##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3096019 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.052616602159095 33.0 33.0 33.0 27.0 33.0 2 30.53460750725367 33.0 33.0 33.0 22.0 33.0 3 30.364413461286897 33.0 33.0 33.0 22.0 33.0 4 33.68803744421465 37.0 37.0 37.0 22.0 37.0 5 33.79148739074276 37.0 37.0 37.0 22.0 37.0 6 34.50930210699612 37.0 37.0 37.0 27.0 37.0 7 33.715833462262346 37.0 37.0 37.0 22.0 37.0 8 34.17531675354706 37.0 37.0 37.0 27.0 37.0 9 33.48539753793501 37.0 37.0 37.0 15.0 37.0 10-11 33.54358048190272 37.0 37.0 37.0 18.5 37.0 12-13 33.50269539689518 37.0 37.0 37.0 18.5 37.0 14-15 34.98324267389832 40.0 37.0 40.0 15.0 40.0 16-17 34.90602657800227 40.0 37.0 40.0 18.5 40.0 18-19 34.990349703926235 40.0 37.0 40.0 18.5 40.0 20-21 34.96939149921238 40.0 37.0 40.0 18.5 40.0 22-23 35.86248324057443 40.0 37.0 40.0 27.0 40.0 24-25 35.02200244895138 40.0 37.0 40.0 22.0 40.0 26-27 34.931219737346574 37.0 37.0 40.0 21.0 40.0 28-29 34.5287895520021 37.0 37.0 40.0 15.0 40.0 30-31 34.51118484737981 37.0 37.0 40.0 15.0 40.0 32-33 34.28046501006615 37.0 37.0 40.0 15.0 40.0 34-35 34.60365617911259 37.0 37.0 40.0 18.5 40.0 36-37 34.26197352148033 37.0 37.0 40.0 15.0 40.0 38-39 34.21171591647209 37.0 37.0 40.0 15.0 40.0 40-41 34.012575504220095 37.0 35.0 40.0 15.0 40.0 42-43 33.878508497525374 37.0 33.0 40.0 15.0 40.0 44-45 33.73447966566096 37.0 33.0 40.0 15.0 40.0 46-47 33.641959884613115 37.0 33.0 40.0 15.0 40.0 48-49 33.52820153881484 37.0 33.0 40.0 15.0 40.0 50-51 32.6170532222186 37.0 33.0 38.5 15.0 38.5 52-53 33.07355268168574 37.0 33.0 38.5 15.0 40.0 54-55 33.617601506967496 37.0 33.0 40.0 15.0 40.0 56-57 33.965523467394746 37.0 33.0 40.0 18.5 40.0 58-59 33.315011148187395 37.0 33.0 40.0 15.0 40.0 60-61 33.1718726532363 37.0 33.0 40.0 10.5 40.0 62-63 32.755513935799485 37.0 33.0 40.0 6.0 40.0 64-65 32.53154551054112 37.0 33.0 38.5 6.0 40.0 66-67 32.417704316414074 37.0 33.0 37.0 10.5 40.0 68-69 32.561178565118624 37.0 33.0 37.0 15.0 40.0 70-71 32.110516440629084 37.0 33.0 37.0 15.0 40.0 72-73 32.49216881420947 37.0 33.0 37.0 18.5 37.0 74-75 32.60299210050067 37.0 33.0 37.0 22.0 37.0 76-77 32.26083980750764 37.0 33.0 37.0 22.0 37.0 78-79 31.739522593369095 37.0 33.0 37.0 18.5 37.0 80-81 31.164658873217512 35.0 33.0 37.0 15.0 37.0 82-83 30.784108075564134 33.0 33.0 37.0 15.0 37.0 84-85 30.522492917517624 33.0 27.0 37.0 15.0 37.0 86-87 30.369817013396883 33.0 27.0 37.0 15.0 37.0 88-89 30.23292315066542 33.0 27.0 37.0 6.0 37.0 90-91 30.09873663566018 33.0 27.0 37.0 6.0 37.0 92-93 29.94076828985869 33.0 27.0 37.0 6.0 37.0 94-95 29.792711381939192 33.0 27.0 37.0 6.0 37.0 96-97 29.671089712304735 33.0 27.0 37.0 6.0 37.0 98-99 29.516457424841384 33.0 27.0 37.0 6.0 37.0 100-101 28.31413437708231 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.026671995703683393 2306 2 -0.1365877316575208 2306 3 -0.09412442774406315 2306 4 0.08094414495528923 2306 5 -0.05433447700958993 2306 6 -0.14192900894324367 2306 7 0.4249977130070661 2306 8 -0.26238981297909447 2306 9 0.04411785179097194 2306 10-11 0.11414026057830995 2306 12-13 0.013667654593405132 2306 14-15 0.4822149088711356 2306 16-17 0.42096139030575586 2306 18-19 0.34201108891232224 2306 20-21 0.3151865684349815 2306 22-23 0.3423342695009879 2306 24-25 0.4572065034933601 2306 26-27 