##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_35005-1.3420000003578b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2624215 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.027097627290445 33.0 33.0 33.0 33.0 33.0 2 31.986384880811976 33.0 33.0 33.0 33.0 33.0 3 31.939998056561677 33.0 33.0 33.0 33.0 33.0 4 35.705556518806574 37.0 37.0 37.0 33.0 37.0 5 35.701959252576486 37.0 37.0 37.0 33.0 37.0 6 35.76327473168166 37.0 37.0 37.0 33.0 37.0 7 35.66812513456405 37.0 37.0 37.0 33.0 37.0 8 35.63073452441969 37.0 37.0 37.0 33.0 37.0 9 35.5727129827396 37.0 37.0 37.0 33.0 37.0 10-11 35.503114455179926 37.0 37.0 37.0 33.0 37.0 12-13 35.472444330971356 37.0 37.0 37.0 33.0 37.0 14-15 37.18328490615289 40.0 37.0 40.0 33.0 40.0 16-17 37.17115384981795 40.0 37.0 40.0 33.0 40.0 18-19 37.198831841141065 40.0 37.0 40.0 33.0 40.0 20-21 37.16385871584455 40.0 37.0 40.0 33.0 40.0 22-23 37.186733556511186 40.0 37.0 40.0 33.0 40.0 24-25 36.933988640412466 40.0 37.0 40.0 33.0 40.0 26-27 36.846297083127716 40.0 37.0 40.0 33.0 40.0 28-29 36.75144681361855 40.0 37.0 40.0 33.0 40.0 30-31 36.80196935083444 40.0 37.0 40.0 33.0 40.0 32-33 36.6979813010748 40.0 37.0 40.0 33.0 40.0 34-35 36.65816482262315 40.0 37.0 40.0 33.0 40.0 36-37 36.57593299329514 40.0 37.0 40.0 33.0 40.0 38-39 36.51250545401196 40.0 37.0 40.0 33.0 40.0 40-41 36.4008453956707 40.0 37.0 40.0 33.0 40.0 42-43 36.27078231013846 38.5 37.0 40.0 33.0 40.0 44-45 36.17695044041742 37.0 37.0 40.0 33.0 40.0 46-47 36.054598422766425 37.0 37.0 40.0 30.0 40.0 48-49 35.926975495529135 37.0 37.0 40.0 27.0 40.0 50-51 34.773198270720954 37.0 35.0 38.5 27.0 38.5 52-53 35.20740221361436 37.0 37.0 38.5 27.0 40.0 54-55 35.94508319630823 37.0 37.0 40.0 27.0 40.0 56-57 36.01459788927355 37.0 37.0 40.0 27.0 40.0 58-59 35.79871371057631 37.0 37.0 40.0 27.0 40.0 60-61 35.57761463904444 37.0 37.0 40.0 27.0 40.0 62-63 35.33707489668339 37.0 37.0 40.0 27.0 40.0 64-65 35.06384232999201 37.0 35.0 40.0 27.0 40.0 66-67 34.807977433251466 37.0 33.0 37.0 27.0 40.0 68-69 34.579109752821324 37.0 33.0 37.0 27.0 40.0 70-71 34.284362561756566 37.0 33.0 37.0 27.0 40.0 72-73 34.065215883607095 37.0 33.0 37.0 27.0 38.5 74-75 33.83496169330638 37.0 33.0 37.0 27.0 37.0 76-77 33.5894686601517 37.0 33.0 37.0 27.0 37.0 78-79 33.348827935211105 37.0 33.0 37.0 27.0 37.0 80-81 33.105852797884324 37.0 33.0 37.0 27.0 37.0 82-83 32.90729437184072 37.0 33.0 37.0 27.0 37.0 84-85 32.73015111185631 37.0 33.0 37.0 27.0 37.0 86-87 32.589141514700586 37.0 33.0 37.0 22.0 37.0 88-89 32.447991494599336 33.0 33.0 37.0 22.0 37.0 90-91 32.314295513134404 33.0 33.0 37.0 22.0 37.0 92-93 32.18196794088899 33.0 33.0 37.0 22.0 37.0 94-95 32.03321259881527 33.0 33.0 37.0 22.0 37.0 96-97 31.89334848707137 33.0 33.0 37.0 22.0 37.0 98-99 31.734725813243198 33.0 33.0 37.0 22.0 37.0 100-101 30.43220124875439 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3554942459456498 2306 2 0.36518834515482723 2306 3 0.4188382102687207 2306 4 0.48380193066369515 2306 5 0.4515673465564731 2306 6 0.3475794897100215 2306 7 0.4295950833415745 2306 8 0.481653275036507 2306 9 0.517398010128673 2306 10-11 0.4690155626308581 2306 12-13 0.4496595973990054 2306 14-15 0.8008124687393519 2306 16-17 0.7870672272324342 2306 18-19 0.6119600356900676 2306 20-21 0.6317113401155581 