##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_50331580-1.342000000357a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3088702 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7033640668475 33.0 33.0 33.0 27.0 33.0 2 31.559584252543626 33.0 33.0 33.0 27.0 33.0 3 31.47465084038538 33.0 33.0 33.0 27.0 33.0 4 35.12885283203106 37.0 37.0 37.0 33.0 37.0 5 35.14382837839325 37.0 37.0 37.0 33.0 37.0 6 35.34108599664196 37.0 37.0 37.0 33.0 37.0 7 35.10353345839126 37.0 37.0 37.0 33.0 37.0 8 35.16902213292185 37.0 37.0 37.0 33.0 37.0 9 34.98516075684867 37.0 37.0 37.0 33.0 37.0 10-11 34.93363587681816 37.0 37.0 37.0 33.0 37.0 12-13 34.886980194269306 37.0 37.0 37.0 33.0 37.0 14-15 36.511174435086325 40.0 37.0 40.0 33.0 40.0 16-17 36.46630704418879 40.0 37.0 40.0 33.0 40.0 18-19 36.4940112383778 40.0 37.0 40.0 33.0 40.0 20-21 36.46898729628174 40.0 37.0 40.0 33.0 40.0 22-23 36.68377914088184 40.0 37.0 40.0 33.0 40.0 24-25 36.298522648024964 40.0 37.0 40.0 33.0 40.0 26-27 36.202835527674736 40.0 37.0 40.0 30.0 40.0 28-29 36.01276264268939 40.0 37.0 40.0 27.0 40.0 30-31 36.04728847263349 40.0 37.0 40.0 27.0 40.0 32-33 35.91073515670985 40.0 37.0 40.0 27.0 40.0 34-35 35.94945303884933 40.0 37.0 40.0 27.0 40.0 36-37 35.78167754610189 40.0 37.0 40.0 27.0 40.0 38-39 35.717137004476314 37.0 37.0 40.0 27.0 40.0 40-41 35.81696486096749 37.0 37.0 40.0 27.0 40.0 42-43 35.60187224277382 37.0 37.0 40.0 27.0 40.0 44-45 35.39051355553239 37.0 37.0 40.0 27.0 40.0 46-47 35.245074953815546 37.0 37.0 40.0 27.0 40.0 48-49 35.12700189270444 37.0 37.0 40.0 27.0 40.0 50-51 34.07433284272811 37.0 35.0 38.5 24.5 38.5 52-53 34.519362016795405 37.0 35.0 38.5 27.0 40.0 54-55 35.22369768919113 37.0 37.0 40.0 27.0 40.0 56-57 35.36059629579027 37.0 37.0 40.0 27.0 40.0 58-59 35.09119413268098 37.0 37.0 40.0 27.0 40.0 60-61 34.9178808768214 37.0 35.0 40.0 27.0 40.0 62-63 34.682398301940424 37.0 33.0 40.0 27.0 40.0 64-65 34.45849178716496 37.0 33.0 40.0 27.0 40.0 66-67 34.25596917410614 37.0 33.0 40.0 22.0 40.0 68-69 34.11216944852563 37.0 33.0 38.5 22.0 40.0 70-71 33.80304461226755 37.0 33.0 37.0 22.0 40.0 72-73 33.697614078664756 37.0 33.0 37.0 22.0 40.0 74-75 33.53672157430532 37.0 33.0 37.0 27.0 40.0 76-77 33.26083966663019 37.0 33.0 37.0 22.0 38.5 78-79 32.936628881646726 37.0 33.0 37.0 22.0 37.0 80-81 32.57172252292387 37.0 33.0 37.0 22.0 37.0 82-83 32.2850678051816 37.0 33.0 37.0 22.0 37.0 84-85 32.045000618382744 37.0 33.0 37.0 22.0 37.0 86-87 31.86908869810037 35.0 33.0 37.0 18.5 37.0 88-89 31.69148658562723 33.0 33.0 37.0 15.0 37.0 90-91 31.525533217513377 33.0 33.0 37.0 15.0 37.0 92-93 31.35411331361847 33.0 33.0 37.0 15.0 37.0 94-95 31.185171149563796 33.0 33.0 37.0 15.0 37.0 96-97 31.05060523805793 33.0 33.0 37.0 15.0 37.0 98-99 30.880604700615336 33.0 33.0 37.0 15.0 37.0 100-101 29.57154348331435 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2741129240932523 2306 2 0.10030045900216678 2306 3 0.10370608748273824 2306 4 0.22036526229329212 2306 5 0.23721918047127843 2306 6 0.14581937629827024 2306 7 0.35211938058138514 2306 8 0.15827573826346253 2306 9 0.21490420679428723 2306 10-11 0.24135687333222222 2306 12-13 0.19894461240988193 2306 14-15 0.5316700177580032 2306 