##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6886504 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.101949261918676 33.0 33.0 33.0 33.0 33.0 2 32.0573366398974 33.0 33.0 33.0 33.0 33.0 3 32.002780075347374 33.0 33.0 33.0 33.0 33.0 4 35.77466171514603 37.0 37.0 37.0 33.0 37.0 5 35.76720757005296 37.0 37.0 37.0 33.0 37.0 6 35.86203173627722 37.0 37.0 37.0 33.0 37.0 7 35.73572425137632 37.0 37.0 37.0 33.0 37.0 8 35.73831003365423 37.0 37.0 37.0 33.0 37.0 9 35.65987371821755 37.0 37.0 37.0 33.0 37.0 10-11 35.60604241281207 37.0 37.0 37.0 33.0 37.0 12-13 35.58479164464292 37.0 37.0 37.0 33.0 37.0 14-15 37.36040435030605 40.0 37.0 40.0 33.0 40.0 16-17 37.34177399737225 40.0 37.0 40.0 33.0 40.0 18-19 37.36258063598017 40.0 37.0 40.0 33.0 40.0 20-21 37.33782293599191 40.0 37.0 40.0 33.0 40.0 22-23 37.39587394416674 40.0 37.0 40.0 33.0 40.0 24-25 37.132622880927684 40.0 37.0 40.0 33.0 40.0 26-27 37.069729139778325 40.0 37.0 40.0 33.0 40.0 28-29 36.94025371944894 40.0 37.0 40.0 33.0 40.0 30-31 36.978637418928386 40.0 37.0 40.0 33.0 40.0 32-33 36.88143882585416 40.0 37.0 40.0 33.0 40.0 34-35 36.86681515032882 40.0 37.0 40.0 33.0 40.0 36-37 36.77476655789353 40.0 37.0 40.0 33.0 40.0 38-39 36.72680630113625 40.0 37.0 40.0 33.0 40.0 40-41 36.62207347879272 40.0 37.0 40.0 33.0 40.0 42-43 36.517769030555996 40.0 37.0 40.0 33.0 40.0 44-45 36.43165516203868 40.0 37.0 40.0 33.0 40.0 46-47 36.30404984880572 40.0 37.0 40.0 33.0 40.0 48-49 36.19012092347583 37.0 37.0 40.0 33.0 40.0 50-51 35.09289227160835 37.0 35.0 38.5 30.0 38.5 52-53 35.51476895969276 37.0 37.0 38.5 33.0 40.0 54-55 36.23957773058724 37.0 37.0 40.0 33.0 40.0 56-57 36.32993642347409 37.0 37.0 40.0 33.0 40.0 58-59 36.13348427591126 37.0 37.0 40.0 33.0 40.0 60-61 35.97216229018382 37.0 37.0 40.0 30.0 40.0 62-63 35.75940644193338 37.0 37.0 40.0 27.0 40.0 64-65 35.52941753900092 37.0 37.0 40.0 27.0 40.0 66-67 35.30282782090884 37.0 37.0 40.0 27.0 40.0 68-69 35.10926531081664 37.0 33.0 38.5 27.0 40.0 70-71 34.82011235308946 37.0 33.0 37.0 27.0 40.0 72-73 34.62207115540774 37.0 33.0 37.0 27.0 40.0 74-75 34.402286994968705 37.0 33.0 37.0 27.0 40.0 76-77 34.13274471342788 37.0 33.0 37.0 27.0 37.0 78-79 33.849161417752754 37.0 33.0 37.0 27.0 37.0 80-81 33.54643430106191 37.0 33.0 37.0 27.0 37.0 82-83 33.30385853257328 37.0 33.0 37.0 27.0 37.0 84-85 33.10829340983466 37.0 33.0 37.0 27.0 37.0 86-87 32.95208846172165 37.0 33.0 37.0 27.0 37.0 88-89 32.79529475333202 37.0 33.0 37.0 27.0 37.0 90-91 32.64164995765631 37.0 33.0 37.0 27.0 37.0 92-93 32.49363348950352 33.0 33.0 37.0 24.5 37.0 94-95 32.346694345926466 33.0 33.0 37.0 22.0 37.0 96-97 32.21980347357672 33.0 33.0 37.0 22.0 37.0 98-99 32.06750805633744 33.0 33.0 37.0 22.0 37.0 100-101 30.774534582423826 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3904879079590202 2306 2 0.3412090650772157 2306 3 0.32769506880099186 2306 4 0.4361141682040284 2306 5 0.46461830082117217 2306 6 0.33289964239821757 2306 7 0.4392962476186426 2306 8 0.3177905926558253 2306 9 0.43727748767663854 2306 10-11 0.4384141755448283 2306 12-13 0.461438918859308 2306 14-15 0.7641919873296033 2306 16-17 0.673323052702969 2306 18-19 0.68188594770956 2306 20-21 0.6475496150656213 2306 22-23 0.7183365392085719 2306 24-25 0.8231775842609821 2306 26-27 0.8090821513490454 2306 28-29 0.7507397702926681 2306 30-31 