0.20546855713349288 2306 28-29 0.27118693885105927 2306 30-31 0.37207436530506044 2306 32-33 0.348941333094551 2306 34-35 0.17792720454876587 2306 36-37 0.476969212680288 2306 38-39 0.276273487005561 2306 40-41 0.30527057877399244 2306 42-43 0.22508254954053797 2306 44-45 0.3302469746322174 2306 46-47 0.14310105462122635 2306 48-49 0.3792324633751747 2306 50-51 0.2332419953066136 2306 52-53 0.3440662951368836 2306 54-55 0.4680614232788187 2306 56-57 0.23090189301879604 2306 58-59 0.5990633116030111 2306 60-61 0.46889785527945804 2306 62-63 0.4554609263376861 2306 64-65 0.4530851753593126 2306 66-67 0.3631256713784339 2306 68-69 0.31936546831747137 2306 70-71 0.4639986073100104 2306 72-73 0.08468644284616289 2306 74-75 0.3074282378074855 2306 76-77 0.48206575403441576 2306 78-79 0.31112189193085626 2306 80-81 0.45304558633049297 2306 82-83 0.46065045483574707 2306 84-85 0.48580788638524197 2306 86-87 0.46223341777990967 2306 88-89 0.5164150857482461 2306 90-91 0.43137157651812075 2306 92-93 0.23085924814977332 2306 94-95 0.3483503214331236 2306 96-97 0.4530087948262498 2306 98-99 0.5099943834310956 2306 100-101 0.4563812504667837 2307 1 -0.1461961761260433 2307 2 -0.27263635914772166 2307 3 -0.294170543851088 2307 4 -0.20627419258683233 2307 5 -0.1922063575744275 2307 6 -0.35957814315500514 2307 7 0.3089334727143651 2307 8 -0.2273765754339152 2307 9 -0.14966369676567837 2307 10-11 -0.12368453155194459 2307 12-13 -0.13224384693037194 2307 14-15 0.20805631946839753 2307 16-17 0.07918811486758415 2307 18-19 0.151267230549891 2307 20-21 -0.08745482882749656 2307 22-23 -0.035756551449061647 2307 24-25 0.24787021428424794 2307 26-27 -0.07615375750615527 2307 28-29 0.02464259099115651 2307 30-31 0.07774178483106908 2307 32-33 0.07946741786484779 2307 34-35 -0.18110294038360308 2307 36-37 0.30600069109044625 2307 38-39 -0.07136486826070154 2307 40-41 0.1485927720115967 2307 42-43 0.10185725568925363 2307 44-45 0.17852873293381322 2307 46-47 0.056223309532171584 2307 48-49 0.1287015725286622 2307 50-51 0.06643600799132088 2307 52-53 -0.03687441788480328 2307 54-55 0.17223965369822025 2307 56-57 0.23260721665108264 2307 58-59 0.2541027464306822 2307 60-61 0.07101377894895933 2307 62-63 0.33713227406702373 2307 64-65 0.3905739588664119 2307 66-67 0.34173488182067757 2307 68-69 0.18939319354325335 2307 70-71 0.28489948268261855 2307 72-73 -0.09049656309058918 2307 74-75 0.292948613625974 2307 76-77 0.3438502251298914 2307 78-79 0.37541302347010586 2307 80-81 0.5133065590469776 2307 82-83 0.485676638227833 2307 84-85 0.4813169832040636 2307 86-87 0.35632666677077296 2307 88-89 0.3838911456547365 2307 90-91 0.5519646407667445 2307 92-93 0.4165767286546078 2307 94-95 0.4663406037137001 2307 96-97 0.4224747925315562 2307 98-99 0.28843524230191875 2307 100-101 0.2923304227382246 2201 1 0.19573045900374098 2201 2 0.16520138743135604 2201 3 0.24729521269411592 2201 4 0.37188660177362465 2201 5 0.3104899081724142 2201 6 0.24737103655149895 2201 7 0.5439782218325391 2201 8 0.24260591869393267 2201 9 0.3573793136546186 2201 10-11 0.39476844584926596 2201 12-13 0.34381814506762254 2201 14-15 0.545953298766193 2201 16-17 0.4221851477625478 2201 18-19 0.49205070512071813 2201 20-21 0.46145139895428855 2201 22-23 0.3057217364661824 2201 24-25 0.6387034338761808 2201 26-27 0.4010468770995459 