##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34311-1.34200000035761.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2602899 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.858507763843313 33.0 33.0 33.0 27.0 33.0 2 31.836014382425134 33.0 33.0 33.0 27.0 33.0 3 31.780441730547363 33.0 33.0 33.0 27.0 33.0 4 35.51826636377362 37.0 37.0 37.0 33.0 37.0 5 35.516443012195246 37.0 37.0 37.0 33.0 37.0 6 35.56058110591306 37.0 37.0 37.0 33.0 37.0 7 35.46400340543371 37.0 37.0 37.0 33.0 37.0 8 35.404369512608824 37.0 37.0 37.0 33.0 37.0 9 35.36557392353679 37.0 37.0 37.0 33.0 37.0 10-11 35.287929343397494 37.0 37.0 37.0 33.0 37.0 12-13 35.254036172744314 37.0 37.0 37.0 33.0 37.0 14-15 36.83888944596006 40.0 37.0 40.0 33.0 40.0 16-17 36.808486806441586 40.0 37.0 40.0 33.0 40.0 18-19 36.844756942163336 40.0 37.0 40.0 33.0 40.0 20-21 36.81726432719825 40.0 37.0 40.0 33.0 40.0 22-23 36.81647943312437 40.0 37.0 40.0 33.0 40.0 24-25 36.559225886213795 40.0 37.0 40.0 33.0 40.0 26-27 36.4438226377589 40.0 37.0 40.0 33.0 40.0 28-29 36.35570569584144 40.0 37.0 40.0 33.0 40.0 30-31 36.41789654535194 40.0 37.0 40.0 33.0 40.0 32-33 36.31153782762989 37.0 37.0 40.0 33.0 40.0 34-35 36.25053046622247 37.0 37.0 40.0 33.0 40.0 36-37 36.18146324540445 37.0 37.0 40.0 33.0 40.0 38-39 36.13325257722255 37.0 37.0 40.0 33.0 40.0 40-41 36.00635406905916 37.0 37.0 40.0 30.0 40.0 42-43 35.87141971317366 37.0 37.0 40.0 27.0 40.0 44-45 35.779588066997604 37.0 37.0 40.0 27.0 40.0 46-47 35.67239086111294 37.0 37.0 40.0 27.0 40.0 48-49 35.546140476445686 37.0 37.0 40.0 27.0 40.0 50-51 34.40655361579531 37.0 35.0 38.5 27.0 38.5 52-53 34.89782527097671 37.0 35.0 38.5 27.0 40.0 54-55 35.64937133557622 37.0 37.0 40.0 27.0 40.0 56-57 35.71322321765078 37.0 37.0 40.0 27.0 40.0 58-59 35.468117472095535 37.0 37.0 40.0 27.0 40.0 60-61 35.25545920145191 37.0 35.0 40.0 27.0 40.0 62-63 35.0322010189408 37.0 33.0 40.0 27.0 40.0 64-65 34.7646643223575 37.0 33.0 38.5 27.0 40.0 66-67 34.53123133091219 37.0 33.0 37.0 27.0 40.0 68-69 34.300488801140574 37.0 33.0 37.0 27.0 40.0 70-71 33.97580659103561 37.0 33.0 37.0 27.0 40.0 72-73 33.73295352604922 37.0 33.0 37.0 27.0 38.5 74-75 33.48229915951407 37.0 33.0 37.0 27.0 37.0 76-77 33.21908744826442 37.0 33.0 37.0 27.0 37.0 78-79 32.97960408759618 37.0 33.0 37.0 27.0 37.0 80-81 32.76317694232469 37.0 33.0 37.0 27.0 37.0 82-83 32.60447466459513 37.0 33.0 37.0 24.5 37.0 84-85 32.430910304241536 35.0 33.0 37.0 22.0 37.0 86-87 32.31175969563168 33.0 33.0 37.0 22.0 37.0 88-89 32.195682775244066 33.0 33.0 37.0 22.0 37.0 90-91 32.063278482953045 33.0 33.0 37.0 22.0 37.0 92-93 31.920874378913666 33.0 33.0 37.0 22.0 37.0 94-95 31.772901291982517 33.0 33.0 37.0 22.0 37.0 96-97 31.645090723842912 33.0 33.0 37.0 22.0 37.0 98-99 31.478970179019623 33.0 33.0 37.0 22.0 37.0 100-101 30.220538906811214 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.4280844945104043 2306 2 0.3880399187917227 2306 3 0.45020803321628833 2306 4 0.5594525183627113 2306 5 0.5564406895383911 2306 6 0.5270430453391057 2306 7 0.590395670170885 2306 8 0.49297123861573056 2306 9 0.47832192440907306 2306 10-11 0.5696867262818728 2306 12-13 0.528661893338132 2306 14-15 0.8144135393336143 2306 16-17 0.8108489128733893 2306 18-19 0.7374328265867689 2306 20-21 0.5933992228268536 