FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096019
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT185490.599124230180758Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC55480.17919786667975876No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG55320.17868107398565705No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC53960.17428833608579275No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG47700.1540688219290644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46280.14948228676891193No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA38100.12306126028296337No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36840.11899151781691263No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA32560.10516731324969258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA44150.088.4006654
GAGCGGC36000.087.332599
AGAGCGG37350.087.221678
ATTCGGA6550.084.710642
AAGCGGC12750.084.4269269
GAGAGCG41950.083.083287
TCGGGAG47950.081.790583
CGGAAAG17750.080.952575
GTTCGGA5300.080.530432
TCGGAGA9650.078.13694
GATCCGG9950.076.272421
TCGGGAA14950.076.13013
GATCGGG64650.075.861711
AAGGGGC28600.075.773179
ATCGGGA63250.075.6526262
AAAGCGG15200.074.561158
ATCCGGA9700.074.313072
GAACCGG3650.072.772251
TTCGGAG6400.072.616023
GGATCGG14150.069.388031
Produced by FastQC (version 0.11.2)