##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34344-1.34200000035854.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4820689 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.640357633525 33.0 33.0 33.0 27.0 33.0 2 31.524328161389377 33.0 33.0 33.0 27.0 33.0 3 31.426544213908013 33.0 33.0 33.0 27.0 33.0 4 35.06273750495002 37.0 37.0 37.0 33.0 37.0 5 35.07186711277164 37.0 37.0 37.0 33.0 37.0 6 35.25063823864182 37.0 37.0 37.0 33.0 37.0 7 35.00279959980824 37.0 37.0 37.0 33.0 37.0 8 35.10295437021554 37.0 37.0 37.0 33.0 37.0 9 34.886956200659284 37.0 37.0 37.0 33.0 37.0 10-11 34.8438713843602 37.0 37.0 37.0 33.0 37.0 12-13 34.80354053123941 37.0 37.0 37.0 33.0 37.0 14-15 36.39156902675116 40.0 37.0 40.0 33.0 40.0 16-17 36.35595015567277 40.0 37.0 40.0 33.0 40.0 18-19 36.403428638520346 40.0 37.0 40.0 33.0 40.0 20-21 36.38343543837821 40.0 37.0 40.0 33.0 40.0 22-23 36.56091390670504 40.0 37.0 40.0 33.0 40.0 24-25 36.18757225367577 40.0 37.0 40.0 27.0 40.0 26-27 36.099886344047505 40.0 37.0 40.0 27.0 40.0 28-29 35.918490697076706 40.0 37.0 40.0 27.0 40.0 30-31 35.94924096949627 40.0 37.0 40.0 27.0 40.0 32-33 35.842563791192504 40.0 37.0 40.0 27.0 40.0 34-35 35.803032823731215 38.5 37.0 40.0 27.0 40.0 36-37 35.71534255787917 37.0 37.0 40.0 27.0 40.0 38-39 35.61234109895909 37.0 37.0 40.0 27.0 40.0 40-41 35.534761524752994 37.0 37.0 40.0 27.0 40.0 42-43 35.3827451843502 37.0 37.0 40.0 27.0 40.0 44-45 35.25476503462472 37.0 37.0 40.0 27.0 40.0 46-47 35.12788047517689 37.0 37.0 40.0 27.0 40.0 48-49 35.006433001589606 37.0 37.0 40.0 24.5 40.0 50-51 33.92279350524375 37.0 33.0 38.5 22.0 38.5 52-53 34.38434018456698 37.0 35.0 38.5 24.5 40.0 54-55 35.0579456588052 37.0 37.0 40.0 27.0 40.0 56-57 35.17674247394926 37.0 37.0 40.0 27.0 40.0 58-59 34.88294401484933 37.0 35.0 40.0 27.0 40.0 60-61 34.70095913675411 37.0 33.0 40.0 27.0 40.0 62-63 34.43830944082889 37.0 33.0 40.0 27.0 40.0 64-65 34.18787988604949 37.0 33.0 40.0 24.5 40.0 66-67 33.95500269774715 37.0 33.0 37.0 22.0 40.0 68-69 33.80746175494831 37.0 33.0 37.0 22.0 40.0 70-71 33.46602954473936 37.0 33.0 37.0 22.0 40.0 72-73 33.36510807894888 37.0 33.0 37.0 22.0 38.5 74-75 33.185064624579596 37.0 33.0 37.0 27.0 37.0 76-77 32.90837938311307 37.0 33.0 37.0 22.0 37.0 78-79 32.602396877292854 37.0 33.0 37.0 22.0 37.0 80-81 32.27813026312214 37.0 33.0 37.0 22.0 37.0 82-83 32.04543178371391 37.0 33.0 37.0 22.0 37.0 84-85 31.85391942935958 33.0 33.0 37.0 22.0 37.0 86-87 31.71223501453838 33.0 33.0 37.0 15.0 37.0 88-89 31.56752779530063 33.0 33.0 37.0 15.0 37.0 90-91 31.42305332287563 33.0 33.0 37.0 15.0 37.0 92-93 31.262717839711293 33.0 33.0 37.0 15.0 37.0 94-95 31.10628179913701 33.0 33.0 37.0 15.0 37.0 96-97 30.97359391987328 33.0 33.0 37.0 15.0 37.0 98-99 30.8063128527893 33.0 27.0 37.0 15.0 37.0 100-101 29.54854129772736 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.29850980597496957 2306 2 0.19191621313572682 2306 3 0.2394166861793856 2306 4 0.35803145017288784 2306 5 0.3112209872265126 2306 6 0.24124254495460917 2306 7 0.414988906862618 2306 8 0.23323224808177656 2306 9 0.34458819814769015 2306 10-11 0.367865125182405 2306 12-13 0.34069642069558626 2306 14-15 0.6116326571561572 2306 16-17 0.5887796305766386 2306 18-19 0.5112128507440659 2306 20-21 0.4253781893820374 2306 22-23 0.49912749462680495 2306 24-25 0.7157584703273869 