2306 22-23 0.7307342064005198 2306 24-25 0.8468010503573211 2306 26-27 0.8792326199405025 2306 28-29 0.7560536810371801 2306 30-31 0.7417963511298638 2306 32-33 0.7587825201593219 2306 34-35 0.8241432060888911 2306 36-37 0.6605135054094333 2306 38-39 0.8450211558028613 2306 40-41 0.8177433908588441 2306 42-43 0.7462400860075178 2306 44-45 0.7970400993918858 2306 46-47 0.6452406913624884 2306 48-49 0.6824586298710997 2306 50-51 0.75215222954985 2306 52-53 0.8707076410724426 2306 54-55 0.9967863410203535 2306 56-57 0.7809177073737814 2306 58-59 1.009136324750557 2306 60-61 0.9901716389300432 2306 62-63 0.8950082835334783 2306 64-65 1.041177200512486 2306 66-67 0.9076346861221083 2306 68-69 0.980898817952017 2306 70-71 0.9905151851158891 2306 72-73 0.7901005966628318 2306 74-75 0.863678523852677 2306 76-77 0.949939473155105 2306 78-79 0.8667509165296039 2306 80-81 0.9970528459226244 2306 82-83 0.9713300308112451 2306 84-85 0.8467246280486478 2306 86-87 0.9369973473824871 2306 88-89 0.8367128803511932 2306 90-91 0.7992225217349969 2306 92-93 0.7868636703731084 2306 94-95 0.7254031580592049 2306 96-97 0.7295146432074802 2306 98-99 1.0829274370945932 2306 100-101 0.9950203152382429 2307 1 0.37560720273872406 2307 2 0.31416185111364925 2307 3 0.4191252021091394 2307 4 0.43009662961674877 2307 5 0.3676919244642036 2307 6 0.4203197993679382 2307 7 0.36312945549133957 2307 8 0.3796702735283617 2307 9 0.3965711183276497 2307 10-11 0.4602493077632914 2307 12-13 0.5436209251799724 2307 14-15 0.7731244879500494 2307 16-17 0.7763862432548763 2307 18-19 0.7223970250858756 2307 20-21 0.6995775818349372 2307 22-23 0.7086961831185903 2307 24-25 0.7697243126691831 2307 26-27 0.7816392402085555 2307 28-29 0.7778019925980502 2307 30-31 0.9296858486063329 2307 32-33 0.7523009111304262 2307 34-35 0.764702373392673 2307 36-37 0.8523366091692495 2307 38-39 0.6258163473777003 2307 40-41 0.6425427931212013 2307 42-43 0.554646896522236 2307 44-45 0.5766428943600559 2307 46-47 0.650012251302222 2307 48-49 0.6840184946143992 2307 50-51 0.6136996295845094 2307 52-53 0.6678227991133454 2307 54-55 0.5958111097936936 2307 56-57 0.5661270556684457 2307 58-59 0.4489202961140961 2307 60-61 0.5650260035318482 2307 62-63 0.5294712294438284 2307 64-65 0.659062833850335 2307 66-67 0.8719064025614287 2307 68-69 0.7792926151214488 2307 70-71 0.45792103768735615 2307 72-73 0.5278524810717968 2307 74-75 0.6895797384710463 2307 76-77 0.7387609424513855 2307 78-79 0.7552645967249703 2307 80-81 0.7669080650555955 2307 82-83 0.5877590141401896 2307 84-85 0.6472742909134084 2307 86-87 0.638532109039339 2307 88-89 0.5839384169790023 2307 90-91 0.6265428896136171 2307 92-93 0.6260874797822709 2307 94-95 0.57262285308828 2307 96-97 0.6598622617495096 2307 98-99 0.5166795630615688 2307 100-101 0.6303956162483217 2201 1 0.3466627362040633 2201 2 0.32650568615958875 2201 3 0.3255449786189182 2201 4 0.4768907781531766 2201 5 0.45560314734958496 2201 6 0.5125080026526732 2201 7 0.4803642668958119 2201 8 0.47829985015641796 2201 9 0.47758514685027365 2201 10-11 0.46845946850159237 2201 12-13 0.42960377285367457 2201 14-15 0.5848191060325831 2201 16-17 0.4583034029146802 2201 18-19 0.4717020241809564 2201 20-21 0.5689032829414842 2201 22-23 0.573497818557307 2201 24-25 0.7698484440021574 