FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4725086
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT218010.4613884276392006Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC95580.202282032538667No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG88230.18672676010553035No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA73730.15603948795852604No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC62940.13320392475396214No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG57640.121987197693333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA114950.090.383612
GAGCGGC69400.089.968519
CGGGAGA100300.087.3143164
AGAGCGG74900.086.967178
TCGGGAG100850.083.876143
AAGCGGC28050.083.494599
GAGAGCG88350.083.493657
GATCGGG132750.080.27241
GAACCGG11000.080.189591
CGGAAAG34000.079.769455
ATCCGGA15100.079.765122
ATTCGGA11950.077.7757952
TCGGGAA34400.076.3608553
TCGGAGA17200.076.3608554
CCGGAAA18900.075.7643054
GGATCGG19800.075.207681
AAAGCGG33900.074.398198
GAGCGTC208900.074.348079
AGAGCGT211650.074.320488
GATCCGG16800.073.9587861
Produced by FastQC (version 0.11.2)