FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6028003
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC132720.22017241862686532No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG125380.20799591506507212No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT113900.18895146535262175Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA96950.1608327003155108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA43200.080.0445864
GAGCGGC33500.079.564989
AGAGCGG35000.077.373978
GAGAGCG40850.075.233877
GATCGGG60750.070.441961
AGAGCGT86300.066.332038
TCGGGAG51400.066.075143
CGGAAAG18750.065.774915
GAGCGTC86050.065.533219
AAGCGGC13750.064.501439
ATCGGGA65000.064.2223742
GTTCGGA7400.062.8219452
AAGAGCG89550.061.2771037
CGGAAGA95050.060.084514
TCGGAAG97000.059.5612373
GATCCGG8900.057.571151
TCGGGAA17600.057.1362043
GATCGGA109350.056.8360251
ATCGGAA111550.055.4532
AAGGGGC35650.053.6137249
Produced by FastQC (version 0.11.2)