##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_40825-1.342000000356f6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1843261 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.525894596587243 33.0 33.0 33.0 27.0 33.0 2 31.2759392185914 33.0 33.0 33.0 27.0 33.0 3 31.17589315891781 33.0 33.0 33.0 27.0 33.0 4 34.7843696579052 37.0 37.0 37.0 33.0 37.0 5 34.814324178724554 37.0 37.0 37.0 33.0 37.0 6 35.14803600792291 37.0 37.0 37.0 33.0 37.0 7 34.77660732799099 37.0 37.0 37.0 33.0 37.0 8 34.87165952081664 37.0 37.0 37.0 33.0 37.0 9 34.612070130057546 37.0 37.0 37.0 33.0 37.0 10-11 34.571920362878615 37.0 37.0 37.0 33.0 37.0 12-13 34.52423368150251 37.0 37.0 37.0 27.0 37.0 14-15 36.076872727193816 40.0 37.0 40.0 27.0 40.0 16-17 35.95922579602129 40.0 37.0 40.0 27.0 40.0 18-19 35.921444385792356 40.0 37.0 40.0 27.0 40.0 20-21 35.85924619465176 40.0 37.0 40.0 27.0 40.0 22-23 36.20229853504197 38.5 37.0 40.0 27.0 40.0 24-25 35.65106135267875 37.0 37.0 40.0 27.0 40.0 26-27 35.500701474180815 37.0 37.0 40.0 27.0 40.0 28-29 35.24225869261054 37.0 37.0 40.0 24.5 40.0 30-31 35.23512812347248 37.0 37.0 40.0 24.5 40.0 32-33 35.05641794623767 37.0 37.0 40.0 22.0 40.0 34-35 34.96153230605975 37.0 37.0 40.0 22.0 40.0 36-37 34.79517659192051 37.0 37.0 40.0 22.0 40.0 38-39 34.68388714349189 37.0 35.0 40.0 22.0 40.0 40-41 34.593869777530145 37.0 33.0 40.0 22.0 40.0 42-43 34.28033577447795 37.0 33.0 40.0 22.0 40.0 44-45 34.067054529987885 37.0 33.0 40.0 22.0 40.0 46-47 33.878194135285234 37.0 33.0 40.0 18.5 40.0 48-49 33.68254848336725 37.0 33.0 40.0 15.0 40.0 50-51 32.51576526601496 35.0 33.0 38.5 15.0 38.5 52-53 33.000985210450395 37.0 33.0 37.0 18.5 40.0 54-55 33.585012648778445 37.0 33.0 40.0 18.5 40.0 56-57 33.648936585757525 37.0 33.0 40.0 22.0 40.0 58-59 33.273970696499305 37.0 33.0 37.0 15.0 40.0 60-61 33.033510446974134 37.0 33.0 37.0 15.0 40.0 62-63 32.76115862051006 37.0 33.0 37.0 15.0 40.0 64-65 32.48954841446762 37.0 33.0 37.0 15.0 40.0 66-67 32.29090779873279 37.0 33.0 37.0 15.0 38.5 68-69 32.18913789202939 37.0 33.0 37.0 15.0 37.0 70-71 31.893768977914682 37.0 33.0 37.0 15.0 37.0 72-73 31.8657311688361 37.0 33.0 37.0 15.0 37.0 74-75 31.809963157686298 35.0 33.0 37.0 18.5 37.0 76-77 31.573060461866227 33.0 33.0 37.0 18.5 37.0 78-79 31.327639710274347 33.0 33.0 37.0 15.0 37.0 80-81 31.037595869494336 33.0 33.0 37.0 15.0 37.0 82-83 30.746427120196216 33.0 27.0 37.0 15.0 37.0 84-85 30.56310121030066 33.0 27.0 37.0 15.0 37.0 86-87 30.41835312524922 33.0 27.0 37.0 15.0 37.0 88-89 30.256233382033255 33.0 27.0 37.0 15.0 37.0 90-91 30.11288661779314 33.0 27.0 37.0 15.0 37.0 92-93 29.93987042529517 33.0 27.0 37.0 15.0 37.0 94-95 29.750995653898173 33.0 27.0 37.0 15.0 37.0 96-97 29.591193813572794 33.0 27.0 37.0 15.0 37.0 98-99 29.40293262864022 33.0 27.0 37.0 15.0 37.0 100-101 28.00574362502109 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.35858891042485297 2306 2 0.2218553120163982 2306 3 0.23157876848758718 2306 4 0.2789299504407623 2306 5 0.2984602690939866 2306 6 0.16364318262906608 2306 7 0.40572408671286553 2306 8 0.04073223112606428 2306 9 