##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_52150223-1.3420000003573b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3167044 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.537381229941865 33.0 33.0 33.0 27.0 33.0 2 31.297451187921606 33.0 33.0 33.0 27.0 33.0 3 31.18548400337981 33.0 33.0 33.0 27.0 33.0 4 34.73951356533095 37.0 37.0 37.0 33.0 37.0 5 34.77836304137233 37.0 37.0 37.0 33.0 37.0 6 35.11269878157676 37.0 37.0 37.0 33.0 37.0 7 34.683314472422865 37.0 37.0 37.0 33.0 37.0 8 34.91552722349295 37.0 37.0 37.0 33.0 37.0 9 34.56532874188044 37.0 37.0 37.0 33.0 37.0 10-11 34.55182608767039 37.0 37.0 37.0 27.0 37.0 12-13 34.498237473176886 37.0 37.0 37.0 27.0 37.0 14-15 36.04567587314859 40.0 37.0 40.0 27.0 40.0 16-17 36.00025702200538 40.0 37.0 40.0 27.0 40.0 18-19 36.02994827353204 40.0 37.0 40.0 27.0 40.0 20-21 36.00654648309275 40.0 37.0 40.0 27.0 40.0 22-23 36.390589142430606 40.0 37.0 40.0 27.0 40.0 24-25 35.88818390271812 40.0 37.0 40.0 27.0 40.0 26-27 35.827859669774085 40.0 37.0 40.0 27.0 40.0 28-29 35.54576159977569 37.0 37.0 40.0 27.0 40.0 30-31 35.551833034211086 37.0 37.0 40.0 27.0 40.0 32-33 35.43096812043028 37.0 37.0 40.0 27.0 40.0 34-35 35.42822344747974 37.0 37.0 40.0 27.0 40.0 36-37 35.3298735350693 37.0 37.0 40.0 27.0 40.0 38-39 35.20278420508209 37.0 37.0 40.0 27.0 40.0 40-41 35.1490460820879 37.0 37.0 40.0 24.5 40.0 42-43 35.009828723566834 37.0 37.0 40.0 22.0 40.0 44-45 34.86662326131244 37.0 37.0 40.0 22.0 40.0 46-47 34.73395080712488 37.0 37.0 40.0 22.0 40.0 48-49 34.61739780059892 37.0 35.0 40.0 22.0 40.0 50-51 33.65219270714269 37.0 33.0 38.5 22.0 38.5 52-53 34.09952024664008 37.0 33.0 38.5 22.0 40.0 54-55 34.71498311990614 37.0 37.0 40.0 22.0 40.0 56-57 34.903549650715306 37.0 37.0 40.0 22.0 40.0 58-59 34.561781901356596 37.0 33.0 40.0 22.0 40.0 60-61 34.464524648220866 37.0 33.0 40.0 22.0 40.0 62-63 34.185429536185794 37.0 33.0 40.0 22.0 40.0 64-65 33.995687303365536 37.0 33.0 40.0 22.0 40.0 66-67 33.83332249251984 37.0 33.0 40.0 22.0 40.0 68-69 33.788069726849386 37.0 33.0 40.0 22.0 40.0 70-71 33.44472211311242 37.0 33.0 37.0 22.0 40.0 72-73 33.49762870361131 37.0 33.0 37.0 22.0 40.0 74-75 33.392040022178406 37.0 33.0 37.0 22.0 40.0 76-77 33.06637893253141 37.0 33.0 37.0 22.0 40.0 78-79 32.65112846553443 37.0 33.0 37.0 22.0 37.0 80-81 32.190825735291334 37.0 33.0 37.0 18.5 37.0 82-83 31.84460493760112 37.0 33.0 37.0 15.0 37.0 84-85 31.58975356831165 37.0 33.0 37.0 15.0 37.0 86-87 31.395053399952765 33.0 33.0 37.0 15.0 37.0 88-89 31.193276601146053 33.0 33.0 37.0 15.0 37.0 90-91 31.010821605257142 33.0 33.0 37.0 15.0 37.0 92-93 30.832477540570956 33.0 30.0 37.0 15.0 37.0 94-95 30.65042418103443 33.0 27.0 37.0 15.0 37.0 96-97 30.507519314540623 33.0 27.0 37.0 15.0 37.0 98-99 30.335658898329168 33.0 27.0 37.0 15.0 37.0 100-101 29.056915691730207 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2965848202569603 2306 2 0.16413568031566683 2306 3 0.1855906472782003 2306 4 0.3866499066865714 2306 5 0.21023890562178593 2306 6 0.24453059791104437 2306 7 0.48946826722370673 2306 8 0.15186884034618942 2306 9 0.33837246893802586 2306 10-11 0.3291956240113336 2306 12-13 0.33907389670538635 2306 14-15 0.6061836726284824 2306 16-17 0.454117177912984 2306 18-19 0.5402856109063165 2306 20-21 0.4196939417773393 2306 22-23 0.44969327562478867 2306 24-25 