##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_44065-1.342000000357db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3892301 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21888415104587 33.0 33.0 33.0 33.0 33.0 2 32.22811519458541 33.0 33.0 33.0 33.0 33.0 3 32.183708813886696 33.0 33.0 33.0 33.0 33.0 4 36.0030660527025 37.0 37.0 37.0 33.0 37.0 5 35.98944351939894 37.0 37.0 37.0 33.0 37.0 6 36.0020147465471 37.0 37.0 37.0 33.0 37.0 7 35.94291088998513 37.0 37.0 37.0 33.0 37.0 8 35.89651468373078 37.0 37.0 37.0 33.0 37.0 9 35.885935337477754 37.0 37.0 37.0 33.0 37.0 10-11 35.82510563802748 37.0 37.0 37.0 33.0 37.0 12-13 35.81071260932801 37.0 37.0 37.0 33.0 37.0 14-15 37.62396292064771 40.0 37.0 40.0 33.0 40.0 16-17 37.59623253700061 40.0 37.0 40.0 33.0 40.0 18-19 37.589419600385476 40.0 37.0 40.0 33.0 40.0 20-21 37.55331961736772 40.0 37.0 40.0 33.0 40.0 22-23 37.53179173450357 40.0 37.0 40.0 33.0 40.0 24-25 37.322436651225075 40.0 37.0 40.0 33.0 40.0 26-27 37.251790778770705 40.0 37.0 40.0 33.0 40.0 28-29 37.16513869302503 40.0 37.0 40.0 33.0 40.0 30-31 37.216716923999456 40.0 37.0 40.0 33.0 40.0 32-33 37.14781552094763 40.0 37.0 40.0 33.0 40.0 34-35 37.082371712773494 40.0 37.0 40.0 33.0 40.0 36-37 37.01779281715366 40.0 37.0 40.0 33.0 40.0 38-39 36.98004136884583 40.0 37.0 40.0 33.0 40.0 40-41 36.86506259407996 40.0 37.0 40.0 33.0 40.0 42-43 36.75132421670369 40.0 37.0 40.0 33.0 40.0 44-45 36.64927905113196 40.0 37.0 40.0 33.0 40.0 46-47 36.52295069163459 40.0 37.0 40.0 33.0 40.0 48-49 36.3911980342733 37.0 37.0 40.0 33.0 40.0 50-51 35.22306638155682 37.0 35.0 38.5 30.0 38.5 52-53 35.64297905018137 37.0 37.0 38.5 33.0 40.0 54-55 36.376849195373126 37.0 37.0 40.0 33.0 40.0 56-57 36.4264639348293 37.0 37.0 40.0 33.0 40.0 58-59 36.2532982418369 37.0 37.0 40.0 33.0 40.0 60-61 36.02624039096668 37.0 37.0 40.0 33.0 40.0 62-63 35.7830916725094 37.0 37.0 40.0 33.0 40.0 64-65 35.514208305061715 37.0 37.0 40.0 30.0 40.0 66-67 35.25957255104372 37.0 35.0 38.5 27.0 40.0 68-69 35.020739917082466 37.0 33.0 37.0 27.0 40.0 70-71 34.71796875421505 37.0 33.0 37.0 27.0 40.0 72-73 34.46430684574497 37.0 33.0 37.0 27.0 40.0 74-75 34.21042938354459 37.0 33.0 37.0 27.0 37.0 76-77 33.975301370577455 37.0 33.0 37.0 27.0 37.0 78-79 33.749272474045554 37.0 33.0 37.0 27.0 37.0 80-81 33.5160965197707 37.0 33.0 37.0 27.0 37.0 82-83 33.3156709874185 37.0 33.0 37.0 27.0 37.0 84-85 33.13749334904983 37.0 33.0 37.0 27.0 37.0 86-87 32.99637052735644 37.0 33.0 37.0 27.0 37.0 88-89 32.8479967248165 37.0 33.0 37.0 27.0 37.0 90-91 32.70692220360142 35.0 33.0 37.0 27.0 37.0 92-93 32.56915446672804 33.0 33.0 37.0 27.0 37.0 94-95 32.419074218566344 33.0 33.0 37.0 22.0 37.0 96-97 32.28861308516479 33.0 33.0 37.0 22.0 37.0 98-99 32.12554951428474 33.0 33.0 37.0 22.0 37.0 100-101 30.788852532216804 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.40946978424707936 2306 2 0.3759240033858404 2306 3 0.3928777197865969 2306 4 0.4532851185976625 2306 5 0.4846593521385074 2306 6 0.4441735662228723 2306 7 0.4812279278589884 2306 8 0.44862632825284976 2306 9 0.5564694436540165 2306 10-11 0.502354655931093 2306 12-13 0.5577578092893418 2306 14-15 0.785455802248272 2306 16-17 0.721398648220557 2306 18-19 0.7614250290349815 2306 20-21 0.7299400551293331 2306 22-23 