0.3011001247775198 2306 10-11 0.2704591647068497 2306 12-13 0.3634087840369489 2306 14-15 0.7034287002066577 2306 16-17 0.5855336353055449 2306 18-19 0.5819763306095922 2306 20-21 0.5362502546453882 2306 22-23 0.6989625067463194 2306 24-25 0.9841364314418897 2306 26-27 0.6602822533060717 2306 28-29 0.7783237103312928 2306 30-31 0.7221425706191908 2306 32-33 0.6826140836947516 2306 34-35 0.7110334643159391 2306 36-37 0.8001509774650728 2306 38-39 0.762192374703389 2306 40-41 0.6285221792782991 2306 42-43 0.6686717687488581 2306 44-45 0.8079174707776602 2306 46-47 0.8423563629982169 2306 48-49 0.8664509507723537 2306 50-51 0.8162370515526405 2306 52-53 0.7997213717007554 2306 54-55 1.0111866604308588 2306 56-57 1.0522746419342681 2306 58-59 1.159423271744913 2306 60-61 1.138636512289203 2306 62-63 1.1149128399611783 2306 64-65 1.016300778189077 2306 66-67 0.9893392057094346 2306 68-69 1.090119166911414 2306 70-71 0.9800106594605857 2306 72-73 0.8805306325668347 2306 74-75 0.9205106881522482 2306 76-77 0.838345589318326 2306 78-79 0.7762455585800154 2306 80-81 0.8267975147477777 2306 82-83 0.9328440185289146 2306 84-85 1.0352740683497004 2306 86-87 0.8977666050159385 2306 88-89 0.9577336288013107 2306 90-91 0.8683878491842556 2306 92-93 0.7785424357212811 2306 94-95 0.8009152425912447 2306 96-97 0.8500272189883908 2306 98-99 0.7667238776471699 2306 100-101 0.6777949173165929 2307 1 0.2763009958800744 2307 2 0.29393708535422647 2307 3 0.29454454913595285 2307 4 0.33923856339034586 2307 5 0.31998266205315673 2307 6 0.3738377902199588 2307 7 0.580376303828757 2307 8 0.38169919175207667 2307 9 0.3934378113115784 2307 10-11 0.4441326879603906 2307 12-13 0.4248995926321584 2307 14-15 0.7468860346992727 2307 16-17 0.8022913140322387 2307 18-19 0.7514744630509824 2307 20-21 0.7962595480783179 2307 22-23 0.6420635190429209 2307 24-25 0.8814800400441882 2307 26-27 0.7223436431955221 2307 28-29 0.789488583372794 2307 30-31 0.8659068767391105 2307 32-33 0.8675367641071219 2307 34-35 0.7925090352299335 2307 36-37 0.7264833319197166 2307 38-39 0.5873882243760136 2307 40-41 0.6991855396416966 2307 42-43 0.9506670177862375 2307 44-45 0.864572478141433 2307 46-47 0.7020332886684955 2307 48-49 0.8261925312326355 2307 50-51 0.7623079699833539 2307 52-53 0.8638016989005735 2307 54-55 1.0263627920560268 2307 56-57 1.0203464874902792 2307 58-59 0.915767869576591 2307 60-61 0.9350948049591423 2307 62-63 0.9030563935570015 2307 64-65 1.1410945039019467 2307 66-67 1.1166625639435424 2307 68-69 0.9942515284636642 2307 70-71 0.8994289437908343 2307 72-73 0.954252064687072 2307 74-75 0.7674235605549669 2307 76-77 0.696060136756973 2307 78-79 0.8954338359900973 2307 80-81 0.8373391289217054 2307 82-83 0.8782107209793608 2307 84-85 0.7928618569119017 2307 86-87 0.7376034930688853 2307 88-89 0.8196673726130825 2307 90-91 0.6274805528412983 2307 92-93 1.0363559060976328 2307 94-95 1.005704022157225 2307 96-97 1.0798456088955923 2307 98-99 0.6779383230576776 2307 100-101 0.5506467813263889 2201 1 0.14195377484119476 2201 2 0.009412565825019925 2201 3 0.03899230693891198 2201 4 0.13865189607453487 2201 5 