##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_50110001-2.342000000358f4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6004709 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.202134691289785 33.0 33.0 33.0 33.0 33.0 2 32.19230657139255 33.0 33.0 33.0 33.0 33.0 3 32.14864866890302 33.0 33.0 33.0 33.0 33.0 4 35.94131122757156 37.0 37.0 37.0 33.0 37.0 5 35.932725132891534 37.0 37.0 37.0 33.0 37.0 6 35.93132106818165 37.0 37.0 37.0 33.0 37.0 7 35.85012695869192 37.0 37.0 37.0 33.0 37.0 8 35.7768236562338 37.0 37.0 37.0 33.0 37.0 9 35.75219864942664 37.0 37.0 37.0 33.0 37.0 10-11 35.65632797526075 37.0 37.0 37.0 33.0 37.0 12-13 35.671863948777535 37.0 37.0 37.0 33.0 37.0 14-15 37.423254232636424 40.0 37.0 40.0 33.0 40.0 16-17 37.390226154173334 40.0 37.0 40.0 33.0 40.0 18-19 37.37959533093111 40.0 37.0 40.0 33.0 40.0 20-21 37.34530174567993 40.0 37.0 40.0 33.0 40.0 22-23 37.30301593299525 40.0 37.0 40.0 33.0 40.0 24-25 37.096799278699436 40.0 37.0 40.0 33.0 40.0 26-27 37.0275676140176 40.0 37.0 40.0 33.0 40.0 28-29 36.948309068765866 40.0 37.0 40.0 33.0 40.0 30-31 37.00543973071801 40.0 37.0 40.0 33.0 40.0 32-33 36.92392004341926 40.0 37.0 40.0 33.0 40.0 34-35 36.837824530714144 40.0 37.0 40.0 33.0 40.0 36-37 36.7693487727715 40.0 37.0 40.0 33.0 40.0 38-39 36.728570776702085 40.0 37.0 40.0 33.0 40.0 40-41 36.60058114389889 40.0 37.0 40.0 33.0 40.0 42-43 36.46473667583225 40.0 37.0 40.0 33.0 40.0 44-45 36.3542752030115 37.0 37.0 40.0 33.0 40.0 46-47 36.21584609678837 37.0 37.0 40.0 33.0 40.0 48-49 36.0740638722043 37.0 37.0 40.0 30.0 40.0 50-51 34.87044001632719 37.0 35.0 38.5 27.0 38.5 52-53 35.29467789363315 37.0 35.0 38.5 27.0 40.0 54-55 36.01694570044943 37.0 37.0 40.0 33.0 40.0 56-57 36.060008320136745 37.0 37.0 40.0 33.0 40.0 58-59 35.86262839714631 37.0 37.0 40.0 27.0 40.0 60-61 35.631849020493746 37.0 37.0 40.0 27.0 40.0 62-63 35.35238477001967 37.0 37.0 40.0 27.0 40.0 64-65 35.06438072186346 37.0 35.0 38.5 27.0 40.0 66-67 34.81307911840524 37.0 33.0 37.0 27.0 40.0 68-69 34.59708230656973 37.0 33.0 37.0 27.0 40.0 70-71 34.33345795774616 37.0 33.0 37.0 27.0 40.0 72-73 34.09243453762706 37.0 33.0 37.0 27.0 38.5 74-75 33.867875195950376 37.0 33.0 37.0 27.0 37.0 76-77 33.64828562050218 37.0 33.0 37.0 27.0 37.0 78-79 33.44079496608411 37.0 33.0 37.0 27.0 37.0 80-81 33.21410962629496 37.0 33.0 37.0 27.0 37.0 82-83 33.031815613379436 37.0 33.0 37.0 27.0 37.0 84-85 32.8843819908675 37.0 33.0 37.0 27.0 37.0 86-87 32.76149526979576 37.0 33.0 37.0 27.0 37.0 88-89 32.61569711371526 37.0 33.0 37.0 24.5 37.0 90-91 32.47930440925613 33.0 33.0 37.0 22.0 37.0 92-93 32.34326367855628 33.0 33.0 37.0 22.0 37.0 94-95 32.20446619478146 33.0 33.0 37.0 22.0 37.0 96-97 32.07814675115813 33.0 33.0 37.0 22.0 37.0 98-99 31.907046952649992 33.0 33.0 37.0 22.0 37.0 100-101 30.555042634039385 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3034171779627073 2306 2 0.18405805744543358 2306 3 0.31036844819552556 2306 4 0.3294254615290697 2306 5 0.3445976107488917 2306 6 0.3185467331698959 2306 7 0.32824657328838924 2306 8 0.31935534312651015 2306 9 0.3735425043011418 2306 10-11 0.3792766478305296 2306 12-13 0.35994522202277324 2306 14-15 