0.7722362651416788 2306 24-25 0.8545331376921794 2306 26-27 0.8996401764524506 2306 28-29 0.7236522177983815 2306 30-31 0.7551581348949696 2306 32-33 0.7408022437920181 2306 34-35 0.7468246514213845 2306 36-37 0.6908696508366248 2306 38-39 0.627112445389173 2306 40-41 0.5518710461818372 2306 42-43 0.5419197097883597 2306 44-45 0.6704574748301084 2306 46-47 0.5980115776870818 2306 48-49 0.6229897326658005 2306 50-51 0.5979370757569029 2306 52-53 0.6991324266028229 2306 54-55 0.8286720341411993 2306 56-57 0.7226650471855791 2306 58-59 0.866827151790666 2306 60-61 0.8088448771386396 2306 62-63 0.8282768218408663 2306 64-65 0.8310013342126652 2306 66-67 0.8432073998906944 2306 68-69 0.7081674690607329 2306 70-71 0.6963156235408832 2306 72-73 0.7398120577811866 2306 74-75 0.8072402509434298 2306 76-77 0.7392673898871749 2306 78-79 0.6313832788129972 2306 80-81 0.7516721249899447 2306 82-83 0.5905509834855209 2306 84-85 0.6374430593883815 2306 86-87 0.6534668495595426 2306 88-89 0.6717175107479036 2306 90-91 0.676113764000327 2306 92-93 0.519762119234187 2306 94-95 0.5594996163662245 2306 96-97 0.5567269928847978 2306 98-99 0.6882626244662617 2306 100-101 0.4440856075646096 2307 1 0.2863220642330404 2307 2 0.26986982413117744 2307 3 0.27127050682805987 2307 4 0.3537031022445518 2307 5 0.296491872062397 2307 6 0.42169149664956507 2307 7 0.4260164832523827 2307 8 0.40711151877071927 2307 9 0.3434199023336504 2307 10-11 0.3441522857035366 2307 12-13 0.30044701502242077 2307 14-15 0.4973512800020501 2307 16-17 0.49613669649108516 2307 18-19 0.542841016771682 2307 20-21 0.49449528684538535 2307 22-23 0.5242242443606884 2307 24-25 0.5876426481828005 2307 26-27 0.5372196928652784 2307 28-29 0.460476668953703 2307 30-31 0.3725402527890225 2307 32-33 0.3678085806078357 2307 34-35 0.4198788839574803 2307 36-37 0.43022356141813844 2307 38-39 0.35914640587150615 2307 40-41 0.309345175596917 2307 42-43 0.3023744759961673 2307 44-45 0.33679659338373824 2307 46-47 0.25049023602387166 2307 48-49 0.39684833109466666 2307 50-51 0.36555592856479535 2307 52-53 0.42111512054876954 2307 54-55 0.3427129163102336 2307 56-57 0.4305957739749289 2307 58-59 0.2549482096536906 2307 60-61 0.37258821006980725 2307 62-63 0.33085239726501214 2307 64-65 0.4751853597044686 2307 66-67 0.4664068090179896 2307 68-69 0.3394171513625466 2307 70-71 0.3369689273877441 2307 72-73 0.38128656935275984 2307 74-75 0.4464064516527486 2307 76-77 0.4216090659495464 2307 78-79 0.4525418866120461 2307 80-81 0.3339526805131712 2307 82-83 0.31400981806749684 2307 84-85 0.4695910543836064 2307 86-87 0.4328593065011077 2307 88-89 0.46183625484943747 2307 90-91 0.33420926514893523 2307 92-93 0.39527868884086104 2307 94-95 0.5012674820284246 2307 96-97 0.5643722046907413 2307 98-99 0.3442183555579419 2307 100-101 0.5103157135334158 2201 1 0.3211596447741485 2201 2 0.27623764323436006 2201 3 0.33056793654657923 2201 4 0.4764449045481456 2201 5 0.39196754781880117 2201 6 0.3629865361986262 2201 7 0.38149510254476837 2201 8 0.4026800264925967 2201 9 0.3631387433465676 2201 10-11 0.387546982983892 2201 12-13 0.33975410756470836 2201 14-15 0.48910641520120635 2201 16-17 0.434569198600407 2201 18-19 0.4691785158582391 2201 20-21 0.46437306398094336 2201 22-23 0.5552385341904582 2201 24-25 0.6340892405040961 2201 