##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_52370180-2.342000000358cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5259731 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.952008762425304 33.0 33.0 33.0 27.0 33.0 2 30.446634438149022 33.0 33.0 33.0 22.0 33.0 3 30.25320439391292 33.0 33.0 33.0 22.0 33.0 4 33.57283842082418 37.0 37.0 37.0 15.0 37.0 5 33.67042782225935 37.0 37.0 37.0 22.0 37.0 6 34.37026057035997 37.0 37.0 37.0 27.0 37.0 7 33.63653103172006 37.0 37.0 37.0 22.0 37.0 8 34.042408632684825 37.0 37.0 37.0 27.0 37.0 9 33.3981426046313 37.0 37.0 37.0 15.0 37.0 10-11 33.449544853149334 37.0 37.0 37.0 15.0 37.0 12-13 33.421487809927925 37.0 37.0 37.0 15.0 37.0 14-15 34.93608789118683 40.0 37.0 40.0 15.0 40.0 16-17 34.874179877259884 40.0 37.0 40.0 15.0 40.0 18-19 34.978231681430096 40.0 37.0 40.0 15.0 40.0 20-21 34.97047624678905 40.0 37.0 40.0 15.0 40.0 22-23 35.86856542663493 40.0 37.0 40.0 27.0 40.0 24-25 35.050098569679704 40.0 37.0 40.0 22.0 40.0 26-27 34.953216333687024 40.0 37.0 40.0 18.5 40.0 28-29 34.56971202519673 40.0 37.0 40.0 15.0 40.0 30-31 34.600054261330094 40.0 37.0 40.0 15.0 40.0 32-33 34.3853320635599 40.0 37.0 40.0 15.0 40.0 34-35 34.704619875046845 40.0 37.0 40.0 18.5 40.0 36-37 34.28238820578467 37.0 37.0 40.0 15.0 40.0 38-39 34.26498151711561 37.0 37.0 40.0 15.0 40.0 40-41 34.868476638824305 37.0 37.0 40.0 18.5 40.0 42-43 34.42785942855252 37.0 37.0 40.0 15.0 40.0 44-45 33.94410408060793 37.0 35.0 40.0 15.0 40.0 46-47 33.80275027373073 37.0 33.0 40.0 10.5 40.0 48-49 33.72883137939944 37.0 33.0 40.0 15.0 40.0 50-51 32.849281075401 37.0 33.0 38.5 15.0 38.5 52-53 33.28893616422589 37.0 33.0 38.5 15.0 40.0 54-55 33.90897880518985 37.0 33.0 40.0 15.0 40.0 56-57 34.28074924364003 37.0 33.0 40.0 18.5 40.0 58-59 33.71361330075625 37.0 33.0 40.0 15.0 40.0 60-61 33.55420562382373 37.0 33.0 40.0 15.0 40.0 62-63 33.23341668994099 37.0 33.0 40.0 6.0 40.0 64-65 33.02888370146686 37.0 33.0 40.0 6.0 40.0 66-67 32.935849285828496 37.0 33.0 40.0 10.5 40.0 68-69 33.07559407505821 37.0 33.0 37.0 15.0 40.0 70-71 32.68363743316911 37.0 33.0 37.0 15.0 40.0 72-73 33.018972833401556 37.0 33.0 37.0 18.5 40.0 74-75 33.136925443525534 37.0 33.0 37.0 22.0 40.0 76-77 32.826356328869295 37.0 33.0 37.0 22.0 37.0 78-79 32.34977919973474 37.0 33.0 37.0 22.0 37.0 80-81 31.796122083049497 37.0 33.0 37.0 15.0 37.0 82-83 31.397389239107476 37.0 33.0 37.0 15.0 37.0 84-85 31.12071691499052 37.0 33.0 37.0 15.0 37.0 86-87 30.950733887341386 37.0 33.0 37.0 15.0 37.0 88-89 30.801294685982988 33.0 33.0 37.0 15.0 37.0 90-91 30.659256718642077 33.0 33.0 37.0 15.0 37.0 92-93 30.499360708751077 33.0 33.0 37.0 15.0 37.0 94-95 30.366471688381022 33.0 27.0 37.0 10.5 37.0 96-97 30.256860569485397 33.0 27.0 37.0 6.0 37.0 98-99 30.112651483507427 33.0 27.0 37.0 6.0 37.0 100-101 28.98764889307077 33.0 27.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.03743113548173582 2306 2 -0.10160381940699992 2306 3 -0.0653549381456422 2306 4 0.04239510125498924 2306 5 0.02104115110203253 2306 6 -0.11845547946541757 2306 7 0.5222732951865225 2306 8 -0.281710817843134 2306 9 0.04785291665017155 2306 10-11 0.051329193096613324 2306 12-13 0.08238050858176393 2306 14-15 0.40174832938821936 2306 16-17 0.26666971720353416 2306 18-19 0.30693047621474534 2306 20-21 0.22060045467129896 2306 22-23 0.2503828826000287 2306 24-25 0.583838247576125 2306 26-27 0.2243937364666877 2306 28-29 