FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843261
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70890.3845901367196507No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69980.3796532341323339No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA45510.24689938104261958No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT45330.24592285086051296Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC39610.21489089174023648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC11600.069.908619
CGGGAGA17600.059.8213774
GGAGAGC17050.056.7442556
ATCGGGA22300.055.9343762
AGAGCGG15000.054.6949658
TCGGGAG19900.053.6223143
ATTGGGA9800.052.7507132
AAGCGGC3950.050.4248629
AGAGCGT40200.048.6030658
GATCGGG27000.047.2618641
GATTGGG11900.047.038921
TCGGAGA5750.044.539164
GAGCGTC44600.043.595489
AAGAGCG43350.042.117657
CGGAAGA46100.041.459134
CGGAAAG6600.040.9587825
TGTGTTT407900.040.657441
ATCGGAA50400.040.4637222
TTGTGTT226000.039.419351
GATCGGA52300.039.18361
Produced by FastQC (version 0.11.2)