26-27 0.6932008793154836 2201 28-29 0.5187617594195544 2201 30-31 0.44265745727801686 2201 32-33 0.3288490806760649 2201 34-35 0.3619746177341412 2201 36-37 0.4304548718171972 2201 38-39 0.43001961573821035 2201 40-41 0.4432416910408534 2201 42-43 0.43503976802470135 2201 44-45 0.5587552235929749 2201 46-47 0.5570687995349957 2201 48-49 0.5261176125378597 2201 50-51 0.5730007717508272 2201 52-53 0.5519927445794153 2201 54-55 0.6455450835723937 2201 56-57 0.6064084355801285 2201 58-59 0.6518382647087577 2201 60-61 0.577453013188638 2201 62-63 0.5486301119615931 2201 64-65 0.6187469219582553 2201 66-67 0.5809564725234893 2201 68-69 0.6170578035324681 2201 70-71 0.5867755512101169 2201 72-73 0.6130403798467228 2201 74-75 0.5543342422234687 2201 76-77 0.46768703226030794 2201 78-79 0.48150060334892686 2201 80-81 0.4514909108552345 2201 82-83 0.40467716737914117 2201 84-85 0.555925352289286 2201 86-87 0.64162133073728 2201 88-89 0.5766164877399049 2201 90-91 0.5761284779685312 2201 92-93 0.6237635441658398 2201 94-95 0.516862408612738 2201 96-97 0.6206313056263255 2201 98-99 0.5938113393173126 2201 100-101 0.36566480356241016 1101 1 0.2656563652044781 1101 2 0.18163515029161914 1101 3 0.27962717606224885 1101 4 0.2852806055000272 1101 5 0.35185551600552145 1101 6 0.34349289014896556 1101 7 0.40983191096456295 1101 8 0.31047209611039506 1101 9 0.35002046466812686 1101 10-11 0.33322854899740406 1101 12-13 0.29492480132461907 1101 14-15 0.5033107447582381 1101 16-17 0.5109160882253079 1101 18-19 0.48459325326466995 1101 20-21 0.48586609377273504 1101 22-23 0.5198873017437862 1101 24-25 0.573635862623334 1101 26-27 0.5341883842034036 1101 28-29 0.5150973740124272 1101 30-31 0.5857432787870707 1101 32-33 0.5785081353143724 1101 34-35 0.5876058329919118 1101 36-37 0.5517125905852893 1101 38-39 0.5063692688513441 1101 40-41 0.4434772784133685 1101 42-43 0.2895662926757012 1101 44-45 0.3038462321694553 1101 46-47 0.39234348335740066 1101 48-49 0.5325496314180143 1101 50-51 0.3647145904355966 1101 52-53 0.4793675827426398 1101 54-55 0.6642891334367746 1101 56-57 0.6895880498800793 1101 58-59 0.72201981206873 1101 60-61 0.6746960191453297 1101 62-63 0.5767755488207271 1101 64-65 0.7039984615666484 1101 66-67 0.5811975868724844 1101 68-69 0.492745739386379 1101 70-71 0.5054308931019733 1101 72-73 0.5410991813495087 1101 74-75 0.4660856996180769 1101 76-77 0.509107575755543 1101 78-79 0.667998752502676 1101 80-81 0.5652982653264971 1101 82-83 0.461169084866917 1101 84-85 0.5241383288010155 1101 86-87 0.5055879312184075 1101 88-89 0.6099496828988293 1101 90-91 0.4725418847553797 1101 92-93 0.42018151070828935 1101 94-95 0.3736149230416004 1101 96-97 0.5643977742890556 1101 98-99 0.5854345155877922 1101 100-101 0.6631460222054528 2304 1 0.33397196906187787 2304 2 0.3132784712587977 2304 3 0.2872587866800558 2304 4 0.2943537716373399 2304 5 0.37451096069008116 2304 6 0.33556041041185125 2304 7 0.3474512775173366 2304 8 0.3151473405992533 2304 9 0.32208280474964823 2304 10-11 0.3899796595968965 2304 12-13 0.3615431109110929 2304 14-15 0.5645644271346342 2304 16-17 0.5577574857231227 2304 18-19 0.5561844061428118 2304 20-21 0.5205013619125225 2304 22-23 0.5039674009521136 2304 24-25 0.613669769264952 2304 26-27 0.669133416723426 2304 28-29 0.6586341820788633 