0.34227698027672915 2306 30-31 0.44203193183313516 2306 32-33 0.4716918257732914 2306 34-35 0.24057790342328644 2306 36-37 0.6211497443299479 2306 38-39 0.4207188930924204 2306 40-41 0.18685107602113504 2306 42-43 0.6795918785102302 2306 44-45 0.7005365116212303 2306 46-47 0.5450816166961161 2306 48-49 0.5707142359030044 2306 50-51 0.5052105369924291 2306 52-53 0.44044665630499225 2306 54-55 0.6508314516161917 2306 56-57 0.6473068598492873 2306 58-59 0.9148659184444412 2306 60-61 0.7016195740371245 2306 62-63 0.6981453784355764 2306 64-65 0.5484125410331941 2306 66-67 0.4652203690389598 2306 68-69 0.36416842732443655 2306 70-71 0.478015157354136 2306 72-73 0.11740454568788294 2306 74-75 0.2682613665198019 2306 76-77 0.5123457162270881 2306 78-79 0.4532164238230223 2306 80-81 0.5132667260676982 2306 82-83 0.6028183955061657 2306 84-85 0.6307673939411202 2306 86-87 0.7177551479913618 2306 88-89 0.6078683777868399 2306 90-91 0.5234259208384664 2306 92-93 0.5127067256410633 2306 94-95 0.5477051005867573 2306 96-97 0.5581589365103277 2306 98-99 0.6307838804081207 2306 100-101 0.5294731102306507 2307 1 0.11659335211215804 2307 2 0.012317260291347054 2307 3 0.06318784103008568 2307 4 0.1919390911442065 2307 5 0.09716117493676535 2307 6 0.02299001375800458 2307 7 0.6725355152327523 2307 8 0.04684615590450392 2307 9 0.24741622482039816 2307 10-11 0.24371915669930644 2307 12-13 0.275487829413251 2307 14-15 0.6251040931814345 2307 16-17 0.5032335681073974 2307 18-19 0.5424734944969032 2307 20-21 0.5127464158601498 2307 22-23 0.3940688030269257 2307 24-25 0.7005683723289451 2307 26-27 0.3438857681501801 2307 28-29 0.492097878178285 2307 30-31 0.6365986747861427 2307 32-33 0.573922423045957 2307 34-35 0.32816638228893424 2307 36-37 0.6191015511015863 2307 38-39 0.3581576078384501 2307 40-41 0.19097983672873653 2307 42-43 0.5975573485061645 2307 44-45 0.7123847663341039 2307 46-47 0.36499439978104675 2307 48-49 0.47415118202057016 2307 50-51 0.46437715621733844 2307 52-53 0.40411917918140716 2307 54-55 0.6124448506848097 2307 56-57 0.5666439277972586 2307 58-59 0.6169282303378907 2307 60-61 0.5304899669686804 2307 62-63 0.590701122493023 2307 64-65 0.6588905261630558 2307 66-67 0.5166113991322092 2307 68-69 0.3425348370465784 2307 70-71 0.38851735675371657 2307 72-73 0.09897942205467558 2307 74-75 0.3118851572295753 2307 76-77 0.43466656668689296 2307 78-79 0.5388704178491608 2307 80-81 0.6708530795429013 2307 82-83 0.6537067707590261 2307 84-85 0.6347246052770394 2307 86-87 0.6107445855599138 2307 88-89 0.6609333426230144 2307 90-91 0.6187329069209575 2307 92-93 0.6505477843891363 2307 94-95 0.6322069052709622 2307 96-97 0.7027722862395329 2307 98-99 0.4908992587465981 2307 100-101 0.5012652698171074 2201 1 0.09498692064315506 2201 2 -0.06632403257791708 2201 3 0.024895755203640846 2201 4 0.1741006498619484 2201 5 0.14739313689063493 2201 6 0.07349623079615242 2201 7 0.5669246265984214 2201 8 0.16429204618790294 2201 9 0.2326840657066711 2201 10-11 0.21329921140466723 2201 12-13 0.2025786342728466 2201 14-15 0.36275944110355596 2201 16-17 0.30612011327364286 2201 18-19 0.32258240581033704 2201 20-21 0.28205512866117743 2201 22-23 0.24119750003436735 2201 24-25 0.4790254065660946 2201 26-27 0.21031938042050058 2201 28-29 0.3705895584484651 2201 30-31 0.5079894789174446 2201 32-33 0.265611016593887 2201 34-35 0.23562204212939974 2201 36-37 0.4855393250575517 2201 38-39 0.3197224640678158 2201 40-41 