##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_50330665-1.342000000356cf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2852942 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.302086758160524 33.0 33.0 33.0 22.0 33.0 2 29.41541678730237 33.0 33.0 33.0 15.0 33.0 3 29.14948393623144 33.0 33.0 33.0 15.0 33.0 4 32.183517575891834 37.0 37.0 37.0 15.0 37.0 5 32.33993610805968 37.0 37.0 37.0 15.0 37.0 6 33.49084383769456 37.0 37.0 37.0 22.0 37.0 7 32.30259465492113 37.0 33.0 37.0 15.0 37.0 8 32.99990746394424 37.0 33.0 37.0 15.0 37.0 9 31.87936347812188 37.0 33.0 37.0 6.0 37.0 10-11 32.02007629317385 37.0 33.0 37.0 15.0 37.0 12-13 31.971883585435663 37.0 33.0 37.0 10.5 37.0 14-15 33.27662917788024 37.0 33.0 40.0 6.0 40.0 16-17 33.10006968245411 37.0 33.0 40.0 10.5 40.0 18-19 33.30141727381769 37.0 33.0 40.0 10.5 40.0 20-21 33.276197342953346 37.0 33.0 40.0 10.5 40.0 22-23 34.77388078692101 37.0 33.0 40.0 18.5 40.0 24-25 33.58690239759518 37.0 33.0 40.0 15.0 40.0 26-27 33.41443674634816 37.0 33.0 40.0 10.5 40.0 28-29 32.836665449209974 37.0 33.0 40.0 6.0 40.0 30-31 32.8226425563506 37.0 33.0 40.0 10.5 40.0 32-33 32.53212946495232 37.0 33.0 40.0 6.0 40.0 34-35 33.04484791488926 37.0 33.0 40.0 10.5 40.0 36-37 32.565149589441354 37.0 33.0 40.0 6.0 40.0 38-39 32.488830477450996 37.0 33.0 40.0 6.0 40.0 40-41 32.250590793643894 37.0 33.0 40.0 6.0 40.0 42-43 32.10852639135321 37.0 33.0 40.0 6.0 40.0 44-45 31.93076725709811 37.0 33.0 40.0 6.0 40.0 46-47 31.84766269345819 37.0 33.0 40.0 6.0 40.0 48-49 31.727672697166643 37.0 33.0 40.0 6.0 40.0 50-51 30.953891807124016 35.0 30.0 38.5 10.5 38.5 52-53 31.439549945284554 37.0 33.0 38.5 10.5 40.0 54-55 31.887809846817774 37.0 33.0 40.0 6.0 40.0 56-57 32.40187006956327 37.0 33.0 40.0 10.5 40.0 58-59 31.50118912336809 37.0 33.0 40.0 6.0 40.0 60-61 31.41782097217539 37.0 33.0 40.0 6.0 40.0 62-63 30.897685617162914 37.0 33.0 37.0 6.0 40.0 64-65 30.691324078793052 37.0 27.0 37.0 6.0 40.0 66-67 30.62174940815481 37.0 27.0 37.0 6.0 40.0 68-69 31.01488516065171 37.0 27.0 37.0 10.5 38.5 70-71 30.51464383082446 37.0 27.0 37.0 10.5 37.0 72-73 31.286583638924313 37.0 27.0 37.0 15.0 37.0 74-75 31.657720170967373 35.0 27.0 37.0 22.0 37.0 76-77 31.268390489536763 33.0 27.0 37.0 22.0 37.0 78-79 30.66892299247584 33.0 27.0 37.0 15.0 37.0 80-81 29.95074803483562 33.0 27.0 37.0 15.0 37.0 82-83 29.353664217498988 33.0 27.0 37.0 6.0 37.0 84-85 29.032609145226225 33.0 27.0 37.0 6.0 37.0 86-87 28.84002846885776 33.0 27.0 37.0 6.0 37.0 88-89 28.73301349974868 33.0 27.0 37.0 6.0 37.0 90-91 28.61916961508506 33.0 24.5 37.0 6.0 37.0 92-93 28.40670472796152 33.0 22.0 37.0 6.0 37.0 94-95 28.191948346654087 33.0 22.0 37.0 6.0 37.0 96-97 28.053004407380172 33.0 22.0 37.0 6.0 37.0 98-99 27.909032325227784 33.0 22.0 37.0 6.0 37.0 100-101 26.67584689769368 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.016433788534211402 2306 2 -0.17133566226709718 2306 3 -0.1251724878696976 2306 4 0.1521692179696501 2306 5 0.05549646113973239 2306 6 -0.35637056275355405 2306 7 0.602745417917042 2306 8 -0.4158317718024307 2306 9 0.22388942803825174 2306 10-11 0.07464253083191608 2306 12-13 0.13476880053346818 2306 14-15 0.6283081128375869 2306 16-17 0.5176982181658474 2306 18-19 0.4396937646204222 2306 20-21 0.44657971633608895 2306 22-23 0.1954823186043555 2306 24-25 