FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_40255-1.342000000357f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_40255-1.342000000357f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7679010
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT183150.23850730758261807Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA140300.18270584359181716No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC106570.1387809105600852No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG103640.13496531453924399No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99380.12941772442020522No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC98930.12883171137946167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95120.12387013430116642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA50450.077.634782
GAGCGGC32350.076.056129
CGGGAGA44900.070.32624
AGAGCGG36750.068.366398
AAGCGGC12350.066.791859
ATTCGGA8850.063.2231522
CGGAAAG20000.061.87285
TCGGGAG57900.056.9927943
TCGGGAA16300.056.1382643
GATCGGG77550.053.9984741
GTTCGGA7150.053.7175752
AAGCGTC7000.052.824789
GAACCGG4850.051.8246881
GAGAGCG55350.051.2162447
TCGGAGA11150.050.1760444
GGTTCGG9600.049.8945051
TCGGAAA19500.046.9258274
GATCCGG11600.046.1979871
TTGTGTT623850.045.2845421
ACCGGAA6250.044.7570273
Produced by FastQC (version 0.11.2)