0.16064791108557586 2201 42-43 0.529435444410133 2201 44-45 0.5148191443545684 2201 46-47 0.4204995295788123 2201 48-49 0.4303275917135423 2201 50-51 0.33998603683835427 2201 52-53 0.37220477257015716 2201 54-55 0.5445001303552814 2201 56-57 0.5562623948611645 2201 58-59 0.742620916903661 2201 60-61 0.5298284670347471 2201 62-63 0.4799961225398377 2201 64-65 0.5243737134732456 2201 66-67 0.3967431997167168 2201 68-69 0.3984737185311289 2201 70-71 0.403485275359472 2201 72-73 0.32029776083461314 2201 74-75 0.3417578408613551 2201 76-77 0.3943012331121878 2201 78-79 0.526680632498504 2201 80-81 0.5644797700976483 2201 82-83 0.6091689311524711 2201 84-85 0.5653070643962828 2201 86-87 0.5314254581405464 2201 88-89 0.5493419036265657 2201 90-91 0.5409374766598809 2201 92-93 0.6140064156731491 2201 94-95 0.5268147132174938 2201 96-97 0.49108242337747754 2201 98-99 0.522714201070837 2201 100-101 0.482430312906164 1101 1 0.11747276696319986 1101 2 -0.0035477107342387626 1101 3 0.035395818553446645 1101 4 0.057000870586001895 1101 5 0.01942919960027467 1101 6 0.057705458528694464 1101 7 0.1957494543869771 1101 8 -0.04467561873831016 1101 9 0.006493256096170796 1101 10-11 0.10662747675365125 1101 12-13 0.055596418896854516 1101 14-15 0.20561991816092728 1101 16-17 0.131165291686699 1101 18-19 0.14298837802978426 1101 20-21 0.0850859166660598 1101 22-23 0.10811556539802325 1101 24-25 0.30962269519447005 1101 26-27 0.24262311272423176 1101 28-29 0.1838169975826318 1101 30-31 0.22924300483418136 1101 32-33 0.20383028693751726 1101 34-35 0.1575224028898603 1101 36-37 0.31020573832500276 1101 38-39 0.1458179155518664 1101 40-41 0.03659565749963889 1101 42-43 0.16487865964609227 1101 44-45 0.016072803639318067 1101 46-47 0.08808443498755736 1101 48-49 0.10642004241739755 1101 50-51 0.053865645536610884 1101 52-53 0.07903188095166058 1101 54-55 0.29764594826794166 1101 56-57 0.3451695107112869 1101 58-59 0.44598645861116637 1101 60-61 0.25706554867597475 1101 62-63 0.4101446278178784 1101 64-65 0.40126037067077647 1101 66-67 0.2680750793387503 1101 68-69 0.22554942488145002 1101 70-71 0.34448926973134064 1101 72-73 0.18384494977039623 1101 74-75 0.33792084324196736 1101 76-77 0.4054868080283285 1101 78-79 0.4668304047726224 1101 80-81 0.5057404978868689 1101 82-83 0.42479432708874754 1101 84-85 0.4288475849316207 1101 86-87 0.38153315280782607 1101 88-89 0.418921327610132 1101 90-91 0.33872823559054055 1101 92-93 0.27239959790052737 1101 94-95 0.36056415473895953 1101 96-97 0.28402192098019086 1101 98-99 0.21512022663748454 1101 100-101 0.32556819542228155 2304 1 0.11637717124688862 2304 2 -0.03171864066915475 2304 3 -0.0023636068871795146 2304 4 -0.05582719130185154 2304 5 -0.034636083340835455 2304 6 0.1060983353083671 2304 7 0.08952620088538765 2304 8 0.06460487908500312 2304 9 -0.059798786326744846 2304 10-11 -0.02039804925889399 2304 12-13 -0.023624133502956113 2304 14-15 -0.014142018943964274 2304 16-17 0.01612690979730047 2304 18-19 0.08727036825111156 2304 20-21 0.019862117029965987 2304 22-23 0.11211223499403644 2304 24-25 0.21794297582074762 2304 26-27 0.2501850888255319 2304 28-29 0.24410673533642324 2304 30-31 0.21843009609963815 2304 32-33 0.0943434408319348 2304 34-35 0.20032012705220126 2304 36-37 0.05384197762986531 2304 38-39 0.0514443946661558 2304 40-41 0.2747403341667578 2304 42-43 0.2797388778596357 2304 44-45 0.006843592558794853 2304 46-47 0.054246920629580586 2304 