0.6763391756289749 2306 32-33 0.7158045275053837 2306 34-35 0.779357080957908 2306 36-37 0.7274539130043678 2306 38-39 0.7215569933049366 2306 40-41 0.6653544649027552 2306 42-43 0.6913476071368692 2306 44-45 0.6756368631631275 2306 46-47 0.6128070080845589 2306 48-49 0.6640289730891169 2306 50-51 0.6411298442097291 2306 52-53 0.6787642950793753 2306 54-55 0.8656441197088185 2306 56-57 0.7505301107327611 2306 58-59 0.896410398599528 2306 60-61 0.8713364627314633 2306 62-63 0.801412735118106 2306 64-65 0.8239974764590201 2306 66-67 0.6922295596134944 2306 68-69 0.6986210147678733 2306 70-71 0.7029123585152277 2306 72-73 0.5980596255329189 2306 74-75 0.6755618557536991 2306 76-77 0.6428978293841396 2306 78-79 0.5541060214674687 2306 80-81 0.6235318168145341 2306 82-83 0.5728042981234793 2306 84-85 0.5867756512775202 2306 86-87 0.7015164646674705 2306 88-89 0.682181236963288 2306 90-91 0.5902156270017329 2306 92-93 0.5333112419354293 2306 94-95 0.5655429796347704 2306 96-97 0.6053332499822446 2306 98-99 0.707104387404847 2306 100-101 0.6075127971889991 2307 1 0.2520411532821143 2307 2 0.21906241921137592 2307 3 0.2773487349476369 2307 4 0.28667471566794234 2307 5 0.2790952079646871 2307 6 0.34005474406446723 2307 7 0.3248410502510879 2307 8 0.29903224501099146 2307 9 0.34230214715041285 2307 10-11 0.2864146737160098 2307 12-13 0.34112158381296354 2307 14-15 0.5711880937284874 2307 16-17 0.5398350415858886 2307 18-19 0.5181328308622 2307 20-21 0.5411158914981868 2307 22-23 0.4678092643360756 2307 24-25 0.6125312989137726 2307 26-27 0.607823770929798 2307 28-29 0.5377357980329407 2307 30-31 0.5053579619266557 2307 32-33 0.49329169959731445 2307 34-35 0.45743424505288033 2307 36-37 0.5510662833815445 2307 38-39 0.4582757756696765 2307 40-41 0.39468841002526034 2307 42-43 0.37762262385182055 2307 44-45 0.39261296405589974 2307 46-47 0.26229203017451397 2307 48-49 0.3811833930474293 2307 50-51 0.39368425630217985 2307 52-53 0.36874237554712863 2307 54-55 0.4028236579827862 2307 56-57 0.4976304922270103 2307 58-59 0.3203269418158783 2307 60-61 0.27970298562767226 2307 62-63 0.41392485509870625 2307 64-65 0.4462010449378937 2307 66-67 0.4897902775043974 2307 68-69 0.37145261979678423 2307 70-71 0.3833904717574015 2307 72-73 0.39667945536812965 2307 74-75 0.44402345068807847 2307 76-77 0.4784221545588281 2307 78-79 0.46019737033150676 2307 80-81 0.47518889150089194 2307 82-83 0.47380247586571755 2307 84-85 0.5435019848686764 2307 86-87 0.4354845554919322 2307 88-89 0.45360120838399354 2307 90-91 0.45478508910795057 2307 92-93 0.4592360967447533 2307 94-95 0.4156033370070489 2307 96-97 0.5147051872126767 2307 98-99 0.5264543002248914 2307 100-101 0.5408779435595541 2201 1 0.306221897372275 2201 2 0.3362018829901814 2201 3 0.32352776842076025 2201 4 0.4354886059385592 2201 5 0.37442331585634037 2201 6 0.4152064591680187 2201 7 0.46830422495406765 2201 8 0.44081333178949933 2201 9 0.3779101077715765 2201 10-11 0.4249528569235892 2201 12-13 0.40545899888117987 2201 14-15 0.6073241725230787 2201 16-17 0.5704809840630887 2201 18-19 0.5519328176616014 2201 20-21 0.5567775282841438 2201 22-23 0.5341428465223785 2201 24-25 0.6905200132477916 2201 26-27 0.5861228829775627 2201 28-29 0.6052793960288909 2201 30-31 0.6689081758526427 2201 32-33 0.5080521976761219 2201 34-35 0.6548961848123867 2201 36-37 