##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_35011-1.342000000358a4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6028003 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51085060840215 33.0 33.0 33.0 27.0 33.0 2 31.284684496673275 33.0 33.0 33.0 27.0 33.0 3 31.163573906648686 33.0 33.0 33.0 27.0 33.0 4 34.728348177663484 37.0 37.0 37.0 33.0 37.0 5 34.75412902083824 37.0 37.0 37.0 33.0 37.0 6 35.05647691283498 37.0 37.0 37.0 33.0 37.0 7 34.70276988913244 37.0 37.0 37.0 33.0 37.0 8 34.881248566067406 37.0 37.0 37.0 33.0 37.0 9 34.5526385769881 37.0 37.0 37.0 33.0 37.0 10-11 34.542769554029746 37.0 37.0 37.0 27.0 37.0 12-13 34.51088287447767 37.0 37.0 37.0 30.0 37.0 14-15 36.14553683865121 40.0 37.0 40.0 27.0 40.0 16-17 36.10626603868644 40.0 37.0 40.0 27.0 40.0 18-19 36.1602297643183 40.0 37.0 40.0 27.0 40.0 20-21 36.13971011626902 40.0 37.0 40.0 27.0 40.0 22-23 36.504041056383016 40.0 37.0 40.0 30.0 40.0 24-25 36.03612299131238 40.0 37.0 40.0 27.0 40.0 26-27 35.952654967159106 40.0 37.0 40.0 27.0 40.0 28-29 35.725351745843525 40.0 37.0 40.0 27.0 40.0 30-31 35.75085256261485 40.0 37.0 40.0 27.0 40.0 32-33 35.60306531035236 40.0 37.0 40.0 27.0 40.0 34-35 35.696865031420856 40.0 37.0 40.0 27.0 40.0 36-37 35.51835027288473 40.0 37.0 40.0 27.0 40.0 38-39 35.46865330027208 40.0 37.0 40.0 27.0 40.0 40-41 35.32719849343141 37.0 37.0 40.0 27.0 40.0 42-43 35.21236046166533 37.0 37.0 40.0 22.0 40.0 44-45 35.10899289532537 37.0 37.0 40.0 22.0 40.0 46-47 34.99355325470143 37.0 37.0 40.0 22.0 40.0 48-49 34.88228456422467 37.0 37.0 40.0 22.0 40.0 50-51 33.866249651833286 37.0 35.0 38.5 22.0 38.5 52-53 34.29916599245223 37.0 35.0 38.5 22.0 40.0 54-55 34.9703186113212 37.0 37.0 40.0 22.0 40.0 56-57 35.16375655420211 37.0 37.0 40.0 24.5 40.0 58-59 34.819023729749304 37.0 37.0 40.0 22.0 40.0 60-61 34.65191067423158 37.0 35.0 40.0 22.0 40.0 62-63 34.37289148661671 37.0 33.0 40.0 22.0 40.0 64-65 34.14255409295583 37.0 33.0 40.0 22.0 40.0 66-67 33.94153644250011 37.0 33.0 38.5 22.0 40.0 68-69 33.86968909935844 37.0 33.0 37.0 22.0 40.0 70-71 33.535469126342505 37.0 33.0 37.0 22.0 40.0 72-73 33.5456147749097 37.0 33.0 37.0 22.0 40.0 74-75 33.4496971882728 37.0 33.0 37.0 27.0 38.5 76-77 33.16762516541548 37.0 33.0 37.0 22.0 37.0 78-79 32.81311721311353 37.0 33.0 37.0 22.0 37.0 80-81 32.4234791356275 37.0 33.0 37.0 22.0 37.0 82-83 32.13248956578157 37.0 33.0 37.0 22.0 37.0 84-85 31.915199527936533 37.0 33.0 37.0 15.0 37.0 86-87 31.75817397569311 35.0 33.0 37.0 15.0 37.0 88-89 31.595396186763676 33.0 33.0 37.0 15.0 37.0 90-91 31.455661933147677 33.0 33.0 37.0 15.0 37.0 92-93 31.30824694347365 33.0 33.0 37.0 15.0 37.0 94-95 31.159241377285312 33.0 33.0 37.0 15.0 37.0 96-97 31.02657264769112 33.0 33.0 37.0 15.0 37.0 98-99 30.87500678085263 33.0 33.0 37.0 15.0 37.0 100-101 29.630052108467765 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17031067147285484 2306 2 0.09747421313233673 2306 3 0.19996779993595126 2306 4 0.28665442649720774 2306 5 0.23045066087652089 2306 6 0.09738393957275093 2306 7 0.41381571842364195 2306 8 0.15375550625078915 2306 9 0.2874929861260611 2306 10-11 0.25711089664005016 2306 12-13 0.21370374937635006 2306 14-15 0.4989898249153626 2306 16-17 0.4539431275358865 2306 18-19 0.437106481097878 2306 20-21 0.41479481174937405 2306 22-23 