2306 22-23 0.6822938600396427 2306 24-25 0.8871810722470386 2306 26-27 0.897510513748621 2306 28-29 0.8354319709164244 2306 30-31 0.8515748084527388 2306 32-33 0.7512346535317747 2306 34-35 0.791768590082718 2306 36-37 0.6602603458760541 2306 38-39 0.7466421349584849 2306 40-41 0.6992322789019596 2306 42-43 0.6357273317025474 2306 44-45 0.6691529347098069 2306 46-47 0.5193553516574241 2306 48-49 0.7732833725997068 2306 50-51 0.7017115004199113 2306 52-53 0.8165164834236691 2306 54-55 0.8622428635470882 2306 56-57 0.8270782303452364 2306 58-59 0.900116746312051 2306 60-61 0.9501468158268196 2306 62-63 0.8709252344216196 2306 64-65 0.768224145128265 2306 66-67 0.797116450282509 2306 68-69 0.8472212117492006 2306 70-71 0.8167623131661799 2306 72-73 0.9382183315514325 2306 74-75 0.9077782141905502 2306 76-77 0.8005112814980393 2306 78-79 0.879849803431398 2306 80-81 0.7533685453106287 2306 82-83 0.696539558051704 2306 84-85 0.7302301086337337 2306 86-87 0.7453932401510599 2306 88-89 0.9289921610730048 2306 90-91 0.5697022408266719 2306 92-93 0.4778714545188336 2306 94-95 0.5832981055747553 2306 96-97 0.5968919109451427 2306 98-99 0.6614489372285526 2306 100-101 0.6103130345581498 2307 1 0.33095047140187006 2307 2 0.27887125883774644 2307 3 0.27519456662507835 2307 4 0.3550963019580706 2307 5 0.4414803364694606 2307 6 0.44771808984862105 2307 7 0.4992801530266391 2307 8 0.41135220473543654 2307 9 0.4805919733226318 2307 10-11 0.44779241640771517 2307 12-13 0.49185563959504464 2307 14-15 0.6724148403417232 2307 16-17 0.5413311064963153 2307 18-19 0.537250710980743 2307 20-21 0.6096989431031687 2307 22-23 0.48233428508006426 2307 24-25 0.8283626384286009 2307 26-27 0.7552047464428568 2307 28-29 0.6868121972966534 2307 30-31 0.6849273918053242 2307 32-33 0.5676568449539658 2307 34-35 0.5982962716104012 2307 36-37 0.6198738054895045 2307 38-39 0.604827243143589 2307 40-41 0.4860671907368683 2307 42-43 0.5632798842550883 2307 44-45 0.5411018066586735 2307 46-47 0.32049302779510214 2307 48-49 0.4967953461116821 2307 50-51 0.3544604031688081 2307 52-53 0.4922306591378458 2307 54-55 0.5776794539836771 2307 56-57 0.6479664262334381 2307 58-59 0.5840025551978556 2307 60-61 0.6197202354002442 2307 62-63 0.5544164179128046 2307 64-65 0.6762283031324259 2307 66-67 0.7110977392637992 2307 68-69 0.6693991589271491 2307 70-71 0.5306204020242689 2307 72-73 0.4078610511941534 2307 74-75 0.538793845206186 2307 76-77 0.4924724734592303 2307 78-79 0.5316073301889332 2307 80-81 0.4662737582158414 2307 82-83 0.4849625964747446 2307 84-85 0.6603044137080474 2307 86-87 0.40246377222159424 2307 88-89 0.46980220188304855 2307 90-91 0.36842403954846503 2307 92-93 0.675655777303156 2307 94-95 0.4773267496033995 2307 96-97 0.5575352465884826 2307 98-99 0.5725908483704636 2307 100-101 0.6020355262806412 2201 1 0.3291671230030673 2201 2 0.29975267794287674 2201 3 0.3538072647769006 2201 4 0.40170580152004476 2201 5 0.3796955560096933 2201 6 0.42889561879379556 2201 7 0.34256294614140614 2201 8 0.4399943850818886 2201 9 0.39034882571030494 2201 10-11 0.3731610159241825 2201 12-13 0.3660349076773386 2201 14-15 0.5578701468243921 2201 16-17 0.577008046037875 2201 18-19 0.6038875590626205 2201 20-21 0.6568573593206253 2201 22-23 0.5423056823054182 2201 24-25 0.6488225606648115 2201 26-27 