##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_50330665-2.34200000035755.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2474467 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98889538635997 33.0 33.0 33.0 33.0 33.0 2 31.946592538918484 33.0 33.0 33.0 33.0 33.0 3 31.8992971011535 33.0 33.0 33.0 33.0 33.0 4 35.65270945217697 37.0 37.0 37.0 33.0 37.0 5 35.65073771442496 37.0 37.0 37.0 33.0 37.0 6 35.71641286790246 37.0 37.0 37.0 33.0 37.0 7 35.613897053385635 37.0 37.0 37.0 33.0 37.0 8 35.57253905588557 37.0 37.0 37.0 33.0 37.0 9 35.52677162394972 37.0 37.0 37.0 33.0 37.0 10-11 35.45505941279475 37.0 37.0 37.0 33.0 37.0 12-13 35.42178921763758 37.0 37.0 37.0 33.0 37.0 14-15 37.09234493731377 40.0 37.0 40.0 33.0 40.0 16-17 37.072844374162194 40.0 37.0 40.0 33.0 40.0 18-19 37.126927738377596 40.0 37.0 40.0 33.0 40.0 20-21 37.12440072953085 40.0 37.0 40.0 33.0 40.0 22-23 37.15087814870839 40.0 37.0 40.0 33.0 40.0 24-25 36.89706328676034 40.0 37.0 40.0 33.0 40.0 26-27 36.82350118227481 40.0 37.0 40.0 33.0 40.0 28-29 36.73270041588754 40.0 37.0 40.0 33.0 40.0 30-31 36.792620592636716 40.0 37.0 40.0 33.0 40.0 32-33 36.70463275525598 40.0 37.0 40.0 33.0 40.0 34-35 36.634259620354605 40.0 37.0 40.0 33.0 40.0 36-37 36.56857981941161 40.0 37.0 40.0 33.0 40.0 38-39 36.52075335819795 40.0 37.0 40.0 33.0 40.0 40-41 36.44725389346473 40.0 37.0 40.0 33.0 40.0 42-43 36.3046692479633 38.5 37.0 40.0 33.0 40.0 44-45 36.219567486654704 37.0 37.0 40.0 33.0 40.0 46-47 36.11344523891408 37.0 37.0 40.0 33.0 40.0 48-49 36.00125340123752 37.0 37.0 40.0 30.0 40.0 50-51 34.9023569520224 37.0 35.0 38.5 27.0 38.5 52-53 35.331666172957654 37.0 37.0 38.5 30.0 40.0 54-55 36.07695131921339 37.0 37.0 40.0 33.0 40.0 56-57 36.14543677486909 37.0 37.0 40.0 30.0 40.0 58-59 35.950836685233625 37.0 37.0 40.0 27.0 40.0 60-61 35.76018047522962 37.0 37.0 40.0 27.0 40.0 62-63 35.571905182004855 37.0 37.0 40.0 27.0 40.0 64-65 35.33823243551035 37.0 37.0 40.0 27.0 40.0 66-67 35.091231768295955 37.0 35.0 40.0 27.0 40.0 68-69 34.88007821482364 37.0 33.0 38.5 27.0 40.0 70-71 34.61609934583892 37.0 33.0 37.0 27.0 40.0 72-73 34.391134737298984 37.0 33.0 37.0 27.0 40.0 74-75 34.168912537528286 37.0 33.0 37.0 27.0 40.0 76-77 33.923454626794374 37.0 33.0 37.0 27.0 37.0 78-79 33.67766816045637 37.0 33.0 37.0 27.0 37.0 80-81 33.4251804530026 37.0 33.0 37.0 27.0 37.0 82-83 33.211552427249984 37.0 33.0 37.0 27.0 37.0 84-85 33.01517599547701 37.0 33.0 37.0 27.0 37.0 86-87 32.85839900067368 37.0 33.0 37.0 27.0 37.0 88-89 32.71383958646447 37.0 33.0 37.0 27.0 37.0 90-91 32.56347750848971 35.0 33.0 37.0 27.0 37.0 92-93 32.40836289188742 33.0 33.0 37.0 22.0 37.0 94-95 32.26005277096037 33.0 33.0 37.0 22.0 37.0 96-97 32.131266854639804 33.0 33.0 37.0 22.0 37.0 98-99 31.975194456018205 33.0 33.0 37.0 22.0 37.0 100-101 30.741237203809952 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.39662514580709285 2306 2 0.3000486577394881 2306 3 0.37959578751573986 2306 4 0.40389507477291176 2306 5 0.3238244053050252 2306 6 0.4027327070389717 2306 7 0.44137428733544937 2306 8 0.3041298462582205 2306 9 0.3597813101017522 2306 10-11 0.38319384382445776 2306 12-13 0.46506274716607976 2306 14-15 0.8186196104300478 2306 16-17 0.6782305599185747 2306 18-19 0.6522163159109269 2306 20-21 0.7088195477723218 2306 22-23 0.7686221031864235 2306 