48-49 0.17498653637249362 2304 50-51 0.13227318755247097 2304 52-53 0.0851654057555038 2304 54-55 0.32930483875549754 2304 56-57 0.4764051700601186 2304 58-59 0.3400242592508178 2304 60-61 0.26205459364289396 2304 62-63 0.30237741233685966 2304 64-65 0.3248056060399662 2304 66-67 0.31165457979663813 2304 68-69 0.35029070405598617 2304 70-71 0.29103462245937806 2304 72-73 0.3358063673544578 2304 74-75 0.4594051832277941 2304 76-77 0.5232356249679242 2304 78-79 0.4060148623945281 2304 80-81 0.3691934725641488 2304 82-83 0.3530657975212499 2304 84-85 0.30044701976095567 2304 86-87 0.29489727666854293 2304 88-89 0.4100348598251351 2304 90-91 0.370801208770807 2304 92-93 0.32974880332564993 2304 94-95 0.40073775343267215 2304 96-97 0.20424165001984917 2304 98-99 0.29384343624501597 2304 100-101 0.3641563406996049 2202 1 0.10890125449566668 2202 2 -0.016130982384868986 2202 3 0.06507406555196837 2202 4 0.07032776680003394 2202 5 -0.024045380452058396 2202 6 0.03132356574867856 2202 7 0.17097340931798755 2202 8 -0.02341520179755463 2202 9 0.06957664386663964 2202 10-11 0.027502772439753187 2202 12-13 0.09009850123629803 2202 14-15 0.15401618691981156 2202 16-17 0.12796770606404806 2202 18-19 0.06575324279879169 2202 20-21 0.15609748632022757 2202 22-23 0.13306969901629628 2202 24-25 0.2105867758399853 2202 26-27 0.11919630769001088 2202 28-29 0.22336237984570317 2202 30-31 0.28226019476466746 2202 32-33 0.1815366131995475 2202 34-35 0.13501593812298296 2202 36-37 0.1921919480934875 2202 38-39 0.04844447377900707 2202 40-41 0.13396700186988397 2202 42-43 0.15826745593147962 2202 44-45 0.10909546969735828 2202 46-47 0.03852033245279074 2202 48-49 0.14263207990675397 2202 50-51 0.10136247178510871 2202 52-53 0.21923561882819342 2202 54-55 0.3223024413878903 2202 56-57 0.33221118533855076 2202 58-59 0.2539564741433864 2202 60-61 0.18358991229542454 2202 62-63 0.17155840683782486 2202 64-65 0.22649696721936863 2202 66-67 0.24780253902953575 2202 68-69 0.2476188672126014 2202 70-71 0.30975418502868735 2202 72-73 0.32500834467966655 2202 74-75 0.2421154959330707 2202 76-77 0.3676841766651435 2202 78-79 0.377229746417008 2202 80-81 0.2716404304267712 2202 82-83 0.2560661043247592 2202 84-85 0.2205062910019997 2202 86-87 0.24052935117025243 2202 88-89 0.1645742700181252 2202 90-91 0.2190031554983598 2202 92-93 0.30419755625068845 2202 94-95 0.22416472385962294 2202 96-97 0.2250938691354456 2202 98-99 0.2267657686463629 2202 100-101 0.20192211647833957 1102 1 0.07876471837444043 1102 2 -0.06658779301609741 1102 3 0.04371365948666295 1102 4 0.005353081695773199 1102 5 -0.07899487903226543 1102 6 0.07764905471641725 1102 7 0.04899303221333895 1102 8 0.16758998698584548 1102 9 0.0029902522919300623 1102 10-11 0.05277273808749072 1102 12-13 0.05589210139445555 1102 14-15 -0.07638354324291896 1102 16-17 0.07322263938780083 1102 18-19 0.032996640643176534 1102 20-21 0.0786571085480432 1102 22-23 0.06087683468462757 1102 24-25 0.0772625141055272 1102 26-27 0.16756082036542352 1102 28-29 0.042650032543818384 1102 30-31 0.14656827781600867 1102 32-33 0.0673366832904847 1102 34-35 0.029832032851018653 1102 36-37 0.1745326580915858 1102 38-39 0.16632257331327338 1102 40-41 0.17193629664721755 1102 42-43 0.1033429898293079 1102 44-45 0.08088178091139042 1102 46-47 0.15953326846720728 1102 48-49 0.1326346537589771 1102 50-51 0.19691769856652286 1102 52-53 0.1686294156517718 1102 54-55 0.3745112045524053 