2306 26-27 0.5660050363018172 2306 28-29 0.5622022646966656 2306 30-31 0.46534322845717924 2306 32-33 0.5278201413143719 2306 34-35 0.5009248257489176 2306 36-37 0.5191082428113205 2306 38-39 0.5448533590659466 2306 40-41 0.45829312821410184 2306 42-43 0.4918657429519939 2306 44-45 0.54898167439476 2306 46-47 0.4221610318671978 2306 48-49 0.5138945364172258 2306 50-51 0.4581992710401934 2306 52-53 0.41205736545585125 2306 54-55 0.7077485147832547 2306 56-57 0.6169078435442188 2306 58-59 0.770799658345453 2306 60-61 0.6387629131889838 2306 62-63 0.5700543351621405 2306 64-65 0.7154183007059984 2306 66-67 0.5162821068925822 2306 68-69 0.5748617256465423 2306 70-71 0.6434377673482317 2306 72-73 0.48847371587954314 2306 74-75 0.5986124945999762 2306 76-77 0.6406925774107393 2306 78-79 0.61055948208773 2306 80-81 0.6277856493756815 2306 82-83 0.5050224260398366 2306 84-85 0.6649324140509769 2306 86-87 0.47347971979738546 2306 88-89 0.5922098287057871 2306 90-91 0.6219332256231915 2306 92-93 0.5492768169948441 2306 94-95 0.5422124061526077 2306 96-97 0.6151044180654175 2306 98-99 0.5544054237864238 2306 100-101 0.5329169010532766 2307 1 0.22318027513225402 2307 2 0.17725886368753763 2307 3 0.21057268624304726 2307 4 0.27721672645287043 2307 5 0.232087738444001 2307 6 0.29876438084921375 2307 7 0.4355237577081752 2307 8 0.31481543566035697 2307 9 0.34787394531666394 2307 10-11 0.2944815182387259 2307 12-13 0.40177314426975386 2307 14-15 0.6398851062041473 2307 16-17 0.4872613384050126 2307 18-19 0.5743083656944279 2307 20-21 0.4453532612221238 2307 22-23 0.39479801438155704 2307 24-25 0.5812017552003823 2307 26-27 0.5421891298363448 2307 28-29 0.5827382438894659 2307 30-31 0.6403412152481494 2307 32-33 0.5449468490085323 2307 34-35 0.4943583792802073 2307 36-37 0.5292409198187116 2307 38-39 0.4404077184414561 2307 40-41 0.47176446593746135 2307 42-43 0.48667617705211796 2307 44-45 0.42406128226202355 2307 46-47 0.3370349527218437 2307 48-49 0.4812860731477784 2307 50-51 0.37232040612426687 2307 52-53 0.3939245877286126 2307 54-55 0.536835441942145 2307 56-57 0.5737787893628834 2307 58-59 0.47532963995695354 2307 60-61 0.4235801458781836 2307 62-63 0.5010972938358904 2307 64-65 0.5787625033885178 2307 66-67 0.5487005921184718 2307 68-69 0.5796219851141515 2307 70-71 0.485861937068762 2307 72-73 0.4576989356518908 2307 74-75 0.516789741085887 2307 76-77 0.46614005023359795 2307 78-79 0.5042080035846439 2307 80-81 0.5208656026359719 2307 82-83 0.5632039887508853 2307 84-85 0.5489634975232569 2307 86-87 0.39443578860708683 2307 88-89 0.4224776603335805 2307 90-91 0.5120504953459282 2307 92-93 0.5434778841975465 2307 94-95 0.5413292665822311 2307 96-97 0.5258276838966687 2307 98-99 0.41228165573376074 2307 100-101 0.4858632176171511 2201 1 0.36322073756250717 2201 2 0.3250896013464306 2201 3 0.41385650816499364 2201 4 0.4752083902903763 2201 5 0.46659162924573394 2201 6 0.4814777050511836 2201 7 0.5664029397184365 2201 8 0.48892854228787996 2201 9 0.5454177127712896 2201 10-11 0.4860399288265924 2201 12-13 0.46254320952908046 2201 14-15 0.6435084925522787 2201 16-17 0.5825270129356568 2201 18-19 0.5389663926870085 2201 20-21 0.6371634780291231 2201 22-23 0.5122754255317901 2201 24-25 0.7032253363162368 2201 26-27 0.6497440968684813 2201 28-29 0.597486413823006 2201 30-31 0.7223414823383436 2201 32-33 