##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2404685 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78642608075486 33.0 33.0 33.0 27.0 33.0 2 31.724133098513942 33.0 33.0 33.0 27.0 33.0 3 31.649453046864767 33.0 33.0 33.0 27.0 33.0 4 35.358950548616555 37.0 37.0 37.0 33.0 37.0 5 35.35532304646971 37.0 37.0 37.0 33.0 37.0 6 35.45271626013386 37.0 37.0 37.0 33.0 37.0 7 35.30745856525907 37.0 37.0 37.0 33.0 37.0 8 35.31668804853858 37.0 37.0 37.0 33.0 37.0 9 35.2071556149766 37.0 37.0 37.0 33.0 37.0 10-11 35.143379694221906 37.0 37.0 37.0 33.0 37.0 12-13 35.10418765867463 37.0 37.0 37.0 33.0 37.0 14-15 36.72610071589418 40.0 37.0 40.0 33.0 40.0 16-17 36.68599795815253 40.0 37.0 40.0 33.0 40.0 18-19 36.728401225108485 40.0 37.0 40.0 33.0 40.0 20-21 36.715133375057434 40.0 37.0 40.0 33.0 40.0 22-23 36.79784108937345 40.0 37.0 40.0 33.0 40.0 24-25 36.48875154126216 40.0 37.0 40.0 33.0 40.0 26-27 36.38843757082529 40.0 37.0 40.0 33.0 40.0 28-29 36.28218519265517 40.0 37.0 40.0 33.0 40.0 30-31 36.343312949513134 40.0 37.0 40.0 33.0 40.0 32-33 36.24730224540845 40.0 37.0 40.0 33.0 40.0 34-35 36.18465682615394 40.0 37.0 40.0 33.0 40.0 36-37 36.102613232086526 38.5 37.0 40.0 33.0 40.0 38-39 36.038713178649175 37.0 37.0 40.0 30.0 40.0 40-41 35.95884658489574 37.0 37.0 40.0 27.0 40.0 42-43 35.77916670998488 37.0 37.0 40.0 27.0 40.0 44-45 35.66907557538721 37.0 37.0 40.0 27.0 40.0 46-47 35.567636925418505 37.0 37.0 40.0 27.0 40.0 48-49 35.44294637343353 37.0 37.0 40.0 27.0 40.0 50-51 34.304544462164486 37.0 35.0 38.5 24.5 38.5 52-53 34.76108118111104 37.0 35.0 38.5 27.0 40.0 54-55 35.49671037994581 37.0 37.0 40.0 27.0 40.0 56-57 35.575595348247276 37.0 37.0 40.0 27.0 40.0 58-59 35.33760388574803 37.0 37.0 40.0 27.0 40.0 60-61 35.1016752713973 37.0 35.0 40.0 27.0 40.0 62-63 34.88487660545976 37.0 33.0 40.0 27.0 40.0 64-65 34.61827682212015 37.0 33.0 40.0 27.0 40.0 66-67 34.365656208609444 37.0 33.0 37.0 27.0 40.0 68-69 34.16285355462358 37.0 33.0 37.0 27.0 40.0 70-71 33.85364236895893 37.0 33.0 37.0 27.0 40.0 72-73 33.64464846747079 37.0 33.0 37.0 27.0 38.5 74-75 33.4161987952684 37.0 33.0 37.0 27.0 37.0 76-77 33.1641824189031 37.0 33.0 37.0 27.0 37.0 78-79 32.91861761519701 37.0 33.0 37.0 24.5 37.0 80-81 32.68463936024885 37.0 33.0 37.0 22.0 37.0 82-83 32.49327708202945 37.0 33.0 37.0 22.0 37.0 84-85 32.304104903552854 35.0 33.0 37.0 22.0 37.0 86-87 32.16180061005912 33.0 33.0 37.0 22.0 37.0 88-89 32.03092255326581 33.0 33.0 37.0 22.0 37.0 90-91 31.886942780447335 33.0 33.0 37.0 22.0 37.0 92-93 31.72017457588 33.0 33.0 37.0 22.0 37.0 94-95 31.57834082218669 33.0 33.0 37.0 22.0 37.0 96-97 31.443672040204852 33.0 33.0 37.0 15.0 37.0 98-99 31.283374121766467 33.0 33.0 37.0 15.0 37.0 100-101 30.014560742883162 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3096931992586427 2306 2 0.22599661593795517 2306 3 0.23677482876992073 2306 4 0.40110660066849846 2306 5 0.363317782056356 2306 6 0.3524873651167013 2306 7 0.45542037691546966 2306 8 0.2633267619539197 2306 9 0.2669143711000146 2306 10-11 0.31886592273620096 2306 12-13 0.271027233719515 2306 14-15 0.6083371737982617 2306 16-17 0.4957942139602949 2306 18-19 0.4404904971540091 2306 20-21 0.33662256690757886 2306 22-23 0.43168900364724294 2306 24-25 0.5879156219502732 2306 26-27 0.5387879533460591 