0.6473462562534564 2201 38-39 0.5640966190411589 2201 40-41 0.5058506004926002 2201 42-43 0.5053129641288407 2201 44-45 0.48052192491913104 2201 46-47 0.48427841641976244 2201 48-49 0.5679928389903566 2201 50-51 0.510239861854302 2201 52-53 0.5476715243842705 2201 54-55 0.682577641913646 2201 56-57 0.6953938001882634 2201 58-59 0.7572451608948114 2201 60-61 0.6770135421817471 2201 62-63 0.687324214031598 2201 64-65 0.6933258891929412 2201 66-67 0.671367808575944 2201 68-69 0.7600651858456899 2201 70-71 0.6454255919454823 2201 72-73 0.6434932098665271 2201 74-75 0.6098336318912772 2201 76-77 0.5917188736446803 2201 78-79 0.6776136136044428 2201 80-81 0.7312457262059269 2201 82-83 0.5948933162402312 2201 84-85 0.6354684120320115 2201 86-87 0.6592522930926279 2201 88-89 0.6762219437402948 2201 90-91 0.7698934022943646 2201 92-93 0.6286516330982366 2201 94-95 0.6700240552082235 2201 96-97 0.660196989140772 2201 98-99 0.5927941908293803 2201 100-101 0.6429214803073613 1101 1 0.3065452051293889 1101 2 0.2602589652768117 1101 3 0.3105453682021846 1101 4 0.38246663973156103 1101 5 0.3962529139478619 1101 6 0.3404295238118564 1101 7 0.3767808079098245 1101 8 0.32303140514848394 1101 9 0.2945685526056039 1101 10-11 0.3144902586726843 1101 12-13 0.37763264904599225 1101 14-15 0.534203493728576 1101 16-17 0.4678532863533178 1101 18-19 0.4623248346415849 1101 20-21 0.4348768429627725 1101 22-23 0.45958815362680383 1101 24-25 0.5755230110544574 1101 26-27 0.5531676274556574 1101 28-29 0.4707010242553338 1101 30-31 0.4128135210557744 1101 32-33 0.42491125522641937 1101 34-35 0.4537205892354734 1101 36-37 0.43357038594085395 1101 38-39 0.4602567702216902 1101 40-41 0.40713232095535545 1101 42-43 0.379806688973197 1101 44-45 0.2544509995961377 1101 46-47 0.36392406808221267 1101 48-49 0.43023843651520366 1101 50-51 0.28558258578539863 1101 52-53 0.4490853001281039 1101 54-55 0.5817664632570185 1101 56-57 0.6203798230727884 1101 58-59 0.5805057373338229 1101 60-61 0.5468759719483245 1101 62-63 0.5824126177058773 1101 64-65 0.6383062706353755 1101 66-67 0.5883821955181574 1101 68-69 0.5092651382855422 1101 70-71 0.5071591590318363 1101 72-73 0.4830667876792134 1101 74-75 0.4635430697962022 1101 76-77 0.45856414381410104 1101 78-79 0.5506148860911608 1101 80-81 0.5324492760137858 1101 82-83 0.3803679942606166 1101 84-85 0.5481639335365287 1101 86-87 0.4835871468325408 1101 88-89 0.6748972167683789 1101 90-91 0.5925241863536215 1101 92-93 0.4423879121244667 1101 94-95 0.46166156599147 1101 96-97 0.49198183516481464 1101 98-99 0.37830504491335404 1101 100-101 0.5336698947585639 2304 1 0.27951339660751984 2304 2 0.2826044873345168 2304 3 0.28060430321298924 2304 4 0.30340967867340396 2304 5 0.2968038247545053 2304 6 0.3101731462749058 2304 7 0.32761056118937404 2304 8 0.3250663250482262 2304 9 0.35242285189968214 2304 10-11 0.34790184906727006 2304 12-13 0.3081634186039537 2304 14-15 0.4495126150282047 2304 16-17 0.45938962960997287 2304 18-19 0.4207905393804907 2304 20-21 0.40687326054591466 2304 22-23 0.4318668795215004 2304 24-25 0.5379399687728039 2304 26-27 0.5615424326991487 2304 28-29 0.5291449887336341 2304 30-31 0.47626763098902103 2304 32-33 0.4508167843418249 2304 34-35 0.43031199994208436 2304 36-37 0.36438102196950695 2304 38-39 0.38812006494243434 2304 40-41 0.4428464097469842 2304 42-43 0.4132150104517862 