16-17 0.46042960259480026 2306 18-19 0.45795936195676035 2306 20-21 0.4181101575175674 2306 22-23 0.42248796980759806 2306 24-25 0.6156218581292094 2306 26-27 0.4840342541577556 2306 28-29 0.41870953244316667 2306 30-31 0.4211001988870038 2306 32-33 0.42658907033512605 2306 34-35 0.4025141220271209 2306 36-37 0.44911280623879435 2306 38-39 0.25696352380820997 2306 40-41 0.3553973181216037 2306 42-43 0.39121237149554133 2306 44-45 0.38161050514683126 2306 46-47 0.3698281644581769 2306 48-49 0.48382744417533274 2306 50-51 0.3320111382296673 2306 52-53 0.5270456687494232 2306 54-55 0.7140666768119814 2306 56-57 0.6833014605551213 2306 58-59 0.6876458004589168 2306 60-61 0.653299017747706 2306 62-63 0.5883092846749349 2306 64-65 0.5996740000605101 2306 66-67 0.531232239982053 2306 68-69 0.5459977200523909 2306 70-71 0.506125528216117 2306 72-73 0.36458391614691976 2306 74-75 0.43122734719705846 2306 76-77 0.47848510671068567 2306 78-79 0.48880923372551166 2306 80-81 0.46131471850905115 2306 82-83 0.4820770084754855 2306 84-85 0.4395829268507683 2306 86-87 0.39645082206628146 2306 88-89 0.3613272959722629 2306 90-91 0.41848131943335076 2306 92-93 0.24159832413026194 2306 94-95 0.23498383825525693 2306 96-97 0.32559911931965857 2306 98-99 0.38905408339985925 2306 100-101 0.42259803487008796 2307 1 0.2354161372562409 2307 2 0.20779244553962783 2307 3 0.20027119361689927 2307 4 0.32838196891164273 2307 5 0.21537890409133809 2307 6 0.2154377123043858 2307 7 0.36706364299692495 2307 8 0.16602517963688257 2307 9 0.2764934461436468 2307 10-11 0.24401183968564055 2307 12-13 0.2987622990529175 2307 14-15 0.5849252951522033 2307 16-17 0.4887106469695084 2307 18-19 0.4549880001430182 2307 20-21 0.4603442612350861 2307 22-23 0.38140316917972683 2307 24-25 0.5458294948485971 2307 26-27 0.49725446422558406 2307 28-29 0.5390522753006266 2307 30-31 0.5911813243246087 2307 32-33 0.6199076737237448 2307 34-35 0.3782631623854229 2307 36-37 0.47604022914598687 2307 38-39 0.4222574823551213 2307 40-41 0.33269237731042267 2307 42-43 0.42684917061315275 2307 44-45 0.35314585760043116 2307 46-47 0.19687544242146515 2307 48-49 0.3279868504228105 2307 50-51 0.28457302295383613 2307 52-53 0.33596802073047627 2307 54-55 0.40449521579975567 2307 56-57 0.4091158710687566 2307 58-59 0.2358566320102895 2307 60-61 0.23912944541509518 2307 62-63 0.27224438823337493 2307 64-65 0.4314101453984023 2307 66-67 0.3907239279078638 2307 68-69 0.2926198711741179 2307 70-71 0.29040860815297265 2307 72-73 0.23011496609127846 2307 74-75 0.33916613728048617 2307 76-77 0.286074259290686 2307 78-79 0.4514218042598017 2307 80-81 0.34274615488971705 2307 82-83 0.38227651936210805 2307 84-85 0.32007006251230763 2307 86-87 0.3179671595240272 2307 88-89 0.39867769212126447 2307 90-91 0.3390299049951686 2307 92-93 0.28274996061200497 2307 94-95 0.2945046838108887 2307 96-97 0.46760263934129753 2307 98-99 0.23559984231316022 2307 100-101 0.2935363399050175 2201 1 0.2664173093849769 2201 2 0.27471918205016976 2201 3 0.2699261232710306 2201 4 0.3678177504094222 2201 5 0.26844512755060634 2201 6 0.3172343715560402 2201 7 0.40757030743787936 2201 8 0.3532057927001304 2201 9 0.3181853032963531 2201 10-11 0.3448985568905343 2201 