0.5951368532976673 2201 28-29 0.6006949951208824 2201 30-31 0.66430018754874 2201 32-33 0.6002902945341262 2201 34-35 0.6181872619766651 2201 36-37 0.6947994272226197 2201 38-39 0.556382254708204 2201 40-41 0.7350582605392688 2201 42-43 0.6417818139332141 2201 44-45 0.6552304576332162 2201 46-47 0.7013467571846022 2201 48-49 0.5833982716488322 2201 50-51 0.5515647326543203 2201 52-53 0.7397640782987196 2201 54-55 0.8109059594137378 2201 56-57 0.8522978580012506 2201 58-59 0.833637211457571 2201 60-61 0.7534705243353628 2201 62-63 0.7432497543697565 2201 64-65 0.7832173132659435 2201 66-67 0.8341822976580033 2201 68-69 0.7528286037344145 2201 70-71 0.6843153836117111 2201 72-73 0.7431646697430878 2201 74-75 0.5988183440208914 2201 76-77 0.5772179246233051 2201 78-79 0.7598476924907374 2201 80-81 0.6796031461731715 2201 82-83 0.6509666946679928 2201 84-85 0.6611468307770565 2201 86-87 0.6153733933165242 2201 88-89 0.6210366095984909 2201 90-91 0.5214385519004736 2201 92-93 0.5878318756290426 2201 94-95 0.5529092932608393 2201 96-97 0.6635713114579538 2201 98-99 0.6173849223292045 2201 100-101 0.434954499021984 1101 1 0.2785215883540957 1101 2 0.28906968434561264 1101 3 0.3410270347376745 1101 4 0.33666488776118086 1101 5 0.3202893455716307 1101 6 0.37817390629792413 1101 7 0.39014193151056986 1101 8 0.3998059912348282 1101 9 0.4373409512442521 1101 10-11 0.4986530723172322 1101 12-13 0.4221938419202189 1101 14-15 0.5344651177014441 1101 16-17 0.5417886370841387 1101 18-19 0.4958716166700512 1101 20-21 0.4662498867876579 1101 22-23 0.45151822006204867 1101 24-25 0.5399150470494192 1101 26-27 0.5831841916861435 1101 28-29 0.533946558274657 1101 30-31 0.5844213483025342 1101 32-33 0.4760098233395098 1101 34-35 0.5326727141301717 1101 36-37 0.5308615731548016 1101 38-39 0.508147268613456 1101 40-41 0.45387387597026674 1101 42-43 0.28685827884326187 1101 44-45 0.23181008124316804 1101 46-47 0.31532545910962284 1101 48-49 0.2825259347738651 1101 50-51 0.2668126111236191 1101 52-53 0.4016491516182228 1101 54-55 0.5398365855219183 1101 56-57 0.6724072234755312 1101 58-59 0.6247867083598635 1101 60-61 0.5830321978978716 1101 62-63 0.5156340338535443 1101 64-65 0.48335103777959176 1101 66-67 0.49108654612556535 1101 68-69 0.40474700095251137 1101 70-71 0.36228571088827266 1101 72-73 0.4125174060133716 1101 74-75 0.3637230088975656 1101 76-77 0.3869226472253615 1101 78-79 0.556076308240975 1101 80-81 0.33564464143037753 1101 82-83 0.3525522661816751 1101 84-85 0.44206593325458243 1101 86-87 0.41722085131092257 1101 88-89 0.2860133641854148 1101 90-91 0.24821006102830268 1101 92-93 0.1315503643052338 1101 94-95 0.2720696078641751 1101 96-97 0.308324076367537 1101 98-99 0.1176909740894807 1101 100-101 0.24668284926672612 2304 1 0.3619077346352526 2304 2 0.37890646195130273 2304 3 0.42294773023645504 2304 4 0.5211289390727956 2304 5 0.4464251133358843 2304 6 0.3702254853037772 2304 7 0.464581275044182 2304 8 0.4499145543985321 2304 9 0.49299009906031444 2304 10-11 0.4740777969375003 2304 12-13 0.45007365238394925 2304 14-15 0.5578373430683286 2304 16-17 0.6224987947069138 2304 18-19 0.5827948558719029 2304 20-21 0.5243484340119835 2304 22-23 0.5441696745740359 2304 24-25 0.6202751199399614 2304 26-27 0.6200805831004104 2304 28-29 0.5884869597456657 2304 30-31 