0.8057323669624452 2306 26-27 0.17550418901346632 2306 28-29 0.4408711229372173 2306 30-31 0.599806308489498 2306 32-33 0.554837539661456 2306 34-35 0.23714729843455018 2306 36-37 0.7885989226701042 2306 38-39 0.40669531507556655 2306 40-41 0.4763774489284245 2306 42-43 0.6611249052022856 2306 44-45 0.7711596681087372 2306 46-47 0.5649541023897804 2306 48-49 0.5938996709497957 2306 50-51 0.6150702479667842 2306 52-53 0.3699847308721793 2306 54-55 0.7361508972420125 2306 56-57 0.7032356961658053 2306 58-59 1.1063781287615946 2306 60-61 0.7824539131028594 2306 62-63 0.6927634718883162 2306 64-65 0.8058913650851913 2306 66-67 0.5670504570039832 2306 68-69 0.4260228776286752 2306 70-71 0.6336049145114018 2306 72-73 -0.008019355353219737 2306 74-75 0.27903351628143724 2306 76-77 0.4854216738352868 2306 78-79 0.7494516790109635 2306 80-81 0.7050942211378057 2306 82-83 0.7549632001096569 2306 84-85 0.7779038821242459 2306 86-87 0.8223559000649772 2306 88-89 0.7080732017521107 2306 90-91 0.6203929055260211 2306 92-93 0.4717220689308057 2306 94-95 0.5930340414399069 2306 96-97 0.6551164867521777 2306 98-99 0.8701054093291631 2306 100-101 0.7922696352454288 2307 1 -0.189558350827145 2307 2 -0.3529429848212793 2307 3 -0.45409599637024556 2307 4 -0.17276595937305217 2307 5 -0.42843181917262285 2307 6 -0.5561155714471298 2307 7 0.3172854438482915 2307 8 -0.622767998005294 2307 9 -0.23982308737765834 2307 10-11 -0.31335727362527876 2307 12-13 -0.15523754369563747 2307 14-15 0.24282079333003992 2307 16-17 0.07340740237043519 2307 18-19 0.12994970963448083 2307 20-21 -0.01894953575416025 2307 22-23 -0.1753556586886731 2307 24-25 0.33767087922653616 2307 26-27 -0.35783695002772475 2307 28-29 -0.05566224799154185 2307 30-31 0.11982478466546098 2307 32-33 0.1587926547316343 2307 34-35 -0.2974804729645655 2307 36-37 0.2896900122254351 2307 38-39 -0.17945111025578342 2307 40-41 0.021805359215306908 2307 42-43 0.09495308986016937 2307 44-45 0.05893298336891206 2307 46-47 -0.09582420161249772 2307 48-49 -0.009433351755646413 2307 50-51 0.1394746880978417 2307 52-53 -0.0250832038837423 2307 54-55 -0.015506360745945358 2307 56-57 -0.1284485449829873 2307 58-59 0.12920545944738748 2307 60-61 -0.05239262180666415 2307 62-63 0.11377749226361544 2307 64-65 0.10602763397574932 2307 66-67 0.07431118386887903 2307 68-69 -0.13981252116188259 2307 70-71 -0.13837340904515116 2307 72-73 -0.6202185855343814 2307 74-75 -0.34289887872288105 2307 76-77 0.006506360236787856 2307 78-79 0.08508169456927206 2307 80-81 0.21971159123114958 2307 82-83 0.3137042798332885 2307 84-85 0.32526255934702064 2307 86-87 0.11223686556629175 2307 88-89 0.0821178845919448 2307 90-91 0.10384116754115524 2307 92-93 0.1498355915667382 2307 94-95 0.3639114546530564 2307 96-97 0.29091507068183375 2307 98-99 0.16256793265625902 2307 100-101 0.12626819966445169 2201 1 -0.014632130245981756 2201 2 -0.2113286632324325 2201 3 -0.1123096433171149 2201 4 -0.04284879967241295 2201 5 -0.11163769048469874 2201 6 -0.045377862806027736 2201 7 0.45569663163735186 2201 8 -0.2862994255222233 2201 9 -0.0240337927209211 2201 10-11 -0.08160435121888199 2201 12-13 -0.05195216231678401 2201 14-15 0.292794854178986 2201 16-17 0.18101933637602485 2201 18-19 0.2012455082252771 2201 20-21 0.15896747810877088 2201 22-23 -0.007893232776169157 2201 24-25 0.44363633402122815 