##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_35008-2.342000000358b0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5804081 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58184318930077 33.0 33.0 33.0 27.0 33.0 2 31.35413478895281 33.0 33.0 33.0 27.0 33.0 3 31.25031697524552 33.0 33.0 33.0 27.0 33.0 4 34.82910903552173 37.0 37.0 37.0 33.0 37.0 5 34.85887309291514 37.0 37.0 37.0 33.0 37.0 6 35.164159321691066 37.0 37.0 37.0 33.0 37.0 7 34.78569027551476 37.0 37.0 37.0 33.0 37.0 8 34.97125539771068 37.0 37.0 37.0 33.0 37.0 9 34.665570656233086 37.0 37.0 37.0 33.0 37.0 10-11 34.656750999856826 37.0 37.0 37.0 33.0 37.0 12-13 34.60347891423294 37.0 37.0 37.0 33.0 37.0 14-15 36.218154346915554 40.0 37.0 40.0 30.0 40.0 16-17 36.16501285905555 40.0 37.0 40.0 27.0 40.0 18-19 36.187810094311224 40.0 37.0 40.0 27.0 40.0 20-21 36.163668804759965 40.0 37.0 40.0 27.0 40.0 22-23 36.50101161579241 40.0 37.0 40.0 33.0 40.0 24-25 36.02991455494849 40.0 37.0 40.0 27.0 40.0 26-27 35.95988150406585 40.0 37.0 40.0 27.0 40.0 28-29 35.69954830058367 40.0 37.0 40.0 27.0 40.0 30-31 35.71229889796507 40.0 37.0 40.0 27.0 40.0 32-33 35.60234695208423 40.0 37.0 40.0 27.0 40.0 34-35 35.588970846547454 40.0 37.0 40.0 27.0 40.0 36-37 35.50262823692502 37.0 37.0 40.0 27.0 40.0 38-39 35.3807388284209 37.0 37.0 40.0 27.0 40.0 40-41 35.33261751515873 37.0 37.0 40.0 27.0 40.0 42-43 35.195429388390686 37.0 37.0 40.0 24.5 40.0 44-45 35.062581397468435 37.0 37.0 40.0 22.0 40.0 46-47 34.9410564738845 37.0 37.0 40.0 22.0 40.0 48-49 34.82299609533361 37.0 37.0 40.0 22.0 40.0 50-51 33.83394054976145 37.0 35.0 38.5 22.0 38.5 52-53 34.27101129360531 37.0 35.0 38.5 22.0 40.0 54-55 34.91282883543493 37.0 37.0 40.0 22.0 40.0 56-57 35.09188388308158 37.0 37.0 40.0 24.5 40.0 58-59 34.77599787115307 37.0 37.0 40.0 22.0 40.0 60-61 34.67140723914777 37.0 33.0 40.0 22.0 40.0 62-63 34.41415281764675 37.0 33.0 40.0 22.0 40.0 64-65 34.222943994062106 37.0 33.0 40.0 22.0 40.0 66-67 34.0557727399049 37.0 33.0 40.0 22.0 40.0 68-69 33.99034084810326 37.0 33.0 40.0 22.0 40.0 70-71 33.65337010286383 37.0 33.0 37.0 22.0 40.0 72-73 33.668888408001195 37.0 33.0 37.0 22.0 40.0 74-75 33.56408068736464 37.0 33.0 37.0 22.0 40.0 76-77 33.246553588759355 37.0 33.0 37.0 22.0 40.0 78-79 32.85710718027539 37.0 33.0 37.0 22.0 37.0 80-81 32.42688067240964 37.0 33.0 37.0 22.0 37.0 82-83 32.08876400932378 37.0 33.0 37.0 15.0 37.0 84-85 31.83575487661182 37.0 33.0 37.0 15.0 37.0 86-87 31.64749354807419 35.0 33.0 37.0 15.0 37.0 88-89 31.449862260709317 33.0 33.0 37.0 15.0 37.0 90-91 31.26840709149304 33.0 33.0 37.0 15.0 37.0 92-93 31.095913547726163 33.0 33.0 37.0 15.0 37.0 94-95 30.919883785219398 33.0 33.0 37.0 15.0 37.0 96-97 30.77713500207871 33.0 30.0 37.0 15.0 37.0 98-99 30.603163704986198 33.0 27.0 37.0 15.0 37.0 100-101 29.32470342850143 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19877321370043077 2306 2 0.07920425162582134 2306 3 0.08917040904720608 2306 4 0.23923808011852543 2306 5 0.1917110990354658 2306 6 0.10095885724446418 2306 7 0.4048742645738628 2306 8 0.05956305045388888 2306 9 0.21147384657401602 2306 10-11 0.17555941397490926 2306 12-13 0.24209880482593604 2306 14-15 0.5576037488486847 2306 16-17 0.4751289283935023 2306 18-19 0.40894866523535 2306 20-21 0.3665954354168619 2306 22-23 