0.09075133418077996 2201 6 0.17294277770357525 2201 7 0.4241822167536924 2201 8 0.07751157992622382 2201 9 0.16673207406225998 2201 10-11 0.11341673880488656 2201 12-13 0.18566682446311944 2201 14-15 0.4928425539688135 2201 16-17 0.29288577112154 2201 18-19 0.4463273296470618 2201 20-21 0.3885800282853964 2201 22-23 0.43146171846924375 2201 24-25 0.6980364530157814 2201 26-27 0.5220049706216159 2201 28-29 0.3849599660981582 2201 30-31 0.589446497068792 2201 32-33 0.49442537846059054 2201 34-35 0.4057036740806126 2201 36-37 0.6081619469629018 2201 38-39 0.44980439302460695 2201 40-41 0.4075888592212138 2201 42-43 0.5957779206291036 2201 44-45 0.6435751842762798 2201 46-47 0.4656740137665807 2201 48-49 0.5281774020319361 2201 50-51 0.6375128572547908 2201 52-53 0.5942258358383015 2201 54-55 0.7334901305095158 2201 56-57 0.9162221675740199 2201 58-59 0.9276138024628295 2201 60-61 0.8933703235673747 2201 62-63 0.7749465699223421 2201 64-65 0.9267981395352933 2201 66-67 0.832801899459632 2201 68-69 0.8193812565924574 2201 70-71 0.7083091841378 2201 72-73 0.7160701045041726 2201 74-75 0.6875986681715034 2201 76-77 0.8650505496555461 2201 78-79 0.8322453843292976 2201 80-81 0.8402760155277598 2201 82-83 0.689528815568309 2201 84-85 0.7174531980918104 2201 86-87 1.0417246686755384 2201 88-89 1.1673696937221507 2201 90-91 1.0744745928531465 2201 92-93 1.0149456933800174 2201 94-95 0.9434035843881823 2201 96-97 1.0950818175479178 2201 98-99 1.0906041093176562 2201 100-101 0.8764926006117584 1101 1 0.1903559786979443 1101 2 -0.08257641489123557 1101 3 0.030496439170317302 1101 4 0.0235664966254987 1101 5 0.043373923712458406 1101 6 0.07245241957685522 1101 7 0.13298111482531993 1101 8 0.11916998213008156 1101 9 0.02091940188595487 1101 10-11 0.09441398398395506 1101 12-13 0.06755938644658954 1101 14-15 0.26959737490545876 1101 16-17 0.20343122566699634 1101 18-19 0.20392512579030608 1101 20-21 0.037337604042974704 1101 22-23 0.10433499670615731 1101 24-25 0.4765571141728131 1101 26-27 0.3735945023020477 1101 28-29 0.43338696334333804 1101 30-31 0.5412068276473079 1101 32-33 0.3747807503614169 1101 34-35 0.45882764102275786 1101 36-37 0.42612888911165925 1101 38-39 0.5043911698841086 1101 40-41 0.33155029172809947 1101 42-43 0.41899004184122646 1101 44-45 0.4075008041109882 1101 46-47 0.4740651983395807 1101 48-49 0.5257531596076959 1101 50-51 0.3183806258498265 1101 52-53 0.4890908496124098 1101 54-55 0.6468262186637901 1101 56-57 0.6759935174362752 1101 58-59 0.7746220669256374 1101 60-61 0.6833152271486469 1101 62-63 0.6552110327322609 1101 64-65 0.7022967621248242 1101 66-67 0.6691765551070219 1101 68-69 0.5821773998431397 1101 70-71 0.7383449968099818 1101 72-73 0.814654126542738 1101 74-75 0.685433378915306 1101 76-77 0.8925574367078859 1101 78-79 0.6978349160097324 1101 80-81 0.6536479163542026 1101 82-83 0.6271100827859399 1101 84-85 0.7737479639320313 1101 86-87 0.758716404212727 1101 88-89 0.7446010986808282 1101 90-91 0.6669842347264243 1101 92-93 0.8038988614240914 1101 94-95 0.46068733094465486 1101 96-97 0.5351396687875898 1101 98-99 0.5500311065628303 1101 