1102 56-57 0.4634043166833166 1102 58-59 0.284923414584064 1102 60-61 0.20115340492944966 1102 62-63 0.3740785355176044 1102 64-65 0.41106110684668096 1102 66-67 0.34868791852190384 1102 68-69 0.2947468396589059 1102 70-71 0.18559838930397632 1102 72-73 0.2361533173878385 1102 74-75 0.33311192334915063 1102 76-77 0.4186867274104671 1102 78-79 0.28607461950876 1102 80-81 0.2966196342142524 1102 82-83 0.18950923823696186 1102 84-85 0.23657345121499418 1102 86-87 0.3403497270938871 1102 88-89 0.23648648175951337 1102 90-91 0.20119158458841113 1102 92-93 0.1669695116594987 1102 94-95 0.2751562025473575 1102 96-97 0.3546902172178328 1102 98-99 0.3311762345328688 1102 100-101 0.18148582606051278 2305 1 0.15820427333419218 2305 2 0.10149641945305987 2305 3 0.07028023743022871 2305 4 0.04075602704364201 2305 5 0.0699261962657971 2305 6 0.2342035546613701 2305 7 0.06352799688600186 2305 8 0.2536558460780185 2305 9 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78-79 -0.7376645717036041 1213 80-81 -0.7752384163543482 1213 82-83 -0.7106346881741352 1213 84-85 -0.7117340570598394 1213 86-87 -0.7752357242328429 1213 88-89 -0.6542216421259326 1213 90-91 -0.713119595801853 1213 92-93 -0.5705219427786226 1213 94-95 -0.5512359206566444 1213 96-97 -0.552999886042052 1213 98-99 -0.7657851881705717 1213 100-101 -1.1223803638668848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2631.0 3 345.0 4 528.0 5 520.0 6 918.0 7 5556.0 8 28107.0 9 86123.0 10 134833.0 11 111053.0 12 69477.0 13 45541.0 14 31553.0 15 24162.0 16 19698.0 17 18221.0 18 17311.0 19 17342.0 20 17744.0 21 18936.0 22 20284.0 23 20871.0 24 23374.0 25 26678.0 26 31276.0 27 37099.0 28 44624.0 29 54575.0 30 66621.0 31 86297.0 32 117291.0 33 167949.0 34 256206.0 35 436499.0 36 877796.0 37 1660592.0 38 680220.0 39 880.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.93072314971163 23.70823678906886 24.938511953078248 12.422528108141258 2 28.95059988573605 27.26564463911636 33.38838316511141 10.395372310036183 3 36.315971832219326 19.841532436924236 29.86593842153057 13.976557309325866 4 25.20544492587345 18.035674479370414 38.74090991033694 18.017970684419193 5 34.05522140041551 17.261704399373695 33.716156320634596 14.966917879576208 6 28.500537389922663 17.453670590260558 40.68613000799039 13.359662011826387 7 35.526315288473434 19.210379522717105 30.570721445365674 14.692583743443786 8 25.492648394159833 32.93598987881148 27.878365274241794 13.692996452786888 9 34.477458441061806 26.471101612372404 24.053028392350107 14.998411554215682 10-11 26.83042090519163 30.4573043975665 28.063549873240362 14.648724824001514 12-13 29.544171634194257 24.190445783841298 27.45809561831416 18.807286963650284 14-15 25.334032889948716 25.126469416322678 29.74902581474327 19.790471878985336 16-17 26.857830247859177 26.262235751221613 30.781855544588065 16.098078456331145 18-19 25.830424172745133 26.955993314958356 31.10137583943718 16.11220667285934 20-21 24.489487300326847 29.42559493672813 29.792431663264708 16.29248609968031 22-23 27.875623513345353 26.95506953815064 28.883590949546807 16.28571599895721 24-25 23.490944742451884 31.322246120095887 28.767805268287837 16.419003869164392 26-27 21.821702950131254 33.13710925469114 28.40356887787454 16.637618917303072 28-29 23.2640841034523 31.222211798407407 28.392626441536446 17.12107765660385 30-31 25.74627189181881 27.96205708662824 28.941394393777443 17.350276627775507 32-33 21.467866368501326 