FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624215
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60450.23035460128076396No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC57190.21793183866413382No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28740.1095184655220704No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA26940.10265927143926849No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA26830.10224009846754173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA6800.063.4672932
GAGCGGC4250.059.130749
CACGCGG250.00505150456.915681
TCGCCGC352.8101262E-454.201272
AGAGCGG4550.054.189898
CGGGAGA6650.052.05384
GAGAGCG7200.046.7576037
GATCCGG1800.044.7947461
AAGCGGC2050.043.9466829
CGGAAAG2800.042.338285
GATCGGG11800.041.8024751
ATTCGGA1253.6379788E-1241.7349782
GGCGCCG4050.041.05446256-57
CCGGATC2550.040.98193460-61
TTGTGTT329250.039.9461331
TCGGAAA2500.039.8318524
ACCGGAA608.3776176E-539.5157283
TCGGGAA2650.039.366613
TCGGGAG9300.038.2410243
GGTCGCA500.001629822537.93513
Produced by FastQC (version 0.11.2)