0.7561281560979438 2306 26-27 0.4827193727877841 2306 28-29 0.5912727642916096 2306 30-31 0.6983981202592418 2306 32-33 0.7222393081897351 2306 34-35 0.7031863763891764 2306 36-37 0.5976466150989239 2306 38-39 0.5756963184456083 2306 40-41 0.3826181138435132 2306 42-43 0.4605622875260096 2306 44-45 0.5917117532876759 2306 46-47 0.4695905718068545 2306 48-49 0.5642202928072706 2306 50-51 0.5221514252870705 2306 52-53 0.5206293568097493 2306 54-55 0.6454423548015527 2306 56-57 0.631888407274154 2306 58-59 0.6582156476648819 2306 60-61 0.760860500278774 2306 62-63 0.737224456957918 2306 64-65 0.8256396756233855 2306 66-67 0.6659911327517705 2306 68-69 0.7012741164819047 2306 70-71 0.6808335141434441 2306 72-73 0.4704469196707848 2306 74-75 0.5302036752996742 2306 76-77 0.6366377541643047 2306 78-79 0.6150318628562133 2306 80-81 0.5380321636341279 2306 82-83 0.662758617359092 2306 84-85 0.6334950267880366 2306 86-87 0.6096863863795292 2306 88-89 0.5410193492267972 2306 90-91 0.516101132929748 2306 92-93 0.3585510122089737 2306 94-95 0.42382505149173966 2306 96-97 0.5304409722114976 2306 98-99 0.6199394225921822 2306 100-101 0.2755225157778938 2307 1 0.14018583501799498 2307 2 -0.04395036761901494 2307 3 0.01833839337733778 2307 4 0.04203053517834121 2307 5 0.1688336503616199 2307 6 0.013091690647641485 2307 7 0.25203961158945276 2307 8 0.016145835949792797 2307 9 0.15940397571434062 2307 10-11 0.11393629298054009 2307 12-13 0.12688809604005513 2307 14-15 0.35089765628468683 2307 16-17 0.3810788148861377 2307 18-19 0.2748359319198457 2307 20-21 0.21428814330795376 2307 22-23 0.16714984296635294 2307 24-25 0.3512671399628289 2307 26-27 0.2344945946142616 2307 28-29 0.1337515961478104 2307 30-31 0.24216498719417956 2307 32-33 0.34952459126530044 2307 34-35 0.24838928043955377 2307 36-37 0.3224310490087916 2307 38-39 0.32091338060838126 2307 40-41 0.1987764689597924 2307 42-43 0.13355868988865183 2307 44-45 0.039390304421580424 2307 46-47 -0.038754527848929854 2307 48-49 0.143968670082117 2307 50-51 0.0651193701648154 2307 52-53 0.024941481226491646 2307 54-55 0.09337870557858707 2307 56-57 0.1788371574756198 2307 58-59 0.25746401562940235 2307 60-61 0.0352776258801768 2307 62-63 0.18295955835376532 2307 64-65 0.2962129539680234 2307 66-67 0.2813442733164635 2307 68-69 0.1423555009077333 2307 70-71 0.14908691674558128 2307 72-73 -0.004270415701832064 2307 74-75 -0.05616402083191474 2307 76-77 0.15032826360593532 2307 78-79 0.26570901230488175 2307 80-81 0.177415569812716 2307 82-83 0.28404468121764026 2307 84-85 0.35571735602546894 2307 86-87 0.20332400034270393 2307 88-89 0.38988968595871754 2307 90-91 0.26438385983998813 2307 92-93 0.3197414108898613 2307 94-95 0.3303447498886314 2307 96-97 0.26996974785260974 2307 98-99 0.050904980174603764 2307 100-101 0.17425541287442314 2201 1 0.04177953017603642 2201 2 0.08663204453132067 2201 3 0.03904855153803766 2201 4 0.09790808205776358 2201 5 0.12614353697030367 2201 6 0.08053556162374065 2201 7 0.2289015668934553 2201 8 0.13005413425982226 2201 9 0.1333415662406665 2201 10-11 0.12929743772985347 2201 12-13 0.11462211537680389 2201 14-15 0.32612716954174203 2201 16-17 0.26197773191599083 2201 18-19 0.1620266313713401 2201 20-21 0.17959726577186785 2201 22-23 0.2459789853757428 2201 24-25 0.5104744908628618 2201 26-27 0.3116338222236408 2201 28-29 