FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418366
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1619414.737380374131969No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1278643.7405005783464964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC352.806995E-454.213981
TCGGCCA652.6169364E-643.782442
CGGGTCG459.690599E-442.1664281
CGCTCGG459.690599E-442.1664281
CGCGGAG1253.6379788E-1241.7447621
CGAGGCG1500.041.1122671
CGACGCC704.3520613E-640.6604841
CGGCACC951.9566869E-839.9383663
CGACGGG1357.2759576E-1238.652561
GTGCGAC500.001626794937.9497871
CGGACGC651.3391091E-436.490181
CGCAGCC1651.8189894E-1234.4998051
CTGCGGG1255.7334546E-934.1548041
TCAGCGC702.0717987E-433.8792652
ACGCGCC702.0717987E-433.8792652
ACTGGCG2000.033.2016872
CGAGGGG7100.032.7383731
CTATGCG600.003977695531.6248251
GGACGGG4050.031.6248231
CGACACC3000.031.624821
Produced by FastQC (version 0.11.2)