2304 44-45 0.4500876268940317 2304 46-47 0.4454994729157917 2304 48-49 0.4301916083089523 2304 50-51 0.36184426920625157 2304 52-53 0.40284888582174005 2304 54-55 0.5958167368219591 2304 56-57 0.6828271068792802 2304 58-59 0.6052527311126426 2304 60-61 0.5277569093631556 2304 62-63 0.6131712661548292 2304 64-65 0.6390355933632392 2304 66-67 0.675955312898239 2304 68-69 0.6137680503777005 2304 70-71 0.5459489433148903 2304 72-73 0.630644796617922 2304 74-75 0.7102227963730385 2304 76-77 0.6222949694556377 2304 78-79 0.6633310086999273 2304 80-81 0.4926378570206751 2304 82-83 0.5779270986248903 2304 84-85 0.5350356912050955 2304 86-87 0.5404009068421232 2304 88-89 0.552759211799632 2304 90-91 0.5755087696731707 2304 92-93 0.5238018403847562 2304 94-95 0.5949728046034366 2304 96-97 0.5216971480276484 2304 98-99 0.4471536933150517 2304 100-101 0.5270394697364686 2202 1 0.25341493725802167 2202 2 0.15795218114553222 2202 3 0.21680346894041236 2202 4 0.30378677394900677 2202 5 0.25250520546103417 2202 6 0.2894474864922856 2202 7 0.2736936396183154 2202 8 0.28878856284424614 2202 9 0.19879947730204606 2202 10-11 0.2712071142609105 2202 12-13 0.31171095461679954 2202 14-15 0.3682038791549971 2202 16-17 0.32760648821032134 2202 18-19 0.2725736642740628 2202 20-21 0.3926648222499196 2202 22-23 0.3283945631152463 2202 24-25 0.49242069007772926 2202 26-27 0.3637013874017825 2202 28-29 0.45916943424607837 2202 30-31 0.4333817606657817 2202 32-33 0.40911082402190857 2202 34-35 0.3968129684366417 2202 36-37 0.4903562565141044 2202 38-39 0.4903986474988571 2202 40-41 0.43824454753043796 2202 42-43 0.38784185600407284 2202 44-45 0.3260610732793836 2202 46-47 0.38286248960097424 2202 48-49 0.38841570345222465 2202 50-51 0.4960091011756802 2202 52-53 0.5412733882251644 2202 54-55 0.5798931099288112 2202 56-57 0.5767370284946338 2202 58-59 0.578015179933459 2202 60-61 0.58380504165833 2202 62-63 0.5621849656554971 2202 64-65 0.5471740700661414 2202 66-67 0.5941764974684602 2202 68-69 0.5912350430212143 2202 70-71 0.5291693039563512 2202 72-73 0.6414743016857116 2202 74-75 0.4705136528545495 2202 76-77 0.6122357827599814 2202 78-79 0.6312707232491874 2202 80-81 0.47521922583182175 2202 82-83 0.5616942577322774 2202 84-85 0.5639789399823343 2202 86-87 0.5501596483961677 2202 88-89 0.5026767570256183 2202 90-91 0.4119300591670694 2202 92-93 0.5008878519450732 2202 94-95 0.4580418622132427 2202 96-97 0.48003716403304963 2202 98-99 0.5565642114878457 2202 100-101 0.6208894605068167 1102 1 0.09352712364529481 1102 2 -0.03407198067441897 1102 3 0.07458395768987458 1102 4 0.047918089772650774 1102 5 0.07581437925254875 1102 6 0.01778853128710267 1102 7 0.06889755480818849 1102 8 0.1159866710871924 1102 9 0.038140232774679816 1102 10-11 0.04885535201540847 1102 12-13 0.06216114108153192 1102 14-15 0.012015516741364252 1102 16-17 0.02087860825852772 1102 18-19 0.10758529411479856 1102 20-21 0.06336007258196474 1102 22-23 0.03498686600595846 1102 24-25 0.08982137511066668 1102 26-27 0.13005600755819557 1102 28-29 0.04487375722077047 1102 30-31 0.034545057981922866 1102 32-33 -0.012084361383500664 1102 34-35 0.06406949925856509 1102 36-37 0.08028724633770423 1102 38-39 0.018887058664411427 1102 40-41 0.003985183857004415 1102 42-43 -0.0558484712945031 1102 44-45 0.04591464094090014 1102 46-47 0.07515014533945674 1102 