0.6307438140092145 2306 16-17 0.5977797431557477 2306 18-19 0.4986596690814835 2306 20-21 0.5425707217935312 2306 22-23 0.6261551643855867 2306 24-25 0.7866783218072158 2306 26-27 0.6940837492940588 2306 28-29 0.6030198689532753 2306 30-31 0.5489488845714874 2306 32-33 0.5213903423643629 2306 34-35 0.567074217758524 2306 36-37 0.4843474799459955 2306 38-39 0.5163506657183916 2306 40-41 0.5000653237261901 2306 42-43 0.4598839516673294 2306 44-45 0.551293451497628 2306 46-47 0.39491382479994286 2306 48-49 0.6266159325618119 2306 50-51 0.5698007954794733 2306 52-53 0.6550744505248289 2306 54-55 0.7620977321859996 2306 56-57 0.5925431613795027 2306 58-59 0.6666546053319209 2306 60-61 0.6701073063673562 2306 62-63 0.5395467925831454 2306 64-65 0.5329435311769117 2306 66-67 0.5159641741881131 2306 68-69 0.496235099477353 2306 70-71 0.4541832949192255 2306 72-73 0.4421318571194064 2306 74-75 0.5022546763613107 2306 76-77 0.6188911360371776 2306 78-79 0.507161147308338 2306 80-81 0.4995323104578375 2306 82-83 0.5430633031267433 2306 84-85 0.5602854867684144 2306 86-87 0.47825124607148695 2306 88-89 0.5347041987128591 2306 90-91 0.4611965388474246 2306 92-93 0.4421316927935379 2306 94-95 0.5052174992328489 2306 96-97 0.49870377983064174 2306 98-99 0.5800531344973194 2306 100-101 0.5216027131717738 2307 1 0.2924507485696637 2307 2 0.2686029251161699 2307 3 0.2721752754481912 2307 4 0.31680643685793797 2307 5 0.27222413036687243 2307 6 0.380153905692616 2307 7 0.4092589444127839 2307 8 0.3486741363453376 2307 9 0.36419143571860246 2307 10-11 0.298423484260006 2307 12-13 0.3645176750520136 2307 14-15 0.5342366596918424 2307 16-17 0.5334159569156469 2307 18-19 0.5074390952258483 2307 20-21 0.4610927825291 2307 22-23 0.3771156406572729 2307 24-25 0.6161502266715004 2307 26-27 0.49256133735728724 2307 28-29 0.4230550604677674 2307 30-31 0.48325723374182417 2307 32-33 0.36731087533347306 2307 34-35 0.3207247744769788 2307 36-37 0.400514295852588 2307 38-39 0.2763963795396549 2307 40-41 0.31847875647225266 2307 42-43 0.29812572692765826 2307 44-45 0.2013261414929559 2307 46-47 0.07209560396494652 2307 48-49 0.21339860271796596 2307 50-51 0.15426150117148296 2307 52-53 0.2300973994232649 2307 54-55 0.29827368097158313 2307 56-57 0.36897648930340665 2307 58-59 0.2002226311390345 2307 60-61 0.1889021452747457 2307 62-63 0.17087141425926688 2307 64-65 0.35039924728550886 2307 66-67 0.3780074512935556 2307 68-69 0.23091809988347478 2307 70-71 0.2771361200020763 2307 72-73 0.26469566988495075 2307 74-75 0.36384358861784705 2307 76-77 0.3395556614473705 2307 78-79 0.3858565240560736 2307 80-81 0.30190144956454645 2307 82-83 0.3073783495586184 2307 84-85 0.3470462995232495 2307 86-87 0.26591617540425716 2307 88-89 0.27239185040693314 2307 90-91 0.3174304976154687 2307 92-93 0.28872101830872765 2307 94-95 0.28095800794464765 2307 96-97 0.327828876932692 2307 98-99 0.23963446091756424 2307 100-101 0.2467630259522835 2201 1 0.20690824028825006 2201 2 0.23242553029079005 2201 3 0.2721859808046645 2201 4 0.34723940911000994 2201 5 0.3038414531088378 2201 6 0.3655606608340207 2201 7 0.3255566305652309 2201 8 0.34867290702210596 2201 9 0.34894490800609645 2201 10-11 