0.5147670660886874 2201 34-35 0.5904435350922128 2201 36-37 0.6760529425196395 2201 38-39 0.582763974270506 2201 40-41 0.4329987738464993 2201 42-43 0.55463361345452 2201 44-45 0.6413604020350689 2201 46-47 0.4441315088625899 2201 48-49 0.5961448296955041 2201 50-51 0.5560305490912256 2201 52-53 0.6742608959090788 2201 54-55 0.741775569945986 2201 56-57 0.7808601916037503 2201 58-59 0.9462385964537745 2201 60-61 0.7492895933700225 2201 62-63 0.6971295735937915 2201 64-65 0.8090094274069912 2201 66-67 0.7989991174423992 2201 68-69 0.7774674214055537 2201 70-71 0.7154556607960387 2201 72-73 0.6479409731338421 2201 74-75 0.6451674456451073 2201 76-77 0.6847058972889144 2201 78-79 0.7465119253445707 2201 80-81 0.7065581098454601 2201 82-83 0.5306762185247678 2201 84-85 0.6118049209579048 2201 86-87 0.6070259261281912 2201 88-89 0.7334601837028849 2201 90-91 0.5724210092820918 2201 92-93 0.5004155520231954 2201 94-95 0.48535923705055595 2201 96-97 0.7048594125642431 2201 98-99 0.5041610081440986 2201 100-101 0.5579275993618857 1101 1 0.3332627822224694 1101 2 0.23036198354419568 1101 3 0.2231889697299998 1101 4 0.2304231086456383 1101 5 0.3150764777305852 1101 6 0.24567345336440383 1101 7 0.3159420830244386 1101 8 0.3298853080273858 1101 9 0.2914198377065915 1101 10-11 0.3200407097857152 1101 12-13 0.3082061620994381 1101 14-15 0.5087430981225154 1101 16-17 0.4507674030519908 1101 18-19 0.45484528953684134 1101 20-21 0.4555450764526796 1101 22-23 0.4782038133960427 1101 24-25 0.3606975714031009 1101 26-27 0.4417660545332325 1101 28-29 0.3178303990755893 1101 30-31 0.3500260293366466 1101 32-33 0.4117440277944553 1101 34-35 0.4872479593164698 1101 36-37 0.4562855956920586 1101 38-39 0.3726678073268701 1101 40-41 0.3157867979290998 1101 42-43 0.28520952540591793 1101 44-45 0.22360629518896502 1101 46-47 0.28514048989254803 1101 48-49 0.3250453118923602 1101 50-51 0.1895200742499199 1101 52-53 0.43525561989451234 1101 54-55 0.5990121551567995 1101 56-57 0.5534762230600521 1101 58-59 0.4889370826643997 1101 60-61 0.5924439417686003 1101 62-63 0.4973243593294754 1101 64-65 0.5007031098108996 1101 66-67 0.451183986813362 1101 68-69 0.3856711246047624 1101 70-71 0.4531124201789041 1101 72-73 0.46463579065274985 1101 74-75 0.4839152775754414 1101 76-77 0.5952928879628061 1101 78-79 0.5471721082342995 1101 80-81 0.475839173402818 1101 82-83 0.468819261177849 1101 84-85 0.5466084552520023 1101 86-87 0.5312002434700211 1101 88-89 0.5945947538575318 1101 90-91 0.42634305866414124 1101 92-93 0.437434830816521 1101 94-95 0.4184873415901045 1101 96-97 0.382527613906408 1101 98-99 0.3782199978009295 1101 100-101 0.5245461371521358 2304 1 0.28469542152463134 2304 2 0.2678580768972978 2304 3 0.23856760810210176 2304 4 0.29917651117202126 2304 5 0.25300125955089925 2304 6 0.31639088185094266 2304 7 0.3102083839621983 2304 8 0.31204793955576093 2304 9 0.2289305746696897 2304 10-11 0.3222580132411892 2304 12-13 0.31234017997713437 2304 14-15 0.494625886405494 2304 16-17 0.4490276705284586 2304 18-19 0.44143917482831085 2304 20-21 0.28111028397026416 2304 22-23 0.3848910833953667 2304 24-25 0.4918129092157528 2304 26-27 0.5102087167158089 2304 28-29 0.4529282659774836 2304 30-31 0.36549322755839597 2304 32-33 0.31425561820257286 2304 34-35 0.43733855861493254 2304 36-37 0.30381924981293906 2304 38-39 0.32232846876605237 