100-101 0.895889294826631 2304 1 0.2481674265093936 2304 2 0.03728217096735165 2304 3 0.15725064792452415 2304 4 0.1833106043696091 2304 5 0.14846483280336997 2304 6 0.17575881688325268 2304 7 0.15811242795663105 2304 8 0.2081666151267143 2304 9 0.04219886316541732 2304 10-11 0.11474058331054948 2304 12-13 0.12214524503244917 2304 14-15 0.24904855335663711 2304 16-17 0.21426624250201343 2304 18-19 0.28102276888795075 2304 20-21 0.18193019663556953 2304 22-23 0.3063316297027896 2304 24-25 0.4111295047451975 2304 26-27 0.2285170612246148 2304 28-29 0.14584150879788638 2304 30-31 0.2554627199031998 2304 32-33 0.36034977393044443 2304 34-35 0.2686895938847158 2304 36-37 0.2767416837244596 2304 38-39 0.30191642240937 2304 40-41 0.3361395173173207 2304 42-43 0.17810115295233686 2304 44-45 0.2162718478820338 2304 46-47 0.25049954177392664 2304 48-49 0.3147935636481023 2304 50-51 0.22437389184309353 2304 52-53 0.3229831055046617 2304 54-55 0.5851965890341617 2304 56-57 0.5312359416787018 2304 58-59 0.5924133127168787 2304 60-61 0.4431637119971228 2304 62-63 0.5272346017558291 2304 64-65 0.5555324523605094 2304 66-67 0.5573886763191425 2304 68-69 0.5670090496747875 2304 70-71 0.6444662089311883 2304 72-73 0.7558696147582289 2304 74-75 0.7892515607334829 2304 76-77 0.796378985529433 2304 78-79 0.8834544446292654 2304 80-81 0.7726681183744049 2304 82-83 0.5835141231899819 2304 84-85 0.7486132837973472 2304 86-87 0.48458509108141357 2304 88-89 0.6315034555831822 2304 90-91 0.7219034266456177 2304 92-93 0.696710309235538 2304 94-95 0.9195055127628393 2304 96-97 0.7735100391579586 2304 98-99 0.8546001301318533 2304 100-101 0.6924246483619854 2202 1 0.3979294929895332 2202 2 0.3436300420283622 2202 3 0.40122109473272616 2202 4 0.4698498608039898 2202 5 0.42008541867441807 2202 6 0.47004197335024855 2202 7 0.5805352994849926 2202 8 0.5181270045442332 2202 9 0.355626372891507 2202 10-11 0.4724775250080171 2202 12-13 0.47774815880932664 2202 14-15 0.7040335254298213 2202 16-17 0.6436372713178216 2202 18-19 0.6292267914288061 2202 20-21 0.5844023788733281 2202 22-23 0.5641842667041033 2202 24-25 0.7748857179153532 2202 26-27 0.6783956538545937 2202 28-29 0.6750939343488938 2202 30-31 0.6610682300737878 2202 32-33 0.6082386981302861 2202 34-35 0.5583733946727776 2202 36-37 0.6188757531873321 2202 38-39 0.6244682827773929 2202 40-41 0.5644264998300912 2202 42-43 0.6709793488533577 2202 44-45 0.5917893091490356 2202 46-47 0.6767341269844493 2202 48-49 0.8150303547547253 2202 50-51 0.8405315614862658 2202 52-53 0.8751517173894996 2202 54-55 0.864199033798478 2202 56-57 0.8748323000807332 2202 58-59 0.9720384765493222 2202 60-61 0.9240359756577945 2202 62-63 0.9095990648112249 2202 64-65 0.8656463405743438 2202 66-67 0.9949057346258385 2202 68-69 1.1104737189876985 2202 70-71 1.1085325353664253 2202 72-73 0.969874977869079 2202 74-75 0.9180646764682479 2202 76-77 1.069663132780267 2202 78-79 1.0554929460323592 2202 80-81 1.0036111077998164 2202 82-83 1.0212922748484523 2202 84-85 1.0317296624720242 2202 86-87 1.0822526986811631 2202 88-89 0.9145764225549797 2202 90-91 0.9653944753186536 