2201 26-27 0.19066671638522337 2201 28-29 0.1946248954571601 2201 30-31 0.5358892767072305 2201 32-33 0.23227673184067754 2201 34-35 0.05769260034311685 2201 36-37 0.42640766981112677 2201 38-39 0.10626206974562535 2201 40-41 0.23993039957019135 2201 42-43 0.27205272003121195 2201 44-45 0.4489284758430969 2201 46-47 0.35246360055009873 2201 48-49 0.35781380058996604 2201 50-51 0.2644024057560408 2201 52-53 0.22397277192837706 2201 54-55 0.3921187923480005 2201 56-57 0.5082071926818585 2201 58-59 0.7874523068781336 2201 60-61 0.47145062139584226 2201 62-63 0.5291459962477774 2201 64-65 0.4797602869775339 2201 66-67 0.431490340401421 2201 68-69 0.368204580957304 2201 70-71 0.4208209150164208 2201 72-73 0.062034859626873384 2201 74-75 0.3139074139705471 2201 76-77 0.4927645170151784 2201 78-79 0.645723294938513 2201 80-81 0.7924423432506238 2201 82-83 0.5416078811081029 2201 84-85 0.47575689010979616 2201 86-87 0.5491472896423879 2201 88-89 0.3953941877771321 2201 90-91 0.6089811320227483 2201 92-93 0.629936688058546 2201 94-95 0.3950455648505553 2201 96-97 0.3005845588464737 2201 98-99 0.4585142961543056 2201 100-101 0.5727267162709069 1101 1 0.06339385771639172 1101 2 -0.20961467986055382 1101 3 -0.04700687684859162 1101 4 0.09376561120169669 1101 5 0.12812871096995337 1101 6 0.1321908145204631 1101 7 0.38142898692375127 1101 8 -0.007218219970901885 1101 9 0.16406967225792712 1101 10-11 0.16279601542451871 1101 12-13 0.07162231274475417 1101 14-15 0.3026676468043874 1101 16-17 0.2121331026433424 1101 18-19 0.3207635559733717 1101 20-21 0.20354719199775673 1101 22-23 0.26401035690118135 1101 24-25 0.448878887508684 1101 26-27 0.3475595377757372 1101 28-29 0.3313361836522759 1101 30-31 0.4141169682200285 1101 32-33 0.27653600536012135 1101 34-35 0.20005133954688858 1101 36-37 0.4850047868414151 1101 38-39 0.21478081550724681 1101 40-41 0.24368750080201806 1101 42-43 0.14629229516206266 1101 44-45 0.2572529701558963 1101 46-47 0.3236500496485242 1101 48-49 0.505530600856261 1101 50-51 0.20011064107353604 1101 52-53 0.17732509984072564 1101 54-55 0.5619236218066561 1101 56-57 0.6204333589978646 1101 58-59 0.6838420120481423 1101 60-61 0.43354178047088965 1101 62-63 0.6824742290315555 1101 64-65 0.5887050445482487 1101 66-67 0.5139565389105023 1101 68-69 0.27069383130072566 1101 70-71 0.48299131477199 1101 72-73 0.4267717631792891 1101 74-75 0.5946827306227647 1101 76-77 0.6215889976657962 1101 78-79 0.807830103426955 1101 80-81 0.6558453206136221 1101 82-83 0.44131004544681574 1101 84-85 0.5964498858794869 1101 86-87 0.6443764541872774 1101 88-89 0.7035149913124101 1101 90-91 0.5520942723135285 1101 92-93 0.4888572262990074 1101 94-95 0.5398498354015899 1101 96-97 0.5355890673679511 1101 98-99 0.5430422427773891 1101 100-101 0.5077990389170992 2304 1 0.05031196288092232 2304 2 -0.29204919864382717 2304 3 -0.2187206915218063 2304 4 -0.28905746521918374 2304 5 -0.2807354753655318 2304 6 0.014287497647259784 2304 7 -0.20714238937810592 2304 8 -0.23917478990231444 2304 9 -0.43421869404230407 2304 10-11 -0.20082929333747046 2304 12-13 -0.23504847074843482 2304 14-15 -0.17416569812732519 2304 16-17 -0.2387157193081606 2304 18-19 -0.20041260211277034 2304 20-21 -0.2555918400374466 2304 22-23 -0.07510841503858501 2304 24-25 -0.04353344130463199 2304 26-27 -0.08973828275741624 2304 28-29 -0.1693915548145668 2304 30-31 -0.02582967684735138 2304 32-33 -0.15318822346129224 2304 34-35 -0.09872722564520586 2304 36-37 -0.11865270204028633 2304 38-39 -0.24064410484059096 2304 40-41 -0.23552715863969098 2304 42-43 -0.17905298378372336 2304 44-45 -0.21113663564032237 2304 46-47 -0.1906195430618638 2304 48-49 -0.10986289266861604 2304 50-51 -0.19291010041439094 2304 52-53 -0.20358128474381232 2304 54-55 0.0586167387548997 2304 56-57 0.26261139458647165 2304 58-59 0.08595531433608983 2304 60-61 -0.01998510110297147 2304 62-63 -0.0622331438783128 2304 64-65 0.01238209403553725 2304 66-67 0.144257506071785 2304 68-69 0.12378329856208836 2304 70-71 -0.08966361864016648 2304 72-73 0.2534258952271884 2304 74-75 0.4007965229123087 2304 76-77 0.36331726128809194 2304 78-79 0.1757477688096678 2304 80-81 0.05240763296202289 2304 82-83 0.03144253958365795 2304 84-85 -0.08701957295930285 2304 86-87 0.04787618120688464 2304 88-89 0.14154736585442507 2304 90-91 -0.038161708683304596 2304 92-93 -0.017421143394646066 2304 94-95 0.17339437281640357 2304 96-97 0.04021341262752287 2304 98-99 0.1258385233567516 2304 100-101 -0.031778365193606106 2202 1 0.35720148785388517 2202 2 0.20880165977632714 2202 3 0.2515475024419729 2202 4 0.19206214777631203 2202 5 0.23187877368550858 2202 6 0.32940122682884976 2202 7 0.46222406740689337 2202 8 0.369572445337468 2202 9 0.3495964501619859 2202 10-11 0.3564583636256131 2202 12-13 0.3960100453649069 2202 14-15 0.4954094397929012 2202 16-17 0.38937815574978174 2202 18-19 0.31837709293409944 2202 20-21 0.41314474358265585 2202 22-23 0.3946364871444743 2202 24-25 0.5031043886332043 2202 26-27 0.5184130582450024 2202 28-29 0.5321917328332688 2202 30-31 0.6393814097026009 2202 32-33 0.6709590113873602 2202 34-35 0.48333693634806707 2202 36-37 0.538247562555334 2202 38-39 0.6006363319670811 2202 40-41 0.5228164199785823 2202 42-43 0.6058762129316193 2202 44-45 0.5060875516195793 2202 46-47 0.5450850250749291 2202 48-49 0.5096872784808752 2202 50-51 0.5621658787491164 2202 52-53 0.516164676734352 2202 54-55 0.685771019056773 2202 56-57 0.6242170154344393 2202 58-59 0.652971781974788 2202 60-61 0.6868526161910502 2202 62-63 0.7773612210628649 2202 64-65 0.6143006241380995 2202 66-67 0.7290676054238894 2202 68-69 0.5842803100461502 2202 70-71 0.580400017290863 2202 72-73 0.6268420045959857 2202 74-75 0.6079162571563472 2202 76-77 0.7046342030346828 2202 78-79 0.6087807621038408 2202 80-81 0.6429128287230164 2202 82-83 0.6596975904656759 2202 84-85 0.5764638032781981 2202 86-87 0.6311047529707032 2202 88-89 0.4660651910205367 2202 90-91 0.540842557179559 2202 92-93 0.5857478438344934 2202 94-95 0.5416047801137438 2202 96-97 0.5852085203138344 2202 98-99 0.6056389065282346 2202 100-101 0.46892085105839243 1102 1 0.00804742916438883 1102 2 -0.20481364720975748 1102 3 -0.037785911912703085 1102 4 -0.23725637454559845 1102 5 -0.17664134727664305 1102 6 0.08062883099385232 1102 7 -0.22939746329753063 1102 8 0.1546641598488847 1102 9 -0.1859692537366051 1102 10-11 -0.10310716524361396 1102 12-13 -0.10945707804502902 1102 14-15 -0.25261566499938937 1102 16-17 -0.15804142596494586 1102 18-19 -0.04370798645276608 1102 20-21 -0.07985124731465731 1102 22-23 0.10173975708248406 1102 24-25 0.05480463021442006 1102 26-27 0.007450100213958422 1102 28-29 -0.12808558693810568 1102 30-31 