2306 28-29 0.39729203643813094 2306 30-31 0.40791345616599983 2306 32-33 0.43122833363284485 2306 34-35 0.3121393264936998 2306 36-37 0.2663579845736095 2306 38-39 0.39001795402893435 2306 40-41 0.30875100747017825 2306 42-43 0.39916222365035736 2306 44-45 0.4138177222197754 2306 46-47 0.24450424131130433 2306 48-49 0.35940327396705385 2306 50-51 0.4246249361619192 2306 52-53 0.5082887750937246 2306 54-55 0.6575291213677517 2306 56-57 0.681511069398212 2306 58-59 0.7534576342097097 2306 60-61 0.6435462812470405 2306 62-63 0.550744286741164 2306 64-65 0.5668713733851973 2306 66-67 0.49353205211561857 2306 68-69 0.5407112344360954 2306 70-71 0.4697064948438552 2306 72-73 0.4729862585037381 2306 74-75 0.41692399665821256 2306 76-77 0.39081585958313525 2306 78-79 0.34379630825596763 2306 80-81 0.23654563335737322 2306 82-83 0.28411983493959525 2306 84-85 0.4221017086393104 2306 86-87 0.20913301940618823 2306 88-89 0.38745431704870015 2306 90-91 0.30566933433099663 2306 92-93 0.11881767912722196 2306 94-95 0.25263806690985646 2306 96-97 0.20367465798712558 2306 98-99 0.22434639051988725 2306 100-101 0.04622327153689554 2307 1 0.20130304491041429 2307 2 0.2413455255563619 2307 3 0.18192321638952436 2307 4 0.38146538268753716 2307 5 0.2802086506722361 2307 6 0.2182729848935523 2307 7 0.2875514841019253 2307 8 0.3929746427770908 2307 9 0.3819107015099661 2307 10-11 0.3109038719944053 2307 12-13 0.37465072921044396 2307 14-15 0.572030776890891 2307 16-17 0.5731751899158724 2307 18-19 0.4807441787018405 2307 20-21 0.4721849007363872 2307 22-23 0.4660006607573308 2307 24-25 0.6073362684744623 2307 26-27 0.5239679277731781 2307 28-29 0.4593266923573225 2307 30-31 0.4791042647949695 2307 32-33 0.5464680847526893 2307 34-35 0.4781307723426451 2307 36-37 0.595666904343183 2307 38-39 0.48415367798315145 2307 40-41 0.40314111977578193 2307 42-43 0.4668924367302978 2307 44-45 0.40614646630600504 2307 46-47 0.1995026372937403 2307 48-49 0.26629546174648056 2307 50-51 0.33778970090752836 2307 52-53 0.3983735092639904 2307 54-55 0.6215861799133293 2307 56-57 0.5476503956133456 2307 58-59 0.4547904768426321 2307 60-61 0.41122467222091075 2307 62-63 0.3595461541649243 2307 64-65 0.6063274636708229 2307 66-67 0.5543163969041487 2307 68-69 0.40455756193657777 2307 70-71 0.5129072730589499 2307 72-73 0.4320201372436685 2307 74-75 0.55480476803681 2307 76-77 0.5715525377830843 2307 78-79 0.6353977149169268 2307 80-81 0.4841829444207093 2307 82-83 0.4354204296599633 2307 84-85 0.6049166025745976 2307 86-87 0.5360423106132188 2307 88-89 0.5789194812297254 2307 90-91 0.5207111195218168 2307 92-93 0.5727944088385293 2307 94-95 0.3932764384831948 2307 96-97 0.4272692232770403 2307 98-99 0.367725758497361 2307 100-101 0.492163766597951 2201 1 0.34890870337047275 2201 2 0.31657497755927366 2201 3 0.3666160169524275 2201 4 0.40013576465210576 2201 5 0.39464988868341067 2201 6 0.4692382347755739 2201 7 0.4451833407988559 2201 8 0.5030437098359357 2201 9 0.4540290778140559 2201 10-11 0.4520461016557036 2201 12-13 0.46843399252656326 2201 14-15 0.5986619103167357 2201 16-17 0.5113212031434884 2201 18-19 0.5759334439897046 2201 20-21 0.5933963545673961 2201 22-23 0.4514388682121364 2201 24-25 0.6741501891705042 2201 26-27 0.5575195142843086 2201 28-29 0.5964111130755327 2201 30-31 