2202 92-93 0.9523123921664336 2202 94-95 0.8770663151108096 2202 96-97 0.8753379003319033 2202 98-99 0.9906239504863343 2202 100-101 0.9506310180427526 1102 1 0.2315217666299887 1102 2 -0.05109545414137884 1102 3 0.08932830794594793 1102 4 -0.030115987264366595 1102 5 -0.03408829069879005 1102 6 0.06421691869812207 1102 7 0.029515385475583855 1102 8 0.1313504156391616 1102 9 0.1172990152427218 1102 10-11 0.042537592654134926 1102 12-13 -0.015350982621953335 1102 14-15 -0.05207397834585947 1102 16-17 0.012662625784159331 1102 18-19 0.13165681881901037 1102 20-21 0.1493844698894904 1102 22-23 0.14861326266984065 1102 24-25 0.14076801048212673 1102 26-27 0.22917270968342507 1102 28-29 0.22587026738552396 1102 30-31 0.26713184229288345 1102 32-33 0.2013970362430868 1102 34-35 0.4573039151879641 1102 36-37 0.30608905314212365 1102 38-39 0.38442339015359295 1102 40-41 0.43995861041585016 1102 42-43 0.13951493932218995 1102 44-45 0.1868534696060138 1102 46-47 0.24865863712106773 1102 48-49 0.387613147891237 1102 50-51 0.4578533850765396 1102 52-53 0.4472314471519496 1102 54-55 0.6185169040767917 1102 56-57 0.4971516896682644 1102 58-59 0.5622635431997978 1102 60-61 0.7651107748932233 1102 62-63 0.7103492869794721 1102 64-65 0.7257537041283371 1102 66-67 0.692415570924247 1102 68-69 0.7414304754143259 1102 70-71 0.5724738778878908 1102 72-73 0.8005188014109272 1102 74-75 0.7605675324009482 1102 76-77 0.6181491180201242 1102 78-79 0.5936749863085033 1102 80-81 0.5237820698398501 1102 82-83 0.4526589990132379 1102 84-85 0.5969963529185556 1102 86-87 0.4783256602174113 1102 88-89 0.37114474250039464 1102 90-91 0.46003695880375517 1102 92-93 0.36828960541940603 1102 94-95 0.5488765517999568 1102 96-97 0.5584999499826679 1102 98-99 0.5992794955493572 1102 100-101 0.7800726574511181 2305 1 0.22451432128026028 2305 2 0.2302989088231584 2305 3 0.21416421571489863 2305 4 0.27937707746160356 2305 5 0.11055409389373949 2305 6 0.3040536034466257 2305 7 0.18497371563923082 2305 8 0.18009044087961712 2305 9 0.22609143296253364 2305 10-11 0.2472674800993815 2305 12-13 0.25823197246212715 2305 14-15 0.05566951696982869 2305 16-17 0.1741792833806457 2305 18-19 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-0.9990714227754189 1213 78-79 -0.8783093814067726 1213 80-81 -0.7512172380251307 1213 82-83 -0.7728949581718432 1213 84-85 -0.9020018785380373 1213 86-87 -1.0458878806013878 1213 88-89 -0.8263440809032936 1213 90-91 -0.8036641584682833 1213 92-93 -0.6612460661121382 1213 94-95 -0.6913611882739943 1213 96-97 -0.728933425100248 1213 98-99 -0.7519556609229916 1213 100-101 -1.0239966535035485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 824.0 3 99.0 4 126.0 5 146.0 6 254.0 7 2155.0 8 12144.0 9 21071.0 10 21058.0 11 15976.0 12 11524.0 13 9624.0 14 9469.0 15 10365.0 16 10105.0 17 9414.0 18 9177.0 19 9136.0 20 9872.0 21 10415.0 22 10932.0 23 12034.0 24 13426.0 25 15321.0 26 17799.0 27 21013.0 28 24765.0 29 29564.0 30 37466.0 31 49451.0 32 66514.0 33 95309.0 34 145157.0 35 243923.0 36 412291.0 37 416706.0 38 58606.0 39 30.