0.5950919873710419 2304 32-33 0.5619606170095963 2304 34-35 0.5533868565983795 2304 36-37 0.54159916891264 2304 38-39 0.5342445335683479 2304 40-41 0.5897006749673679 2304 42-43 0.5507132131063344 2304 44-45 0.6224345386443915 2304 46-47 0.5992708728042899 2304 48-49 0.6743140495165747 2304 50-51 0.5812326511630701 2304 52-53 0.590265494346184 2304 54-55 0.8851497040845331 2304 56-57 0.9162876859259654 2304 58-59 0.9063241771929853 2304 60-61 0.7123473774676867 2304 62-63 0.8062909603657005 2304 64-65 0.7719196101110484 2304 66-67 0.8856325819422963 2304 68-69 0.8407577843436087 2304 70-71 0.9075843529154568 2304 72-73 0.889346267754 2304 74-75 0.88094173003131 2304 76-77 0.7930767157612308 2304 78-79 0.739341650329969 2304 80-81 0.7608540194472297 2304 82-83 0.9323909016455829 2304 84-85 0.8747973581450168 2304 86-87 0.7166135096455832 2304 88-89 0.8767688700437404 2304 90-91 0.788446907565735 2304 92-93 0.8102073064442337 2304 94-95 0.9161958892654525 2304 96-97 0.9279312956225887 2304 98-99 0.8833853182134384 2304 100-101 0.8799501013034288 2202 1 0.2607466558953462 2202 2 0.2200865869006705 2202 3 0.19506119734765903 2202 4 0.35246715924542116 2202 5 0.29506753535937946 2202 6 0.39522509581074416 2202 7 0.2801547645901863 2202 8 0.31492425277512837 2202 9 0.238451920952933 2202 10-11 0.24467311756816912 2202 12-13 0.3057444762396244 2202 14-15 0.4554611434297797 2202 16-17 0.4470517540749981 2202 18-19 0.2833119078770139 2202 20-21 0.313509329690163 2202 22-23 0.33280417041424926 2202 24-25 0.4282798834397923 2202 26-27 0.37975685596364883 2202 28-29 0.4898199022876568 2202 30-31 0.4829640505935444 2202 32-33 0.2996414391515003 2202 34-35 0.4086947703178794 2202 36-37 0.4244928295239845 2202 38-39 0.38214945052843063 2202 40-41 0.34933829064679855 2202 42-43 0.3951317779023995 2202 44-45 0.4393496369524357 2202 46-47 0.3618285177735956 2202 48-49 0.3023627088815459 2202 50-51 0.3796163524374947 2202 52-53 0.4979091978017678 2202 54-55 0.5823062101761991 2202 56-57 0.5509534424772369 2202 58-59 0.6787643315880274 2202 60-61 0.6157729687181117 2202 62-63 0.572758141682499 2202 64-65 0.4620574516964382 2202 66-67 0.5953413774740355 2202 68-69 0.5573105567550414 2202 70-71 0.6591048363542171 2202 72-73 0.5485102599018106 2202 74-75 0.5458886226558732 2202 76-77 0.6105215245980489 2202 78-79 0.5819184973059421 2202 80-81 0.5296629302689055 2202 82-83 0.48371615170011495 2202 84-85 0.5882923181051325 2202 86-87 0.5067512538577148 2202 88-89 0.6118061055779691 2202 90-91 0.4399529797621682 2202 92-93 0.5806560803201606 2202 94-95 0.550052084541683 2202 96-97 0.4785772698225621 2202 98-99 0.5414008901450096 2202 100-101 0.5202655517131731 1102 1 0.0890759275352977 1102 2 0.08737817212553267 1102 3 0.15526547418602732 1102 4 0.08150757378766826 1102 5 0.05438766610986079 1102 6 0.14900662938417497 1102 7 0.1303123804732138 1102 8 0.20927004289232087 1102 9 0.12120150715925604 1102 10-11 0.19928015913051667 1102 12-13 0.17768968080757475 1102 14-15 0.14253456165666734 1102 16-17 0.1440300205498133 1102 18-19 0.13414282221548746 1102 20-21 0.05897132305283037 1102 22-23 0.12715619862708394 1102 24-25 0.16279069198016316 1102 26-27 0.25244980026161556 1102 28-29 0.10399451906064172 1102 30-31 0.1395286065658965 1102 32-33 -0.015347872911313232 1102 