FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556135
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT75620.11534234728235461Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA74540.11369503526086634No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG69120.10542796937524929No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC67640.10317054179024684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC24450.081.442639
AGAGCGG27200.075.4716348
CGGGAGA36150.074.3668444
AAGCGGC10950.067.5447859
CGGAAAG14150.066.345785
TCGGGAA12550.063.4702383
GAGAGCG38150.061.3904087
TCGGGAG42500.060.8011973
GAACCGG5800.053.1470951
ATTCGGA4200.053.063552
GATCCGG7800.052.8955081
GGAAAGC17750.049.951426
AAGCGTC5700.049.9065749
ACCGGAA6600.048.850563
TTGTGTT689900.048.6127551
GATCGGG70200.048.3693281
AACCGGA6300.047.4184882
GTTCGGA4700.045.400682
AAAGCGG18550.042.681278
CCGGATC18500.042.36951460-61
Produced by FastQC (version 0.11.2)