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.58647861793309 22.566771754002175 24.48377340692707 12.36297622113766 2 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20.598589063340086 20-21 25.816549792363247 26.636995998233985 27.158436590554007 20.388017618848757 22-23 28.22972003417357 25.59319005273223 25.438100053823913 20.738989859270287 24-25 26.15250706525306 27.218021031944993 25.588430660025494 21.041041242776448 26-27 24.041886064420513 28.34433994600612 26.038609652086574 21.575164337486793 28-29 24.841334735038743 27.58911843130976 25.387477063683118 22.18206976996838 30-31 26.249820580406073 25.925451702420098 26.18528389616716 21.63944382100666 32-33 24.69448983010654 25.34989263359883 28.13557210953766 21.820045426756966 34-35 23.67465236092125 26.48772336181391 26.56978157881424 23.267842698450604 36-37 24.818495779180335 27.83290460604867 26.109790811183935 21.23880880358706 38-39 25.653126850663227 26.81271485375459 24.786679822102382 22.747478473479802 40-41 25.24534111649997 25.97653625510362 24.20641893637914 24.571703692017266 42-43 26.81582582071781 25.646973975165842 25.32234733798265 22.214852866133697 44-45 24.870329043791624 26.678704367628992 25.680371293619437 22.770595294959943 46-47 25.699629581460243 26.92872585708603 25.14696751450629 22.224677046947434 48-49 24.11129945501196 26.698373742623073 25.867396219638618 23.322930582726357 50-51 23.417206787331178 25.740090698369826 25.899072691203536 24.943629823095463 52-53 26.55318961363074 25.47693802968739 24.2969903647811 23.67288199190077 54-55 27.043475091024238 25.361703007445165 25.03656203685366 22.558259864676938 56-57 27.370903208218316 25.134662024030487 24.628444907329563 22.865989860421635 58-59 26.243101588797558 25.069786727407873 24.99608214863675 23.691029535157817 60-61 24.345309838937773 25.250164500482963 24.85234398567465 25.552181674904613 62-63 26.209470672381347 25.09369948326901 25.17075896105436 23.526070883295283 64-65 25.141665812042767 26.304697856222433 25.89545768986048 22.658178641874322 66-67 24.13018670576602 26.180209760058542 25.187011796453042 24.502591737722398 68-69 23.411897355825793 24.524705571929672 28.55104258615226 23.512354486092278 70-71 23.398385808077705 26.84558136853321 26.74577537832453 23.010257445064553 72-73 23.514874975696706 28.807377868298577 25.16135347934798 22.516393676656737 74-75 23.973414301506732 28.746167223934744 25.102327472387014 22.17809100217151 76-77 23.986433476636442 28.27823882117442 24.826944429412226 22.908383272776913 78-79 23.92561378930016 28.409349861668648 24.98146789499761 22.68356845403358 80-81 24.15758678409664 28.965280922252273 24.746395582370273 22.13073671128081 82-83 23.770853965110078 28.530836117489578 25.030553167408502 22.667756749991838 84-85 23.368185001520928 28.5496271684117 24.963990176814082 23.11819765325329 86-87 23.165306586603492 28.78944350072529 24.93699269049917 23.108257222172043 88-89 23.271997227033378 28.789417236251207 25.118800615755305 22.819784920960114 90-91 23.707188844149453 28.25371040671057 24.722217613180902 23.31688313595907 92-93 23.368819381779076 27.937076991779573 24.451812894315868 24.24229073212548 