48-49 0.00980675086689331 1102 50-51 -0.019582574090925675 1102 52-53 0.0855682166479994 1102 54-55 0.2568271262447652 1102 56-57 0.2512817447196838 1102 58-59 0.2383058234361286 1102 60-61 0.2864835215549917 1102 62-63 0.24942907498738975 1102 64-65 0.24115107123026291 1102 66-67 0.18618707595337014 1102 68-69 0.20224192980055022 1102 70-71 0.13537967877666546 1102 72-73 0.2315825013499122 1102 74-75 0.1932840779759175 1102 76-77 0.23273443069133037 1102 78-79 0.19491647898380648 1102 80-81 0.15711024818708097 1102 82-83 0.06229776217576699 1102 84-85 0.2011285924539905 1102 86-87 0.22587267381778986 1102 88-89 0.18550149840119445 1102 90-91 0.17400974464879226 1102 92-93 0.13366418311189676 1102 94-95 0.17814428682431327 1102 96-97 0.14552279246590416 1102 98-99 0.22275144622837217 1102 100-101 0.19089515013017078 2305 1 0.12251144810337422 2305 2 0.09624753246355766 2305 3 0.07588056370747864 2305 4 0.135686367221993 2305 5 0.12812807194872278 2305 6 0.20367627039775016 2305 7 0.12386110375723547 2305 8 0.18593512484911656 2305 9 0.1505089448938577 2305 10-11 0.1392453250944996 2305 12-13 0.13535867487179587 2305 14-15 0.09368492618067847 2305 16-17 0.21780451662804978 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68-69 -0.6106764694313114 1213 70-71 -0.6668454427188166 1213 72-73 -0.6626453240492154 1213 74-75 -0.6231709804592498 1213 76-77 -0.8214620700481561 1213 78-79 -0.7862742809861487 1213 80-81 -0.7383138364198771 1213 82-83 -0.5901038025085583 1213 84-85 -0.5694843022983704 1213 86-87 -0.6806257560775677 1213 88-89 -0.6152768231950674 1213 90-91 -0.6387419426478331 1213 92-93 -0.57092247612119 1213 94-95 -0.5937272826668156 1213 96-97 -0.6611948698412817 1213 98-99 -0.7507806459122293 1213 100-101 -1.1752098214310394 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3387.0 3 265.0 4 243.0 5 361.0 6 733.0 7 2633.0 8 9463.0 9 20698.0 10 30930.0 11 25089.0 12 18457.0 13 15233.0 14 14010.0 15 13845.0 16 14189.0 17 14507.0 18 15271.0 19 16872.0 20 19026.0 21 21482.0 22 24203.0 23 26533.0 24 30941.0 25 35741.0 26 42936.0 27 52203.0 28 64677.0 29 79997.0 30 99492.0 31 128806.0 32 176958.0 33 255000.0 34 389392.0 35 649475.0 36 1245855.0 37 2320638.0 38 1006128.0 39 835.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.46495889534616 26.720170525002366 21.465230322647933 16.349640257003546 2 31.819221125883356 24.670344423134377 29.89728598677625 13.613148464206018 3 36.047546001119585 21.27536695480222 25.41250881491229 17.26457822916591 4 30.918156051837958 20.828948512437094 30.131316359339067 18.12157907638588 5 29.981996659326803 19.59467545492207 32.514824291844604 17.908503593906524 6 26.52889218038429 19.717169272584687 35.48329811717672 18.270640429854303 7 32.019615184683 19.94269574289422 27.936981311774563 20.100707760648216 8 29.81836945424473 27.456084821017058 23.625318010479333 19.10022771425888 9 27.62454778753909 26.953360110862928 25.56219436950781 19.859897732090175 10-11 23.384960793342007 29.461652992094706 29.946909202707705 17.206477011855576 12-13 23.624530883531158 27.437340871186176 30.100751895005157 18.837376350277506 14-15 22.359375820148077 27.700412747585666 31.18248613718016 18.757725295086097 16-17 22.1260736929046 27.964414479445125 32.05765945887084 17.85185236877943 18-19 21.85834153478736 28.064717813645373 31.985241816955178 