0.33182933441919715 2306 24-25 0.607066824266397 2306 26-27 0.4239066913483356 2306 28-29 0.33331925059548695 2306 30-31 0.4922707690695063 2306 32-33 0.44216347029106373 2306 34-35 0.4851800861491995 2306 36-37 0.31199080815909497 2306 38-39 0.3579909990348753 2306 40-41 0.26253137871060517 2306 42-43 0.3208243035124241 2306 44-45 0.385991836087598 2306 46-47 0.2426255789896956 2306 48-49 0.3123803512001757 2306 50-51 0.28712089864712453 2306 52-53 0.2401243063972771 2306 54-55 0.5262696863999565 2306 56-57 0.4478184978006112 2306 58-59 0.664939880879281 2306 60-61 0.5751511224085846 2306 62-63 0.5163231721960386 2306 64-65 0.5204145646524623 2306 66-67 0.39932995731378185 2306 68-69 0.34312794719650697 2306 70-71 0.47995937820815016 2306 72-73 0.26155622261726563 2306 74-75 0.4293509056198559 2306 76-77 0.47868243923021225 2306 78-79 0.4614144418730248 2306 80-81 0.4885417582512872 2306 82-83 0.5129506374309187 2306 84-85 0.4506369224038842 2306 86-87 0.4292372216514586 2306 88-89 0.3753437223650913 2306 90-91 0.29497314507394634 2306 92-93 0.13926334440431987 2306 94-95 0.26390796063122934 2306 96-97 0.31631561972703537 2306 98-99 0.5033333451182145 2306 100-101 0.4155477355629955 2307 1 0.12488869026891436 2307 2 0.010207657234108325 2307 3 0.05106112177192301 2307 4 0.09503064766142444 2307 5 0.07551700746513035 2307 6 0.06664670371133496 2307 7 0.3124143763992606 2307 8 0.025431656533974945 2307 9 0.1581816053570435 2307 10-11 0.19154603579375618 2307 12-13 0.16535249493527715 2307 14-15 0.43368394019517353 2307 16-17 0.3499315898473583 2307 18-19 0.31465508142027687 2307 20-21 0.27830358946791733 2307 22-23 0.17717336599021394 2307 24-25 0.37071194265377017 2307 26-27 0.3107611208321259 2307 28-29 0.3966137754373662 2307 30-31 0.34441886037142666 2307 32-33 0.43161024081997823 2307 34-35 0.3294766902847286 2307 36-37 0.3492957053066803 2307 38-39 0.23510328640760036 2307 40-41 0.3110778757560695 2307 42-43 0.29593732259060346 2307 44-45 0.2510215612935909 2307 46-47 0.13540893035303014 2307 48-49 0.15792267337486976 2307 50-51 0.24005406315767885 2307 52-53 0.16274586313347328 2307 54-55 0.21868314043055648 2307 56-57 0.2803986879652385 2307 58-59 0.1656775941500186 2307 60-61 0.10962926224837588 2307 62-63 0.17055577048842707 2307 64-65 0.29916200755724276 2307 66-67 0.20942606128424046 2307 68-69 0.17992644165868654 2307 70-71 0.04233034158269078 2307 72-73 -0.06171662026330438 2307 74-75 0.13162672364739336 2307 76-77 0.24749640928912697 2307 78-79 0.2378337429813726 2307 80-81 0.21040533930605676 2307 82-83 0.3132955054993758 2307 84-85 0.2748678962891482 2307 86-87 0.08399725981781003 2307 88-89 0.18514738052469326 2307 90-91 0.23114047791825243 2307 92-93 0.3624824912399589 2307 94-95 0.1887709562690958 2307 96-97 0.24432227651331573 2307 98-99 0.08674977009358642 2307 100-101 0.260603654826415 2201 1 0.2709950666089007 2201 2 0.2457260120602065 2201 3 0.26064332487706565 2201 4 0.3838702295776173 2201 5 0.36048394642264725 2201 6 0.3561602981298648 2201 7 0.49263513402374315 2201 8 0.38369135816569155 2201 9 0.3616990707448622 2201 10-11 0.38217160738337697 2201 12-13 0.37186138393840196 2201 14-15 0.5616487643458186 2201 16-17 0.5045131533698708 2201 18-19 0.5169335198344456 2201 20-21 0.5332610721663116 2201 22-23 0.40359655689785967 