2201 26-27 0.6780945337539279 2201 28-29 0.6153472130707698 2201 30-31 0.7039410774284818 2201 32-33 0.4005022851196216 2201 34-35 0.5739678523007754 2201 36-37 0.6591046598498167 2201 38-39 0.5184859716668058 2201 40-41 0.579886914106531 2201 42-43 0.39225620105710135 2201 44-45 0.5784402756935307 2201 46-47 0.5637460208781349 2201 48-49 0.5626013992068621 2201 50-51 0.5861029467504935 2201 52-53 0.7091785487488522 2201 54-55 0.8725430782878831 2201 56-57 0.8372332508647986 2201 58-59 0.8892198529416859 2201 60-61 0.7235773740561413 2201 62-63 0.6261535588607927 2201 64-65 0.6141229181250836 2201 66-67 0.7239975277429949 2201 68-69 0.7865605214867202 2201 70-71 0.7969304360103351 2201 72-73 0.8623250305544516 2201 74-75 0.8886613348067058 2201 76-77 0.77629839210082 2201 78-79 0.7883223854110497 2201 80-81 0.7820752992278415 2201 82-83 0.6173791386213736 2201 84-85 0.6116805562826428 2201 86-87 0.6493367491963937 2201 88-89 0.5158123493317959 2201 90-91 0.662466800134105 2201 92-93 0.7557241551078491 2201 94-95 0.6089316043808886 2201 96-97 0.6103469794667831 2201 98-99 0.5206744726677179 2201 100-101 0.4628622168461689 1101 1 0.4057057222035745 1101 2 0.37107271333531244 1101 3 0.33538788933139685 1101 4 0.3371484914543643 1101 5 0.43845507802760153 1101 6 0.406325489513641 1101 7 0.3392847666858785 1101 8 0.42335711173542023 1101 9 0.36217504970104386 1101 10-11 0.4352720761837716 1101 12-13 0.4528083943191632 1101 14-15 0.6197839962148635 1101 16-17 0.5753560604021217 1101 18-19 0.5671646774696981 1101 20-21 0.5182744914431296 1101 22-23 0.5348302542375691 1101 24-25 0.6045624256726541 1101 26-27 0.6593054967040217 1101 28-29 0.5677120945660974 1101 30-31 0.4942956503917557 1101 32-33 0.43925715343971916 1101 34-35 0.5130173535241838 1101 36-37 0.6178712555729291 1101 38-39 0.47446897417155043 1101 40-41 0.5352039159452744 1101 42-43 0.2809312940800197 1101 44-45 0.3884910944400062 1101 46-47 0.4625741492714823 1101 48-49 0.49856065734002897 1101 50-51 0.325055153963568 1101 52-53 0.5194846194897096 1101 54-55 0.6764425121873217 1101 56-57 0.7444176606579092 1101 58-59 0.6573097340750493 1101 60-61 0.6115488228971557 1101 62-63 0.6279093393122324 1101 64-65 0.7065962419027514 1101 66-67 0.7157763286522396 1101 68-69 0.6056376762160554 1101 70-71 0.6517839882551968 1101 72-73 0.6701417066319948 1101 74-75 0.7137728899182676 1101 76-77 0.6806803869176008 1101 78-79 0.7782598392615512 1101 80-81 0.6535846594328518 1101 82-83 0.5844852209448561 1101 84-85 0.688860477810401 1101 86-87 0.7547792776824025 1101 88-89 0.7701969502381374 1101 90-91 0.6184451695907356 1101 92-93 0.4124284652904251 1101 94-95 0.6256703728587958 1101 96-97 0.5888979936699741 1101 98-99 0.6185118526790099 1101 100-101 0.6821286026342968 2304 1 0.3691739026131806 2304 2 0.2900912219416689 2304 3 0.2033015158350402 2304 4 0.3067073274123331 2304 5 0.3012237627051775 2304 6 0.17843890754896563 2304 7 0.22420067507250252 2304 8 0.1537968672077099 2304 9 0.29177839646274606 2304 10-11 0.22320443666493617 2304 12-13 0.24885848305905967 2304 14-15 0.3853910897720638 2304 16-17 0.46051945449175946 2304 18-19 0.3332926601161148 2304 20-21 0.33812377448113295 2304 22-23 0.3569910002161052 2304 24-25 0.6126777593628319 2304 26-27 0.5077009949151403 2304 28-29 0.493555968696306 