24-25 0.977011114350276 2306 26-27 0.8996795032891782 2306 28-29 0.8532738366326598 2306 30-31 0.8584443286242518 2306 32-33 0.9136820141512132 2306 34-35 0.9405049948241668 2306 36-37 0.7870100858856617 2306 38-39 0.8309103048902955 2306 40-41 0.7567046460448594 2306 42-43 0.7590474132819196 2306 44-45 0.7188402394734794 2306 46-47 0.6309696310682256 2306 48-49 0.7653289664127385 2306 50-51 0.8928990279764903 2306 52-53 0.8428083277607641 2306 54-55 1.043450339847972 2306 56-57 0.9448107540356219 2306 58-59 1.0414024368916088 2306 60-61 0.9582468836721532 2306 62-63 0.9271647708353186 2306 64-65 1.067843745094713 2306 66-67 0.8265199395404963 2306 68-69 0.8624422451143019 2306 70-71 0.812326203815644 2306 72-73 0.8728966254041239 2306 74-75 0.9401850810576065 2306 76-77 0.7662797401624459 2306 78-79 0.7717950414245891 2306 80-81 0.8255002996240677 2306 82-83 0.6381932912950035 2306 84-85 0.7356323778332481 2306 86-87 0.7254161408326212 2306 88-89 0.7680664591821085 2306 90-91 0.947658179275372 2306 92-93 0.8143121910287832 2306 94-95 0.754577600937381 2306 96-97 0.7918389519280851 2306 98-99 0.8602460416991988 2306 100-101 0.8317391303837063 2307 1 0.25789100776933793 2307 2 0.23246752150835448 2307 3 0.25805878766689005 2307 4 0.3894564604768149 2307 5 0.3259693240788195 2307 6 0.32761736367201877 2307 7 0.32650417008468935 2307 8 0.23293185873905742 2307 9 0.30059630725865816 2307 10-11 0.27320107752703393 2307 12-13 0.20872785912651892 2307 14-15 0.4375699318079711 2307 16-17 0.4047750094014191 2307 18-19 0.5131762750279094 2307 20-21 0.46830883397502276 2307 22-23 0.4461105328611623 2307 24-25 0.4940696965445781 2307 26-27 0.46547380701831287 2307 28-29 0.3233232489393103 2307 30-31 0.483783520896786 2307 32-33 0.32435298505085797 2307 34-35 0.24576364467697687 2307 36-37 0.2590091789736988 2307 38-39 0.3322904665509583 2307 40-41 0.3014762265908786 2307 42-43 0.2607730652148703 2307 44-45 0.23150191812433718 2307 46-47 0.001550234668521 2307 48-49 0.1347048526312733 2307 50-51 0.04522784834313853 2307 52-53 0.2683677700818876 2307 54-55 0.14992882228195015 2307 56-57 0.20819670265833423 2307 58-59 0.11040872049594697 2307 60-61 0.027245969562471828 2307 62-63 0.07393335713231153 2307 64-65 0.2352985614450418 2307 66-67 0.3566342752274778 2307 68-69 0.3072042886892632 2307 70-71 0.09195289437872134 2307 72-73 0.04008962490748047 2307 74-75 0.2002681786658087 2307 76-77 0.22598333829968453 2307 78-79 0.1524767369181106 2307 80-81 0.10450978621113194 2307 82-83 0.08898432006426304 2307 84-85 0.29518716323340755 2307 86-87 0.006747789900870771 2307 88-89 0.13827098222558476 2307 90-91 0.1226193116197436 2307 92-93 0.19864582666610175 2307 94-95 0.14159238504194605 2307 96-97 0.345777720975029 2307 98-99 0.15107057582715555 2307 100-101 0.3189523914519903 2201 1 0.20801048208235784 2201 2 0.23228692080262903 2201 3 0.2391860139178945 2201 4 0.34625121472092957 2201 5 0.3596999789647626 2201 6 0.35608281336777736 2201 7 0.3717863239564849 2201 8 0.45909951176342645 2201 9 0.3265889165220841 2201 10-11 0.26991206082841757 2201 12-13 0.3704384256570421 2201 14-15 0.6567914038426466 2201 16-17 0.3908757012410433 2201 18-19 0.5122836907707295 2201 20-21 0.45607313616168454 2201 22-23 0.5914107468035397 2201 24-25 0.6875034716550132 2201 26-27 0.5667525989385211 2201 28-29 0.4576137660130257 