0.6095248000829159 2201 32-33 0.3097034127019782 2201 34-35 0.5330303588605148 2201 36-37 0.6508794500947133 2201 38-39 0.5810460531122743 2201 40-41 0.505914912112253 2201 42-43 0.48773010697816943 2201 44-45 0.6182900420707611 2201 46-47 0.5602712685382656 2201 48-49 0.5075422823556082 2201 50-51 0.5666904130125303 2201 52-53 0.5492552098320047 2201 54-55 0.6309616305549852 2201 56-57 0.638303786037774 2201 58-59 0.629480365243424 2201 60-61 0.5406314215914847 2201 62-63 0.5030714189949279 2201 64-65 0.3961303793118276 2201 66-67 0.43441513547222854 2201 68-69 0.579466143051512 2201 70-71 0.5573838161261264 2201 72-73 0.5615868030312612 2201 74-75 0.3690037747594701 2201 76-77 0.35875751303532155 2201 78-79 0.434015251519547 2201 80-81 0.5531135132013176 2201 82-83 0.46848693637279837 2201 84-85 0.4232230764961642 2201 86-87 0.429602504555362 2201 88-89 0.5030942704346373 2201 90-91 0.5675848657911367 2201 92-93 0.4118623822935241 2201 94-95 0.4381558586992078 2201 96-97 0.5212178738349813 2201 98-99 0.405460000978902 2201 100-101 0.2538937760638831 1101 1 0.2743420811421551 1101 2 0.19305660209256814 1101 3 0.24666672651249044 1101 4 0.16072621331335313 1101 5 0.3494971993324185 1101 6 0.15560832734855978 1101 7 0.2677367395022543 1101 8 0.23173718072050775 1101 9 0.32910642962045245 1101 10-11 0.282925981049182 1101 12-13 0.2639698069320744 1101 14-15 0.4318888200238149 1101 16-17 0.4152897142094716 1101 18-19 0.412925154367926 1101 20-21 0.38076714071093676 1101 22-23 0.48422071950562895 1101 24-25 0.5292629878315367 1101 26-27 0.5359936486708747 1101 28-29 0.5905497669672073 1101 30-31 0.3019548096766158 1101 32-33 0.36263644784150273 1101 34-35 0.3740975749845816 1101 36-37 0.3784705279531977 1101 38-39 0.30084951722727027 1101 40-41 0.2563419044663604 1101 42-43 0.21997734827011328 1101 44-45 0.053577209354166655 1101 46-47 0.36490189030142517 1101 48-49 0.43741196792311143 1101 50-51 0.24034443966723273 1101 52-53 0.36721578013804645 1101 54-55 0.5595505986299756 1101 56-57 0.6090372888182856 1101 58-59 0.549872377968569 1101 60-61 0.6310276497305125 1101 62-63 0.7439745350787064 1101 64-65 0.5932220033052147 1101 66-67 0.6021673231266718 1101 68-69 0.41625383155111706 1101 70-71 0.5483850496019116 1101 72-73 0.40273656235812183 1101 74-75 0.4440373630941963 1101 76-77 0.501311181691328 1101 78-79 0.43880872738785826 1101 80-81 0.4833551623210681 1101 82-83 0.28929550674529736 1101 84-85 0.34870551296385344 1101 86-87 0.47905704453534526 1101 88-89 0.4986299290982501 1101 90-91 0.24610400958450995 1101 92-93 0.2937819945585183 1101 94-95 0.311668264060053 1101 96-97 0.2097221494690551 1101 98-99 0.04981248848840636 1101 100-101 0.3227950273980724 2304 1 0.17587940452475692 2304 2 0.11316822266392279 2304 3 0.10744181065472702 2304 4 0.11624667572981906 2304 5 0.06809629205960732 2304 6 0.244860071165931 2304 7 0.05734049866847357 2304 8 0.10955818132210027 2304 9 0.02964746013928732 2304 10-11 0.19434798485387716 2304 12-13 0.14053985229570998 2304 14-15 0.32244293933506896 2304 16-17 0.17727490192388728 2304 18-19 0.07050689834790091 2304 20-21 0.0698778344982216 2304 22-23 0.07894882219979849 2304 24-25 0.303522983278917 2304 26-27 0.2976516165748748 2304 28-29 0.3174045545385411 2304 30-31 0.21012392337838293 2304 32-33 0.2821363096368614 2304 