2304 30-31 0.4282196439686743 2304 32-33 0.2183193277079596 2304 34-35 0.33527754452624237 2304 36-37 0.2743837777579117 2304 38-39 0.36585711964863066 2304 40-41 0.34567677280642073 2304 42-43 0.30033222937032633 2304 44-45 0.32713168378557356 2304 46-47 0.4261498035521214 2304 48-49 0.4079118701185749 2304 50-51 0.3111991943050114 2304 52-53 0.24798590128816755 2304 54-55 0.4641847157538237 2304 56-57 0.543184880422956 2304 58-59 0.5551785680610237 2304 60-61 0.47969894825985904 2304 62-63 0.4767624818313152 2304 64-65 0.5426452037868543 2304 66-67 0.6256642065010709 2304 68-69 0.536377034471684 2304 70-71 0.5612357729130579 2304 72-73 0.5214623816186119 2304 74-75 0.6266334133427804 2304 76-77 0.6683094823341165 2304 78-79 0.43766781740054483 2304 80-81 0.5675804900664261 2304 82-83 0.8099014533025155 2304 84-85 0.5838869589214752 2304 86-87 0.5778726376846564 2304 88-89 0.7648395960955412 2304 90-91 0.6550895339032152 2304 92-93 0.5976989106801724 2304 94-95 0.5232549574715755 2304 96-97 0.5632685038539336 2304 98-99 0.4097860448642656 2304 100-101 0.207391160033513 2202 1 0.3067388067660666 2202 2 0.2637212412779597 2202 3 0.22055448743916983 2202 4 0.29373778451424926 2202 5 0.2853493602159958 2202 6 0.19964158518267539 2202 7 0.22409172443401104 2202 8 0.28529047249230644 2202 9 0.3255751789835273 2202 10-11 0.26015072694477226 2202 12-13 0.34493920468526795 2202 14-15 0.4880586627248249 2202 16-17 0.3482666791950919 2202 18-19 0.27286603493575967 2202 20-21 0.3299269566250089 2202 22-23 0.2956041861879726 2202 24-25 0.44063341350835117 2202 26-27 0.308007970700622 2202 28-29 0.4707901929383027 2202 30-31 0.3021671906162311 2202 32-33 0.44711517776833176 2202 34-35 0.4423362699019293 2202 36-37 0.37227273644500514 2202 38-39 0.45971490471159626 2202 40-41 0.4228503871211515 2202 42-43 0.42816347366513696 2202 44-45 0.31126537897899453 2202 46-47 0.47453689241069696 2202 48-49 0.41973301439473687 2202 50-51 0.45204169683245965 2202 52-53 0.5689004977291106 2202 54-55 0.693282143144593 2202 56-57 0.6020506159379195 2202 58-59 0.6592342801172464 2202 60-61 0.5985265510513571 2202 62-63 0.6642576968370548 2202 64-65 0.5969840893494265 2202 66-67 0.5314012919242543 2202 68-69 0.5986170497836696 2202 70-71 0.5628823017064448 2202 72-73 0.5833919857646279 2202 74-75 0.42651955501786887 2202 76-77 0.5224456804475963 2202 78-79 0.5432407407130313 2202 80-81 0.34227596812415584 2202 82-83 0.5997288008943187 2202 84-85 0.5445712376387988 2202 86-87 0.46098013564797213 2202 88-89 0.45704128237659347 2202 90-91 0.4408457102265686 2202 92-93 0.5812373035110241 2202 94-95 0.4283623867272759 2202 96-97 0.4519661020322019 2202 98-99 0.45721785256148095 2202 100-101 0.346021768292168 1102 1 0.2373366843095006 1102 2 0.14461855132273982 1102 3 0.28044098770926595 1102 4 0.3158011096962028 1102 5 0.25532207923349404 1102 6 0.2963016494256152 1102 7 0.2626359384067527 1102 8 0.18071969780147157 1102 9 0.28401379290050954 1102 10-11 0.327506098045518 1102 12-13 0.3254633132128504 1102 14-15 0.22631951461173827 1102 16-17 0.2985850864039321 1102 18-19 0.3459494442647326 1102 20-21 0.33481962367503826 1102 22-23 0.31054492895096786 1102 24-25 0.35243510070836237 1102 26-27 0.3402153100400298 1102 28-29 0.4078838098854263 1102 30-31 0.37981043513632073 