12-13 0.3746081006184099 2201 14-15 0.5749895989331435 2201 16-17 0.4756989293110081 2201 18-19 0.5286481263555132 2201 20-21 0.4621418277071214 2201 22-23 0.36414475289621606 2201 24-25 0.59893382156406 2201 26-27 0.4617747079412666 2201 28-29 0.45327295484498364 2201 30-31 0.3899690964453697 2201 32-33 0.3985005386168581 2201 34-35 0.4357691409677571 2201 36-37 0.5079700320614293 2201 38-39 0.39107214448810623 2201 40-41 0.2598211699452406 2201 42-43 0.45529651735640897 2201 44-45 0.4886736965651792 2201 46-47 0.42380664245630584 2201 48-49 0.38190132494261064 2201 50-51 0.36451927921819305 2201 52-53 0.5193537309207414 2201 54-55 0.503553158151341 2201 56-57 0.5610607701314123 2201 58-59 0.6951761441434527 2201 60-61 0.49947883412999516 2201 62-63 0.4644281402791606 2201 64-65 0.5230842210315458 2201 66-67 0.4921586062256651 2201 68-69 0.4372761616588292 2201 70-71 0.39390848295656156 2201 72-73 0.41302039380939704 2201 74-75 0.2266506691729191 2201 76-77 0.31304192958501886 2201 78-79 0.29178458354760295 2201 80-81 0.3190713317069722 2201 82-83 0.3916214336660744 2201 84-85 0.3444017132542214 2201 86-87 0.3395824109873722 2201 88-89 0.3463281727666221 2201 90-91 0.3687662304622954 2201 92-93 0.37583657838554174 2201 94-95 0.2879276989034061 2201 96-97 0.27615065125854343 2201 98-99 0.2795773505053596 2201 100-101 0.2945555112862088 1101 1 0.33786967436260795 1101 2 0.24140619043522804 1101 3 0.3582450319659358 1101 4 0.43045147473081613 1101 5 0.4370596542347869 1101 6 0.3418514471362002 1101 7 0.44934522913349895 1101 8 0.4019699490189339 1101 9 0.4516270883327138 1101 10-11 0.4494096981957938 1101 12-13 0.4163987000472744 1101 14-15 0.616738391073369 1101 16-17 0.5098281745208766 1101 18-19 0.599717651202198 1101 20-21 0.48104630816727223 1101 22-23 0.49114770975041466 1101 24-25 0.6676677013141585 1101 26-27 0.6459622810618768 1101 28-29 0.6278045614923471 1101 30-31 0.6900363589782827 1101 32-33 0.6266385296417738 1101 34-35 0.5009623816732258 1101 36-37 0.7094395245411746 1101 38-39 0.7355618427001787 1101 40-41 0.6242940778107453 1101 42-43 0.6089128478228005 1101 44-45 0.41517547168916735 1101 46-47 0.6343602855906312 1101 48-49 0.6423578672775463 1101 50-51 0.4544273706143187 1101 52-53 0.5803819204965635 1101 54-55 0.8807899323818731 1101 56-57 0.8023284412968579 1101 58-59 0.946411482091321 1101 60-61 0.8708366269748851 1101 62-63 0.939928731987159 1101 64-65 0.9272841677609804 1101 66-67 0.772249402216211 1101 68-69 0.6296571204617933 1101 70-71 0.6170757896568517 1101 72-73 0.7044124753740704 1101 74-75 0.716489980836144 1101 76-77 0.7386551312478744 1101 78-79 0.8872080340649688 1101 80-81 0.760263817183997 1101 82-83 0.6351200631287526 1101 84-85 0.7675723915100505 1101 86-87 0.8188316667164877 1101 88-89 0.901669222466726 1101 90-91 0.7366848680841365 1101 92-93 0.7773873253536756 1101 94-95 0.6305310117938738 1101 96-97 0.6021697882077603 1101 98-99 0.5637851529591806 1101 100-101 0.6091454121287576 2304 1 0.23104491768282287 2304 2 0.11276811268610487 2304 3 0.15700141340135687 2304 4 0.20166533009167864 2304 5 0.08104444497060825 2304 6 0.2480116942053172 2304 7 0.16440473274136735 2304 8 0.07618012322715373 2304 9 0.13796760830611277 2304 