2304 30-31 0.6472363483323136 2304 32-33 0.5801531045210666 2304 34-35 0.5404498310454215 2304 36-37 0.5112463481004781 2304 38-39 0.5019504171772056 2304 40-41 0.5246759671472603 2304 42-43 0.48288058840649484 2304 44-45 0.4541938014086 2304 46-47 0.4986640894909442 2304 48-49 0.5491935802859587 2304 50-51 0.37174642500467314 2304 52-53 0.4598915397733023 2304 54-55 0.6588466726936488 2304 56-57 0.7047950216716359 2304 58-59 0.7729739596149443 2304 60-61 0.5878442612848005 2304 62-63 0.7478052537596867 2304 64-65 0.7683909937334761 2304 66-67 0.6955945666697758 2304 68-69 0.6253930475234455 2304 70-71 0.6856063652048618 2304 72-73 0.7877379500360959 2304 74-75 0.778427089267133 2304 76-77 0.8053609703440756 2304 78-79 0.746692378786598 2304 80-81 0.8222431094762896 2304 82-83 0.7660378012807669 2304 84-85 0.7266487545700642 2304 86-87 0.7045467932529093 2304 88-89 0.7802932754330882 2304 90-91 0.7462111275593699 2304 92-93 0.7563079123659548 2304 94-95 0.8622335675084827 2304 96-97 0.6268470843830798 2304 98-99 0.7366650702857953 2304 100-101 0.8325346823175295 2202 1 0.22892143315877433 2202 2 0.25813550315990597 2202 3 0.2020571739527739 2202 4 0.24590468352349149 2202 5 0.2820682199263942 2202 6 0.2840245523923528 2202 7 0.2701747619856718 2202 8 0.2307838718974793 2202 9 0.2288531903758937 2202 10-11 0.29499521525911376 2202 12-13 0.3780729110219241 2202 14-15 0.41306725827969615 2202 16-17 0.2964616314270998 2202 18-19 0.29073801172568636 2202 20-21 0.38975016669368756 2202 22-23 0.2717815067887912 2202 24-25 0.3933526126567415 2202 26-27 0.400865331357096 2202 28-29 0.3543055408975917 2202 30-31 0.3670682540936525 2202 32-33 0.37011548439921427 2202 34-35 0.30104880767338216 2202 36-37 0.3517322680080852 2202 38-39 0.2794886299996051 2202 40-41 0.2501925256733486 2202 42-43 0.27745657843458815 2202 44-45 0.2643040000760024 2202 46-47 0.24489207775619803 2202 48-49 0.2645956857910221 2202 50-51 0.37223513024123633 2202 52-53 0.3560338591741683 2202 54-55 0.49989402183136633 2202 56-57 0.5170414876491662 2202 58-59 0.4887175042696583 2202 60-61 0.545907424982147 2202 62-63 0.4638351491389514 2202 64-65 0.41230068653278096 2202 66-67 0.43959538263298015 2202 68-69 0.29843942652308186 2202 70-71 0.3886086661920203 2202 72-73 0.4000497948838202 2202 74-75 0.31318006767829587 2202 76-77 0.40930337439568376 2202 78-79 0.412460358703143 2202 80-81 0.4329470268269233 2202 82-83 0.39770677329802595 2202 84-85 0.351420946660312 2202 86-87 0.365410085235375 2202 88-89 0.3832913584286146 2202 90-91 0.3042257843530294 2202 92-93 0.36262330032663215 2202 94-95 0.4193402652428375 2202 96-97 0.37891353665941807 2202 98-99 0.3348475522393386 2202 100-101 0.4272144131223996 1102 1 0.1526079924748842 1102 2 0.1089937972142252 1102 3 0.20233670315282382 1102 4 0.1511704339581854 1102 5 0.1180585377721215 1102 6 0.13254839562664245 1102 7 0.18834217516621266 1102 8 0.178353046907489 1102 9 0.18401257951677508 1102 10-11 0.23254265015928866 1102 12-13 0.17507812070899575 1102 14-15 0.14560511089684525 1102 16-17 0.14477486291696806 1102 18-19 0.2218095686804915 1102 20-21 0.16811769411181388 1102 22-23 0.1676162775433383 1102 24-25 0.16067196307152187 1102 26-27 0.28277605875681644 1102 28-29 0.18947345689989703 1102 30-31 0.1540801964254186 1102 32-33 