0.3175934738983486 2201 12-13 0.34818865140152866 2201 14-15 0.4422936543950442 2201 16-17 0.42837986526611616 2201 18-19 0.4651400548714122 2201 20-21 0.45540703452919473 2201 22-23 0.3605103266105161 2201 24-25 0.6468550330231295 2201 26-27 0.5236504864950433 2201 28-29 0.4970818065459852 2201 30-31 0.45971659024130673 2201 32-33 0.30234290970413014 2201 34-35 0.4340679410302428 2201 36-37 0.49791549629696874 2201 38-39 0.4364136966062446 2201 40-41 0.44388278763826605 2201 42-43 0.4116701951600774 2201 44-45 0.5081665262158026 2201 46-47 0.45873435611581925 2201 48-49 0.4997092894758097 2201 50-51 0.46709516958930664 2201 52-53 0.4948234239963156 2201 54-55 0.6063415705519262 2201 56-57 0.5981107122945417 2201 58-59 0.7212547125312909 2201 60-61 0.5783126115380881 2201 62-63 0.5913320242586693 2201 64-65 0.5701453080344692 2201 66-67 0.5129092849674564 2201 68-69 0.5457381241451955 2201 70-71 0.5216642614389926 2201 72-73 0.6401141071825762 2201 74-75 0.5639195044495722 2201 76-77 0.5149961189973808 2201 78-79 0.5043558307946014 2201 80-81 0.5379681848103743 2201 82-83 0.5053257690478077 2201 84-85 0.5707981612436441 2201 86-87 0.5638107237501373 2201 88-89 0.7010053064663282 2201 90-91 0.6748527696844704 2201 92-93 0.5416307367593944 2201 94-95 0.4864330857979482 2201 96-97 0.5289760199061107 2201 98-99 0.49197295384001904 2201 100-101 0.44392731532124685 1101 1 0.25377310238952333 1101 2 0.1907390792006609 1101 3 0.23789478478601467 1101 4 0.26346064966703864 1101 5 0.30730209126276975 1101 6 0.2332320796226952 1101 7 0.2903550450620145 1101 8 0.30291922099922886 1101 9 0.32019109082462194 1101 10-11 0.3558701308020389 1101 12-13 0.31611427590432584 1101 14-15 0.47645613985155677 1101 16-17 0.44441379288068816 1101 18-19 0.4669337876745061 1101 20-21 0.4815881704945326 1101 22-23 0.4753224783940695 1101 24-25 0.5770383801361163 1101 26-27 0.5851640605632582 1101 28-29 0.5395667433144453 1101 30-31 0.41441762818324435 1101 32-33 0.4537020113251202 1101 34-35 0.426783436360175 1101 36-37 0.5438958852137716 1101 38-39 0.4522163547853566 1101 40-41 0.4649201147369979 1101 42-43 0.3955483969294775 1101 44-45 0.30084735349235103 1101 46-47 0.4113603952511724 1101 48-49 0.43132994376423284 1101 50-51 0.3823599080079205 1101 52-53 0.4543135399761056 1101 54-55 0.6327488444782219 1101 56-57 0.5685263259496907 1101 58-59 0.5618308867279964 1101 60-61 0.5209774700426237 1101 62-63 0.589421185558237 1101 64-65 0.5695674051428625 1101 66-67 0.6044103721338843 1101 68-69 0.4521545292714393 1101 70-71 0.5266876218881649 1101 72-73 0.5939067093664718 1101 74-75 0.5258027000381276 1101 76-77 0.5838601771990284 1101 78-79 0.5842104951966363 1101 80-81 0.5207526292679958 1101 82-83 0.591812911314598 1101 84-85 0.6596236081597695 1101 86-87 0.5878823293809745 1101 88-89 0.5622532490510679 1101 90-91 0.5016876555006959 1101 92-93 0.5043298838706676 1101 94-95 0.5929691107732538 1101 96-97 0.591001093702836 1101 98-99 0.5415935354403985 1101 100-101 0.5578309136523956 2304 1 0.25726184095461235 2304 2 0.2785603264373222 2304 3 0.25646500435163944 2304 4 0.309283392927604 2304 5 0.3176280378089942 2304 6 0.28216865370991684 2304 7 0.4174654509948894 2304 