2201 24-25 0.5508230447323541 2201 26-27 0.5231853123578389 2201 28-29 0.48278202392537395 2201 30-31 0.571721985479769 2201 32-33 0.490694137324752 2201 34-35 0.32853844647104324 2201 36-37 0.49920825583072315 2201 38-39 0.46496372589052015 2201 40-41 0.424574653335533 2201 42-43 0.31910736908288584 2201 44-45 0.41498619058073416 2201 46-47 0.34896437618994725 2201 48-49 0.3754211162601422 2201 50-51 0.34558137214065 2201 52-53 0.34807114768796765 2201 54-55 0.5452429151061864 2201 56-57 0.5316651512406381 2201 58-59 0.5972066569044259 2201 60-61 0.4298514305410919 2201 62-63 0.4607962774682548 2201 64-65 0.4351178189724223 2201 66-67 0.49762588124471563 2201 68-69 0.4989083576091673 2201 70-71 0.43904847572289185 2201 72-73 0.4315687782444968 2201 74-75 0.4036400520712746 2201 76-77 0.3862419068067027 2201 78-79 0.48954588178156655 2201 80-81 0.5769624301255902 2201 82-83 0.47616695656293473 2201 84-85 0.4286305250574536 2201 86-87 0.4370646861731551 2201 88-89 0.5094387887182883 2201 90-91 0.6017693191499305 2201 92-93 0.4999095519214052 2201 94-95 0.3313557726969236 2201 96-97 0.4484457912769173 2201 98-99 0.3438449830267807 2201 100-101 0.3169493904839271 1101 1 0.29032263591625806 1101 2 0.1605461554715717 1101 3 0.22443132441851787 1101 4 0.23085344047613177 1101 5 0.23845566236217053 1101 6 0.27096075031501954 1101 7 0.2720789199609186 1101 8 0.23709944868170396 1101 9 0.2531498972552839 1101 10-11 0.230671234676187 1101 12-13 0.2364997263211066 1101 14-15 0.45998315537408274 1101 16-17 0.3516463521739155 1101 18-19 0.4447272468350647 1101 20-21 0.4344210444559806 1101 22-23 0.3926562158282678 1101 24-25 0.5120731523758906 1101 26-27 0.49560517208389854 1101 28-29 0.34614350821600226 1101 30-31 0.4397729160958974 1101 32-33 0.4377331308891783 1101 34-35 0.3350773048296958 1101 36-37 0.3828827757679534 1101 38-39 0.3703397597484681 1101 40-41 0.33620520205157334 1101 42-43 0.146656299586013 1101 44-45 0.21221606288395378 1101 46-47 0.33106688904272374 1101 48-49 0.3446653680950149 1101 50-51 0.3273929320422866 1101 52-53 0.3788570360447494 1101 54-55 0.5730300052311179 1101 56-57 0.6526416824803078 1101 58-59 0.6684572746549478 1101 60-61 0.5315857668288402 1101 62-63 0.5354646942223553 1101 64-65 0.5992367024195531 1101 66-67 0.549117477917946 1101 68-69 0.5403586356174657 1101 70-71 0.6003483325089931 1101 72-73 0.5246012380516376 1101 74-75 0.496047932510983 1101 76-77 0.652432455347224 1101 78-79 0.6215791462413165 1101 80-81 0.4700861881110967 1101 82-83 0.4622035734676615 1101 84-85 0.5927283460230761 1101 86-87 0.5839485633371986 1101 88-89 0.6191514174860124 1101 90-91 0.3979923335311071 1101 92-93 0.4546765838722031 1101 94-95 0.4829965345224956 1101 96-97 0.5101697499044988 1101 98-99 0.5345363534977317 1101 100-101 0.47987029044426777 2304 1 0.2746692891436844 2304 2 0.2437382603685414 2304 3 0.25110627421679865 2304 4 0.2961283916294519 2304 5 0.24794664064553018 2304 6 0.34315527813221536 2304 7 0.3393779397961296 2304 8 0.3422020696987289 2304 9 0.303440371462834 2304 10-11 0.32720391543495 2304 12-13 0.30071847395955587 2304 14-15 0.41161535618720535 2304 16-17 0.4579215279515978 2304 18-19 0.40879920688841054 2304 20-21 0.38226780374154146 2304 22-23 0.4390314771299728 2304 24-25 0.5156823862609201 2304 26-27 0.5465569852089516 2304 