0.23801472529907386 2201 30-31 0.40883273316136837 2201 32-33 0.17667164380349476 2201 34-35 0.24898991118277536 2201 36-37 0.27703054216926404 2201 38-39 0.22796132097919042 2201 40-41 0.23833067467015923 2201 42-43 0.3171188382277066 2201 44-45 0.3780278601944289 2201 46-47 0.19960845386987813 2201 48-49 0.19651684466114006 2201 50-51 0.26999111688319744 2201 52-53 0.20933053237023103 2201 54-55 0.29468498300630586 2201 56-57 0.36784831312585453 2201 58-59 0.5221899920186317 2201 60-61 0.2890832395380798 2201 62-63 0.22378733868588796 2201 64-65 0.3231611993491583 2201 66-67 0.38204328230272466 2201 68-69 0.35985074658891136 2201 70-71 0.42550063854076114 2201 72-73 0.4591806909050007 2201 74-75 0.5156958082500509 2201 76-77 0.5435965043244906 2201 78-79 0.44476928692211004 2201 80-81 0.3769256213385219 2201 82-83 0.2024797817976527 2201 84-85 0.30498780283430094 2201 86-87 0.4666431084950915 2201 88-89 0.45131996422398046 2201 90-91 0.4393171590674214 2201 92-93 0.33372671729248893 2201 94-95 0.34738231818137066 2201 96-97 0.4429643535085752 2201 98-99 0.35920474726155405 2201 100-101 0.4294749102225808 1101 1 0.03946777627759701 1101 2 -0.10632676137514707 1101 3 0.04242836516322157 1101 4 -0.0030633327466560445 1101 5 0.0240994646787982 1101 6 0.0017543175433587521 1101 7 -0.019025302969694735 1101 8 -0.06681460703891418 1101 9 0.00524382521227551 1101 10-11 -0.005342916260914876 1101 12-13 0.07262916098474648 1101 14-15 0.21027832183243333 1101 16-17 0.2218933078406451 1101 18-19 0.28596087052476804 1101 20-21 0.135969117915252 1101 22-23 0.23813083836512305 1101 24-25 0.2909174115073512 1101 26-27 0.26020695328003285 1101 28-29 0.10547922599149473 1101 30-31 0.29755313967694264 1101 32-33 0.2872466305159591 1101 34-35 0.35080455523336695 1101 36-37 0.3483447935711226 1101 38-39 0.18527847264827102 1101 40-41 0.07974610438603236 1101 42-43 -0.01370863909153286 1101 44-45 -0.01200980465826973 1101 46-47 0.09342869615345961 1101 48-49 0.11087735137110855 1101 50-51 -0.10455025652298389 1101 52-53 0.07326832099462877 1101 54-55 0.32437134218702113 1101 56-57 0.21462358195690712 1101 58-59 0.14663573200976288 1101 60-61 0.2673753738735982 1101 62-63 0.20555997759802125 1101 64-65 0.21312093216410943 1101 66-67 0.29377570920292584 1101 68-69 0.16385557975457488 1101 70-71 0.29208144969603467 1101 72-73 0.3737520049265157 1101 74-75 0.39432091238932543 1101 76-77 0.2783192961581378 1101 78-79 0.31939342001439996 1101 80-81 0.2257845951551971 1101 82-83 0.14445512676761396 1101 84-85 0.4097204154840277 1101 86-87 0.2397836362811958 1101 88-89 0.3016442442606895 1101 90-91 0.20272813631218867 1101 92-93 0.16375648319292324 1101 94-95 0.33325455009975613 1101 96-97 0.19641543241901616 1101 98-99 0.08607318510810913 1101 100-101 0.3680860739525045 2304 1 0.3493500238412395 2304 2 0.2727823148657329 2304 3 0.26427959069390994 2304 4 0.2656114779778349 2304 5 0.37051647508769037 2304 6 0.2790222822285813 2304 7 0.48847910874796696 2304 8 0.43859229421529733 2304 9 0.4485735754172566 2304 10-11 0.4500785775136009 2304 12-13 0.4628643766363467 2304 14-15 0.5095345415107744 2304 16-17 0.5989046545111663 2304 18-19 0.5749040565408379 2304 20-21 0.5232240413571105 2304 22-23 0.5165006917080959 2304 24-25 0.7206543166923467 2304 26-27 0.7079331932170021 2304 28-29 0.7305975985004096 2304 30-31 0.6980382862003083 