0.3727189524608363 2306 24-25 0.5719744078234967 2306 26-27 0.4227106005291077 2306 28-29 0.46659886038357 2306 30-31 0.5187270122124232 2306 32-33 0.5236089609494741 2306 34-35 0.43995409770566596 2306 36-37 0.4421213652815581 2306 38-39 0.35661506385500985 2306 40-41 0.3371648295564924 2306 42-43 0.4210901633662445 2306 44-45 0.4730619154464435 2306 46-47 0.3102415046854645 2306 48-49 0.4199493577732767 2306 50-51 0.4050055531699641 2306 52-53 0.4610034372225229 2306 54-55 0.5130464280631202 2306 56-57 0.5269177791445827 2306 58-59 0.6891345149137962 2306 60-61 0.611138435829929 2306 62-63 0.5849987660742784 2306 64-65 0.5871732031574055 2306 66-67 0.5401621948504456 2306 68-69 0.5213996474526539 2306 70-71 0.5583654810615002 2306 72-73 0.3287578276212386 2306 74-75 0.415538045127974 2306 76-77 0.5268299856043157 2306 78-79 0.5124864167821883 2306 80-81 0.5518799975972328 2306 82-83 0.3942780055662496 2306 84-85 0.40789722534031014 2306 86-87 0.508564177898112 2306 88-89 0.4229586154035623 2306 90-91 0.3854698103799912 2306 92-93 0.18929877494480252 2306 94-95 0.2946016060414358 2306 96-97 0.42461141592922047 2306 98-99 0.45116656015223455 2306 100-101 0.4710166123666788 2307 1 0.17366318763291133 2307 2 0.0967880304742259 2307 3 0.1007432389737346 2307 4 0.24313753676346295 2307 5 0.19152363226073987 2307 6 0.15950804195330903 2307 7 0.36407919372688724 2307 8 0.07278506612431812 2307 9 0.2167078014661925 2307 10-11 0.2731332201537029 2307 12-13 0.24607616843477587 2307 14-15 0.4849528048484686 2307 16-17 0.4800528901671086 2307 18-19 0.4187536297416088 2307 20-21 0.4483831619911811 2307 22-23 0.38795270610990684 2307 24-25 0.6248488954026783 2307 26-27 0.3878167302982334 2307 28-29 0.38308037500505066 2307 30-31 0.4078112506216982 2307 32-33 0.387012072345982 2307 34-35 0.3452986489388934 2307 36-37 0.4580069232004078 2307 38-39 0.4531578612086733 2307 40-41 0.37814677716283285 2307 42-43 0.4187352576173211 2307 44-45 0.32359260584537 2307 46-47 0.2865153195452379 2307 48-49 0.24054547514846547 2307 50-51 0.2969547496132279 2307 52-53 0.23399183056890394 2307 54-55 0.30496199407654956 2307 56-57 0.258245244789741 2307 58-59 0.23745515419114582 2307 60-61 0.22714624340846967 2307 62-63 0.304800856258133 2307 64-65 0.4584751717287432 2307 66-67 0.3971307578781378 2307 68-69 0.34328917897023814 2307 70-71 0.3413948846948074 2307 72-73 0.13942695682580108 2307 74-75 0.2905432392841405 2307 76-77 0.36964775576830533 2307 78-79 0.35617946459114336 2307 80-81 0.41075192106390546 2307 82-83 0.4743023890129905 2307 84-85 0.4581257128075755 2307 86-87 0.324976686423458 2307 88-89 0.34780928687637314 2307 90-91 0.32399895278187074 2307 92-93 0.45799167039183786 2307 94-95 0.41692345756557714 2307 96-97 0.4741107546785095 2307 98-99 0.3201667834316986 2307 100-101 0.4337148144406804 2201 1 0.3219813397665945 2201 2 0.30201376529186774 2201 3 0.3579618141430956 2201 4 0.5123698703818462 2201 5 0.47187994729031857 2201 6 0.41612534814195357 2201 7 0.5277332417450324 2201 8 0.3782537085574873 2201 9 0.48321345604726673 2201 10-11 0.4704210675728646 2201 12-13 0.4736941201033602 2201 14-15 0.6865067567393695 2201 16-17 0.6303756932931961 2201 18-19 0.5793907222271386 2201 20-21 0.6475032376803398 2201 22-23 0.5198192041854668 2201 24-25 0.7133834791060139 2201 26-27 