FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4820689
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC84800.17590846453691578No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG71470.1482568155713841No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA68130.14132834538797254No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT63980.13271961746547017Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC17800.077.507649
CGGGAGA24000.072.706174
AGAGCGG20800.067.23838
TCGGGAG27850.063.506523
AAGCGGC7550.062.1669779
GAGAGCG26600.059.7064367
CGGAAAG9850.059.6925545
TCGGGAA7400.057.028643
GATCGGG41850.052.6976781
GATCCGG5400.050.062791
TTGTGTT663700.049.94331
AAGCGTC4700.049.4276289
ATCGGGA42450.049.3749122
ATTCGGA5250.047.8716472
GTTCGGA3850.043.1091162
GAGCGTC69450.043.0753339
AGAGCGT70550.043.0743758
GGCGCCG22250.042.26788356-57
AAGGGGC21500.042.117879
CCGGATC12500.041.79889360-61
Produced by FastQC (version 0.11.2)