18.091698834612092 20-21 21.750975240222957 28.877286766418305 31.178372505276343 18.1933654880824 22-23 22.338958070399613 28.73470568106949 30.45396054607486 18.47237570245604 24-25 21.639916332867788 29.22105800743251 30.45882027476921 18.680205384930495 26-27 21.505552070740936 29.511733410880474 30.336276235610754 18.646438282767836 28-29 21.731759407763715 29.38657128383592 30.0771204478207 18.804548860579665 30-31 21.904473088540062 29.072502883561747 30.205572171498655 18.817451856399533 32-33 21.258235455318786 29.479704733644922 30.40046817196554 18.861591639070753 34-35 22.17378586885835 28.95144247282345 30.198379015262 18.676392643056193 36-37 21.415529965080708 28.756663641740598 30.768886304162418 19.058920089016272 38-39 21.220297448468045 29.271380087276466 30.583585909363652 18.924736554891837 40-41 21.44512767535221 29.651629914614624 29.723972010730865 19.1792703993023 42-43 21.56545011527218 29.015428401549396 30.215774655276434 19.20334682790199 44-45 21.053998382564615 29.192312776028167 30.467617975016047 19.28607086639117 46-47 21.423613329323008 29.39450530631089 29.82521943088305 19.35666193348305 48-49 21.022230277163136 29.533635918095236 29.79667603539175 19.647457769349874 50-51 20.963134772827615 29.19821884199088 29.89503219095853 19.943614194222974 52-53 21.807603655835475 29.053268046012715 29.301945456186495 19.837182841965312 54-55 21.86594516252961 29.257857008901667 29.215142118957143 19.661055709611578 56-57 21.837108434858248 29.17767696466716 29.372813995440538 19.612400605034054 58-59 21.59912925028948 29.23141682786603 29.45684706937408 19.712606852470405 60-61 20.815191527152358 29.297229473199742 29.381981253092714 20.505597746555186 62-63 21.097679096767813 29.186712799321135 29.499581387887215 20.216026716023837 64-65 20.689090380626244 29.389851263150053 29.77711284420126 20.143945512022444 66-67 20.610858805811016 29.082604819986074 29.65705009627737 20.649486277925536 68-69 20.44465204359786 29.06033976689227 30.46116069143044 20.033847498079428 70-71 20.300651977829652 30.079460321276237 29.616448383335094 20.003439317559017 72-73 20.31324572522456 30.463269442835607 29.1580000564913 20.06548477544853 74-75 20.41903597307509 30.465853739509573 29.06972968502373 20.045380602391603 76-77 20.43285362571915 30.40080937646649 29.098152694458634 20.06818430335572 78-79 20.309492245767988 30.316648430407746 29.369949994066847 20.003909329757423 80-81 20.162149966112278 30.614639335837047 29.240047897850925 19.983162800199754 82-83 20.10598821158659 30.425606271770405 29.22730258428209 20.241102932360917 84-85 20.225972321267925 30.02746166111727 29.46615113528838 20.28041488232642 86-87 20.364214377646938 29.989196469482266 29.52672699348008 20.119862159390713 88-89 20.61042735829651 30.303155233870267 29.065720782168412 20.020696625664808 90-91 20.635119449003874 30.131581055357398 29.04193932226545 20.19136017337328 92-93 20.398861639317147 30.275597479541826 29.00386450545987 20.321676375681157 94-95 20.15566917711855 30.30295044464948 28.990419495942106 20.55096088228986 96-97 20.27480364096661 30.581319706255677 28.820057871789263 20.323818780988447 98-99 20.557951517611322 30.52872158149078 28.648330075247262 20.264996825650634 100-101 20.6618551464354 30.35585871204189 28.362539928122825 20.61974621339989 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2777.0 