0.08692246528081427 1102 32-33 -0.14055050634279098 1102 34-35 -0.07180145459715703 1102 36-37 -0.010869905694427473 1102 38-39 -0.14522663690880222 1102 40-41 -0.11299124310910003 1102 42-43 0.07127208020354203 1102 44-45 0.02124728916858487 1102 46-47 0.10967587359806785 1102 48-49 -0.08522624335112994 1102 50-51 -0.08129910069656887 1102 52-53 -0.08578614466795997 1102 54-55 0.29743916804571313 1102 56-57 0.5731912329122011 1102 58-59 0.2210552948198199 1102 60-61 0.1740993869430021 1102 62-63 0.23582940017313803 1102 64-65 0.4151413465499658 1102 66-67 0.31730995014275365 1102 68-69 0.29532152248207666 1102 70-71 0.31812324877475007 1102 72-73 0.4727165161056206 1102 74-75 0.5177570938315696 1102 76-77 0.45507853628476624 1102 78-79 0.3113482421724463 1102 80-81 0.2442323669129962 1102 82-83 0.05162292212740738 1102 84-85 0.2641615653737581 1102 86-87 0.4114911724369925 1102 88-89 0.19198542531565366 1102 90-91 0.21230801810134636 1102 92-93 0.2843457627296111 1102 94-95 0.2273016978816358 1102 96-97 0.21316951842311127 1102 98-99 0.2548699694129084 1102 100-101 0.2110560871139775 2305 1 0.017478079001435276 2305 2 -0.19181754180466726 2305 3 -0.1946389478855437 2305 4 -0.43264492844024716 2305 5 -0.35956232523622234 2305 6 -0.02058985840135108 2305 7 -0.3340876062758795 2305 8 0.12354923805324347 2305 9 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96-97 -0.369522909671403 1213 98-99 -0.5789002304220716 1213 100-101 -0.9018679873700393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1289.0 3 218.0 4 367.0 5 354.0 6 704.0 7 4839.0 8 25366.0 9 88813.0 10 123354.0 11 83745.0 12 47919.0 13 30396.0 14 21104.0 15 16130.0 16 13620.0 17 12285.0 18 11359.0 19 10757.0 20 11136.0 21 11550.0 22 11701.0 23 12580.0 24 13645.0 25 15825.0 26 18914.0 27 23128.0 28 29975.0 29 37627.0 30 43799.0 31 56456.0 32 78314.0 33 113325.0 34 173558.0 35 296021.0 36 534345.0 37 734378.0 38 143984.0 39 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.64156044045551 18.748253358817326 21.743804426743587 11.866381773983578 2 33.21220733784113 26.905241931945 29.796117251274246 10.086433478939629 3 38.84903346802523 17.380625001316037 30.948746186127508 12.82159534453122 4 29.438878782263995 14.092003975272457 35.027386146063186 21.441731096400357 5 39.38405491913747 12.345504829290206 31.482339398023363 16.78810085354897 6 36.86846809149972 12.480324413734225 36.71320472298319 13.938002771782857 7 41.601240129866895 17.41828658104099 25.514577035908527 15.465896253183589 8 28.703660813295716 36.50654117713115 19.88679271767662 14.90300529189652 9 38.68719969879085 27.02018739953408 18.418743181651507 15.873869720023567 10-11 32.41153093299118 30.30706514016498 22.664574866753888 14.616829060089954 12-13 36.04466014783536 21.311012267204234 22.33386420793813 20.310463377022277 14-15 30.33845402293607 21.633270000273733 25.07290788584327 22.95536809094693 16-17 33.331105088416585 23.20850424585928 26.48274347128626 16.977647194437882 18-19 32.74391293386088 23.30225607917094 27.0735816671395 16.880249319828685 20-21 29.579990859641104 28.33190204552811 25.322215194925608 16.765891899905174 22-23 35.76014226320975 23.144160812872467 23.97929919402312 17.11639772989466 24-25 28.798702881299178 29.78475734191322 24.504196824459427 16.912342952328178 26-27 23.88109493731404 32.95916975549943 