30.563974365304137 30.931121099518688 17.03703816667585 34-35 26.614469022296994 28.116587533878644 28.804913147125976 16.464030296698386 36-37 22.731277575031967 29.824458722706172 31.063852299585488 16.380411402676373 38-39 22.07999653723535 32.480150836197595 28.68277939109686 16.757073235470187 40-41 27.125463307972446 27.681447174906975 27.964892173735805 17.228197343384767 42-43 26.70931170443559 27.796937584940963 28.76820245270968 16.72554825791377 44-45 23.088889229980435 30.110634737574387 30.005231094341696 16.795244938103483 46-47 23.724292532379202 30.6900344086969 28.649400238952726 16.936272819971176 48-49 20.2685777582989 30.44606950679402 30.594745356251785 18.690607378655294 50-51 18.959001705699468 28.301918818927803 32.46743203020767 20.271647445165055 52-53 24.910313377489633 27.829673853389707 28.463857619059652 18.796155150061008 54-55 26.209340028191015 27.739814317618777 28.349685291856336 17.701160362333873 56-57 26.61694248742632 27.54384517866329 28.06448060180115 17.774731732109235 58-59 24.961769978708283 27.6179757431385 28.422844393681924 18.997409884471292 60-61 20.91482387898572 28.055666063868053 27.92142587278766 23.108084184358564 62-63 23.935442091052703 28.113156517694577 28.347234134720757 19.604167256531966 64-65 20.921573487910912 29.646270307375488 30.90877888795668 18.52337731675692 66-67 19.319739643203533 29.540748893323443 29.502117346279665 21.637394117193356 68-69 18.630434463437453 28.238378769929458 34.80104435814552 18.330142408487575 70-71 18.058999099777875 32.705036430640725 31.547300262918238 17.68866420666317 72-73 17.8913921013477 36.528293420991936 27.943763111336615 17.63655136632374 74-75 18.299951028823607 36.367760762936236 27.618085061160595 17.714203147079562 76-77 18.78346441195989 35.82590971686578 27.8260549661976 17.564570904976726 78-79 18.752028811167644 35.562412664623196 28.193560832121843 17.491997692087317 80-81 18.206377033384832 35.68278524671118 28.007590013459232 18.10324770644475 82-83 17.92121154736054 35.062693155343645 28.169340030545374 18.846755266750446 84-85 18.104182535901504 34.07704657344679 28.8520330611549 18.966737829496807 86-87 18.14915856454819 33.683925081656845 29.529971608895373 18.636944744899594 88-89 18.590822447747758 33.85291085914041 28.962583590712786 18.59368310239904 90-91 18.99350279472395 33.2444928480025 28.75628945162474 19.005714905648812 92-93 18.713451741667782 33.724919375793164 28.65216647828775 18.909462404251308 94-95 18.523231843822995 33.853713673588615 28.539976802942235 19.083077679646156 96-97 19.109963735881788 33.83208448583645 28.220786160641914 18.837165617639855 98-99 19.470173638817876 32.92334286157404 28.01826097326084 19.588222526347238 100-101 19.441507146190073 32.95010607160441 27.740372172873602 19.86801460933192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2491.0 1 1529.5 2 535.5 3 745.5 4 925.5 5 865.0 6 995.0 7 1259.0 8 1565.5 9 1914.0 10 2230.5 11 2463.0 12 2535.0 13 2444.5 14 2344.0 15 2407.5 16 2500.5 17 2580.5 18 2945.0 19 3576.5 20 4530.0 21 6037.0 22 8425.0 23 11862.5 24 16307.0 25 22227.0 26 30310.0 27 40422.5 28 51607.5 29 63677.5 30 76526.0 31 89839.5 32 105894.0 33 125782.0 34 151103.0 35 186520.5 36 234688.0 37 292649.0 38 345342.0 39 359032.5 40 348233.0 41 315914.0 42 270125.0 43 255145.0 44 240213.0 45 223439.0 46 229070.5 47 210824.5 48 158044.5 49 117064.5 50 95140.0 51 82138.5 52 72673.5 53 61962.0 54 53034.0 55 46202.0 56 39557.5 57 33704.5 58 28578.5 59 23741.0 60 19441.0 61 15822.5 62 12859.5 63 10378.0 64 8189.5 65 6358.5 66 5000.5 67 3917.0 68 3000.5 69 2287.0 70 1804.5 71 1354.5 72 979.0 73 732.5 74 568.5 75 422.0 76 318.5 77 236.5 78 168.0 79 127.0 80 100.5 81 87.0 82 72.5 83 58.5 84 45.5 85 35.5 86 30.0 87 28.5 88 27.0 89 20.5 90 16.0 91 18.0 92 11.5 93 8.0 94 5.5 95 1.5 96 3.0 97 2.