2201 30-31 0.61079096720281 2201 32-33 0.3324557051753345 2201 34-35 0.42446456990827386 2201 36-37 0.4181427123570387 2201 38-39 0.3844736512360214 2201 40-41 0.36387377042127866 2201 42-43 0.44456206802112064 2201 44-45 0.5863510470732365 2201 46-47 0.48190296173918057 2201 48-49 0.48131581092640374 2201 50-51 0.5203396818570738 2201 52-53 0.6340286297412447 2201 54-55 0.8091005596607417 2201 56-57 0.7858550061230929 2201 58-59 0.7220303759097959 2201 60-61 0.6664891136818341 2201 62-63 0.5723531009571516 2201 64-65 0.5501272686218286 2201 66-67 0.6157962879528824 2201 68-69 0.6798599526052413 2201 70-71 0.6982398827924925 2201 72-73 0.7571820924688168 2201 74-75 0.711903031823546 2201 76-77 0.7399625275414365 2201 78-79 0.6559810852274026 2201 80-81 0.6499412829742042 2201 82-83 0.708385698186575 2201 84-85 0.6006072682596653 2201 86-87 0.6017993305638214 2201 88-89 0.6107189553299719 2201 90-91 0.7037645979413227 2201 92-93 0.7298307062193246 2201 94-95 0.7451078472408312 2201 96-97 0.7034990957631706 2201 98-99 0.5397441331053585 2201 100-101 0.3776997171723089 1101 1 0.35132250905114404 1101 2 0.3246139402177093 1101 3 0.3885319307134836 1101 4 0.39506356474772275 1101 5 0.3293123564229177 1101 6 0.20985654098651452 1101 7 0.31694075644005437 1101 8 0.2724244274355385 1101 9 0.4162726423994627 1101 10-11 0.3934330031223894 1101 12-13 0.379713821008103 1101 14-15 0.6305747929463479 1101 16-17 0.6069404916836447 1101 18-19 0.39330388172793107 1101 20-21 0.48734168846514336 1101 22-23 0.5200011448876438 1101 24-25 0.6716744698538264 1101 26-27 0.650346294836389 1101 28-29 0.5685119143292567 1101 30-31 0.5322100490726314 1101 32-33 0.5037870470460035 1101 34-35 0.4980471655196652 1101 36-37 0.5240479168517496 1101 38-39 0.5831489453331002 1101 40-41 0.5110181191921512 1101 42-43 0.43163924358650974 1101 44-45 0.31345292430582106 1101 46-47 0.4158555848289822 1101 48-49 0.5301498438265497 1101 50-51 0.29612431612919465 1101 52-53 0.5316308401168826 1101 54-55 0.6840971139328076 1101 56-57 0.6303814084671302 1101 58-59 0.6418587080631752 1101 60-61 0.6272486596928957 1101 62-63 0.6559836073144396 1101 64-65 0.6365693208906649 1101 66-67 0.45615201481994205 1101 68-69 0.4281838160696978 1101 70-71 0.5213938634654838 1101 72-73 0.4821916564648916 1101 74-75 0.6716448274612858 1101 76-77 0.4643705178651558 1101 78-79 0.47929104812111945 1101 80-81 0.38714452175676684 1101 82-83 0.443358290362319 1101 84-85 0.545884234190325 1101 86-87 0.5017997675829662 1101 88-89 0.5161552382736474 1101 90-91 0.5005557343861042 1101 92-93 0.45713882287520136 1101 94-95 0.48515893814148114 1101 96-97 0.5053209949946265 1101 98-99 0.5445326864307773 1101 100-101 0.6060291139715837 2304 1 0.22923139577638807 2304 2 0.19979180407811725 2304 3 0.16899011397289598 2304 4 0.3363139861457043 2304 5 0.22913162258996067 2304 6 0.20403752691389343 2304 7 0.17267195100698984 2304 8 0.20245730888466085 2304 9 0.164445205991278 2304 10-11 0.26418962051815953 2304 12-13 0.19667441587152723 2304 14-15 0.3549338627377949 2304 16-17 0.304033995223719 2304 18-19 0.25954810828604735 2304 20-21 0.2634208064125758 2304 22-23 0.2577739866434783 2304 24-25 0.4440461126062587 2304 26-27 0.3364611195431024 2304 28-29 0.3521776847704885 2304 30-31 0.25964553093192677 2304 32-33 0.3022268075629242 2304 34-35 0.3158472480293071 