25.253615902942215 17.906119404244308 28-29 26.350967991188035 30.934181032640044 23.73174532216321 18.983105654008707 30-31 30.363228065491533 25.399354148022528 25.318135231954304 18.919282554531634 32-33 24.64097105751685 28.292575356498684 27.482101486264117 19.584352099720352 34-35 33.30804516863375 23.690054616460785 24.0966861960186 18.905214018886866 36-37 26.28155023153941 25.110972324925562 27.93907083390738 20.668406609627652 38-39 19.4446309462084 30.335878935943967 29.751526922164317 20.467963195683314 40-41 23.225623105533693 32.01680794850268 24.955715600569217 19.801853345394413 42-43 29.050948028558498 25.395587788960295 27.279318231134475 18.27414595134674 44-45 23.385198347209112 29.720058983052038 27.483711995388216 19.41103067435063 46-47 26.706360354082364 30.88156340787927 24.173940834618698 18.238135403419665 48-49 20.81168114287 28.237544511512517 29.68782522234661 21.26294912327087 50-51 18.31116450639047 25.221411389824862 31.249757180982396 25.217666922802273 52-53 28.44785166169818 25.35016869171144 24.40255568250481 21.79942396408557 54-55 31.6000162408397 25.001120512479346 25.077789931066075 18.321073315614882 56-57 33.00862193833999 24.674356940653333 23.29818321935387 19.01883790165281 58-59 28.95365555293634 24.128834402201267 23.306858175174103 23.61065186968829 60-61 21.475618807291642 23.78834828362119 24.47989466167603 30.25613824741114 62-63 27.00042513158605 23.488722237932038 25.413993630583327 24.096858999898593 64-65 22.957425975239865 28.204572981092173 28.54985518184306 20.288145861824898 66-67 20.464193496622855 27.599428919634832 26.379160312199506 25.55721727154281 68-69 18.94407290633918 23.21436009667275 37.184735744926236 20.656831252061835 70-71 18.414899041636165 32.25703802284175 30.379212806954225 18.94885012856786 72-73 20.22253851185474 38.39615973395595 23.856345629566196 17.524956124623113 74-75 21.313899343143454 37.622436896842615 23.52090430185425 17.542759458159683 76-77 20.533847747073743 36.54102208279934 23.611667994401376 19.313462175725544 78-79 20.403178098731477 36.950993740049995 24.03078302172385 18.615045139494683 80-81 19.638503831441188 37.398081555840996 23.959606892904144 19.003807719813672 82-83 19.572451282361385 36.24028398246239 24.228176688167384 19.959088047008844 84-85 20.411429788921655 34.61372269405102 24.423222696160536 20.551624820866788 86-87 19.446008348228748 34.48289196677653 25.22283358507155 20.84826609992317 88-89 19.20428950351725 34.555978481464145 25.305919109135566 20.933812905883038 90-91 20.14094232552728 33.29842405615338 24.659739995891027 21.90089362242831 92-93 20.868175949088606 33.03561450108842 23.905677697516072 22.190531852306904 94-95 21.257271668297722 34.160088270278536 23.348841540442862 21.23379852098088 96-97 20.680799506506098 34.730072644874674 22.628122089491978 21.961005759127257 98-99 20.868181057740028 32.74635214794133 24.349930902041166 22.035535892277476 100-101 22.0138592463892 33.718460795252156 23.58867775521189 20.679002203146755 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1720.0 1 1041.0 2 327.0 3 461.5 4 519.5 5 372.5 6 427.0 7 579.0 8 737.0 9 870.5 10 939.5 11 1050.5 12 1137.5 13 1097.0 14 997.0 15 955.0 16 892.0 17 785.5 18 753.5 19 716.5 20 685.0 21 721.0 22 793.0 23 892.0 24 1027.0 25 1226.5 26 1461.0 27 1795.0 28 2216.5 29 2788.5 30 3538.0 