28-29 0.5364359316000744 2304 30-31 0.5166168813880461 2304 32-33 0.40334955275352513 2304 34-35 0.5508119020354982 2304 36-37 0.4689477531830448 2304 38-39 0.5198505633459973 2304 40-41 0.4926353738449052 2304 42-43 0.5053652098764729 2304 44-45 0.49533152970391825 2304 46-47 0.45865193229202816 2304 48-49 0.5583875447727564 2304 50-51 0.46734067951864944 2304 52-53 0.37962748194232887 2304 54-55 0.6535938748989665 2304 56-57 0.7598525932825453 2304 58-59 0.6962933655866337 2304 60-61 0.6076134753633653 2304 62-63 0.587448294038694 2304 64-65 0.6175997954395811 2304 66-67 0.5894105788232693 2304 68-69 0.6838482296727904 2304 70-71 0.6621620621123512 2304 72-73 0.6576676366978447 2304 74-75 0.751175204878173 2304 76-77 0.6143588880050004 2304 78-79 0.6012693783131908 2304 80-81 0.5458470835648725 2304 82-83 0.625202574384403 2304 84-85 0.6118855460674801 2304 86-87 0.625823077020268 2304 88-89 0.6670988407945195 2304 90-91 0.5682641081520998 2304 92-93 0.5829832914889508 2304 94-95 0.6445472034704949 2304 96-97 0.5654183506928163 2304 98-99 0.6117596525154845 2304 100-101 0.5852294574892198 2202 1 0.2054284803709301 2202 2 0.11514808828335532 2202 3 0.10984703234918314 2202 4 0.18925580280163956 2202 5 0.0925858970607436 2202 6 0.16241266011713407 2202 7 0.1311217179360682 2202 8 0.15237019572879973 2202 9 0.06173250194925828 2202 10-11 0.1428356770753254 2202 12-13 0.19563072800848147 2202 14-15 0.20813279488933745 2202 16-17 0.17016913174133208 2202 18-19 0.193206691535174 2202 20-21 0.22311179457103236 2202 22-23 0.23097114143035924 2202 24-25 0.349078444598625 2202 26-27 0.2810611469266746 2202 28-29 0.30074160608376843 2202 30-31 0.353584466947261 2202 32-33 0.36617307566598356 2202 34-35 0.27394637984119896 2202 36-37 0.3387291479109109 2202 38-39 0.2538226558981833 2202 40-41 0.2702614223307549 2202 42-43 0.25754562310189755 2202 44-45 0.29107209908583087 2202 46-47 0.35279951674635157 2202 48-49 0.31240390161243425 2202 50-51 0.2894480785371485 2202 52-53 0.42768162570573764 2202 54-55 0.5208229949534768 2202 56-57 0.42786986164888674 2202 58-59 0.42424995758177175 2202 60-61 0.4062387043959532 2202 62-63 0.44472991895007397 2202 64-65 0.4356878452328701 2202 66-67 0.474989927680177 2202 68-69 0.5065459342828973 2202 70-71 0.4788477189179474 2202 72-73 0.47927219985559333 2202 74-75 0.43158635864995887 2202 76-77 0.5135806375837575 2202 78-79 0.4738820301192135 2202 80-81 0.47408795218533584 2202 82-83 0.4754111206374745 2202 84-85 0.3900208755521355 2202 86-87 0.3891544998305285 2202 88-89 0.4326465854565633 2202 90-91 0.2633052649623089 2202 92-93 0.3951064810957021 2202 94-95 0.2769411579309917 2202 96-97 0.3046953673304813 2202 98-99 0.2733709140131495 2202 100-101 0.41219695097502296 1102 1 0.05640823466249145 1102 2 -0.0657604216751082 1102 3 0.008958118248344249 1102 4 -0.08126223325967885 1102 5 -0.05610093350416179 1102 6 0.01345770820763903 1102 7 -0.11317109465220909 1102 8 0.058783420578720325 1102 9 0.0345200377798065 1102 10-11 0.005015826216862251 1102 12-13 -0.04064086486692986 1102 14-15 -0.1261846726682876 1102 16-17 -0.06481954603607676 1102 18-19 -0.006858467199762686 1102 20-21 0.01582401099450692 1102 22-23 0.09065079276455634 1102 24-25 0.046631837329250914 1102 26-27 0.1595863367494843 1102 28-29 -0.0362183501400537 