0.07836820400015654 1102 34-35 0.25472336038728116 1102 36-37 0.11438630049050147 1102 38-39 0.13043328747119176 1102 40-41 0.14431467796470088 1102 42-43 0.16328634617567417 1102 44-45 0.13597150635418842 1102 46-47 0.23975418298520168 1102 48-49 0.2025084619688613 1102 50-51 0.16323908665453501 1102 52-53 0.2551764315107121 1102 54-55 0.43751474552293246 1102 56-57 0.3671798502712349 1102 58-59 0.4105301782763888 1102 60-61 0.459981574493483 1102 62-63 0.5668601292355859 1102 64-65 0.5337538398332455 1102 66-67 0.45270454599239685 1102 68-69 0.46136040978536386 1102 70-71 0.3699576336273793 1102 72-73 0.4459595320775449 1102 74-75 0.4117174581152909 1102 76-77 0.3878497248895414 1102 78-79 0.39562567521777936 1102 80-81 0.5020455187712187 1102 82-83 0.41523098577235373 1102 84-85 0.46672233096389704 1102 86-87 0.4124368759314123 1102 88-89 0.30652392892842073 1102 90-91 0.3909201235441131 1102 92-93 0.35571737745810594 1102 94-95 0.299268020846263 1102 96-97 0.40298120556704475 1102 98-99 0.4499688847674008 1102 100-101 0.4997490112603806 2305 1 0.14333633043206362 2305 2 0.18765387205673534 2305 3 0.12628757526962886 2305 4 0.2878678388379754 2305 5 0.15826836872712846 2305 6 0.176046059155766 2305 7 0.24178157375273912 2305 8 0.203545827358397 2305 9 0.1893352999185538 2305 10-11 0.212090471349903 2305 12-13 0.19247362922621392 2305 14-15 0.12691513662768017 2305 16-17 0.22420106843706122 2305 18-19 0.19134315440512495 2305 20-21 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-0.6172781224365522 1213 68-69 -0.6482459523254462 1213 70-71 -0.6295155045015974 1213 72-73 -0.6995630536635105 1213 74-75 -0.6889059348958071 1213 76-77 -0.7773322222047909 1213 78-79 -0.743153835411313 1213 80-81 -0.7133417376046154 1213 82-83 -0.537879855782414 1213 84-85 -0.4944168328136058 1213 86-87 -0.7043590952179599 1213 88-89 -0.4837454844608544 1213 90-91 -0.49766192138862664 1213 92-93 -0.4665657319316949 1213 94-95 -0.49340985480814226 1213 96-97 -0.5803492838933124 1213 98-99 -0.6817338543639053 1213 100-101 -1.1861494610982035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1820.0 3 139.0 4 114.0 5 206.0 6 385.0 7 1294.0 8 4797.0 9 6824.0 10 8603.0 11 8183.0 12 7076.0 13 6627.0 14 6222.0 15 6597.0 16 7036.0 17 7335.0 18 8151.0 19 9199.0 20 10577.0 21 11949.0 22 13600.0 23 15273.0 24 17924.0 25 20841.0 26 25391.0 27 30604.0 28 37595.0 29 45668.0 30 57486.0 31 76673.0 32 105163.0 33 153084.0 34 232690.0 35 387278.0 36 753432.0 37 1361337.0 38 443161.0 39 1967.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.926684275973386 32.222046083404706 15.596691975755977 19.25457766486593 2 31.25372963033204 24.763767562875874 29.566926712554896 14.415576094237192 3 32.721315506915396 23.352953917437144 24.084236976046284 19.841493599601172 4 28.81668354076123 22.82018079974977 28.481825365468193 19.88131029402081 5 30.95204039158807 21.058251992593025 28.424067426332844 19.565640189486064 6 28.47816091007594 21.400916076418095 30.268758119750704 19.852164893755255 7 31.788653085437442 22.04293648227281 24.39152136572722 21.776889066562532 8 27.829450126130606 25.47457491530202 24.989704542888134 21.706270415679242 9 26.320807711261846 23.184095162353994 28.344998513345427 22.150098613038733 10-11 22.292130705117273 29.067895752950367 30.995685390738696 