34-35 0.20945824844766037 2304 36-37 0.0494134087882756 2304 38-39 0.21168821615252398 2304 40-41 0.18749668553395082 2304 42-43 0.0807666593606271 2304 44-45 0.11739015343819403 2304 46-47 0.1557586371267874 2304 48-49 0.10477473884485988 2304 50-51 0.052508073961497814 2304 52-53 0.06587580485228983 2304 54-55 0.25071302160121434 2304 56-57 0.4060725960390741 2304 58-59 0.31780646248477495 2304 60-61 0.486733875443079 2304 62-63 0.4856548189672907 2304 64-65 0.4825769941186735 2304 66-67 0.41293163543664235 2304 68-69 0.4034749833648519 2304 70-71 0.4532037169839924 2304 72-73 0.3077035964865331 2304 74-75 0.5023505754578181 2304 76-77 0.5298067428834727 2304 78-79 0.47345439507886766 2304 80-81 0.2644664495753446 2304 82-83 0.31822803849171066 2304 84-85 0.31713591473286584 2304 86-87 0.31587191535465564 2304 88-89 0.3696087837882587 2304 90-91 0.3769055558505521 2304 92-93 0.3217196561360005 2304 94-95 0.3690292870995826 2304 96-97 0.3754254484951183 2304 98-99 0.3802445405834227 2304 100-101 0.44482769247132126 2202 1 0.22728061854071413 2202 2 0.2145579318478532 2202 3 0.1999751280893456 2202 4 0.29140308555149375 2202 5 0.298781681947645 2202 6 0.22499909797824813 2202 7 0.18442673926199404 2202 8 0.19984153555355277 2202 9 0.21550476109637628 2202 10-11 0.10667056823363197 2202 12-13 0.19117194267245452 2202 14-15 0.35377031748166843 2202 16-17 0.3806148897390145 2202 18-19 0.277511664236215 2202 20-21 0.37186313998645204 2202 22-23 0.2687186976122504 2202 24-25 0.10116148841463968 2202 26-27 0.24795458491470157 2202 28-29 0.28052399891571156 2202 30-31 0.2501504916220654 2202 32-33 0.3105586012503565 2202 34-35 0.2484286544797314 2202 36-37 0.390270257936173 2202 38-39 0.27785637115009365 2202 40-41 0.2022808604744668 2202 42-43 0.29848454317953355 2202 44-45 0.20775397162645248 2202 46-47 0.25853509777466854 2202 48-49 0.17668948757373215 2202 50-51 0.3292711312471752 2202 52-53 0.3138204223608696 2202 54-55 0.4282549373839828 2202 56-57 0.4285276293798006 2202 58-59 0.5005674731213858 2202 60-61 0.44987067022482563 2202 62-63 0.42592622587317663 2202 64-65 0.3567359070564393 2202 66-67 0.5359218996471995 2202 68-69 0.4176612097985739 2202 70-71 0.4526842528576438 2202 72-73 0.359524713582438 2202 74-75 0.2799332938027632 2202 76-77 0.44513572052776595 2202 78-79 0.4709149479442729 2202 80-81 0.3572725992108232 2202 82-83 0.291297532334994 2202 84-85 0.35241743843841533 2202 86-87 0.389265873408128 2202 88-89 0.3867651898836826 2202 90-91 0.34158094724773136 2202 92-93 0.5601743010139693 2202 94-95 0.36086539773973314 2202 96-97 0.2767729111905872 2202 98-99 0.2528582195026594 2202 100-101 0.31155725532496703 1102 1 0.11875082919308255 1102 2 0.05089065522006564 1102 3 0.12575978586647096 1102 4 0.016514836693815482 1102 5 0.02187822210888868 1102 6 0.07857011314203532 1102 7 0.1801343580219097 1102 8 0.2284356309631974 1102 9 -0.004373854355065987 1102 10-11 0.06079996603381943 1102 12-13 0.07940859978973691 1102 14-15 0.005422657389054564 1102 16-17 0.059845148919386304 1102 18-19 -0.018840910769043262 1102 20-21 0.05753116437790595 1102 22-23 0.13321305167391273 1102 24-25 0.2823045141471425 1102 26-27 0.3275558219916732 1102 28-29 0.10142653433466364 1102 30-31 0.13512667329233352 1102 32-33 -0.003769139450696457 1102 34-35 0.11363306136480134 