2304 36-37 0.22374093731010447 2304 38-39 0.19680415890702818 2304 40-41 0.3174210196674565 2304 42-43 0.10950773565496519 2304 44-45 0.2334040140186744 2304 46-47 0.2400812629282285 2304 48-49 0.20528747844645778 2304 50-51 -0.03645802727037761 2304 52-53 0.14362924418056622 2304 54-55 0.5122874895501823 2304 56-57 0.4439403828464634 2304 58-59 0.3458806726991597 2304 60-61 0.3558796050436541 2304 62-63 0.4612277667135416 2304 64-65 0.4239017087045127 2304 66-67 0.3813563949108385 2304 68-69 0.3703408841255964 2304 70-71 0.4596657992363191 2304 72-73 0.44753975684081837 2304 74-75 0.5270971301717609 2304 76-77 0.41214776961915334 2304 78-79 0.4768122850908654 2304 80-81 0.4554541691322598 2304 82-83 0.44750974286617407 2304 84-85 0.5601424562894763 2304 86-87 0.446089216968403 2304 88-89 0.42477994412214315 2304 90-91 0.35001632842244845 2304 92-93 0.40264176612716085 2304 94-95 0.3372690048141749 2304 96-97 0.4005368913672811 2304 98-99 0.3285448385727392 2304 100-101 0.3794349393376386 2202 1 0.18086529209733015 2202 2 0.14401418546692213 2202 3 0.17395251728561334 2202 4 0.25974972520493367 2202 5 0.2204211029624048 2202 6 0.2168221976316005 2202 7 0.2522937506676044 2202 8 0.08135721107422711 2202 9 0.1603787565099779 2202 10-11 0.19453498633175315 2202 12-13 0.2468542484385452 2202 14-15 0.23753438798752313 2202 16-17 0.2794632957379264 2202 18-19 0.15989185676176731 2202 20-21 0.23996940741532313 2202 22-23 0.22254554408849714 2202 24-25 0.29704550417915954 2202 26-27 0.3282422192814991 2202 28-29 0.3935815340346025 2202 30-31 0.34136986981298634 2202 32-33 0.29023796976137106 2202 34-35 0.30727279015839315 2202 36-37 0.21207299987797512 2202 38-39 0.3669724904048124 2202 40-41 0.296965546519111 2202 42-43 0.16405438044905907 2202 44-45 0.2897951941362038 2202 46-47 0.2846581750451662 2202 48-49 0.26102787261479676 2202 50-51 0.34269859521472057 2202 52-53 0.3245862460982707 2202 54-55 0.37140067496127926 2202 56-57 0.39281581959186695 2202 58-59 0.37386318491262926 2202 60-61 0.4565730065618965 2202 62-63 0.4677856866630137 2202 64-65 0.408041528846276 2202 66-67 0.5053908056992498 2202 68-69 0.48737196525178206 2202 70-71 0.5715984152931526 2202 72-73 0.5034752584337667 2202 74-75 0.30178527919276377 2202 76-77 0.4443366438749834 2202 78-79 0.4553594535943617 2202 80-81 0.386740414828985 2202 82-83 0.4839396364042656 2202 84-85 0.35481756391946107 2202 86-87 0.4026072169119246 2202 88-89 0.45874789002604643 2202 90-91 0.4017737072055425 2202 92-93 0.38888284188788447 2202 94-95 0.32046666147131475 2202 96-97 0.4040385561983939 2202 98-99 0.30858611919625645 2202 100-101 0.3091115487813525 1102 1 0.18736471831013546 1102 2 0.14218763501264675 1102 3 0.267792338794429 1102 4 0.11862494500376641 1102 5 0.182536947899834 1102 6 0.168094742509453 1102 7 0.19965213402299042 1102 8 0.14094172928413684 1102 9 0.1471380680177674 1102 10-11 0.20049175600485114 1102 12-13 0.20252604696704424 1102 14-15 0.13668962205620971 1102 16-17 0.27840376102876974 1102 18-19 0.3640510875701537 1102 20-21 0.27676147711479615 1102 22-23 0.23699377445799286 1102 24-25 0.21176399236075838 1102 26-27 0.21445856840452393 1102 28-29 0.1804999286713027 1102 30-31 0.34579992643146085 1102 32-33 0.1772222084725783 1102 34-35 0.28274226780340683 1102 36-37 0.2677091674557346 1102 38-39 0.15914945411039128 1102 