8 0.30663470773227886 2304 9 0.3207371530759886 2304 10-11 0.3326319379159486 2304 12-13 0.34906375150691815 2304 14-15 0.5320266646637677 2304 16-17 0.5363552829259035 2304 18-19 0.48247708376955245 2304 20-21 0.48238728983332635 2304 22-23 0.4574837008953878 2304 24-25 0.5230056533732963 2304 26-27 0.5983385394234659 2304 28-29 0.4847001342704047 2304 30-31 0.3689651034963717 2304 32-33 0.38430855027516486 2304 34-35 0.4760258657262497 2304 36-37 0.44946289315244314 2304 38-39 0.4407369413457545 2304 40-41 0.4905481810111283 2304 42-43 0.38935763990440364 2304 44-45 0.33227880218725403 2304 46-47 0.44195444479129975 2304 48-49 0.5063843538560349 2304 50-51 0.39049522557935035 2304 52-53 0.4190590017438609 2304 54-55 0.6076722735742237 2304 56-57 0.6245289978718276 2304 58-59 0.6778544615037205 2304 60-61 0.5576401554280821 2304 62-63 0.631708028077604 2304 64-65 0.6252426020314203 2304 66-67 0.572733598964561 2304 68-69 0.6490682472876088 2304 70-71 0.6584109259998954 2304 72-73 0.5548815641714455 2304 74-75 0.5943451453203039 2304 76-77 0.6230876553436886 2304 78-79 0.564594884967228 2304 80-81 0.6117503121893861 2304 82-83 0.6616967056965279 2304 84-85 0.6124812257304342 2304 86-87 0.6667778868031746 2304 88-89 0.6497695653153812 2304 90-91 0.5746166791309193 2304 92-93 0.6203177488533811 2304 94-95 0.6151768371640998 2304 96-97 0.6135919113263384 2304 98-99 0.6546261445232204 2304 100-101 0.5871827628741073 2202 1 0.16816557366364293 2202 2 0.2173290334711666 2202 3 0.23258373674281074 2202 4 0.2661384465173171 2202 5 0.24220146541448884 2202 6 0.26980556130560984 2202 7 0.2077003553105854 2202 8 0.2891903788715524 2202 9 0.23529495746051055 2202 10-11 0.23824329685408685 2202 12-13 0.3171545849095807 2202 14-15 0.39782771399313077 2202 16-17 0.3629697658950022 2202 18-19 0.3551872434829022 2202 20-21 0.3878839122290927 2202 22-23 0.3690810653685972 2202 24-25 0.4559234917474697 2202 26-27 0.39982414563939983 2202 28-29 0.4301396684396295 2202 30-31 0.45768970860785885 2202 32-33 0.4646048947521706 2202 34-35 0.3446180812617001 2202 36-37 0.4413971165916877 2202 38-39 0.338168972771264 2202 40-41 0.3382637627568741 2202 42-43 0.3377668329731094 2202 44-45 0.25471170747472627 2202 46-47 0.36726363350602753 2202 48-49 0.33287251011359587 2202 50-51 0.4492659276758175 2202 52-53 0.5345823060581623 2202 54-55 0.5410003671426651 2202 56-57 0.554323806906929 2202 58-59 0.5137116035724105 2202 60-61 0.5531575604075911 2202 62-63 0.4948738572336069 2202 64-65 0.48763278926682574 2202 66-67 0.5234874403288714 2202 68-69 0.5200344238216275 2202 70-71 0.57457612536205 2202 72-73 0.610488395380365 2202 74-75 0.49222128259287956 2202 76-77 0.5788032133776326 2202 78-79 0.49108140592754523 2202 80-81 0.49110251780675895 2202 82-83 0.5468120871592461 2202 84-85 0.5169299496568911 2202 86-87 0.6333786986873662 2202 88-89 0.5993393901566577 2202 90-91 0.5036929181189862 2202 92-93 0.616903779694681 2202 94-95 0.5202250610575518 2202 96-97 0.566077818641368 2202 98-99 0.46594090919270315 2202 100-101 0.6040647947799833 1102 1 0.16860021558579064 1102 2 0.06392511558938452 1102 3 0.17475067802477895 1102 4 0.10630048699902517 1102 5 0.13390715012916843 