35.0474289942358 26.628888461346133 21.870118631814208 16.453563912603858 10-11 27.763877277675213 31.62061281502288 25.40718813810181 15.208321769200097 12-13 30.402278760562616 23.609049134344154 25.522974468315518 20.465697636777712 14-15 25.45315106207992 23.953426867823673 28.545799117209707 22.0476229528867 16-17 28.833690638705207 24.928811738474714 29.27666454243458 16.9608330803855 18-19 28.753213577320473 25.313623745584472 29.504692198992714 16.42847047810233 20-21 26.65102399834908 29.199562776509 27.52316155213068 16.62625167301124 22-23 31.617976904297795 25.022593713851382 26.69343725982591 16.665992122024914 24-25 24.50786314881086 32.07877590060425 26.513847288089536 16.89951366249535 26-27 22.58993346383636 33.94751765988681 26.375586119949357 17.086962756327473 28-29 24.58901226007589 31.522928828839643 26.15380872700857 17.734250184075893 30-31 28.97079113188699 26.762862578738115 26.649201183215443 17.617145106159445 32-33 23.26859628336925 26.35100560122658 29.28616163994255 21.094236475461624 34-35 18.81207933336375 26.18701341145985 36.691638290625136 18.309268964551258 36-37 22.007769203099617 26.84702139873244 28.858249711652423 22.28695968651552 38-39 19.537746722221396 26.203369792009372 26.059884065230822 28.198999420538414 40-41 22.93574418311954 25.641816380843064 28.708626749913886 22.713812686123514 42-43 25.795453628462834 25.860954828911158 30.863626267075013 17.479965275550992 44-45 21.70905496594383 29.4854951128955 31.406271191584985 17.39917872957569 46-47 24.686217340633778 30.7782412885446 27.240541565607252 17.294999805214374 48-49 19.677503898089313 28.848918030100073 31.16948199172434 20.30409608008627 50-51 18.859600144704036 26.407584308783466 32.199429166355294 22.533386380157204 52-53 27.914819318112094 25.94875851595572 26.439594329582057 19.69682783635013 54-55 29.731290433368017 26.0667255907586 26.570955347170305 17.63102862870308 56-57 29.85195503923495 25.944551390100106 26.219633354250714 17.98386021641423 58-59 26.686071177730764 25.94256535038032 26.309907987080592 21.06145548480832 60-61 20.76377973144545 26.300748748724956 25.646394829435643 27.289076690393955 62-63 25.349522042905775 26.53680609204282 26.84974913619655 21.263922728854855 64-65 20.49319285691999 29.17630395510709 31.015541142457252 19.314962045515667 66-67 19.27989139374839 28.770701952378214 28.22992058931966 23.71948606455373 68-69 18.989610546453694 26.30001885827123 36.28862005741107 18.421750537864 70-71 18.48790529212565 33.717813703070505 30.235231283424447 17.559049721379402 72-73 18.25267643690568 38.98682879555963 25.38074280904008 17.37975195849461 74-75 18.86095416135961 38.65812869449341 24.89833528640066 17.58258185774632 76-77 19.19274660388272 37.64873595392373 25.303678301302686 17.85483914089086 78-79 18.89135723098696 36.84299752918248 26.245439490432343 18.020205749398208 80-81 18.217941535954367 36.74968807292728 26.127913441298293 18.90445694982006 82-83 17.939177126272273 35.7080597164475 26.504417001262244 19.84834615601799 84-85 18.29582051592331 34.559420769830105 26.90647695838349 20.238281755863103 86-87 18.505875259252562 34.0193065080897 27.736609056637494 19.738209176020245 88-89 19.00907264752008 34.08818963664299 27.329743901371113 19.572993814465818 90-91 19.385759291136477 33.33682467591798 26.972881818902465 20.30453421404307 92-93 19.068535557146635 33.79398461840775 26.680581768980726 20.456898055464894 94-95 18.769204002293634 34.08261851806076 26.584688569338628 20.563488910306972 96-97 19.349594910434092 34.3271993224832 26.330339086207395 19.99286668087531 98-99 20.049404802643412 32.98909136439915 