2304 32-33 0.6882451445328925 2304 34-35 0.6010464492636274 2304 36-37 0.5722317414162248 2304 38-39 0.5353203878004322 2304 40-41 0.4666975647555063 2304 42-43 0.4915696464056438 2304 44-45 0.6061223439786048 2304 46-47 0.8088789323726218 2304 48-49 0.7972554965960441 2304 50-51 0.5903034918046828 2304 52-53 0.6638260839306014 2304 54-55 0.9099100231020643 2304 56-57 0.9607097847508683 2304 58-59 0.872528274968964 2304 60-61 0.7699286267128898 2304 62-63 0.968259633293421 2304 64-65 0.9205801202152486 2304 66-67 0.9383764109585471 2304 68-69 0.9321248235859443 2304 70-71 0.8151271146438788 2304 72-73 0.749280216913327 2304 74-75 0.8410760704946796 2304 76-77 0.9539947260787613 2304 78-79 0.8171703612906782 2304 80-81 0.8269416549332647 2304 82-83 0.7854610509802917 2304 84-85 0.7761460499409587 2304 86-87 0.844648642321971 2304 88-89 0.6891241905006176 2304 90-91 0.8233656629282002 2304 92-93 0.8742033035353352 2304 94-95 0.8948673040501482 2304 96-97 0.8131748762555624 2304 98-99 0.8884672348655336 2304 100-101 0.716894636300033 2202 1 0.25873646977139586 2202 2 0.2109408153942418 2202 3 0.15097531324636648 2202 4 0.22835831084501024 2202 5 0.11253129702721054 2202 6 0.15450590266760855 2202 7 0.10090869695739713 2202 8 0.18790755994061925 2202 9 0.14313745342245454 2202 10-11 0.2681700157614699 2202 12-13 0.2187745127366938 2202 14-15 0.3935525043840329 2202 16-17 0.24248611742036275 2202 18-19 0.19181502934868178 2202 20-21 0.2862559868748207 2202 22-23 0.24353471614912792 2202 24-25 0.37262300050728925 2202 26-27 0.25393223618776517 2202 28-29 0.15392445614536143 2202 30-31 0.31484612607737716 2202 32-33 0.36050902141065677 2202 34-35 0.2891808762003194 2202 36-37 0.38424203326948003 2202 38-39 0.41619787900132366 2202 40-41 0.3142280023699726 2202 42-43 0.2580690208619174 2202 44-45 0.22762846181296226 2202 46-47 0.36890248290674066 2202 48-49 0.3037529636475753 2202 50-51 0.3075905301577464 2202 52-53 0.4113093539394441 2202 54-55 0.5467521740853485 2202 56-57 0.4803953291180889 2202 58-59 0.4753021811545892 2202 60-61 0.44751540115286303 2202 62-63 0.4742046553089736 2202 64-65 0.36182087221325787 2202 66-67 0.5331544653798375 2202 68-69 0.5119704125184157 2202 70-71 0.45016129729599186 2202 72-73 0.44054901626303433 2202 74-75 0.4574312713978088 2202 76-77 0.5228612785352524 2202 78-79 0.47874898268432986 2202 80-81 0.4402731018087991 2202 82-83 0.5076090142065546 2202 84-85 0.35645998806083057 2202 86-87 0.4516838977044344 2202 88-89 0.39951869613993907 2202 90-91 0.31000902426329446 2202 92-93 0.3936146584710123 2202 94-95 0.37543571066300174 2202 96-97 0.37890567010373744 2202 98-99 0.4049252544951685 2202 100-101 0.4407736802244955 1102 1 0.19095489027375123 1102 2 -0.008654318284126106 1102 3 0.13315755675518304 1102 4 -0.0862724966371573 1102 5 0.03124031855732312 1102 6 -0.0015365804961788854 1102 7 -0.005139643705042829 1102 8 0.1641574312962888 1102 9 0.09323797037696124 1102 10-11 0.005576429231410884 1102 12-13 0.039294348305389803 1102 14-15 0.07852377132469712 1102 16-17 0.08239733396803928 1102 18-19 0.18674633576446098 1102 20-21 0.09340065485001503 1102 22-23 0.12438358513944081 1102 24-25 -0.03672598015270978 1102 26-27 0.029259298842220005 1102 28-29 0.07404796040140837 1102 30-31 0.09473193118736845 1102 32-33 -0.0471529569321163 1102 34-35 0.14041969802175913 