FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_52150223-1.3420000003573b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_52150223-1.3420000003573b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3167044
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT62060.1959555977119358Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA28450.086.4984362
GATCGGG31650.080.6083151
GAGCGGC21200.078.715699
AGAGCGG23050.073.835348
TCGGGAG28100.073.059613
GAGAGCG25850.068.40527
CGGGAGA31100.066.0120544
GATCCGG4950.065.144081
ATCCGGA5300.060.8353842
AAGCGGC7200.060.5772939
CGGAAAG12450.058.6471755
TCGGGAA10150.058.3901673
AAGCGTC4500.057.9434979
ATTCGGA7000.055.544242
TTCGGAA6200.055.060043
AAAGCGG8850.054.1026958
TCGGAGA5350.054.0594674
CGGGAAA11700.049.4390034
GAGCGTC72900.048.1236659
GTTCGGA3900.047.4158132
Produced by FastQC (version 0.11.2)