26.164936278309476 20.796567554647964 100-101 20.12777108460582 33.160816442948246 25.56107161585527 21.15034085659066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1889.0 1 1127.5 2 307.0 3 401.5 4 460.0 5 380.0 6 466.5 7 673.0 8 914.5 9 1126.0 10 1360.5 11 1595.0 12 1697.0 13 1664.5 14 1521.0 15 1453.0 16 1479.0 17 1400.0 18 1357.0 19 1446.0 20 1665.5 21 2045.5 22 2548.5 23 3256.0 24 4302.0 25 5623.5 26 7414.5 27 9781.0 28 12647.0 29 15996.5 30 20175.0 31 25418.0 32 33124.5 33 48288.5 34 72934.5 35 114413.0 36 167343.5 37 227708.5 38 294888.5 39 330404.5 40 328446.5 41 298540.5 42 268271.0 43 272541.0 44 269758.5 45 254875.0 46 256276.0 47 238362.5 48 193471.0 49 156616.5 50 132472.0 51 116418.0 52 102811.5 53 88233.5 54 74014.0 55 59336.5 56 46626.0 57 36585.0 58 28325.5 59 21478.5 60 16000.0 61 12057.5 62 8831.0 63 6193.0 64 4495.0 65 3232.0 66 2267.0 67 1646.0 68 1180.0 69 860.5 70 634.0 71 472.0 72 370.0 73 280.0 74 247.5 75 189.5 76 142.0 77 121.0 78 98.5 79 92.5 80 76.5 81 59.0 82 53.0 83 46.5 84 42.0 85 40.0 86 35.0 87 26.5 88 18.5 89 15.5 90 13.0 91 9.0 92 5.5 93 5.0 94 6.5 95 5.0 96 3.5 97 3.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17140428766799165 2 0.16363723326940505 3 0.13180712478037437 4 0.12605061579831564 5 0.1384736701088615 6 0.158663778817994 7 0.1349181792670017 8 0.12573316125886386 9 0.11872799775496148 10-11 0.10649541616808668 12-13 0.1350345792648007 14-15 0.13089708843394599 16-17 0.12134170679644772 18-19 0.11972268864524371 20-21 0.11223076151418196 22-23 0.12387076129408016 24-25 0.41934686479780475 26-27 0.30347595789790915 28-29 0.39083944715503594 30-31 0.22527632301295678 32-33 0.26364599501469393 34-35 0.27662988567827124 36-37 0.27282043120485006 38-39 0.29239679447104244 40-41 0.2856455945987015 42-43 0.3388721390467814 44-45 0.2879312672827542 46-47 0.3127879577218277 48-49 0.3124916668183394 50-51 0.31284086681173634 52-53 0.3165445031053403 54-55 0.2950210853305104 56-57 0.30666108511040857 58-59 0.30681981238013445 60-61 0.28553977641888423 62-63 0.2828731582874894 64-65 0.29174072175617544 66-67 0.29110581267727187 68-69 0.28836512182000495 70-71 0.26253490412661273 72-73 0.253191158848749 74-75 0.26271479503230205 76-77 0.258281013297959 78-79 0.25546624971481996 80-81 0.26340261320111424 82-83 0.3052642851368208 84-85 0.3110207941188795 86-87 0.3002590852314646 88-89 0.3068409760160979 90-91 0.28425937644309546 92-93 0.2687781767358309 94-95 0.2547784315460078 96-97 0.25380490429168906 98-99 0.2749897038911038 100-101 0.27485214025734134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4725086.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.95918058962191 #Duplication Level Percentage of deduplicated Percentage of total 1 91.16949188615818 43.72414125632329 2 4.192221660262066 4.021110313524661 3 1.3762284210294213 1.9800836213016066 4 0.6718570981001235 1.2888686359281258 5 0.4249882272483175 1.0191043569532665 6 0.2753085056531676 0.7922142204287527 7 0.20385405565570305 0.6843671428383088 8 0.17288024936255753 0.6632956079646212 9 0.13112784091525437 0.5659905422503707 >10 1.0608320862140062 10.206913212460124 >50 0.15249130512491782 5.142993468739907 >100 0.13516439671488262 13.066728629248242 >500 0.022825563152529805 7.666153603279764 >1k 0.010461569434256331 7.905142712880256 >5k 2.2261259280710998E-4 0.8073853090164612 >10k+ 4.4522381743074826E-5 0.465507366862037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 21801 0.4613884276392006 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9558 0.202282032538667 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8823 