2304 40-41 0.4670311281235726 2304 42-43 0.40074237183156214 2304 44-45 0.4480156680547722 2304 46-47 0.29529841488750463 2304 48-49 0.38905943039021196 2304 50-51 0.45630414089983873 2304 52-53 0.48423950586029463 2304 54-55 0.5804833951618917 2304 56-57 0.5866772096388289 2304 58-59 0.6977649746809078 2304 60-61 0.5464078861508099 2304 62-63 0.5389444111167805 2304 64-65 0.5470360797793674 2304 66-67 0.6554858641903039 2304 68-69 0.6534991226358642 2304 70-71 0.5938323217268078 2304 72-73 0.7157980468123029 2304 74-75 0.7381684011015466 2304 76-77 0.6470812916568178 2304 78-79 0.6379940924636642 2304 80-81 0.4294464898427819 2304 82-83 0.5113868298627224 2304 84-85 0.45373103733384923 2304 86-87 0.5812913572240426 2304 88-89 0.6052650674016569 2304 90-91 0.6457816425002783 2304 92-93 0.5726996661071446 2304 94-95 0.6607290761725615 2304 96-97 0.5159570371263946 2304 98-99 0.5617748930568602 2304 100-101 0.5715003581405611 2202 1 0.12620214582999267 2202 2 0.030307689761624346 2202 3 0.0387177744750673 2202 4 0.09231853207762697 2202 5 0.040488708227982784 2202 6 0.141553221556471 2202 7 0.10313546116425698 2202 8 0.02324279607552171 2202 9 0.03888895731884645 2202 10-11 0.07827443461322758 2202 12-13 0.08268280350969093 2202 14-15 0.21748687373101205 2202 16-17 0.11787645166689487 2202 18-19 0.09682708610021251 2202 20-21 0.16558899481802314 2202 22-23 0.16566619981231412 2202 24-25 0.2723066782184418 2202 26-27 0.16766981313138274 2202 28-29 0.18591767848812424 2202 30-31 0.24349281597798011 2202 32-33 0.2977743397991546 2202 34-35 0.08597380503440633 2202 36-37 0.27326389903053183 2202 38-39 0.27756309166898774 2202 40-41 0.19437871791814842 2202 42-43 0.1498663740800552 2202 44-45 0.10157243060050547 2202 46-47 0.11710902218764119 2202 48-49 0.1349217232331128 2202 50-51 0.22905674919673658 2202 52-53 0.3083093104217127 2202 54-55 0.3664803767965523 2202 56-57 0.3535495548184784 2202 58-59 0.38154568241288445 2202 60-61 0.34986230116803796 2202 62-63 0.45632712091450145 2202 64-65 0.42280319587725046 2202 66-67 0.4447460671919643 2202 68-69 0.31648018585040205 2202 70-71 0.4011432599421809 2202 72-73 0.38266088706696166 2202 74-75 0.22453304271520835 2202 76-77 0.34994646670680396 2202 78-79 0.37126636434995675 2202 80-81 0.30036004635033464 2202 82-83 0.40126344727322305 2202 84-85 0.34500703632761187 2202 86-87 0.3215994393851709 2202 88-89 0.28518067053287055 2202 90-91 0.19269768254747888 2202 92-93 0.2233556285228886 2202 94-95 0.3485692368425717 2202 96-97 0.3213966574786049 2202 98-99 0.4140521766457361 2202 100-101 0.38341337998586766 1102 1 0.1876531438139466 1102 2 0.1699432580501714 1102 3 0.28548951356860286 1102 4 0.20405556544071857 1102 5 0.23604465661236418 1102 6 0.18906833407886836 1102 7 0.1994497055407436 1102 8 0.22369714439034283 1102 9 0.2339091034320191 1102 10-11 0.29527109054905054 1102 12-13 0.24336476726157485 1102 14-15 0.18468860067499548 1102 16-17 0.27006786206715105 1102 18-19 0.24554025328435358 1102 20-21 0.21347423390935205 1102 22-23 0.21516899645472165 1102 24-25 0.16161471386862303 1102 26-27 0.37144547621932844 1102 28-29 0.1971485066499099 1102 30-31 0.3233801316947691 1102 32-33 0.1893276794113703 1102 34-35 0.2753851954282496 1102 36-37 0.2920540564576939 1102 38-39 0.20354952793371694 1102 40-41 0.23284466013766547 1102 42-43 0.20308740870588338 