10-11 0.04140008068781498 2304 12-13 0.15973357289410473 2304 14-15 0.35695737763921187 2304 16-17 0.30027248593167144 2304 18-19 0.3634744202302258 2304 20-21 0.2235182174745134 2304 22-23 0.2276055823425409 2304 24-25 0.2644385011945545 2304 26-27 0.3646379585509578 2304 28-29 0.1726314378802627 2304 30-31 0.060140722359051324 2304 32-33 0.06904829384867384 2304 34-35 0.20743801186949185 2304 36-37 0.02944542637145986 2304 38-39 0.06855258542532283 2304 40-41 0.23595657649977397 2304 42-43 0.330842023253723 2304 44-45 0.23504685608526898 2304 46-47 0.21030759123573972 2304 48-49 0.285024517441677 2304 50-51 0.3052452561566028 2304 52-53 0.2503106620843454 2304 54-55 0.35558533885577503 2304 56-57 0.5878938086271717 2304 58-59 0.5402411380719414 2304 60-61 0.32804216896288807 2304 62-63 0.39881606761044 2304 64-65 0.5402226278262319 2304 66-67 0.5703349878751496 2304 68-69 0.43306331034829526 2304 70-71 0.3951900962536712 2304 72-73 0.4420341029112578 2304 74-75 0.6405711746474267 2304 76-77 0.5695849561407016 2304 78-79 0.5360758402321864 2304 80-81 0.47404540516719607 2304 82-83 0.4233713820835874 2304 84-85 0.4085119746167507 2304 86-87 0.4735131991338868 2304 88-89 0.34361273556702443 2304 90-91 0.3506061522598678 2304 92-93 0.30940882599499275 2304 94-95 0.387092400925777 2304 96-97 0.5148376825767187 2304 98-99 0.5626865396287322 2304 100-101 0.47335511080319037 2202 1 0.17063578300202664 2202 2 0.18689930977854274 2202 3 0.15740150308727152 2202 4 0.271761542624958 2202 5 0.19661335546012282 2202 6 0.26646606972893494 2202 7 0.24758268787314108 2202 8 0.26638669762782285 2202 9 0.17328584490237375 2202 10-11 0.1589629746982837 2202 12-13 0.2814560478844541 2202 14-15 0.40769273160911723 2202 16-17 0.35904519061728735 2202 18-19 0.23218255811026722 2202 20-21 0.21855037598702154 2202 22-23 0.23846082510426214 2202 24-25 0.28514553004891496 2202 26-27 0.2232761931372167 2202 28-29 0.269954906938473 2202 30-31 0.32082177991232186 2202 32-33 0.2788171674882136 2202 34-35 0.2838472076730625 2202 36-37 0.40560238788791736 2202 38-39 0.24093092539547456 2202 40-41 0.28368780497640245 2202 42-43 0.22329408081374424 2202 44-45 0.32519339499359035 2202 46-47 0.274518973765268 2202 48-49 0.233437852549919 2202 50-51 0.30246409654593975 2202 52-53 0.4112799076324478 2202 54-55 0.4717481393601517 2202 56-57 0.49394215674016806 2202 58-59 0.488523485315703 2202 60-61 0.4772964858321487 2202 62-63 0.41517522502126525 2202 64-65 0.48836546528050206 2202 66-67 0.3629779659411909 2202 68-69 0.3876251408812905 2202 70-71 0.41629699439933177 2202 72-73 0.41185436669715614 2202 74-75 0.25320031487041206 2202 76-77 0.3832487315239135 2202 78-79 0.4941046984088402 2202 80-81 0.3563457195674502 2202 82-83 0.30196928739442797 2202 84-85 0.3496953379384493 2202 86-87 0.36540618902776956 2202 88-89 0.13739897395113232 2202 90-91 0.19323257134404415 2202 92-93 0.39393581654770315 2202 94-95 0.44688670810356257 2202 96-97 0.414330833740685 2202 98-99 0.37840409893765425 2202 100-101 0.4135465514404899 1102 1 0.07518916306299417 1102 2 0.07235137473869813 1102 3 0.08021304954868569 1102 4 0.012960895255687888 1102 5 0.08178939857383227 1102 6 0.11352394834267443 1102 7 0.02258792927158737 