1 1625.5 2 361.0 3 255.5 4 259.0 5 299.0 6 459.5 7 781.0 8 1209.0 9 1686.0 10 2168.5 11 2459.0 12 2459.5 13 2314.0 14 2105.5 15 1839.5 16 1616.5 17 1514.0 18 1549.5 19 1705.5 20 1893.5 21 2395.5 22 3349.0 23 4858.0 24 7085.5 25 10804.0 26 17454.5 27 26612.0 28 37622.0 29 51115.5 30 67857.0 31 86827.5 32 111689.5 33 146262.5 34 188123.5 35 235300.5 36 290100.5 37 348758.0 38 403004.5 39 437971.0 40 454355.0 41 448314.0 42 422715.5 43 401385.5 44 372906.5 45 344035.0 46 327898.5 47 300903.0 48 259467.0 49 220500.0 50 181123.5 51 145857.0 52 118434.0 53 93791.5 54 76147.5 55 60441.0 56 43562.0 57 30053.0 58 21205.5 59 15628.5 60 11588.5 61 8272.5 62 5680.5 63 3846.5 64 2715.5 65 1976.0 66 1410.0 67 1053.0 68 822.0 69 599.5 70 453.0 71 375.0 72 305.0 73 227.5 74 190.5 75 173.0 76 157.0 77 129.5 78 102.5 79 91.5 80 66.0 81 53.5 82 56.5 83 45.5 84 38.5 85 36.0 86 32.0 87 31.5 88 27.0 89 19.0 90 13.0 91 9.5 92 8.5 93 11.5 94 7.5 95 5.0 96 3.5 97 2.5 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17040576757088938 2 0.1640600223277297 3 0.13002243228204036 4 0.1250271545620245 5 0.13718136227031885 6 0.15787400980236127 7 0.1332896924186786 8 0.12467864681411642 9 0.11762136491897776 10-11 0.10530016391481077 12-13 0.1337979328843779 14-15 0.12974653031494646 16-17 0.11990118643654313 18-19 0.11835468330520102 20-21 0.11160960626756333 22-23 0.1246278227675465 24-25 0.425920031412165 26-27 0.30498058230997904 28-29 0.40205451125854286 30-31 0.2337107478627762 32-33 0.27505974003645395 34-35 0.288615239314462 36-37 0.28562388114491766 38-39 0.3060987113345175 40-41 0.29790877925867754 42-43 0.3555359874908952 44-45 0.300537108524151 46-47 0.3275174166747017 48-49 0.3271906906610379 50-51 0.32734316280074766 52-53 0.3309734518414569 54-55 0.30808810972882616 56-57 0.32087398773020387 58-59 0.3205472617165401 60-61 0.2987219640037964 62-63 0.29568704236576354 64-65 0.3047918072798622 66-67 0.3038406715511964 68-69 0.30071136239810503 70-71 0.2739053081215084 72-73 0.2641398306020007 74-75 0.2731719897352851 76-77 0.26865591016864293 78-79 0.2660130597470066 80-81 0.27550263529942043 82-83 0.31898623742903515 84-85 0.32511416532975224 86-87 0.3139256145062865 88-89 0.3209175511986924 90-91 0.29614445878489287 92-93 0.27968472827431745 94-95 0.26551934043747016 96-97 0.2645464229745601 98-99 0.2866839255448047 100-101 0.28737368046253947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6886504.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.44966887229201 #Duplication Level Percentage of deduplicated Percentage of total 1 92.05957097263035 62.09387578629183 2 4.382534668903685 5.912010244777869 3 1.2959554588898385 2.6223529972607653 4 0.47044127606698993 1.2692443317830793 5 0.3548520285447568 1.1967325912002478 6 0.2355014964646724 0.953069877328284 7 0.16572624837252622 0.7824726403321882 8 0.08841846681038945 0.4771037046845208 9 0.09275613098928215 0.5630733288991822 >10 0.7070228324196409 9.489337999370278 >50 0.08566732545825176 4.042951176734672 >100 0.05600572594751005 7.530872690180662 >500 0.0044882991296869845 2.0103011978937086 >1k 0.0010375049162898076 0.9194551744269145 >5k 2.1564456032013586E-5 0.13714625883586257 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 9429 0.1369199814593878 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.422270574445321E-4 