0.18672676010553035 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7373 0.15603948795852604 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6294 0.13320392475396214 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5764 0.121987197693333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006137454429400862 0.0 0.0 2 0.006666545328487143 0.0 0.0 3 0.007174472591609973 0.0 0.0 4 0.00797869075822112 0.0 0.0 5 0.008761745288868816 0.0 0.0 6 0.009481308911626159 0.0 0.0 7 0.010751127069433234 0.0 0.0 8 0.011788145231642344 0.0 0.0 9 0.013015636117522517 0.0 0.0 10-11 0.018856799643435063 0.0 0.0 12-13 0.024052472272462343 0.0 0.0 14-15 0.029089417631763738 0.0 0.0 16-17 0.0326237448376601 0.0 0.0 18-19 0.037046944754021406 0.0 1.0581817981725623E-5 20-21 0.04208389011332281 0.0 2.1163635963451247E-5 22-23 0.04696210820289832 0.0 2.1163635963451247E-5 24-25 0.052983162634500196 0.0 3.1745453945176874E-5 26-27 0.05928992615160867 0.0 4.2327271926902494E-5 28-29 0.06645381692523691 0.0 4.2327271926902494E-5 30-31 0.10238967079117714 0.0 4.2327271926902494E-5 32-33 0.11135247062169873 0.0 4.2327271926902494E-5 34-35 0.11800843413220416 0.0 4.2327271926902494E-5 36-37 0.12586014307464458 0.0 5.290908990862812E-5 38-39 0.13383883383286568 0.0 6.349090789035375E-5 40-41 0.14290745184320452 1.0581817981725623E-5 8.465454385380499E-5 42-43 0.15265330620437384 2.1163635963451247E-5 8.465454385380499E-5 44-45 0.16335152418389845 2.1163635963451247E-5 1.1639999779898186E-4 46-47 0.1747587239681987 2.1163635963451247E-5 1.269818157807075E-4 48-49 0.18709712373489074 2.1163635963451247E-5 1.269818157807075E-4 50-51 0.20051486893571885 2.1163635963451247E-5 1.269818157807075E-4 52-53 0.21524475956628092 2.1163635963451247E-5 1.269818157807075E-4 54-55 0.229974650196843 2.1163635963451247E-5 1.269818157807075E-4 56-57 0.24712777714522022 2.1163635963451247E-5 1.375636337624331E-4 58-59 0.2638787950102919 2.1163635963451247E-5 1.4814545174415873E-4 60-61 0.28307421282914214 2.1163635963451247E-5 1.5872726972588437E-4 62-63 0.3037405033474523 2.1163635963451247E-5 1.6930908770760997E-4 64-65 0.3250734483986112 2.1163635963451247E-5 1.6930908770760997E-4 66-67 0.34905184794520144 2.1163635963451247E-5 1.6930908770760997E-4 68-69 0.3749773020004292 2.1163635963451247E-5 1.6930908770760997E-4 70-71 0.40159057422446914 2.1163635963451247E-5 1.6930908770760997E-4 72-73 0.4315053736588075 2.1163635963451247E-5 1.6930908770760997E-4 74-75 0.4634413003276554 2.1163635963451247E-5 2.2221817761623812E-4 76-77 0.4995993723712119 2.1163635963451247E-5 2.3279999559796372E-4 78-79 0.5417679170283884 2.1163635963451247E-5 2.3279999559796372E-4 80-81 0.5874178798015528 2.1163635963451247E-5 2.3279999559796372E-4 82-83 0.6387714424668673 2.1163635963451247E-5 2.3279999559796372E-4 84-85 0.696706895916815 2.1163635963451247E-5 2.3279999559796372E-4 86-87 0.7573830402240298 2.1163635963451247E-5 2.3279999559796372E-4 88-89 0.8246516571338596 2.1163635963451247E-5 2.3279999559796372E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 11495 0.0 90.38361 2 GAGCGGC 6940 0.0 89.96851 9 CGGGAGA 10030 0.0 87.314316 4 AGAGCGG 7490 0.0 86.96717 8 TCGGGAG 10085 0.0 83.87614 3 AAGCGGC 2805 0.0 83.49459 9 GAGAGCG 8835 0.0 83.49365 7 GATCGGG 13275 0.0 80.2724 1 GAACCGG 1100 0.0 80.18959 1 CGGAAAG 3400 0.0 79.76945 5 ATCCGGA 1510 0.0 79.76512 2 ATTCGGA 1195 0.0 77.775795 2 TCGGGAA 3440 0.0 76.360855 3 TCGGAGA 1720 0.0 76.360855 4 CCGGAAA 1890 0.0 75.764305 4 GGATCGG 1980 0.0 75.20768 1 AAAGCGG 3390 0.0 74.39819 8 GAGCGTC 20890 0.0 74.34807 9 AGAGCGT 21165 0.0 74.32048 8 GATCCGG 1680 0.0 73.958786 1 >>END_MODULE