1102 8 0.13985255161377097 1102 9 0.01963179831651729 1102 10-11 0.021439237573645187 1102 12-13 0.04354708682766528 1102 14-15 0.07599120775736878 1102 16-17 0.07892902011119673 1102 18-19 0.01512908377954858 1102 20-21 -0.023014743298155338 1102 22-23 0.051025022808310894 1102 24-25 0.05578608173060928 1102 26-27 0.11205466071822912 1102 28-29 0.0724927619018203 1102 30-31 0.11665894059775894 1102 32-33 -0.1381834026552724 1102 34-35 -0.020626948084711216 1102 36-37 -0.005497649669159443 1102 38-39 -0.014041804249423251 1102 40-41 0.08920854356863117 1102 42-43 -0.13584163897732537 1102 44-45 -0.10843881117511955 1102 46-47 0.0019091268744304557 1102 48-49 -0.08864532806641279 1102 50-51 -0.21615587717919738 1102 52-53 -0.1134078332267805 1102 54-55 0.11341559789277511 1102 56-57 0.1273900466813629 1102 58-59 0.22454196067961618 1102 60-61 0.2282938598538209 1102 62-63 0.25490193322696086 1102 64-65 0.2343814368396977 1102 66-67 0.3111872930662898 1102 68-69 0.18355430884888335 1102 70-71 0.2577022698857263 1102 72-73 0.33991032824113177 1102 74-75 0.3690868804497285 1102 76-77 0.3270300196560285 1102 78-79 0.18593856191528602 1102 80-81 0.29621575551062307 1102 82-83 0.09644255324569428 1102 84-85 0.04447173761807477 1102 86-87 0.09395849969261505 1102 88-89 0.12828648222801675 1102 90-91 0.02174212124743491 1102 92-93 0.04166425479196434 1102 94-95 0.13755558596163198 1102 96-97 0.2233855569571368 1102 98-99 0.16615234891390074 1102 100-101 0.07048420217783047 2305 1 0.14679831126233367 2305 2 0.17621064501233263 2305 3 0.10815290401499666 2305 4 0.11444149681208415 2305 5 0.21850606172641562 2305 6 0.24746425389819393 2305 7 0.07890081246199543 2305 8 0.16206341239028177 2305 9 0.07702520552374637 2305 10-11 0.09105511811861078 2305 12-13 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12997.0 14 10256.0 15 9110.0 16 8770.0 17 9139.0 18 9429.0 19 10611.0 20 11503.0 21 12856.0 22 14409.0 23 16085.0 24 18224.0 25 20949.0 26 24094.0 27 29636.0 28 35789.0 29 42755.0 30 52643.0 31 67328.0 32 89264.0 33 125569.0 34 186551.0 35 301884.0 36 542462.0 37 910658.0 38 405164.0 39 2626.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.67499781914078 24.23374011357331 24.31163489206125 15.779627175224661 2 29.821074719033902 24.094648584444503 32.92207011027873 13.162206586242867 3 37.83588323599326 20.556655361019473 26.00372331062803 15.60373809235924 4 28.9622883392808 20.224974044323833 34.31166973034945 16.501067886045913 5 32.41455147140823 18.548827360127394 33.03170134575435 16.004919822710026 6 26.468700927249035 19.380402967637885 38.54356047157181 15.607335633541272 7 33.2438403324705 19.72020740505416 29.84882252733983 17.18712973513551 8 28.57026628681415 29.466295112213487 25.761630984451827 16.20180761652054 9 32.4472981382565 26.639559456516103 23.58560649276565 17.32753591246175 10-11 27.713967794138117 29.027373378416854 27.28722238093024 15.971436446514797 12-13 28.778159308932448 26.17847067509403 26.81950244627475 18.223867569698772 14-15 26.769931800213104 26.636281093725028 28.03953247212963 18.55425463393224 16-17 26.066076510103876 27.46091737511006 28.617445104921796 17.855561009864267 18-19 25.08980160105741 28.117863780958153 