1102 36-37 0.22238188767781253 1102 38-39 0.06925420521673686 1102 40-41 0.08220913324122847 1102 42-43 0.04461837512952371 1102 44-45 0.06528926353055198 1102 46-47 0.0764610982110625 1102 48-49 -0.025629285609859664 1102 50-51 0.10898354472902838 1102 52-53 0.16330636508958918 1102 54-55 0.3796449550213623 1102 56-57 0.4753795201449975 1102 58-59 0.4607798113832118 1102 60-61 0.4643455840444588 1102 62-63 0.2952598692286301 1102 64-65 0.26166021715975774 1102 66-67 0.2766474522320479 1102 68-69 0.29409649053059894 1102 70-71 0.24601078479509653 1102 72-73 0.33587336020683267 1102 74-75 0.24599827422337484 1102 76-77 0.3288720826131666 1102 78-79 0.19926136176410125 1102 80-81 0.1781127201597812 1102 82-83 0.16932130670235068 1102 84-85 0.22487341859162058 1102 86-87 0.37661418809644687 1102 88-89 0.14097428460969041 1102 90-91 0.14127496509805937 1102 92-93 0.35805134188647614 1102 94-95 0.32891846556722015 1102 96-97 0.23857243228304625 1102 98-99 0.29910633345635773 1102 100-101 0.3886473939193742 2305 1 0.2583557652609336 2305 2 0.32147930794100077 2305 3 0.18572680656559015 2305 4 0.2859399229096624 2305 5 0.3558481456887961 2305 6 0.2129428406940903 2305 7 0.2610631335592828 2305 8 0.35538033430311344 2305 9 0.2815086930984023 2305 10-11 0.2714007482314358 2305 12-13 0.24739599918019195 2305 14-15 0.21473221363164186 2305 16-17 0.27099131862377845 2305 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7161.0 20 7988.0 21 8980.0 22 10113.0 23 11526.0 24 13089.0 25 15594.0 26 18319.0 27 21452.0 28 25828.0 29 31747.0 30 40041.0 31 52334.0 32 71526.0 33 102407.0 34 154938.0 35 257678.0 36 490491.0 37 772025.0 38 186777.0 39 153.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.05472910376635 24.93051806530302 26.27241917101554 14.742333659915088 2 29.24674794546795 22.97647857580213 35.00843472357016 12.76833875515976 3 38.244845527168856 19.83110942701708 26.112373024977565 15.8116720208365 4 28.00402714576044 18.715235576784952 36.43022174700348 16.850515530451126 5 31.886638610600066 17.640966743316973 34.15970470337497 16.31268994270799 6 24.35724960140479 18.430884339914666 41.15097803686235 16.060888021818194 7 32.16932569700129 18.806188793513392 31.862142942208667 17.162342567276646 8 27.459885822875147 27.83257907813068 27.86901149890474 16.838523600089434 9 30.760865492303914 25.684445869260202 25.38376348428516 18.170925154150723 10-11 24.78980524749009 28.63040483694582 28.728274394434717 17.851515521129375 12-13 25.605211923330213 25.4548471607356 28.82248067712863 20.11746023880556 14-15 24.102421994908337 26.07912560678955 30.05210332890013 19.766349069401983 16-17 23.16255876033569 27.495788074959254 31.104376842229293 18.237276322475765 18-19 22.11793972386013 27.895352044507128 31.262905349494403 18.72380288213834 20-21 22.080175318850962 28.543746553686923 30.541645408548252 18.834432718913867 22-23 22.825377778351434 27.824630403220336 30.50751975964083 18.842472058787404 24-25 22.437116008195165 28.17755273711895 30.504109587897076 18.881221666788804 26-27 22.010107032940766 28.55281645271966 30.37891050013984 19.05816601419973 28-29 21.445902833509532 28.596499100928707 30.145615109824455 19.811982955737307 30-31 21.619449463363992 28.658198485829423 29.90450427510537 19.817847775701214 32-33 20.668224523106236 