0.6783750388629031 2201 28-29 0.5905636474746387 2201 30-31 0.7548480429780042 2201 32-33 0.622662522855606 2201 34-35 0.4803567989757127 2201 36-37 0.6388611798942705 2201 38-39 0.5572390375737584 2201 40-41 0.5778148160533121 2201 42-43 0.546212365816686 2201 44-45 0.6036725392510434 2201 46-47 0.6650375115496203 2201 48-49 0.6226200805025357 2201 50-51 0.5517394485808396 2201 52-53 0.6011158069274174 2201 54-55 0.6972478141076479 2201 56-57 0.6045296930180797 2201 58-59 0.7405086153404525 2201 60-61 0.6559779752526111 2201 62-63 0.5790350909475634 2201 64-65 0.5943462980596479 2201 66-67 0.5013065456857717 2201 68-69 0.5597254223528125 2201 70-71 0.5458222959918402 2201 72-73 0.4508086249430079 2201 74-75 0.4177281762330054 2201 76-77 0.4639353215005215 2201 78-79 0.5030713713338173 2201 80-81 0.6038075169707255 2201 82-83 0.61630671158116 2201 84-85 0.5850207522121629 2201 86-87 0.5404935945871934 2201 88-89 0.5406440806448991 2201 90-91 0.6906508824637179 2201 92-93 0.6874093868581497 2201 94-95 0.5970991392669163 2201 96-97 0.6452497172874097 2201 98-99 0.6081107087833963 2201 100-101 0.46612169669680625 1101 1 0.26319807022656505 1101 2 0.12462701882423488 1101 3 0.16833073023648382 1101 4 0.18450169178773024 1101 5 0.23351953532645808 1101 6 0.21100682576683027 1101 7 0.3241957421033774 1101 8 0.26278897304460713 1101 9 0.23741336412572878 1101 10-11 0.19426381793190473 1101 12-13 0.2506528395641823 1101 14-15 0.4039478053477339 1101 16-17 0.4174774783651074 1101 18-19 0.41584602792173797 1101 20-21 0.3316500550588728 1101 22-23 0.3689083116680152 1101 24-25 0.5014303498434316 1101 26-27 0.44021795937852204 1101 28-29 0.48003426171212027 1101 30-31 0.4168860424545855 1101 32-33 0.38614683007330086 1101 34-35 0.37337816009881664 1101 36-37 0.4562601517416738 1101 38-39 0.3914869547740878 1101 40-41 0.2905787090137295 1101 42-43 0.26024773988833516 1101 44-45 0.13475212258938285 1101 46-47 0.27481559593803695 1101 48-49 0.3368486608881085 1101 50-51 0.21718818567691045 1101 52-53 0.30214948498193195 1101 54-55 0.5735636607706809 1101 56-57 0.5813920751915944 1101 58-59 0.6166440174324563 1101 60-61 0.5655783662428178 1101 62-63 0.6150850725909933 1101 64-65 0.5854688644276536 1101 66-67 0.6463087050096519 1101 68-69 0.4855585422197919 1101 70-71 0.5134725955691337 1101 72-73 0.5150426845551408 1101 74-75 0.4546177742103623 1101 76-77 0.5436953591199156 1101 78-79 0.6492209778005105 1101 80-81 0.5698388553723817 1101 82-83 0.5746447535044155 1101 84-85 0.650164247838493 1101 86-87 0.5558682349695978 1101 88-89 0.5910055015262579 1101 90-91 0.4677563388486057 1101 92-93 0.4295587186699308 1101 94-95 0.4820182270427402 1101 96-97 0.451900203667023 1101 98-99 0.44156786030690043 1101 100-101 0.5302729436947864 2304 1 0.2309070862329463 2304 2 0.10005374604192951 2304 3 0.1277045715403915 2304 4 0.14832667336956007 2304 5 0.1486818795694802 2304 6 0.20095362229527325 2304 7 0.10827357099744717 2304 8 0.16622399117245834 2304 9 0.1357627536603303 2304 10-11 0.1818517578690404 2304 12-13 0.18375580910570477 2304 14-15 0.31303685718510366 2304 16-17 0.28204583097322455 2304 18-19 0.26304805183283975 2304 20-21 0.17674301687112148 2304 22-23 0.2515319607258064 2304 24-25 0.41373870632604337 2304 26-27 0.42318361475270905 2304 28-29 0.3277099117512705 2304 30-31 