FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892301
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT50830.13059113362507163Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGA250.00504792656.9263881
CGACGCC804.278263E-947.4386561
CGGGAGA6000.044.2675364
GAGCGGC4000.043.8689049
CGGACCG459.6893334E-442.1676941
GATCCGG1253.6379788E-1241.7460171
CGAGGCG1500.041.1135031
GATTCGC704.3609452E-640.6486327
CGGCGAC951.9525032E-839.9483451
GTGTCGC756.988228E-637.9509281
CGCGCCG500.00162658337.9509241
CGGAGCG500.00162658337.9509241
CGGGCCA1203.8471626E-935.5789951
CGGGTGC1353.1286618E-1035.1397481
GTGACCC2850.034.95481
CGAGGGG3900.034.0585251
CGAGCGC702.0698809E-433.8847581
CACCGAG1703.6379788E-1233.486111
CGAGTGG2450.032.916621
GCGGCCC1308.418283E-932.8358083
Produced by FastQC (version 0.11.2)