1102 6 0.06501240872371739 1102 7 0.06771274886194334 1102 8 0.1351660575208058 1102 9 0.062378092251250905 1102 10-11 0.20467014653907967 1102 12-13 0.15842258755918692 1102 14-15 0.07693250580106081 1102 16-17 0.12589055477251776 1102 18-19 0.13632590300144898 1102 20-21 0.21779652307029806 1102 22-23 0.17565013152206888 1102 24-25 0.16271385749135447 1102 26-27 0.20664252425218876 1102 28-29 0.19520947022473223 1102 30-31 0.2626093200737927 1102 32-33 0.21619220712099718 1102 34-35 0.2589343752420987 1102 36-37 0.19589741820358597 1102 38-39 0.1417600124002476 1102 40-41 0.12650420650228966 1102 42-43 0.14220130054074076 1102 44-45 0.13212600698655308 1102 46-47 0.2887389369162676 1102 48-49 0.2749471409730049 1102 50-51 0.12958019169109747 1102 52-53 0.3064553911699619 1102 54-55 0.4342835040696258 1102 56-57 0.422368659022041 1102 58-59 0.3993285336046384 1102 60-61 0.36147779381867196 1102 62-63 0.40761733094203834 1102 64-65 0.48140087651847807 1102 66-67 0.44675219363778496 1102 68-69 0.48147804890808743 1102 70-71 0.4116074235556155 1102 72-73 0.43583303161594245 1102 74-75 0.34080723244534994 1102 76-77 0.4815447724355053 1102 78-79 0.372646978249918 1102 80-81 0.3582586250148765 1102 82-83 0.1970937661657146 1102 84-85 0.41705221559794836 1102 86-87 0.46012071181367276 1102 88-89 0.44772573449143493 1102 90-91 0.41700100461224565 1102 92-93 0.31613965817897594 1102 94-95 0.336987316800041 1102 96-97 0.4069731229289104 1102 98-99 0.42867969738240674 1102 100-101 0.4858744599871372 2305 1 0.10579468633960687 2305 2 0.11492069852815234 2305 3 0.09391509951426258 2305 4 0.14335188281152966 2305 5 0.13368880406076755 2305 6 0.19411743364261724 2305 7 0.13412040557162896 2305 8 0.17929977792774565 2305 9 0.10650147801983678 2305 10-11 0.15548674396827522 2305 12-13 0.18240385518384983 2305 14-15 0.12255936678346302 2305 16-17 0.15714588979955835 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#Quality Count 2 2859.0 3 241.0 4 195.0 5 306.0 6 660.0 7 2688.0 8 8880.0 9 12767.0 10 16340.0 11 16198.0 12 14155.0 13 12992.0 14 12795.0 15 13413.0 16 14335.0 17 15135.0 18 16306.0 19 17434.0 20 19446.0 21 21980.0 22 24763.0 23 27292.0 24 31284.0 25 36226.0 26 43057.0 27 51538.0 28 62560.0 29 75284.0 30 94113.0 31 122673.0 32 167742.0 33 240916.0 34 366097.0 35 610098.0 36 1169296.0 37 2039548.0 38 622565.0 39 532.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.765904739217426 28.87852500294435 16.500415211591093 16.85515504624713 2 33.61871184504589 23.75993375771189 28.335575771789713 14.285778625452505 3 39.66877101801436 21.165464581180498 21.23268220670579 17.933082194099356 4 33.36542585227481 20.517057501745803 27.531596179845273 18.585920466134112 5 34.96416989063441 18.580276838478756 27.193010981585402 19.26254228930143 6 31.656253758162876 19.298305017461228 29.46004383779715 19.585397386578748 7 36.04603074919949 19.576875833573183 22.749881853143037 21.627211564084288 8 30.79932142367634 26.109714733156565 22.545938463252064 20.545025379915035 9 29.51558809774105 23.12922402560321 26.517131193118676 20.838056683537065 10-11 22.826257200102994 30.283472940499433 31.050739288233096 15.83953057116448 12-13 