40-41 0.2179131157171028 1102 42-43 0.18972526799615963 1102 44-45 0.08136483691571073 1102 46-47 0.18444312226833404 1102 48-49 0.17964599467800468 1102 50-51 0.09202480760097131 1102 52-53 0.22241662485898672 1102 54-55 0.410667577434495 1102 56-57 0.35312641399957556 1102 58-59 0.43093922739101487 1102 60-61 0.46065614748591344 1102 62-63 0.34354836050044213 1102 64-65 0.27609832689743286 1102 66-67 0.33476846996482124 1102 68-69 0.3366030161288833 1102 70-71 0.22588775040999565 1102 72-73 0.29426445013112357 1102 74-75 0.25650928569327647 1102 76-77 0.44416220295430975 1102 78-79 0.38527265257869914 1102 80-81 0.2523400754283429 1102 82-83 0.29885629034559713 1102 84-85 0.3819559616539365 1102 86-87 0.35613908301906605 1102 88-89 0.3232042362129519 1102 90-91 0.424594118693868 1102 92-93 0.35205680153949714 1102 94-95 0.3507421557879553 1102 96-97 0.32333543585775004 1102 98-99 0.40660007053762115 1102 100-101 0.4005378157609414 2305 1 0.08649747535994123 2305 2 0.04191538283073726 2305 3 0.02406439644156677 2305 4 0.08459120287079713 2305 5 0.09130014099508088 2305 6 0.16509469985847858 2305 7 0.041166895989050545 2305 8 0.14482624896756136 2305 9 0.15922450552839962 2305 10-11 0.08891603016146377 2305 12-13 0.039473198161665835 2305 14-15 0.00831896461554038 2305 16-17 0.0917219818925048 2305 18-19 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-0.9409095580081512 1213 58-59 -1.3390195975779235 1213 60-61 -1.0033800344189814 1213 62-63 -0.9708196712622126 1213 64-65 -0.8438627001719041 1213 66-67 -0.7263293213709474 1213 68-69 -0.7458121990624207 1213 70-71 -0.7848900447985443 1213 72-73 -0.6810763931532193 1213 74-75 -0.7484381605436212 1213 76-77 -0.9481018243949251 1213 78-79 -0.7994128371326426 1213 80-81 -0.7212833721970426 1213 82-83 -0.6622316855674271 1213 84-85 -0.639998979367995 1213 86-87 -0.790500178113767 1213 88-89 -0.6934264749465697 1213 90-91 -0.649309523458875 1213 92-93 -0.5818958376505421 1213 94-95 -0.5525896403215782 1213 96-97 -0.6398490245688038 1213 98-99 -0.7860365788920021 1213 100-101 -1.1899027511264322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1210.0 3 79.0 4 106.0 5 147.0 6 310.0 7 1436.0 8 5676.0 9 9127.0 10 10874.0 11 9650.0 12 7705.0 13 6135.0 14 5300.0 15 4903.0 16 4925.0 17 5259.0 18 5632.0 19 6352.0 20 7291.0 21 8257.0 22 9325.0 23 10536.0 24 11895.0 25 13797.0 26 16389.0 27 19605.0 28 23584.0 29 29266.0 30 36623.0 31 47832.0 32 65455.0 33 94618.0 34 144488.0 35 242941.0 36 473441.0 37 830143.0 38 303415.0 39 740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.92919042793973 24.788815297030926 26.199776458153103 13.082217816876234 2 31.58099477249378 22.311258851826874 35.12658304707671 10.981163328602644 3 39.878764580319476 18.427783791439467 26.855992473368474 14.837459154872585 4 30.69486211685188 17.806696534065345 36.87581129155998 14.622630057522798 5 30.521538371262473 16.684128843752973 38.160583232667825 14.633749552316724 6 23.460439519962893 17.39318915273831 44.61690653815594 14.529464789142862 7 33.9691335742994 17.242117479859694 32.59419447677289 16.194554469068017 8 29.974896596150003 28.15987679747898 26.546364012304068 15.318862594066953 9 30.80035087066257 27.696893386622083 24.861686881920207 16.641068860795137 10-11 24.7259442417848 28.862601346116733 30.5338994947511 15.877554917347364 12-13 