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17333586071226836 2 0.16599708236029562 3 0.13158467609845445 4 0.12586194997424774 5 0.13968394961643477 6 0.16014126958203756 7 0.13462665676248461 8 0.12534861573719266 9 0.11726835459836255 10-11 0.10448252961986078 12-13 0.1353301147910416 14-15 0.13080516855329674 16-17 0.12024379193536705 18-19 0.11808588690182065 20-21 0.11207797509036109 22-23 0.1242554039360568 24-25 0.4214283962430778 26-27 0.3054528834269281 28-29 0.3927862470533189 30-31 0.224241505886898 32-33 0.26373972357141456 34-35 0.2764209804645903 36-37 0.273464555506736 38-39 0.2921537242113713 40-41 0.28470087158449736 42-43 0.33946602972661527 44-45 0.2876668027319268 46-47 0.31245894514377254 48-49 0.31265857512484957 50-51 0.3123258584897213 52-53 0.3158621610116563 54-55 0.29482496348197273 56-57 0.30683128091531675 58-59 0.30690733043191754 60-61 0.28570852767945737 62-63 0.28308481935673135 64-65 0.2914787847515396 66-67 0.2911270557372611 68-69 0.28841779170835924 70-71 0.26243737559962665 72-73 0.25318785314305997 74-75 0.26214268372279875 76-77 0.25817860266998444 78-79 0.25537427674533164 80-81 0.26409145258569305 82-83 0.3052722658250013 84-85 0.3109854857596329 86-87 0.3010610238432346 88-89 0.3075442451334488 90-91 0.28399741355594044 92-93 0.2680365212593572 94-95 0.2543095835129211 96-97 0.2540148916360932 98-99 0.27466233539319784 100-101 0.27479542204724916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5259731.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.173423404568894 #Duplication Level Percentage of deduplicated Percentage of total 1 93.86610979286043 57.42111277698402 2 3.078035911763465 3.7658798816954935 3 0.8712906558590552 1.598994965979315 4 0.5278872366774068 1.291706777565395 5 0.2992326805342515 0.9152543731402933 6 0.18717820060151635 0.6870198790501134 7 0.13113524511483124 0.5615394310870048 8 0.12187111097077968 0.5964218457760565 9 0.10842118900096917 0.5969245770704767 >10 0.6359096770247804 7.714559461050971 >50 0.07417854072327094 3.1451921880217317 >100 0.0806195346728593 10.185831177582493 >500 0.012268354431663924 5.236551337739302 >1k 0.005641636676705754 5.218178238850917 >5k 1.887719295728541E-4 0.8437558082408548 >10k+ 3.1461158205107986E-5 0.2210772801653894 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 11487 0.21839519929821505 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9027 0.1716247465887514 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8324 0.15825904404616892 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7890 0.1500076714949871 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6805 0.1293792401170326 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6493 0.1234473778221738 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5301 0.10078462187514912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016921017443667747 0.0 0.0 2 0.0019202502941690364 0.0 0.0 3 0.0020533369482203558 0.0 0.0 4 0.002224448360572052 0.0 1.901237915018848E-5 5 0.002585683564425633 