1102 44-45 0.22777064192958818 1102 46-47 0.25508022856003976 1102 48-49 0.21282984097696556 1102 50-51 0.12442549317646723 1102 52-53 0.2360286505598026 1102 54-55 0.4215416650563455 1102 56-57 0.40387188479591174 1102 58-59 0.5259160311883093 1102 60-61 0.5439398648134883 1102 62-63 0.4918161553763625 1102 64-65 0.5397589485841365 1102 66-67 0.4689876275881417 1102 68-69 0.4800190436567817 1102 70-71 0.4662803791067489 1102 72-73 0.35759829194051207 1102 74-75 0.27261215762833757 1102 76-77 0.40895097376479583 1102 78-79 0.3560989757515287 1102 80-81 0.35510852217304034 1102 82-83 0.2791376073731371 1102 84-85 0.42459639036470875 1102 86-87 0.3840611048476781 1102 88-89 0.3089156732262488 1102 90-91 0.3082788426220624 1102 92-93 0.2341073157647635 1102 94-95 0.28736156280670855 1102 96-97 0.39720001491172496 1102 98-99 0.4819931514455362 1102 100-101 0.34183798291904566 2305 1 0.13760060504182903 2305 2 0.1106200261361927 2305 3 0.15625118043491426 2305 4 0.1860886705922482 2305 5 0.10972480162465814 2305 6 0.16797333446641005 2305 7 0.08824542704385863 2305 8 0.13106662099319522 2305 9 0.1549516523507677 2305 10-11 0.16269601691550406 2305 12-13 0.0937816423804847 2305 14-15 0.05663558275632852 2305 16-17 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-0.8471612933659642 1213 58-59 -1.1696431089932346 1213 60-61 -0.8049325821773223 1213 62-63 -0.8285345997361091 1213 64-65 -0.6735668581714478 1213 66-67 -0.6539786680295308 1213 68-69 -0.6063112052785868 1213 70-71 -0.5868679501369911 1213 72-73 -0.5982084737438527 1213 74-75 -0.5908179079761666 1213 76-77 -0.7752573221272172 1213 78-79 -0.8173317479443369 1213 80-81 -0.7632510708157731 1213 82-83 -0.6048740442542702 1213 84-85 -0.6140038253275755 1213 86-87 -0.7943246299043736 1213 88-89 -0.5842567426662697 1213 90-91 -0.549984605872627 1213 92-93 -0.49205550386743013 1213 94-95 -0.548855004321009 1213 96-97 -0.5673503343819455 1213 98-99 -0.777027278943681 1213 100-101 -1.0486381847004829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2445.0 3 225.0 4 260.0 5 338.0 6 779.0 7 4197.0 8 13731.0 9 30987.0 10 47058.0 11 41004.0 12 29486.0 13 21429.0 14 16787.0 15 14730.0 16 14105.0 17 14671.0 18 15719.0 19 16951.0 20 18968.0 21 21104.0 22 23061.0 23 25797.0 24 28931.0 25 33239.0 26 38411.0 27 45322.0 28 54664.0 29 67687.0 30 83410.0 31 107858.0 32 145650.0 33 206337.0 34 309219.0 35 509432.0 36 945586.0 37 1470101.0 38 400652.0 39 358.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.98144631275468 24.116904957783788 26.259852198066973 14.64179653139456 2 29.465814607529524 23.199273581837883 34.75551436784893 12.57939744278366 3 38.44418124291683 19.475911382602888 26.72260368441518 15.357303690065107 4 28.157761695809867 18.44124272873913 36.652949775490804 16.748045799960202 5 33.09167188246861 17.519174174270866 33.59427922948912 15.794874713771401 6 25.39574430597148 18.345244741572273 40.82201702801205 15.436993924444195 7 32.73837393597995 18.941000661812268 31.74436763692306 16.576257765284726 8 27.684058573060543 28.117893862290995 27.960847440024928 16.237200124623534 9 31.871627233479405 25.38430560600708 25.17156806241021 17.572499098103307 10-11 25.372910237082852 28.74570771255175 28.77437574350242 17.107006306862978 12-13 26.471872465104784 25.14643060787631 28.7512087003576 19.630488226661306 14-15 