25.85521257395372 26.364855896468836 29.824979564087826 17.954951965489613 14-15 23.780731707662508 27.276902567186234 31.173377483792088 17.768988241359168 16-17 21.97563053448025 28.840768207252736 32.533817089214644 16.64978416905237 18-19 21.07732743364264 29.34647681487913 32.34430165459458 17.231894096883646 20-21 21.089162928188653 29.556069685486396 31.807733566964185 17.54703381936076 22-23 21.235235185011344 29.037179495479513 31.984073098175298 17.743512221333845 24-25 20.779001259104664 29.68995181717922 31.533114655633355 17.99793226808276 26-27 20.518505576684376 29.97035118883058 31.183990452709864 18.327152781775183 28-29 20.266932266875468 29.76577753480678 30.909352771914804 19.057937426402948 30-31 20.196491472503386 30.061243095925377 30.649109583465133 19.093155848106104 32-33 19.555406360037463 30.107252624185517 31.940662502382956 18.39667851339407 34-35 19.87353567436328 30.12390124290264 31.668923462702487 18.333639620031597 36-37 20.072265903189056 30.620032453606516 31.555043995120414 17.752657648084018 38-39 20.772077821806494 30.072945278071955 30.766144279907543 18.388832620214004 40-41 20.25395765265058 29.648980465232395 30.682861749026745 19.414200133090283 42-43 20.40747269813304 30.186898550489573 31.225286906558836 18.18034184481855 44-45 19.89986036216676 30.490025246666146 31.428421476464912 18.18169291470218 46-47 20.287490947373456 30.12262115824569 31.13890706902454 18.450980825356314 48-49 20.096743299872813 30.485733251582236 30.52771007413338 18.889813374411567 50-51 19.940948409008175 30.137188435665912 30.409583002611452 19.51228015271446 52-53 20.48076794442546 29.915199852761194 30.252892379053982 19.351139823759365 54-55 20.907204018057264 29.936909821494435 30.0281242932449 19.127761867203404 56-57 20.96347883118802 30.285899707191 29.803201624343096 18.947419837277888 58-59 20.74525460190217 30.20862848296837 30.387309285199777 18.658807629929683 60-61 20.170257207491627 30.613713788276563 30.038070891127948 19.17795811310386 62-63 20.529717723118736 30.48526286802811 29.95107108866068 19.033948320192483 64-65 20.112168266990953 30.35548659308343 30.28407436355674 19.248270776368877 66-67 20.447137831528437 29.56311604305817 30.218289994163804 19.771456131249597 68-69 20.31471321675547 29.674263127370644 30.96392954424883 19.047094111625054 70-71 19.659021776278724 30.78858406073882 30.610489833070343 18.941904329912113 72-73 19.51131173323416 31.380468188510253 30.073730250753343 19.034489827502245 74-75 20.039561108318793 30.634900279792333 29.928946807042962 19.39659180484591 76-77 20.05697306197593 30.403811061087826 30.133855498617606 19.405360378318633 78-79 19.833461999934773 30.590995984832265 30.449033947117925 19.12650806811504 80-81 19.17088358075669 30.896234537812845 30.308804122466558 19.624077758963907 82-83 18.678073210228597 30.731534796240783 30.26059939075924 20.329792602771377 84-85 18.692301783246954 30.429046610156607 30.784759593761585 20.093892012834853 86-87 18.8096482557898 30.334181218218248 31.45386755688162 19.402302969110334 88-89 19.005357432761514 30.79845874241699 30.875395960147493 19.320787864674003 90-91 19.231013169298155 30.54745869282897 30.747416585765585 19.47411155210729 92-93 18.665678253174324 31.57956796146403 30.57921462862774 19.175539156733908 94-95 18.439289192688708 31.82802115795052 