28.953883667039566 17.838450950944868 20-21 24.35215125291648 28.813983946101114 28.674880837055877 18.15898396392653 22-23 25.249263877417928 28.363960268180705 28.07818198791346 18.308593866487914 24-25 23.714430848880635 29.495044234121842 28.392706348013018 18.397818568984505 26-27 23.229141771270626 29.816723341576957 28.16390176233426 18.790233124818158 28-29 23.311941966552894 29.829296470562618 28.014906189520396 18.8438553733641 30-31 23.848322550321218 28.580949981285148 28.499665696068483 19.07106177232515 32-33 22.50972022155045 29.639424618068936 28.878638235711822 18.97221692466879 34-35 23.904020819643605 28.832388363232138 28.59147617922139 18.67211463790287 36-37 22.758926416303186 29.218206193167735 29.056094386178664 18.966773004350415 38-39 22.53900188996264 30.306519437586342 28.225384985926343 18.929093686524666 40-41 24.054816625837486 28.427662541539426 28.13554314175275 19.381977690870336 42-43 23.8572915972952 28.540039990449074 28.230511965460725 19.372156446794996 44-45 22.436447536362888 29.06488840228621 29.20320651251016 19.29545754884074 46-47 22.811800264788047 29.158041893541437 28.438635540999197 19.591522300671325 48-49 21.611462388590873 29.516228716444104 28.880096938077916 19.992211956887108 50-51 21.227704006081083 28.584015915264953 29.60346781554674 20.58481226310722 52-53 23.072899480082835 28.652005617495824 28.16649951022296 20.108595392198385 54-55 23.25402801712031 28.589204436785483 28.530041235647158 19.626726310447047 56-57 23.5567751887635 28.380368596249088 28.14708126978972 19.915774945197697 58-59 22.920213273820696 28.68521846667061 28.355217563848672 20.039350695660023 60-61 21.5437149310476 28.523600249292635 28.439101150372842 21.493583669286924 62-63 22.52102886614172 28.754177161145684 28.152633488555573 20.572160484157024 64-65 21.456625927032327 29.21845167742522 29.195884690816047 20.129037704726404 66-67 21.30699049655099 28.91569179575832 28.447175785882113 21.330141921808575 68-69 21.083130414341127 28.714951727223625 30.1332227648728 20.068695093562443 70-71 20.87412354172486 29.85100716520515 29.279585827867905 19.995283465202085 72-73 20.74569437481834 31.289146934191038 27.90754965894343 20.057609032047193 74-75 20.73728163933995 31.15062075283028 28.199332317207272 19.9127652906225 76-77 21.130147757891418 30.717402037079534 28.050239176209423 20.10221102881962 78-79 20.950590335537793 30.820789548423743 28.3628190043413 19.865801111697166 80-81 20.593885464946045 30.682134679220958 28.519402426934548 20.204577428898453 82-83 20.538221109622125 30.77029370726569 28.201164982584366 20.49032020052782 84-85 20.496543419050823 30.372136383484083 28.660206068182 20.471114129283094 86-87 20.77352126378945 29.991358810068203 28.67691399434377 20.55820593179857 88-89 20.878383929971946 30.46528296261752 28.433146933495884 20.223186173914655 90-91 21.033252444679366 29.89984590678952 28.383638197193857 20.68326345133726 92-93 20.894991111670915 30.211326678881356 28.14839354326844 20.74528866617929 94-95 20.55135574986047 30.23628025154367 28.588172306340056 20.62419169225581 96-97 20.93237779577746 30.23025438680056 28.17705174609455 20.66031607132743 98-99 21.051484196099874 30.146922160035437 