1102 32-33 0.30442750726218293 1102 34-35 0.2618480777849541 1102 36-37 0.2806712711328956 1102 38-39 0.07880220191940879 1102 40-41 0.3570821147682679 1102 42-43 0.2692674337640426 1102 44-45 0.388583342799258 1102 46-47 0.503005827135766 1102 48-49 0.254052935707783 1102 50-51 0.12447754586349191 1102 52-53 0.3382756102704292 1102 54-55 0.6025305704704991 1102 56-57 0.6092975988626179 1102 58-59 0.5472441043688505 1102 60-61 0.5727000379636493 1102 62-63 0.5331746089163971 1102 64-65 0.5303055150857077 1102 66-67 0.5534980164637773 1102 68-69 0.4680562503676242 1102 70-71 0.4868818215301829 1102 72-73 0.5204290658282176 1102 74-75 0.5039718907462785 1102 76-77 0.6641509257925051 1102 78-79 0.557499846152389 1102 80-81 0.5520287741542447 1102 82-83 0.32622426923076375 1102 84-85 0.48949821405788185 1102 86-87 0.6737659905843927 1102 88-89 0.5481999399741255 1102 90-91 0.4450787157621434 1102 92-93 0.6191266964559148 1102 94-95 0.5803493096686658 1102 96-97 0.4362750838455227 1102 98-99 0.6107797730342277 1102 100-101 0.5172043185315793 2305 1 0.09870155418080984 2305 2 0.08939311564924779 2305 3 0.13214544059177769 2305 4 0.11798671547771988 2305 5 0.14582628402451547 2305 6 0.14285626673964913 2305 7 0.1624285267253569 2305 8 0.04498715509545548 2305 9 0.13193599207314577 2305 10-11 0.15164306170592567 2305 12-13 0.11925507617409181 2305 14-15 0.010107884129702427 2305 16-17 0.04042750472174106 2305 18-19 0.08132797577204087 2305 20-21 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-0.7241744908050229 1213 90-91 -0.7529476932956847 1213 92-93 -0.6568715750786112 1213 94-95 -0.7831835076554334 1213 96-97 -0.7087932259014593 1213 98-99 -0.7869119729267702 1213 100-101 -1.2185818266887196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1229.0 3 106.0 4 114.0 5 176.0 6 372.0 7 1517.0 8 4721.0 9 8690.0 10 12207.0 11 10922.0 12 8529.0 13 6957.0 14 6610.0 15 6895.0 16 7123.0 17 7410.0 18 7927.0 19 8711.0 20 9711.0 21 10988.0 22 12015.0 23 13486.0 24 15152.0 25 17824.0 26 20722.0 27 24291.0 28 29391.0 29 35848.0 30 44813.0 31 59104.0 32 80421.0 33 116098.0 34 175530.0 35 293722.0 36 543962.0 37 813573.0 38 185922.0 39 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.609189890458545 23.199212431452406 28.18086059105357 13.010737087035483 2 32.54634334811185 20.441292653370496 35.52041431651667 11.49194968200099 3 40.310579411755654 16.93783795428596 27.761915533356106 14.989667100602277 4 31.683902625169065 15.969162995594713 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22.07793435381039 27.451739537216547 31.6506071742045 18.81971893476856 26-27 21.760431242415475 27.86325672342241 31.834244664866485 18.542067369295637 28-29 20.987258628436766 28.493579174115364 31.139260279203235 19.37990191824463 30-31 20.785712264235396 28.75018506157906 30.653571077219144 19.810531596966396 32-33 19.937653425306024 28.947303547237375 31.916662204902448 19.19838082255416 34-35 20.247817038846808 29.165385744433475 32.14906775828208 18.437729458437637 36-37 20.30969264953788 30.207880690205652 31.446755299969492 18.03567136028698 38-39 20.727936989136776 29.913958052865077 30.371100447644682 18.98700451035347 40-41 21.19867272193139 29.082088006819397 29.88781707064529 19.83142220060392 42-43 20.89349137631117 29.32468603327275 