28.467123461859963 31.612089577440127 19.252562437593674 34-35 20.478331219643287 29.313215090391783 31.105122496382265 19.103331193582665 36-37 20.946880714895062 30.09421524405651 30.620600599248316 18.338303441800115 38-39 21.903912323712305 29.10126091590259 29.77837582087567 19.216450939509443 40-41 21.463095367417903 28.56158118418674 29.433048147319536 20.542275301075822 42-43 21.859342819852927 28.665866387612116 30.397356492190813 19.077434300344144 44-45 20.931201887600135 29.29545900472713 30.908430500772425 18.864908606900308 46-47 21.26606450977787 29.215753465370696 30.574975915879627 18.943206108971808 48-49 21.15672635587566 29.777092016153773 29.778093128013634 19.28808849995693 50-51 20.733283513947725 29.459149598521524 29.85100277329767 19.956564114233082 52-53 21.39818533206688 28.94758687375456 29.773035107412976 19.88119268676559 54-55 22.06455221102823 29.092619396108333 29.27918901126445 19.563639381598986 56-57 22.277534872517318 28.998209580257623 28.723087655488623 20.001167891736436 58-59 21.770962831528415 28.669302355748787 29.724891870636426 19.83484294208637 60-61 20.591255582520173 29.095014029408272 29.84076095126832 20.472969436803233 62-63 21.369175301409804 29.13631579474109 29.473024906758116 20.021483997090986 64-65 20.93942196314835 29.197648317299922 29.65612009887994 20.206809620671788 66-67 21.054270096241765 28.845672248362348 29.337651058701113 20.76240659669477 68-69 21.20233877060035 28.710450466267417 30.08449174734922 20.00271901578301 70-71 20.65659633527433 30.32687441867058 29.526427323973138 19.49010192208195 72-73 20.287089836674614 31.364006051280814 28.812825913955585 19.536078198088987 74-75 21.021631233024056 30.646373011091814 28.572301098759073 19.75969465712505 76-77 21.446464922706596 29.771436146906865 29.134512324713587 19.64758660567295 78-79 21.351204655036856 29.63121222555818 29.745874774572723 19.271708344832245 80-81 20.417408604575826 30.442148438167717 29.43216787065245 19.70827508660401 82-83 19.613777351598248 30.244222280522987 29.70047494733119 20.44152542054757 84-85 19.675076285974747 29.60302511873833 30.194479963343525 20.5274186319434 86-87 19.640707324219463 29.785173051276733 30.956914700052447 19.617204924451357 88-89 19.983920532812228 30.29229801863842 30.349691914488936 19.37408953406042 90-91 20.357883341590064 29.661486611588444 29.859898317128458 20.120731729693034 92-93 19.704387696114004 30.5805989754614 29.711702850867365 20.003310477557235 94-95 19.277408785026665 31.128784317975988 29.767810746842947 19.825996150154403 96-97 19.90841330320741 31.576233314713065 29.191665068658757 19.323688313420764 98-99 20.43990770956993 30.50942157533768 28.73574744761755 20.31492326747484 100-101 20.355745357574786 30.298064640680217 28.31429579885823 21.031894202886768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1028.0 1 602.5 2 178.5 3 193.5 4 178.0 5 173.0 6 252.5 7 352.0 8 458.5 9 612.5 10 765.0 11 882.0 12 900.5 13 844.0 14 787.0 15 706.5 16 666.0 17 666.0 18 711.0 19 814.5 20 946.5 21 1137.0 22 1445.5 23 1926.5 24 2515.5 25 3351.5 26 4613.0 27 6280.0 28 8240.5 29 10140.0 30 12340.0 31 15101.0 32 20593.0 33 30990.0 34 46656.0 35 69729.5 36 101630.5 37 140667.0 38 180831.5 39 200387.5 40 196237.5 41 174896.0 42 149620.5 43 140471.5 44 133249.0 45 124549.5 46 122737.5 47 109971.5 48 82809.0 49 62520.5 50 50379.5 51 41638.5 52 34966.5 53 27780.0 54 20931.0 55 15468.5 56 11297.5 57 8028.0 58 6085.0 59 4692.0 60 3609.5 61 2764.0 62 2068.5 63 1581.5 64 1215.0 65 919.5 66 708.5 67 561.0 68 437.5 69 327.0 70 271.5 71 211.0 72 163.5 73 138.0 74 111.5 75 87.5 76 77.5 77 60.5 78 45.5 79 35.5 80 35.0 81 31.0 82 20.0 83 19.5 84 18.5 85 13.5 86 8.5 87 8.0 88 5.0 89 4.5 90 5.0 91 2.0 92 0.5 93 1.0 94 2.0 95 2.5 96 2.