1102 36-37 0.1378228296700854 1102 38-39 -0.006056317081537088 1102 40-41 0.007929789213100946 1102 42-43 -0.05926940292007288 1102 44-45 -0.009783360768743421 1102 46-47 0.07870925947042906 1102 48-49 0.17780777474644793 1102 50-51 0.03835811300933045 1102 52-53 0.1537817564377164 1102 54-55 0.26564597372017573 1102 56-57 0.2972860065566678 1102 58-59 0.24542283139245313 1102 60-61 0.3557554427289986 1102 62-63 0.33683705933317754 1102 64-65 0.31981997344664137 1102 66-67 0.26851331653004706 1102 68-69 0.19172089019280492 1102 70-71 0.21503069642766093 1102 72-73 0.285219895299214 1102 74-75 0.4469782340890305 1102 76-77 0.3345952219784394 1102 78-79 0.22093697977992122 1102 80-81 0.22299014989138755 1102 82-83 0.06701330303434005 1102 84-85 0.22838403616035663 1102 86-87 0.31892144866929684 1102 88-89 0.21946637579468486 1102 90-91 0.11491125955026149 1102 92-93 0.13567796130597287 1102 94-95 0.19942719956798882 1102 96-97 0.04006453244226904 1102 98-99 0.13750802137825602 1102 100-101 0.045895141117402005 2305 1 0.16213642659819172 2305 2 0.11473080591490614 2305 3 0.07525434085710359 2305 4 0.07109576011313834 2305 5 0.0646953873798708 2305 6 0.1941073952269292 2305 7 0.03331836507344832 2305 8 0.20166023647813347 2305 9 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-0.4801412958310074 1213 96-97 -0.4839594415455508 1213 98-99 -0.820012843961468 1213 100-101 -1.0940021194165084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1556.0 3 162.0 4 200.0 5 211.0 6 541.0 7 2761.0 8 10567.0 9 28848.0 10 42861.0 11 34818.0 12 23233.0 13 16550.0 14 12858.0 15 11168.0 16 10352.0 17 10480.0 18 10748.0 19 11755.0 20 12748.0 21 13993.0 22 15580.0 23 17219.0 24 19293.0 25 22487.0 26 26141.0 27 30829.0 28 38076.0 29 46094.0 30 55994.0 31 71621.0 32 94980.0 33 131617.0 34 195619.0 35 307895.0 36 538596.0 37 881950.0 38 415254.0 39 1389.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.453618016135174 25.55433824152663 20.883088935754074 16.108954806584123 2 30.49097282486319 26.864448160788967 29.126207144320947 13.518371870026893 3 35.85845652279756 21.939295874125122 26.242975845190696 15.959271757886622 4 29.599311826977974 21.38087948815709 30.606912459004576 18.412896225860358 5 33.28911095632577 19.73565852821058 29.917102013625257 17.0581285018384 6 29.850884091973523 20.158871863665816 33.298104371964705 16.69213967239596 7 33.42995434846736 21.34189390468729 26.857575375299607 18.370576371545745 8 28.699911797342537 29.754739707318922 24.058744866700177 17.48660362863836 9 31.095551126404153 26.62600347412661 24.200942977222184 18.077502422247054 10-11 26.973825613664665 29.974935046052508 27.666383250415645 15.384856089867185 12-13 27.82403105505424 26.995835149968432 27.40677144606894 17.773362348908393 14-15 25.539925725682654 27.23462770919232 28.603800595288437 18.62164596983659 16-17 26.168447949844932 27.592526118610905 29.007009905290648 17.232016026253515 18-19 25.58544569646301 27.763764112473982 29.408254871647593 17.242535319415417 20-21 24.528589355763547 29.277513837128073 28.660841006577968 17.53305580053041 22-23 26.260809114352075 28.111252986307793 27.956345925802044 17.67159197353808 24-25 23.198478233195637 30.79773700758616 28.154841896653593 17.848942862564606 26-27 22.80958785171827 31.020772179654482 28.133883069177944 18.0357568994493 28-29 23.44854781070093 