0.254969324131018 2304 32-33 0.2634416643653097 2304 34-35 0.31877399353295743 2304 36-37 0.19932401436604152 2304 38-39 0.23231625098398467 2304 40-41 0.1975425475703716 2304 42-43 0.27117225593979555 2304 44-45 0.20233980088185888 2304 46-47 0.2742768050553863 2304 48-49 0.2923799769769104 2304 50-51 0.2322752712366949 2304 52-53 0.2342449657513228 2304 54-55 0.4311680090541401 2304 56-57 0.5130491219365609 2304 58-59 0.5472012381352869 2304 60-61 0.3501696727941308 2304 62-63 0.46331297656453785 2304 64-65 0.4484508618409535 2304 66-67 0.411642740124158 2304 68-69 0.5008672865099797 2304 70-71 0.47847352207798366 2304 72-73 0.546734708353334 2304 74-75 0.6925838892548128 2304 76-77 0.6133829598687939 2304 78-79 0.4916875916723029 2304 80-81 0.42853850467892585 2304 82-83 0.45995931042799043 2304 84-85 0.4812995643667115 2304 86-87 0.45802224708446815 2304 88-89 0.48067771900243983 2304 90-91 0.4592391105329945 2304 92-93 0.44612609028976635 2304 94-95 0.47671244059174 2304 96-97 0.39683390272942276 2304 98-99 0.2854174499279907 2304 100-101 0.3193230964970688 2202 1 0.2517069160563885 2202 2 0.14900463164234523 2202 3 0.1643481198821739 2202 4 0.22844965001443285 2202 5 0.21274733332680995 2202 6 0.2907000431691884 2202 7 0.2155834167548747 2202 8 0.2043405300060286 2202 9 0.20685718158213007 2202 10-11 0.1990357973949486 2202 12-13 0.2585997804807221 2202 14-15 0.34588697337876795 2202 16-17 0.2469962429690682 2202 18-19 0.27598217263410874 2202 20-21 0.304351093033155 2202 22-23 0.2549159255988016 2202 24-25 0.3906419344629697 2202 26-27 0.3200345256387962 2202 28-29 0.3831409521160225 2202 30-31 0.3689960868029516 2202 32-33 0.3556691711583255 2202 34-35 0.24907342801698462 2202 36-37 0.31790183602581834 2202 38-39 0.25536425763050374 2202 40-41 0.23337225863144084 2202 42-43 0.255638768484296 2202 44-45 0.2446345755785373 2202 46-47 0.39400723174099284 2202 48-49 0.3368249766357465 2202 50-51 0.3357820722786258 2202 52-53 0.3519104965697366 2202 54-55 0.48677073107635493 2202 56-57 0.5429945771387921 2202 58-59 0.4640815084912546 2202 60-61 0.5400674063153019 2202 62-63 0.459572443638983 2202 64-65 0.4298793013941591 2202 66-67 0.42500567374326437 2202 68-69 0.47492041852123634 2202 70-71 0.49281388723513686 2202 72-73 0.6311435254255784 2202 74-75 0.5131579341188015 2202 76-77 0.6172562626185041 2202 78-79 0.5381614651556603 2202 80-81 0.45551981736630864 2202 82-83 0.45847341621841053 2202 84-85 0.4503418797311731 2202 86-87 0.5378800453048349 2202 88-89 0.481799559799061 2202 90-91 0.3471593844209231 2202 92-93 0.43313345122862756 2202 94-95 0.45430550589699337 2202 96-97 0.35139401238092205 2202 98-99 0.42566388484950224 2202 100-101 0.4389490893608894 1102 1 0.14114448238111166 1102 2 0.04918870569911604 1102 3 0.11973819269754671 1102 4 0.06617175024736355 1102 5 0.0990245389785116 1102 6 0.04270443478374375 1102 7 0.11887852924828479 1102 8 0.2771395502354892 1102 9 0.10048046381344733 1102 10-11 0.1517756509633088 1102 12-13 0.14454661391305024 1102 14-15 0.06554255199734627 1102 16-17 0.13574036134243528 1102 18-19 0.16921504834073886 1102 20-21 0.1851034406781693 1102 22-23 0.1596093802243459 1102 24-25 0.21410977489652794 1102 26-27 0.29217770695640866 1102 28-29 0.1579013822005848 1102 30-31 0.3053512469500461 1102 32-33 0.16779570038124803 