0.0 0.0 2 9.874386190728997E-4 0.0 0.0 3 0.0010600443998870835 0.0 0.0 4 0.001089086712212757 0.0 0.0 5 0.0012923828984924717 0.0 0.0 6 0.0015247213970978597 0.0 0.0 7 0.0016844541148890641 0.0 2.904231232567352E-5 8 0.0022507792052396977 0.0 2.904231232567352E-5 9 0.0025847657969849435 0.0 2.904231232567352E-5 10-11 0.004385389161176701 0.0 2.904231232567352E-5 12-13 0.006556302007520797 0.0 2.904231232567352E-5 14-15 0.009431490927762475 0.0 2.904231232567352E-5 16-17 0.012880265516436206 0.0 2.904231232567352E-5 18-19 0.016278216058540008 0.0 2.904231232567352E-5 20-21 0.018187748093953043 0.0 2.904231232567352E-5 22-23 0.01980685700610934 0.0 4.3563468488510284E-5 24-25 0.021796255400417977 0.0 5.082404656992866E-5 26-27 0.023996210559087745 0.0 5.808462465134704E-5 28-29 0.026631800402642618 0.0 5.808462465134704E-5 30-31 0.045879592896482746 0.0 5.808462465134704E-5 32-33 0.05062801096173036 0.0 6.534520273276542E-5 34-35 0.05440351156406792 0.0 9.438751505843894E-5 36-37 0.05881068245948888 0.0 1.0164809313985732E-4 38-39 0.06364622746171351 0.0 1.0164809313985732E-4 40-41 0.06910618217894014 0.0 1.0164809313985732E-4 42-43 0.07556809667140249 0.0 1.089086712212757E-4 44-45 0.08232043428712159 0.0 1.3795098354694923E-4 46-47 0.08921072288638764 0.0 1.452115616283676E-4 48-49 0.09724092224443637 0.0 1.452115616283676E-4 50-51 0.1053509879613807 0.0 1.452115616283676E-4 52-53 0.11454287981245637 0.0 1.452115616283676E-4 54-55 0.1245189140963252 7.26057808141838E-6 1.452115616283676E-4 56-57 0.13535895717188284 1.452115616283676E-5 1.452115616283676E-4 58-59 0.1463224300748246 1.452115616283676E-5 1.452115616283676E-4 60-61 0.1591954350131794 1.452115616283676E-5 1.452115616283676E-4 62-63 0.17379645753491174 1.452115616283676E-5 1.5973271779120437E-4 64-65 0.19004563128112612 1.452115616283676E-5 1.7425387395404114E-4 66-67 0.20962014978863003 1.452115616283676E-5 1.7425387395404114E-4 68-69 0.23026197327410253 1.452115616283676E-5 1.7425387395404114E-4 70-71 0.2537572039455724 1.452115616283676E-5 1.7425387395404114E-4 72-73 0.28005501775646974 1.452115616283676E-5 1.7425387395404114E-4 74-75 0.3091191118163875 1.452115616283676E-5 1.7425387395404114E-4 76-77 0.34176267087044454 1.452115616283676E-5 1.8877503011687787E-4 78-79 0.37857380174323574 1.452115616283676E-5 2.0329618627971463E-4 80-81 0.42046007669493835 1.452115616283676E-5 2.1055676436113302E-4 82-83 0.4691640344650929 2.178173424425514E-5 2.178173424425514E-4 84-85 0.5230738267196244 2.904231232567352E-5 2.178173424425514E-4 86-87 0.5842078941651672 2.904231232567352E-5 2.178173424425514E-4 88-89 0.6518547001497421 2.904231232567352E-5 2.2507792052396978E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1655 0.0 57.285095 9 AGAGCGG 1725 0.0 56.333267 8 CGGGAGA 2165 0.0 54.305584 4 GATCGGG 2940 0.0 51.931244 1 GAGAGCG 2265 0.0 48.76307 7 ATCGGGA 3145 0.0 45.377518 2 CGCCGGA 925 0.0 40.576683 58-59 GGCGCCG 1575 0.0 39.364697 56-57 GATCCGG 630 0.0 39.13659 1 TTGTGTT 54800 0.0 38.771675 1 GCGACCG 50 0.0016310455 37.93018 2 AGCGGCG 1545 0.0 36.511234 10-11 AAGCGGC 870 0.0 36.506077 9 TCGGGAG 3305 0.0 36.14736 3 CGCCGTA 1825 0.0 35.665546 58-59 GGGCGCC 2470 0.0 35.29498 54-55 TGTGTTT 97270 0.0 35.072933 1 CGAGGCG 245 0.0 34.835865 1 CGGAAAG 1260 0.0 34.61529 5 GTCGCCG 1880 0.0 34.49465 56-57 >>END_MODULE