17.64428815119366 12-13 22.632406950195207 28.307046573490275 30.04975321474247 19.01079326157205 14-15 22.364583961693008 28.16377836654052 30.51991974659908 18.951717925167394 16-17 22.558477890733243 27.75975351711006 30.81329348061913 18.86847511153757 18-19 22.104070658250464 27.800633607320357 30.924430353993433 19.170865380435746 20-21 22.174806222733746 28.286150591699805 30.30963855034351 19.229404635222934 22-23 22.275156725327765 28.2625060335859 29.974160239951836 19.488177001134503 24-25 21.61560361430879 28.554014629517095 30.22388266666529 19.606499089508823 26-27 21.54076589910974 28.678214272139442 30.4788624962232 19.302157332527617 28-29 21.39588301349753 29.121977086128446 30.100144378745842 19.381995521628184 30-31 21.2524542913778 29.437965826144957 30.100288161583684 19.20929172089356 32-33 20.761510213233834 29.49935390907228 30.478426693568984 19.260709184124902 34-35 20.82538307749321 29.864593719975215 30.398961688966025 18.911061513565553 36-37 20.850825182492283 29.329026577306244 30.912734478366783 18.90741376183469 38-39 21.04009303582722 28.837370369707134 30.91769042449593 19.204846169969716 40-41 21.077937246892894 29.290417043089384 30.00188978909722 19.629755920920505 42-43 21.496061185606465 28.909588104597507 29.98851828966339 19.60583242013264 44-45 20.89918407771513 29.035192767694546 30.33284105232023 19.73278210227009 46-47 21.144864810814518 29.297811026357472 30.02381795474846 19.533506208079547 48-49 20.857229890940467 29.651884690379475 29.84953866564818 19.64134675303188 50-51 20.983748215174593 29.37250815436973 29.508567870655778 20.1351757597999 52-53 21.333488482134435 29.101649783281353 29.43323524424081 20.131626490343404 54-55 21.58966819256756 29.121774724258216 29.457604330445925 19.8309527527283 56-57 21.645078037322367 28.83766716531182 29.568072794461557 19.94918200290425 58-59 21.576518298645322 29.301179906453246 29.41140999291461 19.710891801986822 60-61 21.056881338106777 29.531273328799667 29.101124883722342 20.310720449371214 62-63 21.176966770503743 29.48154545227355 29.06002239912856 20.281465378094147 64-65 20.926366475062864 29.351054946034616 29.315794314960787 20.406784263941734 66-67 21.238148659362384 29.04200380252923 29.26934908419028 20.450498453918105 68-69 21.318450168291655 29.092162917812843 29.766115373600776 19.82327154029473 70-71 21.220016557511077 29.917410784431407 29.26940626331388 19.593166394743633 72-73 20.971100588393618 30.163859058544457 28.99495313069447 19.87008722236746 74-75 21.120720021297103 29.655556481429368 28.935787617312116 20.28793587996142 76-77 21.195010129725656 29.354529579816322 28.989393248042987 20.461067042415028 78-79 20.887436967452565 29.083368061654834 29.68174262166975 20.34745234922285 80-81 20.839883600792035 29.437706924269165 29.396518481864593 20.32589099307421 82-83 20.45249150959952 29.7541153220451 29.225557451903196 20.56783571645219 84-85 20.49320916728592 29.74336150568191 29.348473581513378 20.41495574551879 86-87 20.631875497727318 29.45929854800517 29.909441812302706 19.99938414196481 88-89 20.810477091316347 29.966412872346883 29.550655075905723 19.672454960431047 90-91 21.112185610657615 29.75516396366408 29.00597427186911 20.126676153809193 92-93 20.83561200272951 29.823375322673755 29.045686816930477 