27.98807818815211 20.813515455712572 100-101 21.116304468159225 29.802624295625925 27.99393679081701 21.08713444539784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1220.0 1 728.0 2 196.5 3 181.0 4 199.5 5 219.5 6 293.0 7 431.0 8 617.0 9 783.0 10 982.0 11 1169.5 12 1242.0 13 1210.5 14 1130.5 15 1046.5 16 1001.5 17 1024.0 18 1133.0 19 1428.5 20 1851.0 21 2386.5 22 3160.0 23 4404.5 24 6124.0 25 8366.5 26 11339.0 27 14882.5 28 19179.0 29 24337.5 30 30193.5 31 37194.5 32 45902.5 33 56250.0 34 69048.0 35 85351.0 36 105719.0 37 129223.5 38 152090.0 39 167790.0 40 173858.5 41 168832.0 42 159557.5 43 156236.0 44 152782.5 45 149077.5 46 148199.5 47 140512.5 48 124641.5 49 109942.5 50 99012.5 51 89321.0 52 78859.5 53 67657.0 54 56705.0 55 46568.5 56 37700.5 57 30158.0 58 24120.5 59 19321.5 60 15396.0 61 11921.5 62 9018.0 63 6855.0 64 5177.5 65 3957.5 66 2995.0 67 2245.5 68 1683.5 69 1224.5 70 941.5 71 727.0 72 559.0 73 463.0 74 359.5 75 280.0 76 228.0 77 191.0 78 162.5 79 129.0 80 117.5 81 99.5 82 72.0 83 64.0 84 57.5 85 44.0 86 42.5 87 43.5 88 33.5 89 24.5 90 23.0 91 16.5 92 16.0 93 15.5 94 9.0 95 7.0 96 6.5 97 5.0 98 3.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16366098121476272 2 0.15504894936449035 3 0.12344991520709994 4 0.1176869766005267 5 0.13073452861428522 6 0.14999828406884186 7 0.12681702540419892 8 0.1180107371964016 9 0.11079087590839128 10-11 0.09782426404360148 12-13 0.12657420495729274 14-15 0.12286714613452512 16-17 0.11252299509632202 18-19 0.11100132029570998 20-21 0.10428328793130577 22-23 0.11713658358753935 24-25 0.4124548111148308 26-27 0.29624094522553485 28-29 0.3883022706625631 30-31 0.224220400673163 32-33 0.26352493701237606 34-35 0.2762972925196409 36-37 0.2726226097564608 38-39 0.29175686097266745 40-41 0.28481219619115083 42-43 0.3388802157022594 44-45 0.2863662470513504 46-47 0.31195952215526135 48-49 0.31155482141041774 50-51 0.3122832827511362 52-53 0.31566658097802897 54-55 0.29413650135234803 56-57 0.30601851522095685 58-59 0.3065365321743568 60-61 0.2845046236250697 62-63 0.28197929097724544 64-65 0.2908017672148365 66-67 0.2906722629764866 68-69 0.28686807597495645 70-71 0.26064346770908947 72-73 0.25128678648830477 74-75 0.26054633953032696 76-77 0.2563860158733345 78-79 0.2534074183912854 80-81 0.2625050911353701 82-83 0.3052576778206509 84-85 0.31001695858001194 86-87 0.2999641920780962 88-89 0.30689266882981914 90-91 0.28322576927136384 92-93 0.2673615000734937 94-95 0.25271133311015437 96-97 0.25199905979922954 98-99 0.27407953243789784 100-101 0.2747108655998539 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3088702.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.18852857217759 #Duplication Level Percentage of deduplicated Percentage of total 1 94.3758600263677 75.67861348248219 2 3.6264761150244054 5.816035671319082 3 0.7221932097378636 1.7373483250109196 4 0.35903134234412304 1.1516078021547593 5 0.2417127278938456 0.9691293993487312 6 0.09957113653599785 0.4790677756248636 7 0.07231250814757406 0.4059043538002297 8 0.0633575884675571 0.4064441434475976 9 0.0666459382715718 0.4809815752778566 >10 0.3021948712315684 4.626439123430134 >50 0.03568599462363478 1.9370837877909268 >100 0.0332265728730082 