30.53176042380723 19.20092922555354 96-97 19.034156973799888 31.790293763749283 30.29842280750797 18.877126454942854 98-99 19.305552431958432 30.90531570044184 30.015100650403838 19.774031217195898 100-101 19.170901941269957 30.944708696043303 29.560298666891434 20.32409069579531 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1021.0 1 626.0 2 196.0 3 198.5 4 232.0 5 260.0 6 334.0 7 468.0 8 649.5 9 818.5 10 987.5 11 1076.0 12 1082.0 13 1039.0 14 961.0 15 912.5 16 911.0 17 952.5 18 1057.0 19 1288.0 20 1715.0 21 2337.0 22 3244.0 23 4512.0 24 6415.5 25 9092.0 26 12776.5 27 18096.0 28 23776.0 29 29388.5 30 35801.0 31 42564.0 32 51153.0 33 63279.0 34 78701.5 35 97687.5 36 124346.0 37 153463.5 38 178335.5 39 189560.5 40 181586.0 41 162743.5 42 144744.5 43 134955.5 44 121734.5 45 106261.0 46 99353.0 47 88347.0 48 68911.5 49 51920.5 50 39475.5 51 31478.0 52 25908.5 53 20337.0 54 15122.5 55 10913.5 56 7886.0 57 5688.5 58 4203.0 59 3168.0 60 2253.5 61 1635.0 62 1212.0 63 904.5 64 660.5 65 475.5 66 374.0 67 284.5 68 211.5 69 176.5 70 146.5 71 112.0 72 94.5 73 74.5 74 53.5 75 44.0 76 40.0 77 30.0 78 26.5 79 28.0 80 19.5 81 9.0 82 9.5 83 10.5 84 5.5 85 4.0 86 6.5 87 6.0 88 4.5 89 4.0 90 3.5 91 1.5 92 0.5 93 0.5 94 1.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17118838117461255 2 0.16460110399532504 3 0.13101811420398818 4 0.1252390918933249 5 0.13788828058729416 6 0.15793300132917512 7 0.13348329155329208 8 0.12483496445901279 9 0.117237368693945 10-11 0.10571973681604968 12-13 0.13445319739564116 14-15 0.13014924022021712 16-17 0.12012687984927664 18-19 0.11820727453629408 20-21 0.11159979098529096 22-23 0.12329928020862674 24-25 0.4208785164643537 26-27 0.3066923098994652 28-29 0.38834625800222833 30-31 0.22041110267382835 32-33 0.25926795548293835 34-35 0.2722000333809261 36-37 0.2681991717812361 38-39 0.28846616261198876 40-41 0.281636408972114 42-43 0.335001436673029 44-45 0.2833943633113717 46-47 0.3088139789296038 48-49 0.30832902600842926 50-51 0.30879377255788826 52-53 0.31222885574954123 54-55 0.29117381642187995 56-57 0.303115782105803 58-59 0.30301475024722496 60-61 0.28147475799838917 62-63 0.2791510252510945 64-65 0.28703151022018075 66-67 0.2872133675656212 68-69 0.2847481902163173 70-71 0.2582980496405893 72-73 0.24833630838479562 74-75 0.2580959859234332 76-77 0.2539132669783028 78-79 0.25061962838865903 80-81 0.25985394026269093 82-83 0.3015598914837013 84-85 0.30675292901461204 86-87 0.2969326323608276 88-89 0.3039240369744272 90-91 0.28076753498834295 92-93 0.264258929296693 94-95 0.24954869068773197 96-97 0.24882126130597013 98-99 0.27046228541338396 100-101 0.27080579373254926 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2474467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.41590267163004 #Duplication Level Percentage of deduplicated Percentage of total 1 91.97218731013037 56.485549043358944 2 3.8861244613356423 4.773396833744611 3 1.2535725638091766 2.3096787171219035 4 0.686267425406783 1.6859093362197244 5 0.38688581328928745 1.1880470727004653 6 0.2659790817966734 0.9801207240188409 7 0.22022812480638823 0.946785635506531 8 0.1477588171389434 0.7259792905824423 9 0.08488571915415484 0.4691999759204606 >10 0.8972244344609361 10.682107146168402 >50 0.09468711821449148 4.043283867714539 >100 0.09771553012648733 11.878341544005789 >500 0.005274377155887191 