1102 34-35 0.3197386107895781 1102 36-37 0.21109289795560215 1102 38-39 0.1655666856994671 1102 40-41 0.24402486056295203 1102 42-43 0.21857070513291177 1102 44-45 0.23109104987290152 1102 46-47 0.26818036680649726 1102 48-49 0.25024019111072704 1102 50-51 0.17901337955148477 1102 52-53 0.17461614493355881 1102 54-55 0.4071974451742406 1102 56-57 0.47578385305632054 1102 58-59 0.4576496619441386 1102 60-61 0.46231860933737323 1102 62-63 0.5019038370088467 1102 64-65 0.42286019319892887 1102 66-67 0.5039983054416481 1102 68-69 0.4456806750769573 1102 70-71 0.43394412730561527 1102 72-73 0.5598887784106097 1102 74-75 0.46854537063060775 1102 76-77 0.4723699240103443 1102 78-79 0.5059779717530972 1102 80-81 0.43306568571131265 1102 82-83 0.29328099942882346 1102 84-85 0.3787205196654959 1102 86-87 0.36947951403961454 1102 88-89 0.5014632475539322 1102 90-91 0.4624325922350643 1102 92-93 0.37877677604124926 1102 94-95 0.4170911443830221 1102 96-97 0.47082754082296674 1102 98-99 0.5136187139569692 1102 100-101 0.4463058780094329 2305 1 0.19231010141728078 2305 2 0.10678191212929278 2305 3 0.06391622279580034 2305 4 0.023556649240788374 2305 5 0.11908800454155966 2305 6 0.2151062885736934 2305 7 0.07996856281984321 2305 8 0.18263794118100662 2305 9 0.023507821275742913 2305 10-11 0.03095586926058047 2305 12-13 0.0175775494493422 2305 14-15 -0.07265330398164593 2305 16-17 -0.07187897042198443 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-0.5874018840921913 1213 86-87 -0.7776991177525474 1213 88-89 -0.67726487079943 1213 90-91 -0.6577378775094864 1213 92-93 -0.48543962625254267 1213 94-95 -0.45178048804381277 1213 96-97 -0.5699172226304867 1213 98-99 -0.6942288700609502 1213 100-101 -1.0701888386411156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3092.0 3 308.0 4 430.0 5 448.0 6 1097.0 7 5980.0 8 22562.0 9 60542.0 10 86550.0 11 67155.0 12 45255.0 13 32341.0 14 23957.0 15 19532.0 16 17633.0 17 17018.0 18 17738.0 19 18728.0 20 19940.0 21 22350.0 22 24639.0 23 26852.0 24 30240.0 25 34594.0 26 40505.0 27 48773.0 28 59209.0 29 72543.0 30 89220.0 31 115163.0 32 155941.0 33 223488.0 34 338757.0 35 563725.0 36 1083914.0 37 1932075.0 38 704694.0 39 1015.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.53607750598245 24.855291145971815 23.327456128689178 15.281175219356555 2 29.99763376458417 25.172889721797848 31.995125289174243 12.834351224443743 3 36.92736646005035 20.58994869614112 26.770514691408575 15.71217015239995 4 28.179559323818808 19.62030709566355 34.193705703283484 18.006427877234156 5 33.28931024795961 18.292239859697595 31.591231863942422 16.827218028400374 6 27.690007553545264 18.766389415333 37.20685980214563 16.336743228976104 7 33.21525749768267 19.7698949809464 29.25195934789181 17.762888173479116 8 27.668828814283696 28.833468457178036 26.379745674832733 17.117957053705542 9 31.104796733073382 25.699874784643374 25.37184675792877 17.82348172435448 10-11 24.702602895682315 29.990315729794 29.094676424726533 16.21240494979715 12-13 25.96520199580453 26.23137239137065 28.986293296087528 18.81713231673729 14-15 23.696782681791465 26.560617890940698 30.36077607790513 19.381823349362715 16-17 24.0502385947079 27.28941297231039 31.227753721761864 17.43259471121985 18-19 23.6591703439902 27.523418205548055 31.42917220337874 17.388239247083007 