34-35 0.12495041579443011 1102 36-37 0.1554649743476091 1102 38-39 0.19034921930302318 1102 40-41 0.28943011541453956 1102 42-43 0.1940696381524205 1102 44-45 0.04837740895419529 1102 46-47 0.059930473208645196 1102 48-49 0.030196675728149103 1102 50-51 0.04768270914011907 1102 52-53 0.19029206715941172 1102 54-55 0.2821211466723881 1102 56-57 0.33112627931360805 1102 58-59 0.384189395424535 1102 60-61 0.4227230390006511 1102 62-63 0.38035031994232327 1102 64-65 0.4327966244417851 1102 66-67 0.3938101329323658 1102 68-69 0.23535495536103213 1102 70-71 0.32069812915378293 1102 72-73 0.31005447968239963 1102 74-75 0.2671879763891596 1102 76-77 0.3933967044631359 1102 78-79 0.30894941330790004 1102 80-81 0.3357438258930827 1102 82-83 0.3338808374248572 1102 84-85 0.4483847250951243 1102 86-87 0.3284856107932299 1102 88-89 0.3893751612966412 1102 90-91 0.3801084331053701 1102 92-93 0.4455651288858107 1102 94-95 0.45936556132348727 1102 96-97 0.4615851131881463 1102 98-99 0.43795621373582705 1102 100-101 0.2490955344960284 2305 1 0.03588053639361988 2305 2 0.13321210701051456 2305 3 0.11019456276617134 2305 4 0.08851823584260643 2305 5 0.04553118762902386 2305 6 0.17489167824040663 2305 7 0.09129159392011843 2305 8 0.07963806962885656 2305 9 0.11515598992211551 2305 10-11 -0.01373869836164232 2305 12-13 0.07893481591862894 2305 14-15 0.05285580559378644 2305 16-17 0.04090604766724226 2305 18-19 0.025037070972196318 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-0.7047330731097006 1213 56-57 -0.8386687359336449 1213 58-59 -1.236314078948567 1213 60-61 -0.8924941066013261 1213 62-63 -0.8194472165891327 1213 64-65 -0.6873132253027165 1213 66-67 -0.7721412280177233 1213 68-69 -0.6116687338428761 1213 70-71 -0.7019247454145017 1213 72-73 -0.7235752132208333 1213 74-75 -0.6654365717901882 1213 76-77 -0.9031203370271967 1213 78-79 -0.8792753200554202 1213 80-81 -0.7806069195158969 1213 82-83 -0.6228710964653317 1213 84-85 -0.5774898453628055 1213 86-87 -0.8291540976941505 1213 88-89 -0.5896856820729255 1213 90-91 -0.5304179284424322 1213 92-93 -0.4500325132787495 1213 94-95 -0.531352961203968 1213 96-97 -0.6444179340881071 1213 98-99 -0.6755189635691359 1213 100-101 -1.206716351620173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1250.0 3 109.0 4 85.0 5 136.0 6 338.0 7 1432.0 8 4504.0 9 9019.0 10 12467.0 11 10379.0 12 8121.0 13 6550.0 14 5725.0 15 5657.0 16 5722.0 17 6085.0 18 6757.0 19 7366.0 20 8202.0 21 9290.0 22 10497.0 23 11640.0 24 13406.0 25 15505.0 26 18163.0 27 21754.0 28 26636.0 29 33012.0 30 41161.0 31 53658.0 32 74037.0 33 105597.0 34 160628.0 35 266834.0 36 516619.0 37 885083.0 38 259899.0 39 892.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.67365541100954 25.054018680673163 23.978683375529165 14.293642532788128 2 32.80134346017328 22.307812678905385 32.44559367963055 12.44525018129079 3 38.99579840269097 18.50877634773903 26.082029586692855 16.41339566287714 4 31.730076610810976 17.737615602966763 33.75568476635229 16.776623019869973 5 31.01403188252505 16.558338732559356 35.54415422173686 16.88347516317873 6 25.295018996242728 17.144552724830216 40.640777328924095 16.919650950002957 7 33.82334480428476 17.26663723570802 30.172163476892568 18.737854483114656 8 30.77654730683035 26.530672199334767 24.85759458657219 