FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404685
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54420.22630822748093823No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG45920.19096056240214415No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC41850.17403526865265095No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA37190.15465643109180618No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35010.14559079463630373No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG34370.14292932338331216No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA29030.12072267261616387No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC28020.11652253829503656No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT26910.11190654909062933No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA26820.1115322796956774No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG25230.10492018705152649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC5800.074.396239
CGGGAGA8250.070.700894
AGAGCGG6250.069.795488
AACCGGA800.065.2084052
GAGAGCG8200.061.294367
CGGAAAG2700.057.9594085
CCGAGCG250.005052077656.91391
ATTCGGA1700.055.793292
TCGGGAG10150.054.1958053
ACCGGAA1350.052.690373
TTGTGTT304050.052.3964731
CGGACGG405.429676E-447.4282461
TGCCGGC902.2919266E-1047.4242942
AAGCGGC1600.047.417389
ATCGGGA13250.046.52952
TTCGGAG1950.046.20543
GATCCGG1550.045.8983041
CGGAGAG4600.044.3286365
TCGGGAA2050.043.9514773
GAACCGG1300.043.7799221
Produced by FastQC (version 0.11.2)