FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474467
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG77170.3118651410586603No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC76090.30750056476808946No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA38820.15688226999996363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC5150.058.0067149
CGGGAGA7400.054.4734574
AGAGCGG6550.049.224068
ACCGCCG502.8538296E-547.426832
TTGTGTT335250.045.4456521
TCGGGAG9100.045.339383
GTTCGGA1052.1827873E-1145.1684072
ATTCGGA1600.044.4626542
GAGAGCG8750.042.266517
GACGCGA459.7131147E-442.1460888
AGAGCGT18600.040.0217938
GGCGCCG4800.039.5848656-57
GCGTCGG2100.039.5151610-11
AGCGGCG4200.038.38615410-11
GAGCGTC19300.038.0819669
CGGAAAG3450.037.1143955
AAGAGCG19500.036.9588787
GGGCGCC6350.035.9072654-55
GATCGGG13600.035.575161
TGTGTTT554600.035.4682541
Produced by FastQC (version 0.11.2)