2201 28-29 0.4697679118251443 2201 30-31 0.6674873195496218 2201 32-33 0.3631114118669174 2201 34-35 0.362841863568228 2201 36-37 0.6318059683428459 2201 38-39 0.42679846566392854 2201 40-41 0.3529426792509156 2201 42-43 0.49878531040688046 2201 44-45 0.5216364556343294 2201 46-47 0.48312083716658094 2201 48-49 0.555486508062252 2201 50-51 0.5594761040952747 2201 52-53 0.5677759568911398 2201 54-55 0.6847641279156989 2201 56-57 0.7017223830329513 2201 58-59 0.8312503307772587 2201 60-61 0.587461499357218 2201 62-63 0.7069246813408299 2201 64-65 0.6380624535468797 2201 66-67 0.590460343995197 2201 68-69 0.48593807843774783 2201 70-71 0.5827493421267818 2201 72-73 0.4404330933596441 2201 74-75 0.38479511456785076 2201 76-77 0.44248328331206466 2201 78-79 0.5906224209989297 2201 80-81 0.6635850312946303 2201 82-83 0.5856965885493928 2201 84-85 0.6906270064453537 2201 86-87 0.6024998431710884 2201 88-89 0.6144951053774399 2201 90-91 0.5701093274420721 2201 92-93 0.6224672572691716 2201 94-95 0.5956643425038344 2201 96-97 0.6914518522774209 2201 98-99 0.6943794632002458 2201 100-101 0.710258271532382 1101 1 0.20452275852266055 1101 2 0.07784827095490598 1101 3 0.1063783232908051 1101 4 0.17853845776041055 1101 5 0.2792314374099689 1101 6 0.18740909817561402 1101 7 0.3628943342381987 1101 8 0.1840676716682026 1101 9 0.21510629791438163 1101 10-11 0.34944137085933846 1101 12-13 0.3242524433205105 1101 14-15 0.4510333029809601 1101 16-17 0.5250357880617003 1101 18-19 0.44254766992337125 1101 20-21 0.37839670427172933 1101 22-23 0.3215755852991151 1101 24-25 0.6320259594885442 1101 26-27 0.45687367579683524 1101 28-29 0.399226820634091 1101 30-31 0.4712302604133427 1101 32-33 0.53707523401971 1101 34-35 0.41580056767127616 1101 36-37 0.6104355166144302 1101 38-39 0.4694872877564009 1101 40-41 0.5143969196391964 1101 42-43 0.45958795059020474 1101 44-45 0.3408171976751291 1101 46-47 0.382324092835006 1101 48-49 0.4017674562567777 1101 50-51 0.289319409568769 1101 52-53 0.31960322854351375 1101 54-55 0.6737960741017801 1101 56-57 0.8054189936018972 1101 58-59 0.7896757281439974 1101 60-61 0.5554191940067881 1101 62-63 0.6450906290567673 1101 64-65 0.7090493531734268 1101 66-67 0.639245702013362 1101 68-69 0.535892351402012 1101 70-71 0.6482413561835187 1101 72-73 0.49757801307390537 1101 74-75 0.5416237656725755 1101 76-77 0.6560432195113961 1101 78-79 0.7364267141120457 1101 80-81 0.7003657353703296 1101 82-83 0.6128080555386575 1101 84-85 0.6756941330903459 1101 86-87 0.5634205210412127 1101 88-89 0.619633867023353 1101 90-91 0.6525195403764279 1101 92-93 0.5838395750789047 1101 94-95 0.48924441951185926 1101 96-97 0.5282877746645234 1101 98-99 0.47695565280697494 1101 100-101 0.6777263209224067 2304 1 -0.05280763335622041 2304 2 -0.2562100525236346 2304 3 -0.21734187892266377 2304 4 -0.37999287433886764 2304 5 -0.39544700429537016 2304 6 0.0022453856420838747 2304 7 -0.3061351778937649 2304 8 -0.18765095070452276 2304 9 -0.3705043958247316 2304 10-11 -0.26833091538383513 2304 12-13 -0.32903513917737826 2304 14-15 -0.3112387016417628 2304 16-17 -0.21718449521849692 2304 18-19 -0.20532918856024196 2304 20-21 -0.302344453115289 2304 22-23 0.11493495219485084 2304 24-25 -0.012707354019937611 2304 26-27 0.0442011784727967 2304 28-29 -0.14741939877474408 2304 30-31 -0.28857477986260704 2304 32-33 -0.24807737506115046 2304 34-35 -0.17316056409712388 2304 36-37 -0.3295215064922701 2304 38-39 -0.18767632181085503 2304 40-41 -0.2447645814246613 2304 42-43 -0.18808201483925302 2304 44-45 -0.2021018731387656 2304 46-47 -0.22469513342723246 2304 48-49 -0.1725834143845404 2304 50-51 -0.16312112164499126 2304 52-53 -0.08603010240776143 2304 54-55 0.019392383298736604 2304 56-57 0.1376782888054322 2304 58-59 -0.13624119036742144 2304 60-61 -0.15456937981041818 2304 62-63 -0.04180756572929312 2304 64-65 -0.018404298556220056 2304 66-67 0.007591602610951043 2304 68-69 0.1832251791248325 2304 70-71 -0.10991545530444569 2304 72-73 0.19553336413126488 2304 74-75 0.2390541039936238 2304 76-77 0.29075691563947714 2304 78-79 0.2352996560352274 2304 80-81 -0.04346915897781756 2304 82-83 -0.07386807019392094 2304 84-85 -0.06363541046938437 2304 86-87 0.02113480807276602 2304 88-89 0.10936912843858693 2304 90-91 0.032353864568047896 2304 92-93 -0.025734021602197288 2304 94-95 -0.020504335095687054 2304 96-97 -0.1042100334601308 2304 98-99 0.059345384764252884 2304 100-101 0.06383448007753145 2202 1 0.2591924720180714 2202 2 0.09681003546389988 2202 3 0.11429533360309918 2202 4 0.18443843884771383 2202 5 0.0855673853747092 2202 6 0.19417249905522027 2202 7 0.07218183255652377 2202 8 0.17242921481417284 2202 9 0.14961702778195018 2202 10-11 0.08241653283585038 2202 12-13 0.17147653406505015 2202 14-15 0.23731756924311753 2202 16-17 0.3329640420039581 2202 18-19 0.272133884843079 2202 20-21 0.28946137854116927 2202 22-23 0.2772022421909739 2202 24-25 0.3523937951677709 2202 26-27 0.40526720154571905 2202 28-29 0.39961401095494864 2202 30-31 0.3576882830032915 2202 32-33 0.3146421124955694 2202 34-35 0.35254215070937533 2202 36-37 0.32906578901574335 2202 38-39 0.3688134173675124 2202 40-41 0.3141680175309176 2202 42-43 0.3952168692064788 2202 44-45 0.3443084097709388 2202 46-47 0.3417950313011531 2202 48-49 0.34127786441354147 2202 50-51 0.4021303885509795 2202 52-53 0.42187752892001384 2202 54-55 0.6013786481651238 2202 56-57 0.7238086003283115 2202 58-59 0.516590541074045 2202 60-61 0.4860409602445728 2202 62-63 0.5679802874518955 2202 64-65 0.6158263373979054 2202 66-67 0.6001360675223211 2202 68-69 0.5459852965766387 2202 70-71 0.5861303744066504 2202 72-73 0.6499361857305672 2202 74-75 0.6050311769795087 2202 76-77 0.5943868573391242 2202 78-79 0.6754906266742999 2202 80-81 0.5007024268444731 2202 82-83 0.44798797636530097 2202 84-85 0.6255251211844453 2202 86-87 0.6470945753576949 2202 88-89 0.5034000801102891 2202 90-91 0.5736605868399174 2202 92-93 0.5369773602397814 2202 94-95 0.49631198332921045 2202 96-97 0.475453437528504 2202 98-99 0.49068035545196054 2202 100-101 0.5878856555074883 1102 1 0.00790161228354691 1102 2 -0.1215654342896535 1102 3 -0.006724657516322452 1102 4 -0.10385620344723634 1102 5 -0.010257024446922003 1102 6 0.11143372578015232 1102 7 -0.0058654700284108685 1102 8 0.11598873582977376 1102 9 0.025816471778000505 1102 10-11 0.0428106556250043 1102 12-13 0.046569721558419985 1102 14-15 -0.02303792928918824 1102 16-17 -0.06076609900865293 1102 18-19 0.033386845377378904 1102 20-21 0.048187066997428474 1102 22-23 0.0685406231177339 1102 24-25 0.024397941619390906 1102 26-27 0.2935220747575684 1102 28-29 0.08159300121275237 