5.0753586331829625 >500 0.0012251192529134421 0.6712442791369867 >1k 5.06849228225946E-4 0.5647416479928032 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 3244 0.10502793730181804 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011979142047371355 0.0 0.0 2 0.001295042383499606 0.0 0.0 3 0.0014245466218495665 0.0 0.0 4 0.001521674800612037 0.0 0.0 5 0.0016835550985494878 0.0 0.0 6 0.001974939634836899 0.0 0.0 7 0.0025253326478242317 0.0 0.0 8 0.002816717184111643 0.0 0.0 9 0.0033023580779239954 0.0 0.0 10-11 0.004807844848742287 0.0 0.0 12-13 0.006021947083273168 0.0 0.0 14-15 0.007397929615741499 0.0 4.8564089381235226E-5 16-17 0.008709160029034851 0.0 9.712817876247045E-5 18-19 0.010311774978615613 0.0 1.942563575249409E-4 20-21 0.011623005391908962 0.0 2.1044438731868597E-4 22-23 0.013355124579839686 0.0 2.2663241711243104E-4 24-25 0.01531387618488284 0.0 2.2663241711243104E-4 26-27 0.017175499611163526 0.0 2.9138453628741134E-4 28-29 0.019765584378162736 0.0 2.9138453628741134E-4 30-31 0.034270059073358325 0.0 3.237605958749015E-4 32-33 0.03783142562798224 0.0 3.237605958749015E-4 34-35 0.040146313888487786 0.0 3.237605958749015E-4 36-37 0.04257451835754955 0.0 3.237605958749015E-4 38-39 0.04539123554166119 0.0 3.237605958749015E-4 40-41 0.048240328785360324 0.0 3.237605958749015E-4 42-43 0.05222258411462161 0.0 3.237605958749015E-4 44-45 0.0565286000397578 0.0 3.237605958749015E-4 46-47 0.06123931670973762 0.0 3.237605958749015E-4 48-49 0.06679181092899218 0.0 3.237605958749015E-4 50-51 0.07289469816123408 0.0 3.237605958749015E-4 52-53 0.07975842279378198 0.0 3.237605958749015E-4 54-55 0.08828951449508564 0.0 3.237605958749015E-4 56-57 0.0974357513285516 0.0 3.237605958749015E-4 58-59 0.10774752630716722 0.0 3.237605958749015E-4 60-61 0.11906295913299503 0.0 3.237605958749015E-4 62-63 0.13181912661046613 0.0 3.399486256686465E-4 64-65 0.146242661156693 0.0 3.561366554623916E-4 66-67 0.162624947307963 0.0 3.561366554623916E-4 68-69 0.18205058306045713 0.0 3.561366554623916E-4 70-71 0.20346734647758186 0.0 3.561366554623916E-4 72-73 0.2261144001590312 0.0 3.561366554623916E-4 74-75 0.25305128173582303 0.0 3.561366554623916E-4 76-77 0.28322576927136384 0.0 3.723246852561367E-4 78-79 0.31689687124235355 0.0 3.885127150498818E-4 80-81 0.3550844335257982 0.0 4.047007448436269E-4 82-83 0.3973513793172666 1.6188029793745075E-5 4.532648342248621E-4 84-85 0.44489562282149586 3.237605958749015E-5 4.532648342248621E-4 86-87 0.49606598499952403 3.237605958749015E-5 4.856408938123522E-4 88-89 0.555573182521331 3.237605958749015E-5 5.180169533998424E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1550 0.0 82.62895 2 GAGCGGC 1180 0.0 70.336174 9 GATCGGG 2030 0.0 64.266716 1 AAGCGGC 465 0.0 61.19571 9 TCGGGAG 1765 0.0 59.92541 3 TTGTGTT 29645 0.0 58.234467 1 AGAGCGG 1515 0.0 58.223034 8 GATCCGG 400 0.0 58.114643 1 CGGGAGA 1855 0.0 57.529354 4 GAGAGCG 1835 0.0 57.115074 7 GTTCGGA 280 0.0 52.517475 2 TCGGAGA 450 0.0 49.537743 4 GAGCGTC 3595 0.0 49.4715 9 AAAGCGG 635 0.0 48.543842 8 AGAGCGT 3910 0.0 46.57458 8 TCGGGAA 655 0.0 45.619457 3 TCGGAAA 665 0.0 44.220222 4 GCGTCGG 470 0.0 43.8949 10-11 CGCCGGA 585 0.0 43.8478 58-59 AGCGGCG 1190 0.0 43.64051 10-11 >>END_MODULE