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16887866809997984 2 0.16197547703753298 3 0.1301625784666183 4 0.12409109717073129 5 0.1373568679473611 6 0.15607033769495798 7 0.1323666093480019 8 0.12384158424076336 9 0.11706314964330049 10-11 0.1042964047266066 12-13 0.13272008599878984 14-15 0.12937245418838642 16-17 0.11949590071048807 18-19 0.11737504080576043 20-21 0.11067977718495353 22-23 0.12251084861426757 24-25 0.40997053668151956 26-27 0.30130765568047374 28-29 0.3808191093635965 30-31 0.21431081409831226 32-33 0.25325562391747775 34-35 0.2674154826931594 36-37 0.263943094417772 38-39 0.28546358462750837 40-41 0.2769385595202698 42-43 0.33355720187883237 44-45 0.2794336888199494 46-47 0.30600681586153694 48-49 0.305674131954913 50-51 0.30600681586153694 52-53 0.30947920413692437 54-55 0.287792371973876 56-57 0.29951947968237 58-59 0.299810578100666 60-61 0.27806136770512563 62-63 0.2753375182196421 64-65 0.28392492155937266 66-67 0.2835090666760927 68-69 0.2810555228647411 70-71 0.2531724529408218 72-73 0.24344144867207138 74-75 0.2524239141509179 76-77 0.24818219434146258 78-79 0.24564547955345503 80-81 0.2546695305206295 82-83 0.29758575447511837 84-85 0.3032205881435614 86-87 0.29348958387481106 88-89 0.3001432620072899 90-91 0.27616922798620197 92-93 0.2594726544225127 94-95 0.24483456253105917 96-97 0.24389888904367935 98-99 0.2655857212067277 100-101 0.26600157609000763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2404685.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.342263891570035 #Duplication Level Percentage of deduplicated Percentage of total 1 88.41355342017427 38.32043563928618 2 5.000599153676932 4.334745762692547 3 1.7826545448158755 2.3179285112674903 4 1.098305197489669 1.9041213481232069 5 0.6143571799041916 1.331381550754412 6 0.4402886161836744 1.1449863234652204 7 0.33651954990386285 1.0209863395623915 8 0.25550517947400975 0.8859338331540395 9 0.2004808209374276 0.7820363381641728 >10 1.4419394906479621 12.475999210571908 >50 0.19990975426752478 6.087067077043 >100 0.18382003644296904 17.165487108775892 >500 0.02504558214480904 7.234358978811476 >1k 0.006924622027138671 4.766088734555002 >5k 9.685190978289823E-5 0.22844324377302652 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5442 0.22630822748093823 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4592 0.19096056240214415 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4185 0.17403526865265095 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3719 0.15465643109180618 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3501 0.14559079463630373 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3437 0.14292932338331216 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2903 0.12072267261616387 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2802 0.11652253829503656 