20.29532585766626 94-95 20.440469043098812 29.924652476482557 29.07645839337774 20.55842008704089 96-97 20.58163413272837 30.322962086107598 28.91102032910751 20.18438345205652 98-99 20.86421620011866 30.086170186182116 28.807090743138964 20.24252287056027 100-101 20.96561909737572 30.10320350522497 28.44173552739642 20.489441870002896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1603.0 1 951.0 2 265.0 3 230.0 4 209.0 5 219.0 6 291.0 7 434.5 8 625.0 9 853.0 10 1050.5 11 1247.0 12 1322.5 13 1214.5 14 1116.0 15 955.5 16 829.5 17 772.0 18 754.0 19 809.0 20 962.5 21 1304.5 22 1798.0 23 2451.5 24 3472.0 25 5154.5 26 8026.5 27 12286.0 28 17067.5 29 22280.0 30 28095.0 31 32668.5 32 40053.5 33 55396.0 34 77576.5 35 110519.5 36 153185.0 37 203529.5 38 254919.5 39 274817.5 40 281250.5 41 270826.0 42 238795.0 43 223683.5 44 210600.5 45 200898.5 46 205328.5 47 192344.5 48 157728.5 49 127991.5 50 101764.5 51 82125.5 52 68174.0 53 54339.5 54 44000.0 55 34251.0 56 23882.5 57 15335.5 58 10076.5 59 7345.5 60 5579.5 61 4122.0 62 2924.5 63 2011.5 64 1453.0 65 1106.0 66 851.5 67 629.0 68 485.0 69 380.5 70 271.0 71 192.0 72 166.0 73 145.0 74 112.5 75 85.5 76 79.5 77 67.5 78 54.0 79 40.5 80 38.0 81 38.5 82 29.0 83 25.5 84 24.5 85 22.5 86 21.5 87 19.0 88 15.0 89 12.0 90 12.0 91 9.5 92 5.5 93 5.5 94 5.0 95 3.5 96 2.0 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1664054244520144 2 0.1590832774752004 3 0.12555555184452588 4 0.12036581960131038 5 0.13244093917710886 6 0.15271172501818334 7 0.12907532074215225 8 0.11982629298196619 9 0.11245276251759563 10-11 0.09978673283489639 12-13 0.12883124917625846 14-15 0.12497748760951427 16-17 0.1152403167175406 18-19 0.1131464395996096 20-21 0.10632528162647235 22-23 0.12043004896075613 24-25 0.41354715372731965 26-27 0.30055486459037983 28-29 0.3853504649306413 30-31 0.21947172122608197 32-33 0.25967930023911306 34-35 0.2726536308471519 36-37 0.26958346746564565 38-39 0.2881457523454635 40-41 0.28043822921197514 42-43 0.3362535425703202 44-45 0.28386807700637745 46-47 0.309200136371776 48-49 0.309200136371776 50-51 0.3088019143432124 52-53 0.31228314562517134 54-55 0.29070208085140387 56-57 0.3028028921709806 58-59 0.30268727932397826 60-61 0.2817485081446682 62-63 0.28004000718341154 64-65 0.28779891380445655 66-67 0.2877475303168999 68-69 0.28498566786073326 70-71 0.25884431856631845 72-73 0.24910714767434483 74-75 0.2590370066446557 76-77 0.2547464854336805 78-79 0.25134232938305645 80-81 0.2606170488870208 82-83 0.30167245544473564 84-85 0.30726040971651475 86-87 0.2963928020982961 88-89 0.3030726554806527 90-91 0.28023269526174877 92-93 0.2642138930159821 94-95 0.2507000357885991 96-97 0.2502375844005898 98-99 0.2714075812739046 100-101 0.2711249720923433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3892301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.947221056111644 #Duplication Level Percentage of deduplicated Percentage of total 1 87.58360000076443 38.49055830123655 2 5.812215354572359 5.108614260262356 3 1.7549480179230168 2.3137526545694342 4 1.0151130686490915 1.7844559369947783 5 0.5453953042512466 1.1984303999447399 6 0.4592422122103053 1.2109451410982413 7 0.310939341085512 0.9565443970408686 8 0.2850600096490199 1.0022076206642239 9 0.2085841453034633 0.8250024188206272 >10 