24.32975413975919 25.674976598166342 30.202630331829848 19.79263893024462 16-17 23.728573300802598 26.846755030241575 31.433056772250318 17.991614896705503 18-19 22.992484358986633 27.264788723911177 31.454649810257923 18.288077106844266 20-21 22.65205176756205 28.260941574843297 30.600273821681544 18.48673283591311 22-23 23.901490738677182 27.236580537544917 30.250978639315928 18.61095008446197 24-25 22.428603808741286 28.586257082120515 30.164198115212216 18.82094099392598 26-27 22.166246143790836 28.88623325915442 29.85242492623639 19.09509567081835 28-29 22.055488467054538 28.644602052960195 29.61048903404117 19.689420445944098 30-31 22.57734713013612 28.09353176112986 29.769647865508205 19.559473243225824 32-33 22.48136132715088 27.736171317304816 30.54240274674741 19.240064608796896 34-35 21.46423750353492 28.01243793539092 30.132039436371862 20.391285124702293 36-37 20.576953357951663 28.443770177985407 30.385162523200588 20.594113940862343 38-39 21.012749373828303 28.804647120858473 30.49196634037152 19.69063716494171 40-41 21.608210598907938 27.975377356675196 30.70962245869659 19.70678958572028 42-43 22.047872963208306 28.117068591479153 30.847832998822405 18.987225446490132 44-45 21.07198614805648 28.998668458421022 30.9409758126684 18.988369580854094 46-47 21.879153389519605 29.01129378597263 29.942495811624987 19.167057012882776 48-49 21.06669629873789 28.85305364320489 30.193936079627036 19.886313978430184 50-51 20.7649930382535 28.383251688101268 30.334517589776983 20.51723768386825 52-53 22.492741481020886 28.00969453455163 29.440921315822987 20.05664266860449 54-55 23.050807680800787 28.22313968772977 29.143512151725233 19.58254047974421 56-57 23.223373002533673 28.264365064427988 28.708665518279265 19.803596414759078 58-59 22.454864609434207 28.13040874563087 29.12156040711846 20.29316623781647 60-61 21.007512311965964 28.456633982461494 28.990823416877664 21.54503028869488 62-63 22.075807880974075 28.415712853285402 29.17392688193517 20.334552383805352 64-65 21.178765571925712 28.929272318104495 29.774560103956045 20.117402006013755 66-67 21.121196852044946 28.44260773973532 29.207959929065325 21.22823547915441 68-69 21.23385869599702 27.883481475694932 30.82455056179191 20.058109266516137 70-71 21.015681359125303 29.852891000031512 29.435579015828406 19.69584862501478 72-73 20.651010666926204 31.472246692737123 28.301595838043735 19.575146802292938 74-75 21.143183022592783 30.99274055944114 28.124768618851085 19.73930779911499 76-77 21.20224929880992 30.437368741335213 28.491735253586853 19.868646706268013 78-79 21.08689456037995 30.21792192054045 28.980430378401618 19.714753140677978 80-81 20.578306582335536 30.50941757627963 28.840661142276495 20.07161469910834 82-83 19.890426390550296 30.399326424320634 29.147472749128017 20.562774436001053 84-85 19.99207401937839 29.97822696292091 29.28092170269374 20.748777315006954 86-87 19.981600845075288 29.968109934690595 29.843645675095846 20.206643545138267 88-89 20.171494050726597 30.21879859280744 29.56169742862963 20.048009927836333 90-91 20.343582247622987 29.86025985486463 29.27160110001405 20.52455679749833 92-93 20.01369662851821 30.56993886436542 29.058868982960334 20.35749552415604 94-95 19.683770028102725 31.016635156041545 29.06500062078462 20.234594195071114 96-97 20.104885524647546 31.12857585675974 28.790331997810952 