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2691 0.11190654909062933 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2682 0.1115322796956774 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2523 0.10492018705152649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010812226965278198 0.0 0.0 2 0.0011228081848558126 0.0 0.0 3 0.0011228081848558126 0.0 0.0 4 0.0012475646498397918 0.0 0.0 5 0.0014554920914797572 0.0 4.158548832799306E-5 6 0.0016634195331197226 0.0 4.158548832799306E-5 7 0.0019129324630876808 0.0 4.158548832799306E-5 8 0.001996103439743667 0.0 4.158548832799306E-5 9 0.0021624453930556395 0.0 4.158548832799306E-5 10-11 0.0036387302286993934 0.0 4.158548832799306E-5 12-13 0.005260564273491123 0.0 4.158548832799306E-5 14-15 0.007090325759922817 0.0 6.237823249198959E-5 16-17 0.008317097665598613 0.0 8.317097665598612E-5 18-19 0.010292408361178283 0.0 8.317097665598612E-5 20-21 0.011893449661806017 0.0 8.317097665598612E-5 22-23 0.01366083291574572 0.0 8.317097665598612E-5 24-25 0.015864863797129355 0.0 8.317097665598612E-5 26-27 0.018484749561792916 0.0 8.317097665598612E-5 28-29 0.021790795883868362 0.0 8.317097665598612E-5 30-31 0.033829794754822354 0.0 8.317097665598612E-5 32-33 0.03757248870434173 0.0 8.317097665598612E-5 34-35 0.04162707381632105 0.0 8.317097665598612E-5 36-37 0.04509946209170848 0.0 8.317097665598612E-5 38-39 0.049320389156999773 0.0 8.317097665598612E-5 40-41 0.05464333166298289 0.0 8.317097665598612E-5 42-43 0.06048609277306591 0.0 8.317097665598612E-5 44-45 0.06668233053393688 0.0 8.317097665598612E-5 46-47 0.0742924748979596 0.0 8.317097665598612E-5 48-49 0.08082139656545452 0.0 8.317097665598612E-5 50-51 0.08791172232537733 0.0 8.317097665598612E-5 52-53 0.09506442631779213 0.0 8.317097665598612E-5 54-55 0.1036310369133587 0.0 8.317097665598612E-5 56-57 0.11280063708968119 0.0 8.317097665598612E-5 58-59 0.12278115428839953 0.0 8.317097665598612E-5 60-61 0.1337181377186617 0.0 8.317097665598612E-5 62-63 0.14438481547479193 0.0 8.317097665598612E-5 64-65 0.1564653998340739 0.0 8.317097665598612E-5 66-67 0.1696479996340477 0.0 8.317097665598612E-5 68-69 0.18370389468890935 0.0 8.317097665598612E-5 70-71 0.19938162378856275 0.0 8.317097665598612E-5 72-73 0.21599502637559598 0.0 8.317097665598612E-5 74-75 0.23398075007745295 0.0 8.317097665598612E-5 76-77 0.2535051368474457 0.0 8.317097665598612E-5 78-79 0.2771672797060738 0.0 8.317097665598612E-5 80-81 0.3017443033079177 0.0 8.317097665598612E-5 82-83 0.3288372489536051 0.0 1.0396372081998266E-4 84-85 0.3591114844563841 0.0 1.247564649839792E-4 86-87 0.3939185381869143 0.0 1.247564649839792E-4 88-89 0.4321356019603399 0.0 1.247564649839792E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 580 0.0 74.39623 9 CGGGAGA 825 0.0 70.70089 4 AGAGCGG 625 0.0 69.79548 8 AACCGGA 80 0.0 65.208405 2 GAGAGCG 820 0.0 61.29436 7 CGGAAAG 270 0.0 57.959408 5 CCGAGCG 25 0.0050520776 56.9139 1 ATTCGGA 170 0.0 55.79329 2 TCGGGAG 1015 0.0 54.195805 3 ACCGGAA 135 0.0 52.69037 3 TTGTGTT 30405 0.0 52.396473 1 CGGACGG 40 5.429676E-4 47.428246 1 TGCCGGC 90 2.2919266E-10 47.424294 2 AAGCGGC 160 0.0 47.41738 9 ATCGGGA 1325 0.0 46.5295 2 TTCGGAG 195 0.0 46.2054 3 GATCCGG 155 0.0 45.898304 1 CGGAGAG 460 0.0 44.328636 5 TCGGGAA 205 0.0 43.951477 3 GAACCGG 130 0.0 43.779922 1 >>END_MODULE