23.1849645527971 29.06088654402049 31.221828943430975 16.532319959751433 14-15 22.83947318285981 28.71329336584426 31.705227192288753 16.742006259007173 16-17 22.249121182376726 29.026858814111833 31.84721186845222 16.876808135059218 18-19 21.413604285603647 29.478447558269167 31.682523263441926 17.425424892685257 20-21 21.735488668767182 29.49329077280257 30.80188371361971 17.96933684481053 22-23 21.562876497517507 28.140360817337733 31.34335189640739 18.953410788737365 24-25 20.696789011883823 28.419966202941726 31.681067083289122 19.20217770188533 26-27 20.61662204884939 28.891732840319882 31.454972363624034 19.036672747206694 28-29 20.64185261019242 28.946164857047723 30.767974898428633 19.644007634331217 30-31 20.130086194183903 30.538667539950175 30.20015334204113 19.13109292382479 32-33 19.40363404295607 31.051867394342423 31.416253516227975 18.12824504647353 34-35 19.662534857935103 30.56725175643412 32.20640657054698 17.563806815083794 36-37 19.75013474354473 30.14416202457238 32.810677283052456 17.295025948830435 38-39 20.163782155835907 29.611588619765865 31.84055993533966 18.38406928905857 40-41 20.22947183648451 29.484028663311314 31.10335877824347 19.183140721960704 42-43 20.57973872319448 28.807777308940814 31.281852249512248 19.33063171835246 44-45 20.08063567211968 28.844418281661326 31.608201392263506 19.466744653955484 46-47 20.618152818862825 29.14936472559987 30.910806810976222 19.321675644561083 48-49 20.452898741394776 29.90106674966374 30.193703281775907 19.45233122716557 50-51 21.113857089611397 29.826254906884266 28.850441291720312 20.209446711784025 52-53 21.43713023040409 29.711983191334234 28.750791080554222 20.100095497707454 54-55 21.817233527328032 29.635129339773563 28.77243824901169 19.77519888388672 56-57 21.72992326750495 30.31035456850939 28.617822793759096 19.34189937022656 58-59 21.215102362616438 31.23517136751805 28.38077368114597 19.16895258871954 60-61 20.874168276570682 31.28136055998772 28.076230534803237 19.76824062863836 62-63 20.79536059139817 31.049973749800312 28.933924568942658 19.220741089858855 64-65 20.665429725032 30.166933672240976 29.68021047327173 19.487426129455294 66-67 21.1041136182754 29.39750946415314 29.32915376733007 20.169223150241393 68-69 21.269009237708076 30.00082592126757 29.891618920094164 18.838545920930187 70-71 20.597052108645826 30.861131050807238 29.68337543628695 18.858441404259988 72-73 20.395835088136106 30.619727191577883 29.480853581705162 19.503584138580848 74-75 20.589433828243376 29.63844063387914 29.252879498495016 20.519246039382473 76-77 20.556846775095714 29.07737633094402 28.766348258606783 21.59942863535348 78-79 19.893135939666593 29.748020873595536 29.09104190163097 21.267801285106902 80-81 19.43317522661536 30.284396727080733 28.784183495074622 21.49824455122928 82-83 18.709620191017468 30.46879522114715 28.797578232558468 22.024006355276917 84-85 18.450674801601537 30.791093793292053 29.85637594398342 20.901855461122988 86-87 18.946518437422377 30.43562399555132 30.549045994040252 20.068811572986053 88-89 19.774921507605256 30.206982371379514 29.96360144631984 20.05449467469539 90-91 19.744663538433286 30.33473088110144 29.415592771721606 20.50501280874367 