30.396474653808188 27.985716160435963 18.16926137505492 30-31 24.754059320713036 28.405157511058647 28.597555427736854 18.243227740491463 32-33 24.575705476955086 28.474727377462127 28.701993952749383 18.247573192833404 34-35 22.28076324875308 28.47140951888807 28.2401532019667 21.00767403039215 36-37 20.93503200605134 28.39285199769877 28.77349872985829 21.898617266391604 38-39 20.738532495781335 29.662321671713332 29.85960168090992 19.73954415159541 40-41 21.76808244910264 28.521506534039453 30.93839497087327 18.772016045984643 42-43 22.60820428070918 28.33775742141039 30.30342442793658 18.750613869943848 44-45 21.371194776159577 29.568041978193886 30.261366191340255 18.79939705430628 46-47 22.695000753077522 30.113598299422673 28.220657573671332 18.970743373828476 48-49 20.640998250103856 29.523198041712746 29.85366535061511 19.98213835756829 50-51 20.368412950407787 28.412925067500193 30.624451224379385 20.594210757712634 52-53 23.721776580130605 28.330287917329823 28.22173310435564 19.726202398183933 54-55 23.94249644549594 28.353612625484402 28.680663418749635 19.02322751027002 56-57 24.216794029012103 28.25835047801065 28.445525852895898 19.079329640081344 58-59 23.026190658313098 28.6004573336583 28.20696161346073 20.16639039456787 60-61 20.93912465216066 28.422893469130866 28.13247780359497 22.505504075113507 62-63 22.713262797905312 28.474400231159873 28.401505705769893 20.41083126516492 64-65 20.845687333136883 29.520946393049023 29.928044499259038 19.705321774555053 66-67 20.24857706462199 29.399604083055785 28.817968460284643 21.53385039203758 68-69 20.067400811096118 28.65061733145166 31.724663063237664 19.557318794214563 70-71 20.02038215108626 30.829887236493104 29.693862496778685 19.455868115641955 72-73 19.920875607401193 32.57229459155732 28.007850709887776 19.498979091153707 74-75 19.78804963110986 32.62596148244954 28.136848363738594 19.449140522702002 76-77 19.89195561787891 32.577016326956375 27.962771873890496 19.56825618127422 78-79 19.815068477972865 32.52406930389354 28.074670953392307 19.58619126474129 80-81 19.77158082796093 32.17480075692266 28.225968069818734 19.82765034529768 82-83 19.90992009648726 32.045559064874254 27.99924809362913 20.045272745009356 84-85 19.907485347016262 31.704909044520456 28.23939465110861 20.148210957354667 86-87 20.129554137847872 31.318840283653444 28.27840679384863 20.273198784650052 88-89 20.2192962104676 31.364010900038693 28.17034770756008 20.24634518193363 90-91 20.28481583346013 31.105562892370276 28.182799680997654 20.426821593171944 92-93 20.321613449635105 31.09306780224477 28.108581741043864 20.476737007076256 94-95 20.234574212981357 30.954948138838812 28.288827018137557 20.521650630042277 96-97 20.389052708886325 30.81970145190419 28.157428643333 20.63381719587648 98-99 20.432221812183656 30.860594961211778 28.140598583482983 20.566584643121587 100-101 20.57318695709718 30.817454834221692 27.97742043546846 20.631937773212666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1290.0 1 747.0 2 168.5 3 142.5 4 182.0 5 228.5 6 311.0 7 434.0 8 581.5 9 798.0 10 1001.5 11 1105.5 12 1181.0 13 1230.5 14 1212.5 15 1172.0 16 1177.0 17 1249.5 18 1500.5 19 1948.0 20 2664.0 21 3803.0 22 5401.0 23 7699.5 24 10800.5 25 14658.5 26 19591.5 27 25459.5 28 32010.0 29 39689.0 30 47954.0 31 56760.0 32 66517.0 33 77346.5 34 89195.5 35 