30.961185256800565 18.82063733361551 44-45 20.124659622977365 29.527647716644495 31.883003736321108 18.464688924057036 46-47 20.4652920330484 29.301038750377916 31.47847011745219 18.755199099121494 48-49 20.775406237155902 30.26976239782289 29.82134987757342 19.133481487447785 50-51 20.46113208477918 29.82912284163485 29.867892868319107 19.841852205266868 52-53 20.471818841728933 29.447102530212028 30.103420294457532 19.977658333601507 54-55 21.454207495177258 29.301604408506215 29.83288157948225 19.411306516834276 56-57 21.830796763281633 29.391835910494674 28.98102254589063 19.796344780333058 58-59 21.13535468754817 29.270663131608966 30.139289237599037 19.454692943243828 60-61 20.108101922798706 29.61319600148487 30.330780402413627 19.947921673302798 62-63 20.89820374276923 29.66339271799896 29.727077623732395 19.711325915499415 64-65 20.66433971940237 29.770720304355148 29.64403409276991 19.92090588347257 66-67 21.13960881274261 29.327851232711666 29.444424858174536 20.08811509637119 68-69 21.998050049500076 29.241648029889223 29.386016030743022 19.374285889867675 70-71 21.1210301831078 30.665442789453955 29.187834465342714 19.025692562095525 72-73 20.241092764886893 31.30608636271988 28.943822501002835 19.508998371390394 74-75 21.199858173157015 30.253992154071252 28.719454217027813 19.82669545574392 76-77 21.50782095577048 29.413649792198687 29.142834032423938 19.93569521960689 78-79 21.476336616426558 29.341220614312807 29.701459597616946 19.480983171643686 80-81 19.807340520314643 30.092836014873175 30.025831223423904 20.073992241388275 82-83 19.18012508525913 29.806919380508123 30.23128827027048 20.781667263962266 84-85 19.082923355225383 29.104546922485813 30.764659591637912 21.047870130650896 86-87 19.15353520212536 29.167717511365982 31.897036550514045 19.781710735994608 88-89 19.56670773331011 29.815134593130015 31.226076303801705 19.392081369758174 90-91 19.886879379614633 29.613649988364692 30.310631897387374 20.1888387346333 92-93 19.11246936607321 30.620752564158867 30.406729178752673 19.860048891015257 94-95 18.798889593939176 30.922536370805524 30.74775237136015 19.530821663895146 96-97 19.49424320487882 31.6498845598262 29.79407953384085 19.061792701454127 98-99 20.157595339132005 30.542981413812292 29.067419978731234 20.23200326832447 100-101 19.60232558766662 30.58632407446288 28.647050235051235 21.16430010281927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1813.0 1 1111.5 2 383.0 3 481.5 4 563.0 5 530.5 6 604.5 7 802.0 8 1012.5 9 1159.5 10 1277.5 11 1323.0 12 1310.5 13 1280.0 14 1192.5 15 1106.5 16 1043.0 17 1019.5 18 1072.0 19 1135.0 20 1262.0 21 1455.5 22 1693.0 23 2102.5 24 2660.5 25 3361.0 26 4741.0 27 6933.5 28 9108.5 29 11081.0 30 13569.5 31 16932.5 32 24018.0 33 35293.0 34 51338.5 35 77820.0 36 122893.5 37 181205.5 38 229786.0 39 237583.5 40 214009.0 41 186078.5 42 161532.5 43 150199.5 44 134429.5 45 115581.0 46 111080.0 47 99511.0 48 75677.5 49 58165.5 50 47299.5 51 40036.0 52 34330.5 53 28406.0 54 22874.0 55 16976.0 56 12170.0 57 9191.5 58 7280.0 59 5674.5 60 4331.5 61 3434.5 62 2638.0 63 1970.0 64 1513.5 65 1064.5 66 727.0 67 558.5 68 411.5 69 311.0 70 244.5 71 210.5 72 170.0 73 113.5 74 81.5 75 64.5 76 58.0 77 52.0 78 41.5 79 44.0 80 39.0 81 28.5 82 25.0 83 18.0 84 13.0 85 13.5 86 13.5 87 9.5 88 7.0 89 6.0 90 6.0 91 7.5 92 6.5 93 4.0 94 2.0 95 2.0 96 2.