20-21 23.116980003366415 28.9561939790294 30.508665285389373 17.41816073221481 22-23 24.815054124220776 27.63373258631887 29.849071740961165 17.70214154849919 24-25 22.589574086300253 29.607815012192212 29.778657618589012 18.023953282918523 26-27 21.760137929439296 30.591620746625793 29.5014044131663 18.146836910768616 28-29 22.258745349657847 29.742178993673246 29.334926645862 18.664149010806906 30-31 23.39661751676003 28.218673772212522 29.70381199832337 18.680896712704072 32-33 21.620308703416377 29.435831831887775 30.761841681548052 18.182017783147796 34-35 24.065249501638856 28.087231457663297 29.787623209530604 18.05989583116724 36-37 22.379233421887452 27.89942925014015 30.749976294999993 18.971361032972407 38-39 21.181192058555627 29.019304046701144 31.1030975340073 18.696406360735924 40-41 21.653672900141974 29.920465188515326 29.403614940409412 19.02224697093329 42-43 22.75036959144269 28.360876725335753 30.347173751175443 18.541579932046112 44-45 21.455868322337153 29.190363756199467 30.805089197720886 18.548678723742494 46-47 22.32994949074277 29.489023653836057 29.474611694639208 18.706415160781965 48-49 20.736492258289022 29.406170103460795 30.23237198289283 19.624965655357357 50-51 20.687881370909952 28.154946135025398 30.767331667585857 20.389840826478796 52-53 23.317601397509158 27.861727942219687 28.89052947559872 19.930141184672433 54-55 24.048108229706596 28.066231594107983 28.611744496535124 19.27391567965029 56-57 24.12775113808852 28.268344019948383 28.576624082454742 19.027280759508354 58-59 23.244665582240934 28.28306007746294 28.82950248155991 19.642771858736214 60-61 21.24022921239331 28.60478164969868 28.52199433249606 21.632994805411954 62-63 22.512662936184093 28.433229760576488 28.774127290760116 20.279980012479307 64-65 21.02713386272992 29.043894926641023 29.974922794251608 19.95404841637745 66-67 20.504296014852557 28.399685904790285 29.557186150827096 21.538831929530065 68-69 20.161298373123714 28.00713062361428 32.11518374607806 19.71638725718395 70-71 19.82401837111081 30.622218917746498 30.137475615630482 19.41628709551221 72-73 19.866561577311607 32.30157994937682 28.245048240704072 19.586810232607494 74-75 20.082071340789923 31.90636894529046 28.33037249214561 19.681187221774003 76-77 19.899784384989097 31.866563024208645 28.43908520422967 19.79456738657259 78-79 19.72606899983634 31.987010140147188 28.519687865486375 19.767232994530097 80-81 19.56147123919197 32.04284588435796 28.39219316942245 20.00348970702762 82-83 19.34197956103127 31.620711741249508 28.49498527557563 20.542323422143596 84-85 19.454423336178476 31.212651269172465 28.84887569180804 20.48404970284102 86-87 19.886542040679135 30.999140558246108 29.121830236542003 19.99248716453276 88-89 20.161969109848844 31.082844340782795 28.638322571669818 20.116863977698547 90-91 20.265168451467652 30.85910435668347 28.540932993328987 20.334794198519894 92-93 19.90823361562 31.34684431311488 28.631720741553295 20.113201329711828 94-95 19.57792568777135 31.51027830682964 28.69097770752889 20.22081829787011 96-97 19.832085281071837 31.54847820061602 28.585925423219155 20.033511095092994 98-99 20.39502686790228 31.001335493279115 28.131871477237226 20.471766161581378 100-101 20.570769096406334 30.9093994202561 27.533019201417336 