101907.0 36 115092.0 37 127599.5 38 138133.0 39 145771.0 40 149368.5 41 149303.5 42 147771.5 43 145317.5 44 142081.5 45 138524.0 46 133735.0 47 127643.0 48 119788.5 49 110117.0 50 100871.0 51 91928.0 52 81993.5 53 71293.0 54 60949.0 55 51114.5 56 42104.5 57 34360.0 58 28003.5 59 22482.5 60 17539.5 61 13553.5 62 10317.0 63 7708.5 64 5737.5 65 4336.0 66 3238.5 67 2399.0 68 1811.0 69 1392.5 70 1061.5 71 790.0 72 590.5 73 474.5 74 370.0 75 311.0 76 264.0 77 216.0 78 184.0 79 145.0 80 116.0 81 104.5 82 92.0 83 88.0 84 70.5 85 56.0 86 51.0 87 43.0 88 39.5 89 32.0 90 20.5 91 13.0 92 12.5 93 10.5 94 9.0 95 10.0 96 8.5 97 5.5 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16595917202287055 2 0.15901263133698174 3 0.12724799529150843 4 0.12270116866074485 5 0.1339103593129745 6 0.15386587619243686 7 0.13170009636746444 8 0.12232226644151455 9 0.11553360168030503 10-11 0.10306140363064106 12-13 0.1308791415591321 14-15 0.1275479595483991 16-17 0.11791752814296233 18-19 0.1156756900125164 20-21 0.10906068876845411 22-23 0.12178548829760497 24-25 0.412956056183621 26-27 0.30083257447638867 28-29 0.3880590228616969 30-31 0.22691506654154472 32-33 0.265815694382522 34-35 0.2797561385317034 36-37 0.2758092404147211 38-39 0.2952121915578059 40-41 0.28796568661502653 42-43 0.3422118543348308 44-45 0.2909337539989972 46-47 0.3157044865811779 48-49 0.31573606176611374 50-51 0.3162570523175554 52-53 0.3193356328488016 54-55 0.29776978153761047 56-57 0.3108419081010558 58-59 0.3103998555119537 60-61 0.28911818086518537 62-63 0.2859606623715995 64-65 0.29454911267415296 66-67 0.29399654693777544 68-69 0.29107584233120853 70-71 0.26516840309133693 72-73 0.255569546870836 74-75 0.26460004976249146 76-77 0.26047948812836197 78-79 0.2571798813025648 80-81 0.2672839404820394 82-83 0.30741600053551515 84-85 0.3133047725260527 86-87 0.3031059877917705 88-89 0.309673626258429 90-91 0.2864974405155091 92-93 0.2713571393387651 94-95 0.2566746783435911 96-97 0.2554116709461567 98-99 0.2780037157677633 100-101 0.2775458755861933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3167044.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.80224669077599 #Duplication Level Percentage of deduplicated Percentage of total 1 97.54832102864809 91.50251673400257 2 1.8075133794903622 3.390976318396663 3 0.2617123377679826 0.7364761580799596 4 0.11658752617691663 0.4374468756605774 5 0.06136944942395996 0.2878296117071696 6 0.02448984057562089 0.13783212402552997 7 0.03630362384677104 0.23837530358907683 8 0.02008793702234448 0.1507434899262983 9 0.020858305950351923 0.17609003642759322 >10 0.08919158807771327 1.695524036335042 >50 0.011772785546466588 0.7462853048674721 >100 0.001679949567450157 0.24693319450921508 >500 7.858006379129796E-5 0.05697793270867838 >1k 0.0 0.0 >5k 3.366784215794197E-5 0.19599287976415228 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 6206 0.1959555977119358 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017997855413439156 0.0 0.0 2 0.0019576614660232064 0.0 3.157518493585817E-5 3 0.002273413315381788 0.0 3.157518493585817E-5 4 0.0023681388701893625 0.0 3.157518493585817E-5 5 0.002747041089419661 0.0 3.157518493585817E-5 6 0.0033785447881368243 0.0 3.157518493585817E-5 7 0.004546826630763576 0.0 3.157518493585817E-5 8 0.005494082178839322 0.0 3.157518493585817E-5 9 0.0062203114323640596 