FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_35008-2.342000000358b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_35008-2.342000000358b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5804081
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT101500.17487695295775507Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA46500.086.375722
GAGCGGC30100.081.593089
GATCGGG50150.081.419961
AGAGCGG34950.071.762668
AAGCGGC11600.069.892119
TCGGGAG46250.069.7153553
CGGGAGA47950.067.837014
GATCCGG7450.064.2930151
GAGAGCG43750.062.8555647
TCGGGAA18100.059.9912833
GAGCGTC97300.059.399249
TCGGAGA11900.058.972024
AGAGCGT101800.057.4721458
GTTCGGA6050.056.4336742
AAAGCGG15750.055.690518
AAGAGCG104250.055.212377
TTCGGAG8600.052.9302563
TCGGAAG112450.052.792973
GGATCGG13800.052.5788461
CGGAAGA114200.051.4857254
Produced by FastQC (version 0.11.2)