17.835185907262698 9 29.768360301629848 26.73725958768912 24.805301030891652 18.68907907978938 10-11 24.521450521214785 28.17249066207705 30.1769589802744 17.129099836433767 12-13 25.246410864361085 25.822488787885955 29.757412357783387 19.173687989969572 14-15 23.472581459333384 26.803219798932354 30.828025696428224 18.896173045306035 16-17 22.619780662499057 27.89230060209846 31.64067060231973 17.847248133082758 18-19 22.156618620872116 28.007093658302285 31.544251719956534 18.292036000869064 20-21 22.10333390617928 28.361424719364297 31.086995208121415 18.448246166335007 22-23 22.32961740244489 27.76116658279465 31.059339800698726 18.84987621406173 24-25 22.16530762961234 27.9453540830422 30.96737163126538 18.92196665608008 26-27 21.811839924658916 28.358071188762434 30.960400066045757 18.869688820532893 28-29 21.346346848943107 28.58570307432734 30.479621384659023 19.58832869207053 30-31 21.290229689161627 28.869534730644236 30.31926340897343 19.520972171220713 32-33 20.70840225703595 29.185009971052523 31.39835629983497 18.708231472076562 34-35 21.511830628932103 28.765271067113314 31.230078568987118 18.49281973496747 36-37 20.950424575758582 29.103834307127443 31.546611246185165 18.399129870928814 38-39 21.238617934262198 29.058002267479665 30.85067941265665 18.852700385601484 40-41 21.354461440395724 28.98186961238811 29.983984053605077 19.679684893611086 42-43 21.417430801520457 28.761824318511266 30.699905592813277 19.120839287155004 44-45 20.774534114136138 28.850347679154936 31.328212523481312 19.046905683227614 46-47 21.098359085959675 28.782404052887383 30.830235264638556 19.28900159651439 48-49 20.869487934450103 29.741429976233004 29.9064897186473 19.482592370669593 50-51 20.991945559851537 29.20380097862757 29.744681835183766 20.059571626337128 52-53 21.380638159480426 28.816243350554217 29.747065413286794 20.056053076678566 54-55 22.021593041978743 28.994557978605695 29.36581857151429 19.61803040790127 56-57 22.041233436799484 29.179958286086805 29.11790152986054 19.660906747253172 58-59 21.592600053283775 29.215218671796787 29.768563413617024 19.423617861302407 60-61 20.808388039099242 29.473777537273676 29.680390686682518 20.037443736944564 62-63 21.174869626543316 29.22167242208499 29.446602819196432 20.15685513217527 64-65 20.742313894599782 29.216955589310277 29.691886974203243 20.348843541886698 66-67 21.185862715306918 28.397324833193803 29.772992234724317 20.643820216774962 68-69 21.245361470877107 28.704116300488792 30.20273859523746 19.847783633396645 70-71 20.55700751544457 30.310293624050495 29.43656421100249 19.696134649502444 72-73 20.343917698749582 30.587564271587404 28.993625083712082 20.074892945950936 74-75 20.92045525314277 29.80420706842819 29.060938190701535 20.214399487727505 76-77 20.85042848841452 29.424150998469123 29.29379546636296 20.431625046753393 78-79 20.716173794645297 29.621737740153097 29.461912651873952 20.200175813327657 80-81 19.99384864612904 30.04921083096767 29.481815298913773 20.475125223989515 82-83 19.489281270843566 29.754836662888945 29.598484834006378 21.15739723226111 84-85 19.632941779080834 29.370678154636536 30.061447132530855 20.934932933751774 86-87 19.944196855148796 29.363920591360447 30.664764518372994 20.02711803511776 88-89 20.165220010488703 30.032035649359283 29.895575479674697 