31 4731.5 32 7189.5 33 11281.0 34 17688.0 35 27994.5 36 42000.0 37 61118.0 38 88858.5 39 113258.5 40 126190.0 41 129231.0 42 151551.5 43 189791.5 44 191055.5 45 175245.0 46 180582.5 47 183574.5 48 167238.5 49 144542.0 50 122743.0 51 110840.0 52 105164.0 53 103374.5 54 92957.0 55 64373.0 56 42723.0 57 35043.5 58 31340.0 59 26037.0 60 19650.0 61 14589.5 62 10283.5 63 7213.0 64 5326.5 65 3800.0 66 2665.0 67 2056.0 68 1588.5 69 1192.5 70 914.0 71 656.5 72 501.0 73 414.0 74 337.0 75 257.0 76 207.5 77 163.0 78 130.5 79 116.0 80 91.0 81 72.0 82 59.5 83 58.5 84 45.0 85 30.0 86 28.0 87 23.5 88 19.0 89 19.0 90 16.5 91 9.5 92 7.0 93 6.5 94 6.0 95 5.5 96 3.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16186799451233147 2 0.15398139885073023 3 0.12183914008767091 4 0.11658140964660339 5 0.1283587258345946 6 0.14840820458319867 7 0.12488862374349005 8 0.11574017277603259 9 0.10848450476735945 10-11 0.09691749779701095 12-13 0.12464326298957357 14-15 0.12113810936219523 16-17 0.11095563807466118 18-19 0.10895770050705553 20-21 0.10320924855815505 22-23 0.11547728625397922 24-25 0.4054586458469889 26-27 0.2904370295645688 28-29 0.3819390650072802 30-31 0.22133292580080494 32-33 0.2588380696137531 34-35 0.2712112619183986 36-37 0.26772363405915717 38-39 0.2862658967479886 40-41 0.27965868216038037 42-43 0.3335153676450485 44-45 0.2824102277578724 46-47 0.30652568471423536 48-49 0.3061401178152237 50-51 0.30654321048237226 52-53 0.3101535187185719 54-55 0.2895782669258611 56-57 0.30039166586632327 58-59 0.30065455238837663 60-61 0.2808854859299628 62-63 0.27832672378197665 64-65 0.28628342251612543 66-67 0.2859679586896614 68-69 0.283444248077949 70-71 0.2580844615838668 72-73 0.24877827870317726 74-75 0.25740095662652795 76-77 0.25387827723101275 78-79 0.2507061131982353 80-81 0.25962672917991325 82-83 0.29937517131438357 84-85 0.30459785021917724 86-87 0.293959708960084 88-89 0.301565892331495 90-91 0.2786071360721669 92-93 0.26386796506904103 94-95 0.2501628143859917 96-97 0.24926900021101026 98-99 0.27010713852577445 100-101 0.26966899432235214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2852942.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.0909487694544 #Duplication Level Percentage of deduplicated Percentage of total 1 86.4977663260887 35.54275284777548 2 5.311488952995785 4.365082409141456 3 2.3191674119251937 2.858903679336189 4 1.1850243622646655 1.9477510144149095 5 0.852087717273236 1.7506546368777944 6 0.6469968796460686 1.5951429381320077 7 0.3789182563209361 1.0899077460806204 8 0.29019392223260226 0.9539474873333516 9 0.24115795889431743 0.8918468400845327 >10 1.849582919385024 14.967259235110214 >50 0.22712740545876722 6.502242840252423 >100 0.177606789514984 14.764267395692906 >500 0.016040293262110306 4.359832468179974 >1k 0.006068037336172163 4.29967439011993 >5k 4.293152231070824E-4 1.3907434979326612 >10k+ 3.434521782324719E-4 2.7199905735353633 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 24790 0.8689275842270892 Illumina Single End PCR Primer 1 (97% over 34bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 21180 0.7423915382787312 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 19781 0.6933544390317083 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11342 0.39755452441725064 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9816 