1102 30-31 0.04178788028478664 1102 32-33 -0.07979746340146932 1102 34-35 0.09121479523942355 1102 36-37 0.08978135439367918 1102 38-39 -0.04449757236771035 1102 40-41 0.009319482101588505 1102 42-43 -0.022272052068402104 1102 44-45 -0.10645705930334515 1102 46-47 0.0033942228548013986 1102 48-49 -0.08495295369620237 1102 50-51 -0.06159695025849743 1102 52-53 0.025964672780567355 1102 54-55 0.26098595546384473 1102 56-57 0.3373124756544854 1102 58-59 0.29270651173626305 1102 60-61 0.2680691728287954 1102 62-63 0.2740406603202601 1102 64-65 0.27427181105164067 1102 66-67 0.3247794786656826 1102 68-69 0.2672431192403195 1102 70-71 0.25994861790396584 1102 72-73 0.4268090954827599 1102 74-75 0.34321594831779123 1102 76-77 0.4111053774504825 1102 78-79 0.33268058176999915 1102 80-81 0.23544388288438967 1102 82-83 0.18289814291608764 1102 84-85 0.3216125778371115 1102 86-87 0.30349789195915733 1102 88-89 0.2283231866596367 1102 90-91 0.2402316635693751 1102 92-93 0.22817649750405167 1102 94-95 0.30176626933950246 1102 96-97 0.2878274360016526 1102 98-99 0.35337428289532724 1102 100-101 0.3002807361939297 2305 1 0.20704607279552434 2305 2 0.18559424917429368 2305 3 0.14970322235635436 2305 4 0.2249510500304126 2305 5 0.21234886328764446 2305 6 0.26635955107382614 2305 7 0.10393044070979585 2305 8 0.2711199257340695 2305 9 0.12671224154473748 2305 10-11 0.2240712593567622 2305 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2821.0 3 286.0 4 352.0 5 438.0 6 980.0 7 5112.0 8 19088.0 9 51021.0 10 74740.0 11 59945.0 12 39798.0 13 28474.0 14 22497.0 15 19366.0 16 18351.0 17 18940.0 18 19568.0 19 21117.0 20 22691.0 21 25198.0 22 27465.0 23 30181.0 24 34069.0 25 38616.0 26 45540.0 27 54143.0 28 65039.0 29 79251.0 30 97149.0 31 123467.0 32 163503.0 33 228692.0 34 339287.0 35 546248.0 36 970755.0 37 1664921.0 38 838601.0 39 6371.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.46145766510494 26.21357179087711 20.799003976190832 16.525966567827112 2 30.336241957392453 26.814260225067894 28.887291234763634 13.962206582776018 3 34.74506986829577 22.658430041736903 26.24706527970349 16.34943481026383 4 29.222699643463628 21.943737359588358 30.482531649762183 18.351031347185835 5 32.13257381597433 20.20129958970949 30.392069752664575 17.274056841651603 6 29.092802899899723 20.597885161914 33.28327258225641 17.02603935592987 7 32.77765816272662 21.697227162093807 26.913248494058006 18.61186618112157 8 28.347645900290967 29.92713157941495 24.078061207679223 17.647161312614855 9 30.38528976726335 27.271646837118844 24.078139486746043 18.264923908871765 10-11 26.68541493853403 29.882940725456535 27.534640768169204 15.897003567840231 12-13 27.665119402121174 27.317150931916995 26.886328718280623 18.131400947681204 14-15 25.465127663033936 27.55272803963353 28.27360658799134 18.708537709341197 16-17 25.938484719503585 27.761276931748252 28.405176484590992 17.89506186415717 18-19 25.247545289616795 28.364140205588058 28.759006929551784 17.62930757524336 20-21 24.157939718731107 29.383923327621254 28.476573718167085 17.981563235480554 22-23 25.778673660068733 28.54133486173842 27.504977882546555 18.175013595646288 24-25 22.953209468665733 30.875496025309296 28.064621056993495 18.10667344903148 26-27 22.664957339256066 31.0171895073036 27.793535807865883 18.524317345574453 