2.1708843769140547 >1k 0.0010760132762823505 1.0337906067709184 >5k 1.3320989873833068E-4 0.6269258292523963 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7717 0.3118651410586603 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7609 0.30750056476808946 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3882 0.15688226999996363 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.4247646058726992E-4 0.0 0.0 2 2.4247646058726992E-4 0.0 0.0 3 2.8288920401848156E-4 0.0 0.0 4 3.233019474496932E-4 0.0 0.0 5 3.6371469088090485E-4 0.0 0.0 6 4.041274343121165E-4 0.0 0.0 7 5.253656646057515E-4 0.0 0.0 8 6.87016638330598E-4 0.0 0.0 9 8.486676120554446E-4 0.0 0.0 10-11 0.0013336205332299845 0.0 0.0 12-13 0.0020408435432761884 0.0 0.0 14-15 0.0028490984119004213 0.0 0.0 16-17 0.003697766023955866 0.0 0.0 18-19 0.0048091164683141865 0.0 6.061911514681748E-5 20-21 0.006142737001544171 0.0 8.08254868624233E-5 22-23 0.0075167702782053675 0.0 8.08254868624233E-5 24-25 0.009496994706334739 0.0 8.08254868624233E-5 26-27 0.012042997542501072 0.0 8.08254868624233E-5 28-29 0.014791064095823463 0.0 8.08254868624233E-5 30-31 0.022004738798294746 0.0 1.2123823029363496E-4 32-33 0.024853837210195164 0.0 1.2123823029363496E-4 34-35 0.028006031197829673 0.0 1.2123823029363496E-4 36-37 0.03127946341575782 0.0 1.2123823029363496E-4 38-39 0.03542176961745701 0.0 1.2123823029363496E-4 40-41 0.03948325033229378 0.0 1.2123823029363496E-4 42-43 0.043564937418846164 0.0 1.2123823029363496E-4 44-45 0.048677149462894434 0.0 1.2123823029363496E-4 46-47 0.05382977425037392 0.0 1.2123823029363496E-4 48-49 0.058881367179275376 0.0 1.2123823029363496E-4 50-51 0.06429667479905773 0.0 1.2123823029363496E-4 52-53 0.0700352843662898 0.0 1.2123823029363496E-4 54-55 0.07627905322641199 0.0 1.2123823029363496E-4 56-57 0.08389685536319538 0.0 1.2123823029363496E-4 58-59 0.09195919767772212 0.0 1.2123823029363496E-4 60-61 0.10076917574572625 0.0 1.2123823029363496E-4 62-63 0.1099226621328957 0.0 1.616509737248466E-4 64-65 0.12012687984927664 0.0 1.616509737248466E-4 66-67 0.13178595632918122 0.0 1.616509737248466E-4 68-69 0.1432631754636453 0.0 1.616509737248466E-4 70-71 0.15797341407260634 0.0 1.616509737248466E-4 72-73 0.17189560418465877 0.0 1.8185734544045242E-4 74-75 0.19018237058728202 0.0 2.0206371715605825E-4 76-77 0.20871161345049255 0.0 2.0206371715605825E-4 78-79 0.23144378163054913 0.0 2.0206371715605825E-4 80-81 0.2575100011436806 0.0 2.0206371715605825E-4 82-83 0.2872537803090524 0.0 2.0206371715605825E-4 84-85 0.32204915240332566 0.0 2.0206371715605825E-4 86-87 0.3604210522912611 0.0 2.0206371715605825E-4 88-89 0.4033393858152079 0.0 2.0206371715605825E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 515 0.0 58.006714 9 CGGGAGA 740 0.0 54.473457 4 AGAGCGG 655 0.0 49.22406 8 ACCGCCG 50 2.8538296E-5 47.42683 2 TTGTGTT 33525 0.0 45.445652 1 TCGGGAG 910 0.0 45.33938 3 GTTCGGA 105 2.1827873E-11 45.168407 2 ATTCGGA 160 0.0 44.462654 2 GAGAGCG 875 0.0 42.26651 7 GACGCGA 45 9.7131147E-4 42.146088 8 AGAGCGT 1860 0.0 40.021793 8 GGCGCCG 480 0.0 39.58486 56-57 GCGTCGG 210 0.0 39.51516 10-11 AGCGGCG 420 0.0 38.386154 10-11 GAGCGTC 1930 0.0 38.081966 9 CGGAAAG 345 0.0 37.114395 5 AAGAGCG 1950 0.0 36.958878 7 GGGCGCC 635 0.0 35.90726 54-55 GATCGGG 1360 0.0 35.57516 1 TGTGTTT 55460 0.0 35.468254 1 >>END_MODULE