0.3440658800634573 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 9649 0.33821227350573546 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7680 0.2691957985826561 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 6498 0.22776488270704418 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5775 0.2024226219810988 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4375 0.15335047119780212 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4108 0.14399171101270197 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3996 0.14006593895003824 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3617 0.12678140670227436 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3192 0.11188450378591644 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2853 0.10000203298910389 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007290719544946936 0.0 0.0 2 0.007816492589053686 0.0 0.0 3 0.008237111024339086 0.0 0.0 4 0.008832987140993403 0.0 0.0 5 0.010445357809587436 0.0 0.0 6 0.011461852361527154 0.0 0.0 7 0.012758759203657138 0.0 0.0 8 0.01384535682814442 0.0 0.0 9 0.015668036714381155 0.0 0.0 10-11 0.024816487681838605 0.0 0.0 12-13 0.03328143369195728 0.0 0.0 14-15 0.04118555512169543 0.0 0.0 16-17 0.048669058116148176 0.0 0.0 18-19 0.05632781879196984 0.0 0.0 20-21 0.06170822960999558 0.0 0.0 22-23 0.06644018700695634 0.0 0.0 24-25 0.07189070089752964 0.0 0.0 26-27 0.07802471974544173 0.0 0.0 28-29 0.08526286198597799 0.0 0.0 30-31 0.16619685924214372 0.0 0.0 32-33 0.18016489644724637 0.0 0.0 34-35 0.18929582164656694 0.0 0.0 36-37 0.2007927255443679 0.0 0.0 38-39 0.21216694906521055 0.0 0.0 40-41 0.22588962551639674 0.0 0.0 42-43 0.24189065182537886 0.0 0.0 44-45 0.2597494095568715 0.0 0.0 46-47 0.2784669299270718 0.0 0.0 48-49 0.2989545528790981 0.0 0.0 50-51 0.32207104105165824 0.0 0.0 52-53 0.34706278641486576 0.0 0.0 54-55 0.3749462835206604 0.0 0.0 56-57 0.40456483167200735 0.0 0.0 58-59 0.43677719350761424 0.0 0.0 60-61 0.47254728627501014 0.0 1.752576813689167E-5 62-63 0.5101751104649166 0.0 3.505153627378334E-5 64-65 0.5519740674714032 0.0 3.505153627378334E-5 66-67 0.5961390031763703 0.0 3.505153627378334E-5 68-69 0.6436513605954836 0.0 3.505153627378334E-5 70-71 0.6968245411228129 0.0 3.505153627378334E-5 72-73 0.7560791631936437 0.0 3.505153627378334E-5 74-75 0.8200482168932982 0.0 3.505153627378334E-5 76-77 0.8911677839928046 0.0 3.505153627378334E-5 78-79 0.9729430181195411 0.0 3.505153627378334E-5 80-81 1.0639543320544198 0.0 3.505153627378334E-5 82-83 1.1668305910179737 0.0 3.505153627378334E-5 84-85 1.286549113161081 0.0 3.505153627378334E-5 86-87 1.4206212394083022 0.0 3.505153627378334E-5 88-89 1.5647531565660993 0.0 3.505153627378334E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4935 0.0 90.143776 9 CGGGAGA 6475 0.0 89.00096 4 GTTCGGA 900 0.0 86.96976 2 ATTCGGA 1105 0.0 86.71933 2 AGAGCGG 5310 0.0 86.45715 8 AAGCGGC 1670 0.0 85.19711 9 ATCCGGA 1150 0.0 84.97598 2 ATCGGGA 8410 0.0 83.76398 2 TCGGGAG 7020 0.0 83.10481 3 TCGGAGA 1425 0.0 80.54873 4 CGGAAAG 3025 0.0 79.18159 5 GGATCGG 2170 0.0 78.92461 1 CGGAGAG 2235 0.0 78.7326 5 AAGGGGC 4205 0.0 78.72443 9 TTGGGAG 3260 0.0 76.96553 3 TCGGGAA 2190 0.0 76.66857 3 GGAGAGC 6685 0.0 76.201065 6 GGAAAGC 2050 0.0 75.194786 6 ACCGGAA 605 0.0 74.47773 3 GTCCGGA 385 0.0 72.697266 2 >>END_MODULE