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17372936867700206 2 0.16543092912940532 3 0.13196824002775367 4 0.1265896218024595 5 0.1392293746319008 6 0.160974359742733 7 0.13523382966453942 8 0.1262438534879763 9 0.11932848719831235 10-11 0.10638138475599707 12-13 0.13584852889028734 14-15 0.13137275015281039 16-17 0.12203700566176406 18-19 0.11988555837164638 20-21 0.11197130584014209 22-23 0.1239003126898124 24-25 0.4216068314598453 26-27 0.30650440143854984 28-29 0.39048768315635757 30-31 0.22246349166832827 32-33 0.2599409350881459 34-35 0.27327222454655364 36-37 0.2702755658210326 38-39 0.2895617540288732 40-41 0.28281927189645084 42-43 0.3378732713024977 44-45 0.28550858100909793 46-47 0.3111338549824638 48-49 0.3103654809502789 50-51 0.31161408875257934 52-53 0.31486046903856046 54-55 0.2936533457502577 56-57 0.30446821025325993 58-59 0.30500607207578934 60-61 0.2837413207350727 62-63 0.28074466200955167 64-65 0.28852444908542363 66-67 0.2898114755893333 68-69 0.2861616989364551 70-71 0.26040195950745687 72-73 0.25020179423020256 74-75 0.2603059127534338 76-77 0.2562143210320493 78-79 0.25229561346790635 80-81 0.2618618701686082 82-83 0.30231676296314225 84-85 0.3090784544463692 86-87 0.29830200864497625 88-89 0.30466030376130615 90-91 0.2824158755295538 92-93 0.26589583383757875 94-95 0.25170012359296307 96-97 0.25097016826238744 98-99 0.27175468583298856 100-101 0.2720812447966671 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2602899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.52650927150673 #Duplication Level Percentage of deduplicated Percentage of total 1 86.52225466981827 35.92967210737875 2 6.004671475667687 4.987060914133324 3 1.9926797972619807 2.482471082284314 4 1.1055987467672026 1.8364662643277785 5 0.7139653550106815 1.4824244467192829 6 0.5453490174714405 1.3587864618140915 7 0.432008744113833 1.2557870582470547 8 0.3086969933805719 1.0255286846163656 9 0.2890778704237868 1.0803955379706722 >10 1.6855827034110469 13.752801081681806 >50 0.1802077595454535 5.230883177871625 >100 0.18937058636290918 17.43412892754898 >500 0.023838854790756488 6.568047728669243 >1k 0.006229082185896684 4.525940020247067 >5k 4.6834378849031945E-4 1.049606506489464 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5830 0.2239810303818934 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5504 0.21145653365727984 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5467 0.2100350416977378 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5097 0.19582012210231747 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5086 0.19539751638461578 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4548 0.17472825491884242 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 4042 0.15528839190456487 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3944 0.15152335914685897 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3820 0.14675944014731268 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3812 0.14645209053443872 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 3218 0.12363138177854768 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 2918 0.11210577129577444 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2801 0.10761078320749287 