92-93 19.18976280588556 30.860065575084473 29.526112726692833 20.424058892337133 94-95 19.34595694503866 30.245436236708372 29.73694107334787 20.671665744905095 96-97 19.726729654778623 30.825420862210308 29.350629185456523 20.09722029755455 98-99 20.554821834330152 30.56261977360008 28.73059251229163 20.15196587977814 100-101 20.322733663024653 31.064974996770694 27.939306225411237 20.672985114793416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14179.0 1 8290.0 2 1921.5 3 1467.0 4 1307.0 5 1114.0 6 1209.5 7 1485.0 8 1769.0 9 2041.0 10 2398.5 11 2727.5 12 2803.0 13 2654.0 14 2472.0 15 2257.5 16 2052.0 17 1961.5 18 2005.0 19 2180.0 20 2613.0 21 3162.5 22 3913.5 23 5228.5 24 6962.0 25 9201.5 26 11921.0 27 15611.5 28 20380.5 29 26102.5 30 33872.5 31 43309.0 32 60481.0 33 94351.0 34 141445.5 35 185445.5 36 241511.5 37 324495.0 38 403893.0 39 448821.0 40 453146.5 41 441710.0 42 442007.0 43 434228.0 44 384222.5 45 319379.0 46 277719.5 47 243031.5 48 205354.0 49 164998.0 50 120311.0 51 89647.5 52 72566.0 53 55940.5 54 40613.0 55 29631.0 56 22266.0 57 17019.0 58 12894.5 59 9656.5 60 7161.0 61 5718.5 62 4587.5 63 3563.5 64 2699.5 65 2020.0 66 1584.0 67 1295.0 68 1110.5 69 968.0 70 804.5 71 638.5 72 501.5 73 389.0 74 292.5 75 226.0 76 176.0 77 135.0 78 113.5 79 94.0 80 75.5 81 55.5 82 39.0 83 32.0 84 28.0 85 28.0 86 25.0 87 15.0 88 13.5 89 12.5 90 10.5 91 9.5 92 5.5 93 4.0 94 3.0 95 2.0 96 2.5 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1694503430557584 2 0.16232260380977662 3 0.1293984437880337 4 0.12431909689545322 5 0.13682594776865958 6 0.1569767993752903 7 0.1321462871889379 8 0.12401933216080911 9 0.11697486089667292 10-11 0.10442637603254379 12-13 0.13278745064914887 14-15 0.12909035225520504 16-17 0.11927305719561097 18-19 0.11736622041134716 20-21 0.1109712394056065 22-23 0.12337816870059815 24-25 0.41957903372170074 26-27 0.30183810739204847 28-29 0.3925169396218868 30-31 0.22528152488322084 32-33 0.2642842475796912 34-35 0.27734066713307837 36-37 0.27447624855759034 38-39 0.29530157081717034 40-41 0.28750768771642393 42-43 0.34363030748034584 44-45 0.28991413239176117 46-47 0.316226814654965 48-49 0.3156855727729687 50-51 0.31618518066404216 52-53 0.32016539019626095 54-55 0.2974582115469709 56-57 0.30955704930913386 58-59 0.3099650624201772 60-61 0.2879739884147592 62-63 0.28536770058299243 64-65 0.293611230785705 66-67 0.29383605433668814 68-69 0.2906968514211097 70-71 0.26342658736668173 72-73 0.25304307003053766 74-75 0.26271880952099425 76-77 0.2587802339796983 78-79 0.25519971076033826 80-81 0.2652418293709154 82-83 0.3081331668195744 84-85 0.3137287751995975 86-87 0.3033619114598226 88-89 0.31008163759476104 90-91 0.28640022355787764 92-93 0.2690221957467048 94-95 0.2543253969509596 96-97 0.25345108314158105 98-99 0.2762581833690858 100-101 0.27615826179087116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6004709.