19.97620662078176 98-99 20.517869302188053 30.406035550874854 28.443175210301263 20.632919936635826 100-101 20.54728627281963 30.327495629730645 27.920228127644314 21.204989969805414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2016.0 1 1190.0 2 314.5 3 368.5 4 453.0 5 431.0 6 519.5 7 749.5 8 1012.5 9 1299.0 10 1567.5 11 1827.0 12 1902.0 13 1765.0 14 1645.5 15 1590.5 16 1526.5 17 1457.0 18 1522.0 19 1697.0 20 1974.5 21 2393.0 22 2980.5 23 3870.0 24 5163.0 25 7052.0 26 9908.5 27 13889.0 28 18410.5 29 22978.0 30 28456.0 31 35443.0 32 48051.0 33 69918.5 34 101915.0 35 140714.0 36 192281.0 37 254725.0 38 311610.0 39 336178.5 40 330300.0 41 307740.0 42 284711.0 43 285594.0 44 281719.0 45 263053.5 46 248839.5 47 221141.5 48 178710.5 49 148874.0 50 126787.0 51 107528.5 52 91880.5 53 75199.0 54 58433.5 55 43942.0 56 33212.5 57 24966.5 58 19192.0 59 14744.5 60 11017.0 61 8193.0 62 6091.5 63 4502.5 64 3278.5 65 2343.0 66 1647.0 67 1203.0 68 901.5 69 673.0 70 519.5 71 393.5 72 289.0 73 223.5 74 178.5 75 152.0 76 125.5 77 97.0 78 82.0 79 62.0 80 58.0 81 55.5 82 39.5 83 34.5 84 33.0 85 34.0 86 30.0 87 20.0 88 16.0 89 13.5 90 14.5 91 13.5 92 7.5 93 6.0 94 5.5 95 3.5 96 4.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17503721978331313 2 0.16665667501056383 3 0.13410946028669346 4 0.12834264977475213 5 0.14089272301117123 6 0.16171962140681548 7 0.1376359271465137 8 0.12838413762016176 9 0.12106153290535855 10-11 0.10829364848053877 12-13 0.13761518322380886 14-15 0.13337305103067218 16-17 0.12363377932075685 18-19 0.12163199077974124 20-21 0.11433012998764283 22-23 0.12645495280861305 24-25 0.4267647218063642 26-27 0.3104431752390582 28-29 0.393895976280569 30-31 0.2256731351057909 32-33 0.2629292202836565 34-35 0.27588380001281976 36-37 0.27299002279549667 38-39 0.29248931013803214 40-41 0.2848451746213041 42-43 0.34043888747023504 44-45 0.2873966771139976 46-47 0.31377257483318255 48-49 0.3129220740022848 50-51 0.31410447759645976 52-53 0.31740276130652695 54-55 0.2947918855582677 56-57 0.30741456252415367 58-59 0.307321214871982 60-61 0.28585125487248814 62-63 0.2835486794522526 64-65 0.29128616262115226 66-67 0.29068458886271237 68-69 0.2879775069497327 70-71 0.26252471379091247 72-73 0.252681722467473 74-75 0.2614771456943188 76-77 0.25795067883449857 78-79 0.254994669849061 80-81 0.2642257154527081 82-83 0.30485268807010785 84-85 0.3106298705434016 86-87 0.30057943999291387 88-89 0.30717600741304824 90-91 0.28335161218655674 92-93 0.2683537560709683 94-95 0.25427900451574453 96-97 0.2532521803418557 98-99 0.2746599085732351 100-101 0.27392349931721377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4820689.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.75830660918894 #Duplication Level Percentage of deduplicated Percentage of total 1 91.65344410365375 47.43827061705071 2 3.9609906787039293 4.100283400489948 3 1.1883331325736564 1.8451833188881581 4 0.6758233228531404 1.399178830314949 5 0.42860363815190106 1.1091899258639983 6 0.30694864443469533 0.9532285231155526 7 0.24475656496348772 0.8867729733794448 8 0.14923996093148983 0.6179526124988344 9 0.11829679894741389 0.5510557791725246 >10 1.019627795596813 10.187670910837802 >50 0.10092737873217845 3.6882323323800175 >100 0.12385622337120669 13.727505925849062 >500 0.022831339276218856 8.169321685174141 >1k 0.0061584396756710685 