0.0 6.315036987171633E-5 10-11 0.00931467955607816 0.0 6.315036987171633E-5 12-13 0.012266959347580899 3.157518493585817E-5 7.893796233964542E-5 14-15 0.015092938399340203 3.157518493585817E-5 9.472555480757451E-5 16-17 0.01791891745109951 3.157518493585817E-5 9.472555480757451E-5 18-19 0.020760684095326748 3.157518493585817E-5 1.2630073974343267E-4 20-21 0.02431289240061079 3.157518493585817E-5 1.2630073974343267E-4 22-23 0.028528179589547856 3.157518493585817E-5 1.5787592467929085E-4 24-25 0.03318551936758694 3.157518493585817E-5 1.8945110961514903E-4 26-27 0.03774813359081844 3.157518493585817E-5 1.8945110961514903E-4 28-29 0.04406317057799008 3.157518493585817E-5 1.8945110961514903E-4 30-31 0.0755120547741048 3.157518493585817E-5 1.8945110961514903E-4 32-33 0.0839742043369148 3.157518493585817E-5 2.2102629455100718E-4 34-35 0.09144173557424526 3.157518493585817E-5 2.5260147948686534E-4 36-37 0.10048802605836862 3.157518493585817E-5 2.841766644227235E-4 38-39 0.11130252689890005 3.157518493585817E-5 2.841766644227235E-4 40-41 0.12347476069167337 3.157518493585817E-5 2.841766644227235E-4 42-43 0.13806249613203986 3.157518493585817E-5 2.841766644227235E-4 44-45 0.15326594767865553 3.157518493585817E-5 2.9996425689065263E-4 46-47 0.16820101015331645 3.157518493585817E-5 3.473270342944399E-4 48-49 0.18430435447060414 3.157518493585817E-5 3.473270342944399E-4 50-51 0.20186015729494128 3.157518493585817E-5 3.473270342944399E-4 52-53 0.2211525952907506 3.157518493585817E-5 3.7890221923029806E-4 54-55 0.24213430568062838 6.315036987171633E-5 3.7890221923029806E-4 56-57 0.26488422642691417 6.315036987171633E-5 3.7890221923029806E-4 58-59 0.2895286582693515 6.315036987171633E-5 3.7890221923029806E-4 60-61 0.3161465391702799 6.315036987171633E-5 4.2626499663408525E-4 62-63 0.3449273202393146 6.315036987171633E-5 4.4205258910201436E-4 64-65 0.3762183285107501 6.315036987171633E-5 4.4205258910201436E-4 66-67 0.4100669267619901 6.315036987171633E-5 4.4205258910201436E-4 68-69 0.447925573500084 6.315036987171633E-5 4.4205258910201436E-4 70-71 0.48856283651253346 6.315036987171633E-5 4.4205258910201436E-4 72-73 0.5339837400427654 6.315036987171633E-5 4.4205258910201436E-4 74-75 0.5826411000289229 6.315036987171633E-5 4.4205258910201436E-4 76-77 0.6365399407144328 6.315036987171633E-5 5.052029589737307E-4 78-79 0.6982536396715675 6.315036987171633E-5 5.52565736377518E-4 80-81 0.7627933176804617 6.315036987171633E-5 5.68353328845447E-4 82-83 0.8377212315332531 9.472555480757451E-5 5.68353328845447E-4 84-85 0.9222322140140775 9.472555480757451E-5 6.630788836530215E-4 86-87 1.01293193274233 9.472555480757451E-5 6.630788836530215E-4 88-89 1.1133568084308272 9.472555480757451E-5 6.630788836530215E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 2845 0.0 86.498436 2 GATCGGG 3165 0.0 80.608315 1 GAGCGGC 2120 0.0 78.71569 9 AGAGCGG 2305 0.0 73.83534 8 TCGGGAG 2810 0.0 73.05961 3 GAGAGCG 2585 0.0 68.4052 7 CGGGAGA 3110 0.0 66.012054 4 GATCCGG 495 0.0 65.14408 1 ATCCGGA 530 0.0 60.835384 2 AAGCGGC 720 0.0 60.577293 9 CGGAAAG 1245 0.0 58.647175 5 TCGGGAA 1015 0.0 58.390167 3 AAGCGTC 450 0.0 57.943497 9 ATTCGGA 700 0.0 55.54424 2 TTCGGAA 620 0.0 55.06004 3 AAAGCGG 885 0.0 54.102695 8 TCGGAGA 535 0.0 54.059467 4 CGGGAAA 1170 0.0 49.439003 4 GAGCGTC 7290 0.0 48.123665 9 GTTCGGA 390 0.0 47.415813 2 >>END_MODULE