FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_50331580-1.342000000357a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_50331580-1.342000000357a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088702
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT32440.10502793730181804Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA15500.082.628952
GAGCGGC11800.070.3361749
GATCGGG20300.064.2667161
AAGCGGC4650.061.195719
TCGGGAG17650.059.925413
TTGTGTT296450.058.2344671
AGAGCGG15150.058.2230348
GATCCGG4000.058.1146431
CGGGAGA18550.057.5293544
GAGAGCG18350.057.1150747
GTTCGGA2800.052.5174752
TCGGAGA4500.049.5377434
GAGCGTC35950.049.47159
AAAGCGG6350.048.5438428
AGAGCGT39100.046.574588
TCGGGAA6550.045.6194573
TCGGAAA6650.044.2202224
GCGTCGG4700.043.894910-11
CGCCGGA5850.043.847858-59
AGCGGCG11900.043.6405110-11
Produced by FastQC (version 0.11.2)