4.721729430501524 >5k 1.619781344419372E-4 0.6044237344835718 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8480 0.17590846453691578 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7147 0.1482568155713841 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6813 0.14132834538797254 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 6398 0.13271961746547017 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014520745893377481 0.0 0.0 2 0.001576538125566698 0.0 0.0 3 0.001680257739090823 0.0 0.0 4 0.001701001661795648 0.0 0.0 5 0.0019706726569583724 0.0 0.0 6 0.002136624038596972 0.0 0.0 7 0.002364807188350047 0.0 0.0 8 0.002613734260807947 0.0 0.0 9 0.003070100560314096 0.0 0.0 10-11 0.004885193796986282 0.0 0.0 12-13 0.006845494492592242 0.0 2.0743922704824975E-5 14-15 0.009054722260656102 0.0 2.0743922704824975E-5 16-17 0.011326181796834436 0.0 2.0743922704824975E-5 18-19 0.014147355284690633 0.0 2.0743922704824975E-5 20-21 0.017456010956110216 0.0 3.1115884057237466E-5 22-23 0.02114842919756906 0.0 4.148784540964995E-5 24-25 0.025432049236115417 0.0 4.148784540964995E-5 26-27 0.030099431844701034 0.0 4.148784540964995E-5 28-29 0.03548247978660311 0.0 4.148784540964995E-5 30-31 0.054753583979385526 0.0 4.148784540964995E-5 32-33 0.061381267283577096 0.0 4.148784540964995E-5 34-35 0.06805043843317833 0.0 4.148784540964995E-5 36-37 0.07490630488712298 0.0 4.148784540964995E-5 38-39 0.08273713570819441 0.0 6.223176811447492E-5 40-41 0.090547222606561 0.0 6.223176811447492E-5 42-43 0.09918706641312061 0.0 6.223176811447492E-5 44-45 0.10936196049983726 0.0 6.223176811447492E-5 46-47 0.11979615362036422 0.0 6.223176811447492E-5 48-49 0.13145423818047586 0.0 6.223176811447492E-5 50-51 0.1441184029917715 0.0 6.223176811447492E-5 52-53 0.15737376960015464 0.0 6.223176811447492E-5 54-55 0.17155224076890252 0.0 6.223176811447492E-5 56-57 0.18698571926129232 0.0 8.29756908192999E-5 58-59 0.20480474886473699 0.0 8.29756908192999E-5 60-61 0.22375432225559458 0.0 8.29756908192999E-5 62-63 0.24340918901841624 0.0 8.29756908192999E-5 64-65 0.26598894888261826 0.0 8.29756908192999E-5 66-67 0.2898859478385766 0.0 1.0371961352412487E-4 68-69 0.3165522604756291 0.0 1.1409157487653735E-4 70-71 0.34589453914160406 0.0 1.3483549758136232E-4 72-73 0.37719711850318494 0.0 1.452074589337748E-4 74-75 0.41200542080188124 0.0 1.452074589337748E-4 76-77 0.44867030418265935 0.0 1.555794202861873E-4 78-79 0.49019963743771894 0.0 1.659513816385998E-4 80-81 0.5363859813400118 0.0 1.659513816385998E-4 82-83 0.5869389209716702 0.0 1.659513816385998E-4 84-85 0.6434038785742038 0.0 1.659513816385998E-4 86-87 0.704712542128314 0.0 1.659513816385998E-4 88-89 0.7718917358078896 0.0 1.659513816385998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1780 0.0 77.50764 9 CGGGAGA 2400 0.0 72.70617 4 AGAGCGG 2080 0.0 67.2383 8 TCGGGAG 2785 0.0 63.50652 3 AAGCGGC 755 0.0 62.166977 9 GAGAGCG 2660 0.0 59.706436 7 CGGAAAG 985 0.0 59.692554 5 TCGGGAA 740 0.0 57.02864 3 GATCGGG 4185 0.0 52.697678 1 GATCCGG 540 0.0 50.06279 1 TTGTGTT 66370 0.0 49.9433 1 AAGCGTC 470 0.0 49.427628 9 ATCGGGA 4245 0.0 49.374912 2 ATTCGGA 525 0.0 47.871647 2 GTTCGGA 385 0.0 43.109116 2 GAGCGTC 6945 0.0 43.075333 9 AGAGCGT 7055 0.0 43.074375 8 GGCGCCG 2225 0.0 42.267883 56-57 AAGGGGC 2150 0.0 42.11787 9 CCGGATC 1250 0.0 41.798893 60-61 >>END_MODULE