1102 30-31 0.07398378064699074 1102 32-33 0.07648602143390804 1102 34-35 0.028748354631822792 1102 36-37 0.19426065888579558 1102 38-39 0.08816321602800059 1102 40-41 0.14008757876807465 1102 42-43 0.062415811932183374 1102 44-45 0.040519430140939505 1102 46-47 0.08840028076560458 1102 48-49 0.07252758672503035 1102 50-51 0.1775175919558052 1102 52-53 0.24946219904241929 1102 54-55 0.4012093229120879 1102 56-57 0.4733326115596057 1102 58-59 0.3770086234410215 1102 60-61 0.26053274597998666 1102 62-63 0.33731285920319465 1102 64-65 0.3992701873504245 1102 66-67 0.4253422248339902 1102 68-69 0.2725793240181247 1102 70-71 0.40985275513278907 1102 72-73 0.48719886256874645 1102 74-75 0.5085530577436757 1102 76-77 0.4371807485462895 1102 78-79 0.3302371307974461 1102 80-81 0.35855105573787327 1102 82-83 0.3258800595919915 1102 84-85 0.3651854321574106 1102 86-87 0.4292658282800659 1102 88-89 0.4664345947135011 1102 90-91 0.41747528144797386 1102 92-93 0.3954990515624637 1102 94-95 0.48703364363255375 1102 96-97 0.5034642010758184 1102 98-99 0.3764981797969078 1102 100-101 0.4379007426819186 2305 1 0.2092381427235317 2305 2 0.202460227723666 2305 3 0.2337715982233881 2305 4 0.06107733237060842 2305 5 0.2056136327564957 2305 6 0.2895107403159969 2305 7 0.11694169835570989 2305 8 0.4304326583956595 2305 9 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-0.8890480249868524 1213 80-81 -0.9131964060397664 1213 82-83 -0.8852114782287899 1213 84-85 -0.8396557567042713 1213 86-87 -0.932462814430437 1213 88-89 -0.753060578963197 1213 90-91 -0.8302498556690985 1213 92-93 -0.6887386033452003 1213 94-95 -0.8028831691913574 1213 96-97 -0.7760830464477486 1213 98-99 -0.9401502819446783 1213 100-101 -1.2667871572582996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1466.0 3 169.0 4 258.0 5 259.0 6 525.0 7 3308.0 8 15989.0 9 55904.0 10 81725.0 11 58642.0 12 35878.0 13 24176.0 14 17852.0 15 15039.0 16 13697.0 17 12448.0 18 11863.0 19 11811.0 20 12280.0 21 12851.0 22 13680.0 23 14530.0 24 15996.0 25 18057.0 26 21214.0 27 26291.0 28 32434.0 29 39882.0 30 46552.0 31 59620.0 32 81722.0 33 116722.0 34 176420.0 35 293285.0 36 565532.0 37 939174.0 38 248411.0 39 357.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.498989448659884 22.857904543258943 23.48455313960038 13.158552868480793 2 30.06431918777049 26.676287693949103 31.65097992783259 11.608413190447815 3 36.05950973001485 19.405116144745392 30.030920403488466 14.504453721751295 4 27.28977245533768 17.285232169028003 35.437678426137566 19.98731694949675 5 34.41381692528715 16.038847084560455 32.965072165561956 16.58226382459043 6 30.647275446932287 16.422594277610425 38.0400970984338 14.890033177023486 7 35.66305264628568 19.471079212634283 28.529006168112105 16.336861972967938 8 27.278489383795435 33.05538497060926 23.99559421932082 15.670531426274481 9 33.146517032544 26.978798203795545 22.99075737885046 16.88392738481 10-11 27.058100615737583 29.908619032900123 26.72299180439177 16.310288546970522 12-13 29.16036617480382 23.695525304570264 26.73345794440884 20.410650576217073 14-15 25.02667648725762 24.26324142564581 29.50302258062169 21.207059506474877 16-17 26.904036135744132 25.571840195986965 30.31458385510132 17.209539813167584 18-19 26.434214949177594 25.730832280646837 30.74460033671955 