28-29 23.157306826268094 30.802424740573308 27.644246153441678 18.396022279716927 30-31 24.359952732314483 28.619949867658594 28.43620783449714 18.58388956552978 32-33 24.35873406713986 28.837788036105643 28.141705999117967 18.661771897636527 34-35 22.114766561587498 28.77597612581028 28.206418412848745 20.90283889975347 36-37 21.049478683924605 28.458997939129603 28.415919891243263 22.075603485702526 38-39 20.706272097246288 29.970808005179705 29.365990790369718 19.956929107204292 40-41 21.59716000238786 28.609440804629127 30.697305908889344 19.09609328409367 42-43 22.416469454553564 28.561630017109866 29.835396388999964 19.186504139336602 44-45 21.1822181744135 29.601787025765127 30.166375132861923 19.04961966695945 46-47 22.601172983021577 29.911075691867794 28.047184101022964 19.440567224087665 48-49 20.65517039046499 29.74931301380298 29.48748094633553 20.1080356493965 50-51 20.375197386687965 28.41352909725852 30.446129129630243 20.76514438642327 52-53 23.438343806380686 28.507449672033296 27.995932056564392 20.058274465021622 54-55 23.568890885684414 28.468770789953428 28.726674212249765 19.23566411211239 56-57 24.017530679726452 28.189660669929197 28.228796126307028 19.564012524037327 58-59 22.87198779460145 28.80194259121385 28.05530726759037 20.27076234659433 60-61 20.992139519787354 28.294316311349554 28.232943970871638 22.480600197991453 62-63 22.5709314150829 28.54458380717699 28.136179816281697 20.748304961458413 64-65 20.776988834559944 29.46512414516152 29.89310604165776 19.86478097862078 66-67 20.418735218612053 29.1369354172898 28.6880270304035 21.756302333694645 68-69 20.146234921192228 28.82665365694863 31.254787823035237 19.772323598823906 70-71 20.03149250206901 30.42064972567787 29.787897627170995 19.75996014508213 72-73 20.057066286121124 32.278948242040926 27.85755036243543 19.806435109402525 74-75 19.808111120563858 32.37138707667221 28.277901599189153 19.542600203574782 76-77 20.12962118006731 32.045766859964445 27.88460068563232 19.940011274335927 78-79 19.96415195467193 32.376956515806285 27.975336807361405 19.683554722160377 80-81 19.91760081915496 31.734127037252808 28.324692503790665 20.023579639801568 82-83 20.107453285247196 31.766826872186893 27.856111069682704 20.269608772883206 84-85 19.94744475641805 31.43529097827864 28.46377405721503 20.15349020808828 86-87 20.28483431179874 30.901451129940693 28.246171499374213 20.56754305888635 88-89 20.247139335789814 31.339237247229722 28.154663118995437 20.258960297985027 90-91 20.30918773857785 30.752869759062396 28.411301521282876 20.526640981076877 92-93 20.40991616866601 30.87644660315269 28.002243011190696 20.711394216990602 94-95 20.171222320858803 30.778451057135335 28.519184892003878 20.531141730001977 96-97 20.44286116612657 30.512608569825435 28.16406051298252 20.880469751065476 98-99 20.3920805417942 30.80444269031596 28.13037862310531 20.673098144784532 100-101 20.51153519297652 30.42486295063977 28.26532161832183 20.798280238061878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2308.0 1 1362.5 2 358.5 3 323.5 4 337.0 5 401.5 6 572.5 7 807.0 8 1109.5 9 1450.0 10 1795.0 11 2107.0 12 2254.0 13 2266.5 14 2215.0 15 2140.0 16 2103.0 17 2211.5 18 2549.5 19 3135.0 20 4173.0 21 5822.0 22 8145.0 23 11322.0 24 15644.0 25 21498.5 26 28619.0 27 37377.5 28 48124.0 29 