FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_35010-1.342000000357e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_35010-1.342000000357e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6886504
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT94290.1369199814593878Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC16550.057.2850959
AGAGCGG17250.056.3332678
CGGGAGA21650.054.3055844
GATCGGG29400.051.9312441
GAGAGCG22650.048.763077
ATCGGGA31450.045.3775182
CGCCGGA9250.040.57668358-59
GGCGCCG15750.039.36469756-57
GATCCGG6300.039.136591
TTGTGTT548000.038.7716751
GCGACCG500.001631045537.930182
AGCGGCG15450.036.51123410-11
AAGCGGC8700.036.5060779
TCGGGAG33050.036.147363
CGCCGTA18250.035.66554658-59
GGGCGCC24700.035.2949854-55
TGTGTTT972700.035.0729331
CGAGGCG2450.034.8358651
CGGAAAG12600.034.615295
GTCGCCG18800.034.4946556-57
Produced by FastQC (version 0.11.2)