17.09035243345601 20-21 25.13473292284959 28.749140110114407 28.983773847545464 17.13235311949054 22-23 28.903142019867147 25.662750109787343 28.259067076021992 17.175040794323525 24-25 24.45008800472991 30.30014039892234 28.04928200010573 17.20048959624202 26-27 22.613918921698797 31.966365124638973 27.972997116321125 17.44671883734111 28-29 23.855702874425614 30.24090158629697 27.758997251767415 18.144398287510004 30-31 26.681985337258112 26.739604341948077 28.436937213250363 18.141473107543444 32-33 22.330559882747274 29.55045295051328 30.515776965285802 17.603210201453646 34-35 27.595944324827116 26.891600143022597 28.41897199918125 17.09348353296904 36-37 23.71924147291805 27.342722998612896 30.23252424684743 18.70551128162162 38-39 20.350120005837116 30.207847615666466 31.661927384928745 17.780104993567676 40-41 22.02691657210081 31.30306221718303 28.481844245615463 18.188176965100702 42-43 24.764052612398782 27.290433592264442 30.36077568822748 17.5847381071093 44-45 21.850116371590335 29.557185490209353 31.077911391103285 17.514786747097027 46-47 23.891520597906432 30.25822427517485 28.218602087864486 17.631653039054232 48-49 20.569801418995777 29.617171151340866 30.32726841530784 19.485759014355512 50-51 19.63684797376708 27.27861802197859 31.952175009683287 21.13235899457104 52-53 25.851848167853937 26.739785865281203 27.714652713506567 19.6937132533583 54-55 27.50833351014525 26.8427054717809 27.47712283223864 18.171838185835213 56-57 27.959351973236085 26.82086834990257 26.929220539713448 18.29055913714789 58-59 25.899589282633645 26.623005842051594 27.448636031195406 20.028768844119355 60-61 21.284219520584994 26.858635216137095 27.236613583317194 24.620531679960717 62-63 24.73019022475808 26.8904498303294 27.543722932786345 20.835637012126178 64-65 21.444114946140257 28.755986440721887 30.181378828933152 19.618519784204704 66-67 20.08592634246346 28.52526529883755 28.59260833272058 22.796200025978408 68-69 19.895166024916897 27.065725703596634 34.22778270393269 18.81132556755378 70-71 19.27452287454496 32.35321119262784 30.183282300597092 18.18898363223011 72-73 19.159841954313755 36.0556575968216 26.485426267607515 18.299074181257126 74-75 19.84471462876408 35.56469902671794 26.10497897302073 18.485607371497252 76-77 20.143689787562614 34.932721711052835 26.374947966933927 18.548640534450627 78-79 19.93615536940726 34.78790853868911 26.93949072274408 18.33644536915955 80-81 19.15827222457991 34.934992829514066 26.87590744252237 19.03082750338366 82-83 18.80574859471345 34.08162081835932 27.116304369306487 19.996326217620744 84-85 18.989635287098643 32.96303657917659 27.74058719063844 20.306740943086332 86-87 19.208192238553384 32.701037587228235 28.400261364818054 19.690508809400324 88-89 19.70171960982415 32.98718242164385 27.72686783236052 19.58423013617148 90-91 20.119016096890665 32.318481828141664 27.39138708248614 20.17111499248153 92-93 19.71706441579908 32.78319790043383 27.437047342574637 20.062690341192457 94-95 19.42517454620577 33.09168427617319 27.54355641010862 19.939584767512418 96-97 20.030596016818905 33.40269704098729 26.82244871427274 19.744258227921062 98-99 20.648520011730504 32.3192536523823 26.509691024950317 20.52253531093688 100-101 20.64026598563876 32.36783668543963 26.027148814216993 20.964748514704624 