19.907168860477313 90-91 20.42561460542831 29.63223319647941 29.462329759794525 20.47982243829775 92-93 19.84271328655297 30.208811341913766 29.68128091334425 20.267194458189014 94-95 19.492311668396322 30.622575399592865 29.796847878595823 20.08826505341499 96-97 19.846373749253367 31.143345215143796 29.2968159791472 19.713465056455632 98-99 20.46899634468902 30.263938213551782 28.800801218487944 20.466264223271256 100-101 20.456485974301838 30.095312042958533 28.173194440809073 21.275007541930556 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1180.0 1 705.5 2 204.0 3 209.0 4 239.5 5 238.5 6 303.0 7 448.0 8 593.0 9 735.5 10 874.5 11 986.0 12 1046.5 13 999.0 14 899.5 15 812.5 16 731.0 17 661.5 18 688.5 19 782.0 20 898.0 21 1061.5 22 1272.5 23 1678.0 24 2311.0 25 3282.5 26 5082.0 27 7875.0 28 10782.5 29 13632.0 30 17064.0 31 20457.5 32 25927.0 33 36782.0 34 53748.5 35 77940.0 36 112283.5 37 150413.5 38 183605.5 39 202579.0 40 201449.0 41 184555.0 42 165804.0 43 157510.0 44 145949.0 45 131705.5 46 123634.0 47 109641.0 48 90988.0 49 76395.5 50 63320.5 51 51712.5 52 42110.0 53 33848.0 54 27785.5 55 21862.0 56 15633.0 57 10481.5 58 7178.5 59 5269.0 60 4000.5 61 3008.5 62 2147.0 63 1531.0 64 1156.5 65 888.5 66 698.0 67 543.0 68 410.0 69 310.0 70 235.5 71 186.0 72 143.5 73 100.0 74 80.5 75 70.0 76 65.0 77 65.5 78 45.5 79 34.0 80 29.5 81 27.0 82 27.0 83 24.0 84 23.5 85 21.0 86 15.5 87 15.0 88 14.5 89 9.5 90 8.5 91 5.5 92 3.5 93 4.5 94 3.0 95 3.0 96 3.0 97 2.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1634012457058587 2 0.1568087980596102 3 0.12601863795458831 4 0.12098856229386694 5 0.13219191262911004 6 0.1509022698216419 7 0.12925770182702256 8 0.12106477556145362 9 0.11390072840830497 10-11 0.10086825965098134 12-13 0.12914338192564254 14-15 0.12598053132079498 16-17 0.1161871264359056 18-19 0.11433895469692842 20-21 0.10671762793825962 22-23 0.12034074951938008 24-25 0.41326644348881475 26-27 0.2990227553763697 28-29 0.38729677255865086 30-31 0.222104515064505 32-33 0.2624975468854496 34-35 0.274958416135873 36-37 0.2717765122141288 38-39 0.29164912173735763 40-41 0.28381820849282546 42-43 0.34097815918284136 44-45 0.2860474465697361 46-47 0.3126077703236968 48-49 0.31218859735197 50-51 0.31264587695749013 52-53 0.3163422204354445 54-55 0.29433563941978835 56-57 0.30719662832504196 58-59 0.306148695895725 60-61 0.2853805804783526 62-63 0.28219867655660835 64-65 0.29062024262493735 66-67 0.29077266916011074 68-69 0.28751455197077985 70-71 0.2618497341109627 72-73 0.25196106264158996 74-75 0.2613352945547526 76-77 0.2575246311754182 78-79 0.2540188208664306 80-81 0.26312630634303974 82-83 0.3048530703467513 84-85 0.3103785322467862 86-87 0.3000897411225833 88-89 0.30740621481090535 90-91 0.2833037689366153 92-93 0.2675085692292743 94-95 0.25319952823987363 96-97 0.25258982209918013 98-99 0.2744058699458695 100-101 0.27358657731931263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2624215.