20.986812281920226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2599.0 1 1546.5 2 423.5 3 368.0 4 431.5 5 524.0 6 688.0 7 965.0 8 1338.5 9 1738.0 10 2124.0 11 2428.0 12 2485.0 13 2415.0 14 2280.0 15 2153.0 16 2097.5 17 2110.0 18 2245.5 19 2499.0 20 2930.5 21 3532.0 22 4513.0 23 5986.5 24 8010.0 25 11210.0 26 16260.0 27 22723.5 28 30466.5 29 40005.0 30 51055.5 31 62722.5 32 80070.0 33 108585.0 34 145085.5 35 189959.0 36 251451.0 37 315076.0 38 369755.0 39 400714.0 40 407059.5 41 394333.0 42 367199.5 43 345609.0 44 318702.5 45 292866.0 46 279075.5 47 255628.0 48 222523.5 49 195186.0 50 166085.0 51 138170.0 52 113762.5 53 90182.0 54 72716.5 55 58221.5 56 43198.0 57 30617.5 58 22341.5 59 16733.0 60 12246.0 61 8785.5 62 6224.0 63 4438.5 64 3242.5 65 2383.5 66 1751.5 67 1252.0 68 954.0 69 759.5 70 585.0 71 452.0 72 364.0 73 292.5 74 254.0 75 226.5 76 185.5 77 154.5 78 144.0 79 124.5 80 94.5 81 90.0 82 82.5 83 67.5 84 58.5 85 48.5 86 46.0 87 44.5 88 35.0 89 30.5 90 26.5 91 19.0 92 11.0 93 13.0 94 15.0 95 7.0 96 4.0 97 4.5 98 3.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17257788358764917 2 0.1659753653075488 3 0.1325148643754822 4 0.12713994999670705 5 0.13991366626725302 6 0.1598705242847424 7 0.13508619687150122 8 0.12607823851447986 9 0.11904439994472465 10-11 0.10588083648929837 12-13 0.1356170526126148 14-15 0.13124578736938253 16-17 0.12164891092456323 18-19 0.11955037182297355 20-21 0.11221792689884195 22-23 0.12596211382111122 24-25 0.4213999229927391 26-27 0.3077884997734739 28-29 0.39383689755960644 30-31 0.22471787091015052 32-33 0.26574306615308585 34-35 0.27869926408463963 36-37 0.27559707584750703 38-39 0.2950645512286573 40-41 0.28806389114272174 42-43 0.3432977720814008 44-45 0.2904361527358231 46-47 0.3169291720657737 48-49 0.3161909508007876 50-51 0.3166720388161718 52-53 0.3203797343830121 54-55 0.2981252663610154 56-57 0.3101275828827557 58-59 0.309853860391244 60-61 0.28899288868967055 62-63 0.2862058960488241 64-65 0.29469129328568683 66-67 0.29401942898833994 68-69 0.29178817595147183 70-71 0.2646813546708586 72-73 0.25451214938015126 74-75 0.26404266885733135 76-77 0.25989535837988137 78-79 0.25658580461887626 80-81 0.26604167250746225 82-83 0.3085598995222796 84-85 0.3140343493525136 86-87 0.303384056046422 88-89 0.3103681268904478 90-91 0.28691923345094555 92-93 0.2708110795565297 94-95 0.25568169093479215 96-97 0.25493517504885116 98-99 0.2777204988119614 100-101 0.27750483866713405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6028003.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.81811381596162 #Duplication Level Percentage of deduplicated Percentage of total 1 92.40472449907604 55.27476327218308 2 3.8085303085486846 4.556381989366094 3 1.1190150192866262 2.0081210335637367 4 0.6175417719200526 1.4776073599529727 5 0.3361810546489759 1.0054858294881226 6 0.21438497419086688 0.7694462871948762 7 0.13296578597826889 0.5567633763493818 8 0.13129063444310615 0.6282846491270028 9 0.107971501093928 0.5812786387185128 >10 0.9115893073871104 11.184782555458444 >50 0.12675622145325002 5.270329213941035 >100 0.07522963456892634 9.006134731105849 >500 0.011016148881094215 4.602605303513823 >1k 0.002691208190364182 2.2930566407751716 >5k 2.7982604164708555E-5 0.1622813669467396 >10k+ 8.394772867271519E-5 0.6226777523152011 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 