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1692.0 1 1055.0 2 373.5 3 390.5 4 419.5 5 390.0 6 452.0 7 606.5 8 805.5 9 993.5 10 1173.5 11 1311.0 12 1335.5 13 1296.5 14 1224.0 15 1176.0 16 1118.0 17 1073.0 18 1039.5 19 1062.5 20 1177.0 21 1319.0 22 1509.0 23 1848.5 24 2355.5 25 3059.0 26 4519.5 27 6981.5 28 9418.0 29 11476.5 30 13973.0 31 16387.0 32 19577.5 33 28597.0 34 45866.5 35 75835.5 36 114322.0 37 154862.0 38 199814.0 39 230152.0 40 235136.0 41 215637.0 42 191277.0 43 185586.0 44 179109.0 45 167248.0 46 163893.0 47 150791.5 48 122944.0 49 101165.0 50 84880.5 51 72544.5 52 62298.0 53 50367.5 54 39488.5 55 30040.5 56 22030.0 57 15756.0 58 11525.0 59 8517.5 60 6284.0 61 4590.0 62 3394.5 63 2518.0 64 1838.0 65 1352.5 66 983.5 67 762.0 68 596.5 69 432.5 70 325.5 71 268.5 72 213.0 73 156.5 74 132.0 75 117.0 76 103.5 77 84.0 78 57.5 79 48.0 80 47.0 81 41.0 82 36.5 83 27.5 84 21.0 85 25.5 86 26.5 87 19.5 88 18.0 89 18.5 90 14.0 91 8.5 92 6.5 93 5.0 94 6.0 95 5.0 96 4.0 97 3.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.165761256633115 2 0.15739567489734396 3 0.12551602557994637 4 0.11960520914115838 5 0.13246042740693775 6 0.1525507433901407 7 0.12877827946146325 8 0.1200897022918787 9 0.11298380274798055 10-11 0.10151746484759945 12-13 0.12914972421034884 14-15 0.1252091799178235 16-17 0.1152609205563661 18-19 0.11416273608140001 20-21 0.10744443105807813 22-23 0.11876542101324314 24-25 0.40897681829471977 26-27 0.29597686577504856 28-29 0.3829110867859661 30-31 0.21836106302965194 32-33 0.2580249023019562 34-35 0.27115466668647703 36-37 0.2680216109784856 38-39 0.28673919636798095 40-41 0.27985939362775225 42-43 0.3333474374672765 44-45 0.2813451726232946 46-47 0.30736245481697627 48-49 0.3058766758214339 50-51 0.3073463050452856 52-53 0.3099948676025567 54-55 0.2885802703407182 56-57 0.3012093918028281 58-59 0.30188768221383655 60-61 0.2801339397464938 62-63 0.2766778886046888 64-65 0.28533416623089203 66-67 0.2855279634911801 68-69 0.282830951618837 70-71 0.25737891143432906 72-73 0.24778594705006657 74-75 0.25718511417404094 76-77 0.25317997079475285 78-79 0.25001461554338006 80-81 0.25883239088649007 82-83 0.2998043616657391 84-85 0.30492383929168393 86-87 0.2954116237658748 88-89 0.30230757627779414 90-91 0.278470513262354 92-93 0.2630151817543755 94-95 0.24951397262096905 96-97 0.2489810301551767 98-99 0.2694589406589559 100-101 0.2691682447685237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3096019.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.752535152770434 #Duplication Level Percentage of deduplicated Percentage of total 1 89.2856805473111 42.63617598974505 2 4.786222368590084 4.571085038101484 3 1.6016013097369108 2.2944156853180497 4 0.8650088268923498 1.652254576545346 5 0.5966557312992526 1.4245911891484755 6 0.4251760179010417 1.2181939644560669 7 0.33209826446730345 1.1100973833704273 8 0.21898420731617385 0.8365640846213727 9 0.23598266627230285 1.0141893509951372 >10 1.332902037636402 12.722672264545151 >50 0.1562657180619715 5.254823321054778 >100 0.13974005282350427 14.375426049479973 >500 0.01868488395041665 6.049896880948175 >1k 0.004725306833909534 3.702037443534784 >5k 2.0404568418973566E-4 0.5351239328817806 >10k+ 6.801522289230156E-5 0.6024528452541303 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 18549 