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.6200225958695 #Duplication Level Percentage of deduplicated Percentage of total 1 87.89454402091542 42.73434716350554 2 5.573543170352933 5.419715897632274 3 1.8032472035600349 2.6302175934908223 4 0.9552798547778022 1.8578291249870273 5 0.6588257719322774 1.6016061959044248 6 0.4299016503336349 1.254109677193375 7 0.3458417331402778 1.1770383015912482 8 0.24192151420832295 0.9409783589788501 9 0.21021938179120425 0.919878398350026 >10 1.5651850777060397 15.185628669374662 >50 0.17069883373642744 5.742896231193178 >100 0.13394773978929025 13.787751195978782 >500 0.014287128888040073 4.450116857519397 >1k 0.0023985549113062386 1.8449923819314564 >5k 1.5836395704757467E-4 0.45289395236916896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6045 0.23035460128076396 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5719 0.21793183866413382 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2874 0.1095184655220704 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2694 0.10265927143926849 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2683 0.10224009846754173 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.953862393134708E-4 0.0 0.0 2 6.478127744868465E-4 0.0 0.0 3 6.478127744868465E-4 0.0 0.0 4 6.478127744868465E-4 0.0 0.0 5 7.621326758668783E-4 0.0 0.0 6 8.7645257724691E-4 0.0 0.0 7 9.14559211040254E-4 0.0 0.0 8 0.0011431990138003174 0.0 0.0 9 0.0012575189151803493 0.0 0.0 10-11 0.0020577582248405713 0.0 0.0 12-13 0.0030294773865708413 0.0 0.0 14-15 0.004096463132784471 0.0 0.0 16-17 0.005487355266241524 0.0 0.0 18-19 0.006992567301078608 0.0 0.0 20-21 0.008859792356952459 0.0 0.0 22-23 0.010974710532483048 0.0 0.0 24-25 0.013223001926290338 0.0 0.0 26-27 0.016195319362171164 0.0 0.0 28-29 0.019072370213568626 0.0 0.0 30-31 0.028999148316734717 0.0 0.0 32-33 0.033343304569175926 0.0 0.0 34-35 0.038030420525757225 0.0 0.0 36-37 0.04206972370785168 0.0 0.0 38-39 0.04643293327718956 0.0 0.0 40-41 0.051520168888600976 0.0 0.0 42-43 0.05756007034484598 0.0 0.0 44-45 0.0630474256110875 0.0 0.0 46-47 0.06994472632768275 0.0 0.0 48-49 0.07663244055841462 0.0 0.0 50-51 0.08352974127500987 0.0 0.0 52-53 0.09136065451954203 0.0 0.0 54-55 0.09930588766545424 0.0 0.0 56-57 0.10866106626172017 0.0 0.0 58-59 0.11864500431557627 0.0 0.0 60-61 0.12961971484805934 0.0 0.0 62-63 0.14154709122537595 0.0 0.0 64-65 0.15509399953890973 0.0 0.0 66-67 0.1706796127603874 0.0 0.0 68-69 0.18710357192531862 0.0 0.0 70-71 0.20569960921647046 0.0 0.0 72-73 0.2279538833517833 0.0 3.8106633793343915E-5 74-75 0.25009383758571613 0.0 3.8106633793343915E-5 76-77 0.27646362817071013 0.0 3.8106633793343915E-5 78-79 0.306377335698485 0.0 3.8106633793343915E-5 80-81 0.3393967338804176 0.0 3.8106633793343915E-5 82-83 0.37902763302549525 0.0 5.715995069001587E-5 84-85 0.4230407950568075 0.0 7.621326758668783E-5 86-87 0.47088367378435075 0.0 7.621326758668783E-5 88-89 0.5264812524888396 0.0 9.526658448335979E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 680 0.0 63.467293 2 GAGCGGC 425 0.0 59.13074 9 CACGCGG 25 0.005051504 56.91568 1 TCGCCGC 35 2.8101262E-4 54.20127 2 AGAGCGG 455 0.0 54.18989 8 CGGGAGA 665 0.0 52.0538 4 GAGAGCG 720 0.0 46.757603 7 GATCCGG 180 0.0 44.794746 1 AAGCGGC 205 0.0 43.946682 9 CGGAAAG 280 0.0 42.33828 5 GATCGGG 1180 0.0 41.802475 1 ATTCGGA 125 3.6379788E-12 41.734978 2 GGCGCCG 405 0.0 41.054462 56-57 CCGGATC 255 0.0 40.981934 60-61 TTGTGTT 32925 0.0 39.946133 1 TCGGAAA 250 0.0 39.831852 4 ACCGGAA 60 8.3776176E-5 39.515728 3 TCGGGAA 265 0.0 39.36661 3 TCGGGAG 930 0.0 38.241024 3 GGTCGCA 50 0.0016298225 37.9351 3 >>END_MODULE