FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5259731
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT114870.21839519929821505Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC90270.1716247465887514No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC83240.15825904404616892No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG78900.1500076714949871No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG68050.1293792401170326No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA64930.1234473778221738No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA53010.10078462187514912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC59300.089.8847059
CGGGAGA82300.087.6494144
AGAGCGG63150.086.2045758
TCGGGAG85600.085.932553
ATTCGGA13900.082.9242252
GAGAGCG78750.082.6170047
ATCCGGA14150.081.459142
GTTCGGA9050.079.668192
AAGCGGC19300.079.363249
ATCGGGA107700.079.320962
GATCGGG111950.077.460321
CGGAGAG27900.077.174055
CGGAAAG30900.076.741665
TCGGAGA17250.076.7069554
AGAGCGT140800.076.31668
GAGCGTC140400.075.826929
AAGAGCG135050.072.684477
GATCCGG16950.071.6469041
TTCGGAG12700.071.326233
TCGGGAA24150.070.8940353
Produced by FastQC (version 0.11.2)