0.599124230180758 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5548 0.17919786667975876 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5532 0.17868107398565705 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5396 0.17428833608579275 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4770 0.1540688219290644 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4628 0.14948228676891193 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3810 0.12306126028296337 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3684 0.11899151781691263 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3256 0.10516731324969258 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004651134246915151 0.0 0.0 2 0.005038728767491414 0.0 0.0 3 0.005813917808643939 0.0 0.0 4 0.006330710502745623 0.0 0.0 5 0.007622692237999831 0.0 0.0 6 0.00891467397325404 0.0 0.0 7 0.010400452968796381 0.0 0.0 8 0.012047729681245496 0.0 0.0 9 0.013662706850313257 0.0 0.0 10-11 0.02262583013863933 0.0 0.0 12-13 0.03129825753653321 0.0 0.0 14-15 0.04148876347335077 0.0 0.0 16-17 0.05327809680754543 0.0 0.0 18-19 0.06485748310976128 0.0 0.0 20-21 0.0742243506903543 0.0 0.0 22-23 0.08305827580515494 0.0 0.0 24-25 0.09248974247251066 0.0 0.0 26-27 0.10418217717656125 0.0 0.0 28-29 0.11816787946068806 0.0 0.0 30-31 0.21396832512978764 0.0 0.0 32-33 0.23061873974287628 0.0 0.0 34-35 0.24402305024613868 0.0 0.0 36-37 0.25734661189094765 0.0 0.0 38-39 0.2717037589239601 0.0 0.0 40-41 0.28727213883377334 0.0 0.0 42-43 0.3054244822140949 0.0 0.0 44-45 0.3222686940874717 3.2299543381355215E-5 0.0 46-47 0.3408732310751323 3.2299543381355215E-5 0.0 48-49 0.3617871854145598 3.2299543381355215E-5 0.0 50-51 0.3838316237723347 3.2299543381355215E-5 0.0 52-53 0.4072164931804359 3.2299543381355215E-5 0.0 54-55 0.432959229255376 3.2299543381355215E-5 0.0 56-57 0.46207726761366774 3.2299543381355215E-5 0.0 58-59 0.4933916749218916 3.2299543381355215E-5 0.0 60-61 0.5269670502668105 3.2299543381355215E-5 0.0 62-63 0.5649997625983562 3.2299543381355215E-5 0.0 64-65 0.6057779361173171 3.2299543381355215E-5 0.0 66-67 0.6469275543851636 3.2299543381355215E-5 0.0 68-69 0.6924537607811838 3.2299543381355215E-5 0.0 70-71 0.7404993315609498 3.2299543381355215E-5 0.0 72-73 0.7932929352177749 3.2299543381355215E-5 0.0 74-75 0.8503339288292482 3.2299543381355215E-5 0.0 76-77 0.9126235982401917 3.2299543381355215E-5 0.0 78-79 0.9825198101174444 3.2299543381355215E-5 0.0 80-81 1.0569379580680867 3.2299543381355215E-5 0.0 82-83 1.1393179434622334 3.2299543381355215E-5 0.0 84-85 1.2280770886741976 3.2299543381355215E-5 0.0 86-87 1.3269298411928352 3.2299543381355215E-5 0.0 88-89 1.4306436749903666 3.2299543381355215E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 4415 0.0 88.400665 4 GAGCGGC 3600 0.0 87.33259 9 AGAGCGG 3735 0.0 87.22167 8 ATTCGGA 655 0.0 84.71064 2 AAGCGGC 1275 0.0 84.426926 9 GAGAGCG 4195 0.0 83.08328 7 TCGGGAG 4795 0.0 81.79058 3 CGGAAAG 1775 0.0 80.95257 5 GTTCGGA 530 0.0 80.53043 2 TCGGAGA 965 0.0 78.1369 4 GATCCGG 995 0.0 76.27242 1 TCGGGAA 1495 0.0 76.1301 3 GATCGGG 6465 0.0 75.86171 1 AAGGGGC 2860 0.0 75.77317 9 ATCGGGA 6325 0.0 75.652626 2 AAAGCGG 1520 0.0 74.56115 8 ATCCGGA 970 0.0 74.31307 2 GAACCGG 365 0.0 72.77225 1 TTCGGAG 640 0.0 72.61602 3 GGATCGG 1415 0.0 69.38803 1 >>END_MODULE