FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6004709
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC204530.3406160065375358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120180.20014292116403973No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG90890.1513645373988981No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA77610.12924856142071164No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT72240.1203055801704962Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA8650.052.073234
CGACGGG3300.051.7360571
CGCGGAG3550.048.0926741
CGAGTGG3150.045.16641
ATTCGGA2000.045.0486032
GGACGGG8450.043.7766651
CGTACCC555.0125214E-543.1133841
GGTACGG2000.042.682251
CGGACGG1051.0440999E-940.649761
GGTCCGG1700.039.0556561
CACGGGG6350.038.835991
GATCGGG18150.037.626231
CAGCGGG3250.036.4805561
GAGCGGC6750.035.821269
GTTACGG3500.033.87481
CGAGGGG10900.033.501871
CGCGGGG6900.032.991111
ACTGGCG7950.032.8059962
CGCAGCC4850.032.2683681
TGTACCG2500.032.2453162
Produced by FastQC (version 0.11.2)