60185.5 30 73968.5 31 89876.0 32 108199.5 33 129377.5 34 153082.0 35 179193.5 36 207882.0 37 237217.0 38 264145.0 39 284009.0 40 296587.5 41 300849.0 42 296130.5 43 287441.5 44 276181.5 45 265154.5 46 255652.5 47 243387.5 48 226538.0 49 208433.0 50 189620.0 51 169963.0 52 148632.0 53 126222.5 54 105643.5 55 86986.0 56 70934.0 57 57590.0 58 46073.5 59 36019.0 60 27857.0 61 21320.0 62 15983.5 63 11792.0 64 8645.5 65 6280.0 66 4555.0 67 3369.0 68 2470.5 69 1845.0 70 1389.0 71 1079.0 72 869.5 73 675.5 74 535.0 75 448.0 76 364.0 77 300.0 78 266.0 79 213.5 80 185.0 81 176.5 82 139.0 83 111.0 84 101.0 85 93.0 86 87.0 87 71.0 88 55.0 89 51.0 90 52.0 91 37.5 92 28.0 93 21.5 94 16.0 95 14.5 96 13.0 97 11.5 98 6.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16957034197145077 2 0.1632299755982041 3 0.1297707595741686 4 0.12399895866374022 5 0.13686921323117301 6 0.15716527732814203 7 0.13282033796564865 8 0.1239472708943931 9 0.11640085656971363 10-11 0.10398717729818037 12-13 0.1330529329277107 14-15 0.12876284807189975 16-17 0.11894217189594701 18-19 0.11730539253328823 20-21 0.11071520194153045 22-23 0.12385250998392337 24-25 0.42015264776628714 26-27 0.3064998576001955 28-29 0.39074230700777607 30-31 0.2206981604839767 32-33 0.2615401128964258 34-35 0.2740399384502043 36-37 0.27136078907237854 38-39 0.2907953903468956 40-41 0.2837744683439118 42-43 0.33941635204608617 44-45 0.28579029134844947 46-47 0.3126937752936253 48-49 0.3122027414848277 50-51 0.31299528728148346 52-53 0.3162688460068011 54-55 0.2934314665835987 56-57 0.3061294285865411 58-59 0.30649124297197095 60-61 0.28355048801007426 62-63 0.2809574849144938 64-65 0.2895118107414421 66-67 0.2897960934728513 68-69 0.2862210227596755 70-71 0.25988610427731795 72-73 0.24978975999818057 74-75 0.25936061195562227 76-77 0.25544957074169017 78-79 0.2523052314397404 80-81 0.26168656157624265 82-83 0.30426005426182023 84-85 0.3097648016972885 86-87 0.29950477948188525 88-89 0.30581930197045837 90-91 0.2825598057642545 92-93 0.26591634403448194 94-95 0.25138346621971674 96-97 0.25062537893595904 98-99 0.2727649734729753 100-101 0.27287696363989405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5804081.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.0811511089395 #Duplication Level Percentage of deduplicated Percentage of total 1 96.74811379220823 88.11929571812992 2 2.3116339810387267 4.210925678710953 3 0.3513194413177588 0.9599573736651298 4 0.15751823312736918 0.5738776797554829 5 0.10965367397588342 0.49936914245239117 6 0.06172283336458807 0.3373072027531163 7 0.0352945684251421 0.2250268944038621 8 0.041205450499824894 0.3002431890789182 9 0.024434914126128065 0.20030040952702474 >10 0.13738579156116734 2.3258279819607623 >50 0.013734202777718287 0.8623194089034265 >100 0.007928779004755472 1.1929837495101145 >500 3.543228836862497E-5 0.017781946996820758 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.890628443223881E-5 0.17478362415205537 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 10150 0.17487695295775507 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017573841578020707 0.0 0.0 2 0.0019813644916395895 0.0 0.0 3 0.002257032594824228 0.0 0.0 4 0.0025154714415598265 0.0 0.0 5 0.0028945150834387046 0.0 0.0 6 0.003566456084951261 0.0 3.4458512898079816E-5 7 0.004169480060667658 