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.642112790474584 #Duplication Level Percentage of deduplicated Percentage of total 1 81.18088603938526 29.746391826858183 2 8.55976018599098 6.272953963889903 3 3.266203342507444 3.5904177381834863 4 1.616796627254869 2.3697137750052724 5 0.9753307231831013 1.7869089183445173 6 0.7179952426091019 1.5785317597624124 7 0.5383671518157074 1.3808836929662394 8 0.37337179346859195 1.0944905095246344 9 0.2717493046573888 0.8961721804790173 >10 2.0227049451676162 14.426468457305022 >50 0.24396452993956816 6.121953040965398 >100 0.19175754628686842 14.562769548520802 >500 0.0274191054182777 6.899062566864362 >1k 0.013465328351993036 8.327929224808027 >5k 1.368808357680058E-4 0.40248518464605243 >10k+ 9.125312738423378E-5 0.5428676118767869 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 20453 0.3406160065375358 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12018 0.20014292116403973 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 9089 0.1513645373988981 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7761 0.12924856142071164 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 7224 0.1203055801704962 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.661438547646522E-4 0.0 0.0 2 7.161046438720011E-4 0.0 0.0 3 8.326798184558153E-4 0.0 0.0 4 9.326013966705131E-4 0.0 0.0 5 9.992157821469784E-4 0.0 0.0 6 0.0011657517458381413 0.0 0.0 7 0.0013989020950057696 0.0 0.0 8 0.0017486276187572121 0.0 0.0 9 0.0020483923534013056 0.0 0.0 10-11 0.0031475297137629815 0.0 0.0 12-13 0.005129307681687822 0.0 0.0 14-15 0.008318471386373594 0.0 0.0 16-17 0.014230498097409883 0.0 0.0 18-19 0.021566407298005616 0.0 0.0 20-21 0.027928081111008043 0.0 0.0 22-23 0.0356969838172008 0.0 0.0 24-25 0.0442319519563729 0.0 0.0 26-27 0.054115861401443435 0.0 0.0 28-29 0.0647491826831242 0.0 0.0 30-31 0.07743922311639082 0.0 0.0 32-33 0.09088700218445224 0.0 0.0 34-35 0.1056587421638584 0.0 0.0 36-37 0.12166284827457917 0.0 0.0 38-39 0.13814990868000432 0.0 0.0 40-41 0.15614411955683447 0.0 0.0 42-43 0.17607014761248213 0.0 0.0 44-45 0.19612107764089817 0.0 0.0 46-47 0.21767083134253468 0.0 0.0 48-49 0.24070275512102252 0.0 0.0 50-51 0.26624104515306235 0.0 0.0 52-53 0.2917043940014412 0.0 0.0 54-55 0.3191578476159294 0.0 0.0 56-57 0.3491509746767079 0.0 0.0 58-59 0.3799101671704657 0.0 0.0 60-61 0.4128010199994704 0.0 0.0 62-63 0.44833146785298006 0.0 0.0 64-65 0.48702609901662175 0.0 0.0 66-67 0.5311164954038572 0.0 0.0 68-69 0.5810189969239141 0.0 0.0 70-71 0.6361174205111355 0.0 0.0 72-73 0.6988015572444892 0.0 0.0 74-75 0.7736927801164053 0.0 0.0 76-77 0.8613573114034336 0.0 0.0 78-79 0.9722952436163018 0.0 0.0 80-81 1.108996289412193 0.0 0.0 82-83 1.2769894427856538 0.0 0.0 84-85 1.4848263254722252 0.0 0.0 86-87 1.7297840744655568 0.0 0.0 88-89 2.0163341803907566 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 865 0.0 52.07323 4 CGACGGG 330 0.0 51.736057 1 CGCGGAG 355 0.0 48.092674 1 CGAGTGG 315 0.0 45.1664 1 ATTCGGA 200 0.0 45.048603 2 GGACGGG 845 0.0 43.776665 1 CGTACCC 55 5.0125214E-5 43.113384 1 GGTACGG 200 0.0 42.68225 1 CGGACGG 105 1.0440999E-9 40.64976 1 GGTCCGG 170 0.0 39.055656 1 CACGGGG 635 0.0 38.83599 1 GATCGGG 1815 0.0 37.62623 1 CAGCGGG 325 0.0 36.480556 1 GAGCGGC 675 0.0 35.82126 9 GTTACGG 350 0.0 33.8748 1 CGAGGGG 1090 0.0 33.50187 1 CGCGGGG 690 0.0 32.99111 1 ACTGGCG 795 0.0 32.805996 2 CGCAGCC 485 0.0 32.268368 1 TGTACCG 250 0.0 32.245316 2 >>END_MODULE