13272 0.22017241862686532 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 12538 0.20799591506507212 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 11390 0.18895146535262175 Illumina Single End PCR Primer 1 (97% over 34bp) TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9695 0.1608327003155108 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.124083050389988E-4 0.0 0.0 2 0.0010285329984075988 0.0 0.0 3 0.0011446576917761985 0.0 0.0 4 0.0012110146594153984 0.0 0.0 5 0.0013271393527839984 0.0 0.0 6 0.0014266748042427982 0.0 0.0 7 0.001559388739521198 0.0 0.0 8 0.0017916381262583977 0.0 0.0 9 0.0020072982710857973 0.0 0.0 10-11 0.003135366720952196 0.0 0.0 12-13 0.004396149106096994 0.0 0.0 14-15 0.005980421708482892 0.0 1.658924190979998E-5 16-17 0.00734903416604139 0.0 1.658924190979998E-5 18-19 0.008983074494156688 0.0 1.658924190979998E-5 20-21 0.010733239515640586 0.0 1.658924190979998E-5 22-23 0.012616118472402883 0.0 3.317848381959996E-5 24-25 0.01489713923500038 0.0 3.317848381959996E-5 26-27 0.017418704005289977 0.0 3.317848381959996E-5 28-29 0.020064688089903075 0.0 3.317848381959996E-5 30-31 0.029263422728887163 0.0 3.317848381959996E-5 32-33 0.03246514641747855 0.0 3.317848381959996E-5 34-35 0.03581617328325816 0.0 4.147310477449995E-5 36-37 0.03965658278537685 0.0 4.9767725729399935E-5 38-39 0.043762420158052345 0.0 9.124083050389989E-5 40-41 0.04824151547369834 0.0 9.953545145879987E-5 42-43 0.05297774403894623 0.0 9.953545145879987E-5 44-45 0.05804575744239013 0.0 9.953545145879987E-5 46-47 0.06338749333734572 0.0 1.1612469336859986E-4 48-49 0.06911907641718161 0.0 1.1612469336859986E-4 50-51 0.0751658550933037 0.0 1.1612469336859986E-4 52-53 0.0817351948895845 0.0 1.1612469336859986E-4 54-55 0.08889345277366317 0.0 1.1612469336859986E-4 56-57 0.09633372777020847 0.0 1.1612469336859986E-4 58-59 0.10467811645083785 0.0 1.2441931432349983E-4 60-61 0.11269072029327125 0.0 1.3271393527839983E-4 62-63 0.12175674099697695 0.0 1.3271393527839983E-4 64-65 0.13122090350651783 0.0 1.3271393527839983E-4 66-67 0.1414232872810448 0.0 1.3271393527839983E-4 68-69 0.1531601759322283 0.0 1.3271393527839983E-4 70-71 0.16658087263725646 0.0 1.3271393527839983E-4 72-73 0.18049924659957867 0.0 1.3271393527839983E-4 74-75 0.19698895305791986 0.0 1.3271393527839983E-4 76-77 0.21472285265949603 0.0 1.3271393527839983E-4 78-79 0.2354096373210166 0.0 1.493031771881998E-4 80-81 0.25943915422736186 0.0 1.493031771881998E-4 82-83 0.2846796857931225 0.0 1.493031771881998E-4 84-85 0.3132878334665726 0.0 1.493031771881998E-4 86-87 0.3474284933169409 0.0 1.493031771881998E-4 88-89 0.38592382916863177 0.0 1.493031771881998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 4320 0.0 80.044586 4 GAGCGGC 3350 0.0 79.56498 9 AGAGCGG 3500 0.0 77.37397 8 GAGAGCG 4085 0.0 75.23387 7 GATCGGG 6075 0.0 70.44196 1 AGAGCGT 8630 0.0 66.33203 8 TCGGGAG 5140 0.0 66.07514 3 CGGAAAG 1875 0.0 65.77491 5 GAGCGTC 8605 0.0 65.53321 9 AAGCGGC 1375 0.0 64.50143 9 ATCGGGA 6500 0.0 64.222374 2 GTTCGGA 740 0.0 62.821945 2 AAGAGCG 8955 0.0 61.277103 7 CGGAAGA 9505 0.0 60.08451 4 TCGGAAG 9700 0.0 59.561237 3 GATCCGG 890 0.0 57.57115 1 TCGGGAA 1760 0.0 57.136204 3 GATCGGA 10935 0.0 56.836025 1 ATCGGAA 11155 0.0 55.453 2 AAGGGGC 3565 0.0 53.613724 9 >>END_MODULE