0.0 5.1687769347119725E-5 8 0.004893108831527334 0.0 5.1687769347119725E-5 9 0.00565119611528509 0.0 5.1687769347119725E-5 10-11 0.00776178003029248 0.0 5.1687769347119725E-5 12-13 0.009674227496135907 0.0 5.1687769347119725E-5 14-15 0.011629748103101939 0.0 5.1687769347119725E-5 16-17 0.01324929820931169 0.0 5.1687769347119725E-5 18-19 0.015411569893666198 0.0 5.1687769347119725E-5 20-21 0.017970114476348623 0.0 7.753165402067959E-5 22-23 0.02107999526540033 0.0 8.614628224519954E-5 24-25 0.023931437207716434 0.0 8.614628224519954E-5 26-27 0.027506507920892213 0.0 8.614628224519954E-5 28-29 0.031219412685660315 0.0 8.614628224519954E-5 30-31 0.04729430895261455 0.0 1.0337553869423945E-4 32-33 0.05207542761722313 0.0 1.2060479514327936E-4 34-35 0.056675639089116776 8.614628224519954E-6 1.4644867981683922E-4 36-37 0.06199086470364559 1.7229256449039908E-5 1.6367793626587913E-4 38-39 0.06745253899799125 1.7229256449039908E-5 1.89521820939439E-4 40-41 0.07287975477943881 1.7229256449039908E-5 1.9813644916395894E-4 42-43 0.0799092914106471 1.7229256449039908E-5 2.239803338375188E-4 44-45 0.08714557911924385 1.7229256449039908E-5 2.584388467355986E-4 46-47 0.09487290063663825 1.7229256449039908E-5 2.584388467355986E-4 48-49 0.10345307034826012 1.7229256449039908E-5 2.584388467355986E-4 50-51 0.11288608825410948 1.7229256449039908E-5 2.9289735963367844E-4 52-53 0.12332701766222766 1.7229256449039908E-5 3.187412443072383E-4 54-55 0.13389716649371364 2.5843884673559862E-5 3.2735587253175826E-4 56-57 0.14560444625083627 3.4458512898079816E-5 3.6181438542983807E-4 58-59 0.15861253486986138 3.4458512898079816E-5 3.6181438542983807E-4 60-61 0.17309372491527944 3.4458512898079816E-5 3.6181438542983807E-4 62-63 0.18765244661471817 5.1687769347119725E-5 3.876582701033979E-4 64-65 0.20408915726710225 5.1687769347119725E-5 3.962728983279179E-4 66-67 0.2218697499225114 5.1687769347119725E-5 4.221167830014777E-4 68-69 0.24104591235029282 5.1687769347119725E-5 4.307314112259977E-4 70-71 0.2630218289510432 5.1687769347119725E-5 4.307314112259977E-4 72-73 0.28720309037727076 5.1687769347119725E-5 4.393460394505177E-4 74-75 0.3148215884650817 5.1687769347119725E-5 4.738045523485975E-4 76-77 0.3455844258548425 5.1687769347119725E-5 4.8241918057311743E-4 78-79 0.37870767137812167 5.1687769347119725E-5 4.910338087976375E-4 80-81 0.41650004539909075 5.1687769347119725E-5 4.996484370221574E-4 82-83 0.4588409431226063 5.1687769347119725E-5 5.168776934711972E-4 84-85 0.5053168623938915 5.1687769347119725E-5 5.68565462818317E-4 86-87 0.5568754123176434 5.1687769347119725E-5 5.68565462818317E-4 88-89 0.6152136746540926 5.1687769347119725E-5 5.944093474918768E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4650 0.0 86.37572 2 GAGCGGC 3010 0.0 81.59308 9 GATCGGG 5015 0.0 81.41996 1 AGAGCGG 3495 0.0 71.76266 8 AAGCGGC 1160 0.0 69.89211 9 TCGGGAG 4625 0.0 69.715355 3 CGGGAGA 4795 0.0 67.83701 4 GATCCGG 745 0.0 64.293015 1 GAGAGCG 4375 0.0 62.855564 7 TCGGGAA 1810 0.0 59.991283 3 GAGCGTC 9730 0.0 59.39924 9 TCGGAGA 1190 0.0 58.97202 4 AGAGCGT 10180 0.0 57.472145 8 GTTCGGA 605 0.0 56.433674 2 AAAGCGG 1575 0.0 55.69051 8 AAGAGCG 10425 0.0 55.21237 7 TTCGGAG 860 0.0 52.930256 3 TCGGAAG 11245 0.0 52.79297 3 GGATCGG 1380 0.0 52.578846 1 CGGAAGA 11420 0.0 51.485725 4 >>END_MODULE