94-95 23.49803436148768 28.472539661519143 23.98024599237892 24.04917998461426 96-97 23.418776179697552 29.06986546177968 23.655569538606454 23.855788819916313 98-99 23.61665508008882 27.7971250055757 24.341985732734095 24.244234181601385 100-101 24.407304447253107 27.99479644759363 23.84514563119999 23.752753473953273 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3093.0 1 1931.5 2 694.0 3 1099.5 4 1338.5 5 1064.0 6 1392.5 7 2008.0 8 2294.0 9 2233.5 10 1974.5 11 1712.0 12 1517.5 13 1377.0 14 1281.0 15 1219.0 16 1162.0 17 1090.0 18 1073.5 19 1070.5 20 1063.0 21 1090.0 22 1133.5 23 1215.5 24 1424.0 25 1633.0 26 1788.0 27 2110.0 28 2555.0 29 3026.0 30 3824.5 31 4932.0 32 6207.0 33 7936.5 34 10612.5 35 15277.5 36 21050.5 37 28217.5 38 35978.5 39 40696.5 40 46151.0 41 50609.5 42 56861.5 43 68875.5 44 76248.5 45 82173.0 46 94138.0 47 101206.0 48 98737.5 49 95913.5 50 93287.0 51 90340.0 52 87658.5 53 84646.5 54 79252.0 55 70019.0 56 60708.0 57 54215.5 58 49499.5 59 41889.5 60 31957.5 61 23473.5 62 16858.5 63 12574.5 64 9889.5 65 8104.5 66 7415.5 67 6791.5 68 5785.5 69 4367.0 70 2937.0 71 2123.5 72 1866.0 73 1524.0 74 1008.0 75 690.5 76 535.0 77 409.5 78 340.5 79 281.0 80 208.5 81 160.5 82 147.5 83 123.0 84 90.0 85 60.5 86 44.0 87 41.5 88 34.5 89 36.0 90 28.0 91 17.5 92 15.0 93 12.0 94 9.5 95 7.5 96 6.5 97 3.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15662459087454245 2 0.14821558097306892 3 0.11669535676173912 4 0.1115414474672876 5 0.12331406132935054 6 0.14333293006253592 7 0.12016746407589593 8 0.1112701890833691 9 0.10519400128359466 10-11 0.09260761226977623 12-13 0.12038447078303073 14-15 0.11661397924656357 16-17 0.10676729991032198 18-19 0.10475998786932507 20-21 0.09895505845346915 22-23 0.1124094742958268 24-25 0.3981801817539676 26-27 0.2861775950340185 28-29 0.3757742392422994 30-31 0.21684395210444965 32-33 0.2548201258530398 34-35 0.26756926989720936 36-37 0.26442267264375474 38-39 0.28414315715462973 40-41 0.27700906165757316 42-43 0.3294975589458031 44-45 0.2792333804057049 46-47 0.3043519067565581 48-49 0.3041349000494233 50-51 0.3055183178074076 52-53 0.30812239829302523 54-55 0.28693711850899034 56-57 0.29903524243175544 58-59 0.29941500416924133 60-61 0.27779571097093686 62-63 0.2750831271317518 64-65 0.2832208786493069 66-67 0.28256985852790245 68-69 0.28012853307263597 70-71 0.25465737082268874 72-73 0.24513620154714932 74-75 0.25381646983254136 76-77 0.2505071175487356 78-79 0.24738764613367287 80-81 0.2559865369038894 82-83 0.2973534404514607 84-85 0.3020733363316427 86-87 0.29181976941952337 88-89 0.29936075249245764 90-91 0.275951153960291 92-93 0.2599740351474913 94-95 0.24660099682030923 96-97 0.24559734079981077 98-99 0.26729801151329086 100-101 0.2659145937553065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1843261.