0.0 3.802475830037696E-5 6 0.0028708692516784605 0.0 3.802475830037696E-5 7 0.003213092076381853 0.0 3.802475830037696E-5 8 0.0036313644176859997 0.0 3.802475830037696E-5 9 0.0039355624840890155 0.0 3.802475830037696E-5 10-11 0.006949024579393889 0.0 4.75309478754712E-5 12-13 0.009297053404442167 0.0 5.703713745056544E-5 14-15 0.011492983196288935 0.0 5.703713745056544E-5 16-17 0.01275730640977647 0.0 5.703713745056544E-5 18-19 0.014316321500091926 0.0 5.703713745056544E-5 20-21 0.015799287073806626 0.0 6.654332702565967E-5 22-23 0.017320277405821705 0.0 1.0456808532603663E-4 24-25 0.0188127491691115 0.0 1.1407427490113088E-4 26-27 0.02047633234475299 0.0 1.425928436264136E-4 28-29 0.023062015909178626 9.50618957509424E-6 1.5209903320150784E-4 30-31 0.04081957803545466 1.901237915018848E-5 1.5209903320150784E-4 32-33 0.043595385391382184 1.901237915018848E-5 1.9012379150188478E-4 34-35 0.046028969922606314 1.901237915018848E-5 1.9012379150188478E-4 36-37 0.04836749255807949 1.901237915018848E-5 1.9012379150188478E-4 38-39 0.050677496624827394 1.901237915018848E-5 2.186423602271675E-4 40-41 0.05366244015140698 1.901237915018848E-5 2.2814854980226175E-4 42-43 0.056904050796514116 1.901237915018848E-5 2.2814854980226175E-4 44-45 0.06034529142269823 1.901237915018848E-5 2.2814854980226175E-4 46-47 0.06400517440910952 1.901237915018848E-5 2.2814854980226175E-4 48-49 0.06871073824878116 1.901237915018848E-5 2.4716092895245024E-4 50-51 0.07434790866681205 1.901237915018848E-5 2.661733081026387E-4 52-53 0.07951927579566331 1.901237915018848E-5 2.661733081026387E-4 54-55 0.08542261952179683 1.901237915018848E-5 2.661733081026387E-4 56-57 0.09248571837609186 1.901237915018848E-5 3.0419806640301567E-4 58-59 0.10037585572342007 1.901237915018848E-5 3.0419806640301567E-4 60-61 0.10928315535528338 1.901237915018848E-5 3.0419806640301567E-4 62-63 0.11984453197321307 1.901237915018848E-5 3.0419806640301567E-4 64-65 0.13228813412701143 1.901237915018848E-5 3.2321044555320413E-4 66-67 0.1453686509823411 1.901237915018848E-5 3.2321044555320413E-4 68-69 0.1595994167762572 1.901237915018848E-5 3.2321044555320413E-4 70-71 0.1744005539446789 1.901237915018848E-5 3.517290142784869E-4 72-73 0.1922816965354312 1.901237915018848E-5 3.612352038535811E-4 74-75 0.21252037414080682 1.901237915018848E-5 3.8024758300376956E-4 76-77 0.2357820200310624 1.901237915018848E-5 3.8024758300376956E-4 78-79 0.26091638526761163 1.901237915018848E-5 3.8024758300376956E-4 80-81 0.29035705438167847 1.901237915018848E-5 3.897537725788638E-4 82-83 0.3225450122829475 1.901237915018848E-5 4.1827234130414653E-4 84-85 0.3594575464030385 1.901237915018848E-5 4.1827234130414653E-4 86-87 0.4009330515191747 1.901237915018848E-5 4.467909100294293E-4 88-89 0.4471236266645575 1.901237915018848E-5 4.7530947875471197E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5930 0.0 89.884705 9 CGGGAGA 8230 0.0 87.649414 4 AGAGCGG 6315 0.0 86.204575 8 TCGGGAG 8560 0.0 85.93255 3 ATTCGGA 1390 0.0 82.924225 2 GAGAGCG 7875 0.0 82.617004 7 ATCCGGA 1415 0.0 81.45914 2 GTTCGGA 905 0.0 79.66819 2 AAGCGGC 1930 0.0 79.36324 9 ATCGGGA 10770 0.0 79.32096 2 GATCGGG 11195 0.0 77.46032 1 CGGAGAG 2790 0.0 77.17405 5 CGGAAAG 3090 0.0 76.74166 5 TCGGAGA 1725 0.0 76.706955 4 AGAGCGT 14080 0.0 76.3166 8 GAGCGTC 14040 0.0 75.82692 9 AAGAGCG 13505 0.0 72.68447 7 GATCCGG 1695 0.0 71.646904 1 TTCGGAG 1270 0.0 71.32623 3 TCGGGAA 2415 0.0 70.894035 3 >>END_MODULE