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 2799 0.10753394580427439 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2772 0.1064966408608248 No Hit TTGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA 2701 0.10376891304656845 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.8418701609244153E-4 0.0 0.0 2 3.8418701609244153E-4 0.0 0.0 3 4.99443120920174E-4 0.0 0.0 4 4.99443120920174E-4 0.0 0.0 5 5.378618225294182E-4 0.0 0.0 6 5.762805241386624E-4 0.0 0.0 7 7.683740321848831E-4 0.0 0.0 8 0.0011141423466680806 0.0 0.0 9 0.001229398451495813 0.0 0.0 10-11 0.001767260274025231 0.0 0.0 12-13 0.002746937165060957 0.0 0.0 14-15 0.0037650327577059273 0.0 3.8418701609244156E-5 16-17 0.004956012507592496 0.0 3.8418701609244156E-5 18-19 0.006185410959088309 0.0 3.8418701609244156E-5 20-21 0.006992203692882436 0.0 3.8418701609244156E-5 22-23 0.007971880583918163 0.0 3.8418701609244156E-5 24-25 0.009374163192655573 0.0 3.8418701609244156E-5 26-27 0.010718817748979119 0.0 3.8418701609244156E-5 28-29 0.012005844252888798 0.0 3.8418701609244156E-5 30-31 0.018671488982092657 0.0 3.8418701609244156E-5 32-33 0.02082293627221033 0.0 3.8418701609244156E-5 34-35 0.022532568493821695 0.0 3.8418701609244156E-5 36-37 0.024587969029916258 0.0 3.8418701609244156E-5 38-39 0.02618234514669989 0.0 3.8418701609244156E-5 40-41 0.028122489577966724 0.0 3.8418701609244156E-5 42-43 0.030658123884176835 0.0 3.8418701609244156E-5 44-45 0.033309014295214684 0.0 3.8418701609244156E-5 46-47 0.03624804496832186 0.0 3.8418701609244156E-5 48-49 0.03903340083499206 0.0 3.8418701609244156E-5 50-51 0.042414246576605544 0.0 3.8418701609244156E-5 52-53 0.04660188505201316 0.0 3.8418701609244156E-5 54-55 0.05115450119270859 0.0 3.8418701609244156E-5 56-57 0.05616814175271495 0.0 3.8418701609244156E-5 58-59 0.06164280673203224 0.0 3.8418701609244156E-5 60-61 0.06811635795318989 0.0 3.8418701609244156E-5 62-63 0.07624191334354502 0.0 7.683740321848831E-5 64-65 0.08454035289114176 0.0 7.683740321848831E-5 66-67 0.09416423764425742 0.0 7.683740321848831E-5 68-69 0.10547854526817982 0.0 7.683740321848831E-5 70-71 0.11742676146865476 0.0 7.683740321848831E-5 72-73 0.13258293925350156 0.0 7.683740321848831E-5 74-75 0.1499097736792707 0.0 7.683740321848831E-5 76-77 0.16979145176205454 0.0 7.683740321848831E-5 78-79 0.19213192674783003 0.0 7.683740321848831E-5 80-81 0.21794929422924209 0.0 7.683740321848831E-5 82-83 0.24741643836353233 0.0 7.683740321848831E-5 84-85 0.28189722305782894 0.0 7.683740321848831E-5 86-87 0.32265946546523705 0.0 7.683740321848831E-5 88-89 0.37035628351311367 0.0 7.683740321848831E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGTGC 25 0.005058148 56.896767 5 CGGGAGA 705 0.0 56.49324 4 TTGTGTT 35015 0.0 48.74829 1 CGGACGG 40 5.4300914E-4 47.427654 1 AGAGCGG 385 0.0 46.792805 8 AAGCGGC 185 0.0 46.12896 9 ATCGGGA 935 0.0 44.12545 2 GAGCGGC 410 0.0 43.941277 9 AACCGGA 130 0.0 43.77432 2 CGAGTGG 110 3.45608E-11 43.116055 1 GAACCGG 160 0.0 41.4992 1 GAGAGCG 680 0.0 41.133842 7 TCGGGAA 185 0.0 41.006676 3 GGAGAGC 710 0.0 40.735943 6 CGGAGCG 85 3.6868732E-7 39.05807 1 CCCGTGT 280 0.0 38.187943 38-39 TGTGTTT 60070 0.0 38.12688 1 CAAGGCG 75 6.9984653E-6 37.942127 1 CGGTAGC 50 0.0016283372 37.942127 1 TCTCGGG 330 0.0 37.437134 48-49 >>END_MODULE