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.599194143697524 #Duplication Level Percentage of deduplicated Percentage of total 1 84.29821649430735 44.34018255351518 2 7.287102190554131 7.665914057318404 3 2.5940453262535623 4.09334081199487 4 1.3706178501884883 2.883735775955265 5 0.8443894082709393 2.2207101209262508 6 0.6157332307430503 1.9432243046747886 7 0.41037411846691896 1.510974355015314 8 0.32054591312292957 1.3488365373057416 9 0.2580338331762021 1.221513451818976 >10 1.7919353277490275 17.93269080615929 >50 0.1306044448203584 4.709390551502813 >100 0.07390314705392739 7.023236977632055 >500 0.003467285856228884 1.2444680380178774 >1k 8.253478996636337E-4 1.098285783791313 >5k 2.06081537210125E-4 0.7634958743718284 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7089 0.3845901367196507 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6998 0.3796532341323339 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4551 0.24689938104261958 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 4533 0.24592285086051296 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3961 0.21489089174023648 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002441325455266509 0.0 0.0 2 0.00282108719275241 0.0 0.0 3 0.0032008489302383114 0.0 0.0 4 0.003309352283805712 0.0 0.0 5 0.004340134142696015 0.0 0.0 6 0.004828399233749317 0.0 0.0 7 0.00564217438550482 0.0 0.0 8 0.006401697860476623 0.0 0.0 9 0.007649486426501727 0.0 0.0 10-11 0.016329754711893758 0.0 0.0 12-13 0.029621415523900303 0.0 0.0 14-15 0.04600542191257777 0.0 0.0 16-17 0.06759758927249043 0.0 0.0 18-19 0.08856586234939057 0.0 0.0 20-21 0.09613397126071674 0.0 0.0 22-23 0.10044697956502091 0.0 0.0 24-25 0.10657741904157902 0.0 0.0 26-27 0.1151220581350118 0.0 0.0 28-29 0.1252399958551719 0.0 0.0 30-31 0.17951879847726393 0.0 0.0 32-33 0.1892569744599381 0.0 0.0 34-35 0.19685220920965615 0.0 0.0 36-37 0.2050713382423867 0.0 0.0 38-39 0.21261232131532104 0.0 0.0 40-41 0.21917677420614878 0.0 0.0 42-43 0.22734165156209565 0.0 0.0 44-45 0.23596766817070397 0.0 0.0 46-47 0.2463568642747826 0.0 0.0 48-49 0.25593228522710565 0.0 0.0 50-51 0.2671352564829398 0.0 0.0 52-53 0.278853618668219 0.0 0.0 54-55 0.29230803451057663 0.0 0.0 56-57 0.30747137817162085 0.0 0.0 58-59 0.3243165238129597 0.0 0.0 60-61 0.34411838583901033 0.0 0.0 62-63 0.3651137847543023 0.0 0.0 64-65 0.3872755947204438 0.0 0.0 66-67 0.4127467569703911 0.0 0.0 68-69 0.4394928336247552 0.0 0.0 70-71 0.46775795722906305 0.0 0.0 72-73 0.5010956126126468 0.0 0.0 74-75 0.5419199993923812 0.0 0.0 76-77 0.5854569700113006 0.0 0.0 78-79 0.6356126451978314 0.0 0.0 80-81 0.690705222971679 0.0 0.0 82-83 0.746801456766025 0.0 0.0 84-85 0.8150771919983117 0.0 0.0 86-87 0.8899173801214262 0.0 0.0 88-89 0.9700199808925594 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1160 0.0 69.90861 9 CGGGAGA 1760 0.0 59.821377 4 GGAGAGC 1705 0.0 56.744255 6 ATCGGGA 2230 0.0 55.934376 2 AGAGCGG 1500 0.0 54.694965 8 TCGGGAG 1990 0.0 53.622314 3 ATTGGGA 980 0.0 52.750713 2 AAGCGGC 395 0.0 50.424862 9 AGAGCGT 4020 0.0 48.603065 8 GATCGGG 2700 0.0 47.261864 1 GATTGGG 1190 0.0 47.03892 1 TCGGAGA 575 0.0 44.53916 4 GAGCGTC 4460 0.0 43.59548 9 AAGAGCG 4335 0.0 42.11765 7 CGGAAGA 4610 0.0 41.45913 4 CGGAAAG 660 0.0 40.958782 5 TGTGTTT 40790 0.0 40.65744 1 ATCGGAA 5040 0.0 40.463722 2 TTGTGTT 22600 0.0 39.41935 1 GATCGGA 5230 0.0 39.1836 1 >>END_MODULE