1.61311679578181 14.243027385013484 >50 0.22550424273065764 6.949530220178018 >100 0.15389316880437293 13.248588101687723 >500 0.02324336415453765 7.028132686750327 >1k 0.009086010748789352 5.508495900609813 >5k 5.896337136824405E-5 0.13171457512875037 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 5083 0.13059113362507163 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.47827544683723E-4 0.0 0.0 2 9.505945197969016E-4 0.0 0.0 3 0.001207511957579848 0.0 0.0 4 0.0015415046266976783 0.0 0.0 5 0.0017984220644806247 0.0 0.0 6 0.0028260918156124103 0.0 0.0 7 0.003494077153848071 0.0 0.0 8 0.004752972598984508 0.0 0.0 9 0.005266807474550401 0.0 0.0 10-11 0.0076304479021535076 0.0 0.0 12-13 0.010944682849553515 0.0 0.0 14-15 0.015710501320427173 0.0 0.0 16-17 0.023122569400465174 0.0 0.0 18-19 0.03266705221410163 0.0 0.0 20-21 0.042442760721742745 0.0 0.0 22-23 0.054993177557439675 0.0 1.284587188914732E-5 24-25 0.06826296321892886 0.0 2.569174377829464E-5 26-27 0.08330547920112036 0.0 2.569174377829464E-5 28-29 0.09896459703399095 0.0 2.569174377829464E-5 30-31 0.11893992782161503 0.0 2.569174377829464E-5 32-33 0.1373095246230957 0.0 2.569174377829464E-5 34-35 0.157477543489057 0.0 2.569174377829464E-5 36-37 0.18227007623511132 0.0 2.569174377829464E-5 38-39 0.20904087325209433 0.0 2.569174377829464E-5 40-41 0.2392543639353688 0.0 2.569174377829464E-5 42-43 0.27233248404992316 0.0 2.569174377829464E-5 44-45 0.31153808505560077 0.0 2.569174377829464E-5 46-47 0.351578667734073 0.0 2.569174377829464E-5 48-49 0.39581985052029633 0.0 2.569174377829464E-5 50-51 0.44376064441059415 0.0 2.569174377829464E-5 52-53 0.49460460534783923 0.0 2.569174377829464E-5 54-55 0.5492252526204937 0.0 2.569174377829464E-5 56-57 0.6076996614598922 0.0 2.569174377829464E-5 58-59 0.6709784263858319 0.0 2.569174377829464E-5 60-61 0.7351949399596793 0.0 2.569174377829464E-5 62-63 0.804048813285509 0.0 2.569174377829464E-5 64-65 0.8765123766121891 0.0 2.569174377829464E-5 66-67 0.9560411694779001 0.0 2.569174377829464E-5 68-69 1.0415047551563972 0.0 5.138348755658928E-5 70-71 1.1312074785583128 0.0 5.138348755658928E-5 72-73 1.225213569043093 0.0 5.138348755658928E-5 74-75 1.3285586083912833 0.0 5.138348755658928E-5 76-77 1.4369906130075756 0.0 5.138348755658928E-5 78-79 1.55187124531222 0.0 5.138348755658928E-5 80-81 1.6759880595051615 0.0 5.138348755658928E-5 82-83 1.8108311767255412 0.0 5.138348755658928E-5 84-85 1.9555399235567856 0.0 5.138348755658928E-5 86-87 2.1096004651233295 0.0 5.138348755658928E-5 88-89 2.277701544664711 0.0 5.138348755658928E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGA 25 0.005047926 56.926388 1 CGACGCC 80 4.278263E-9 47.438656 1 CGGGAGA 600 0.0 44.267536 4 GAGCGGC 400 0.0 43.868904 9 CGGACCG 45 9.6893334E-4 42.167694 1 GATCCGG 125 3.6379788E-12 41.746017 1 CGAGGCG 150 0.0 41.113503 1 GATTCGC 70 4.3609452E-6 40.648632 7 CGGCGAC 95 1.9525032E-8 39.948345 1 GTGTCGC 75 6.988228E-6 37.950928 1 CGCGCCG 50 0.001626583 37.950924 1 CGGAGCG 50 0.001626583 37.950924 1 CGGGCCA 120 3.8471626E-9 35.578995 1 CGGGTGC 135 3.1286618E-10 35.139748 1 GTGACCC 285 0.0 34.9548 1 CGAGGGG 390 0.0 34.058525 1 CGAGCGC 70 2.0698809E-4 33.884758 1 CACCGAG 170 3.6379788E-12 33.48611 1 CGAGTGG 245 0.0 32.91662 1 GCGGCCC 130 8.418283E-9 32.835808 3 >>END_MODULE