##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34310-1.34200000035810.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5724473 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.899934194815838 33.0 33.0 33.0 27.0 33.0 2 31.876735378086334 33.0 33.0 33.0 27.0 33.0 3 31.811652531158764 33.0 33.0 33.0 27.0 33.0 4 35.55369306484632 37.0 37.0 37.0 33.0 37.0 5 35.53505466791441 37.0 37.0 37.0 33.0 37.0 6 35.576382489706916 37.0 37.0 37.0 33.0 37.0 7 35.48243357947535 37.0 37.0 37.0 33.0 37.0 8 35.43283110951873 37.0 37.0 37.0 33.0 37.0 9 35.37250957424378 37.0 37.0 37.0 33.0 37.0 10-11 35.288256840411336 37.0 37.0 37.0 33.0 37.0 12-13 35.25632927258107 37.0 37.0 37.0 33.0 37.0 14-15 36.867365345246625 40.0 37.0 40.0 33.0 40.0 16-17 36.79495841800634 40.0 37.0 40.0 33.0 40.0 18-19 36.850125155625676 40.0 37.0 40.0 33.0 40.0 20-21 36.83017572097903 40.0 37.0 40.0 33.0 40.0 22-23 36.83895792678208 40.0 37.0 40.0 33.0 40.0 24-25 36.579968234630506 40.0 37.0 40.0 33.0 40.0 26-27 36.4665460034487 40.0 37.0 40.0 33.0 40.0 28-29 36.38809310481506 40.0 37.0 40.0 33.0 40.0 30-31 36.46392034690355 40.0 37.0 40.0 33.0 40.0 32-33 36.38744815461615 40.0 37.0 40.0 33.0 40.0 34-35 36.30720530955426 38.5 37.0 40.0 33.0 40.0 36-37 36.21042731793826 37.0 37.0 40.0 33.0 40.0 38-39 36.12475611292079 37.0 37.0 40.0 33.0 40.0 40-41 36.02342949298564 37.0 37.0 40.0 30.0 40.0 42-43 35.864881535819976 37.0 37.0 40.0 27.0 40.0 44-45 35.73856501725137 37.0 37.0 40.0 27.0 40.0 46-47 35.59544503048578 37.0 37.0 40.0 27.0 40.0 48-49 35.44397012615834 37.0 37.0 40.0 27.0 40.0 50-51 34.25095331919637 37.0 35.0 38.5 24.5 38.5 52-53 34.726026308447956 37.0 33.0 38.5 27.0 40.0 54-55 35.44860417718802 37.0 37.0 40.0 27.0 40.0 56-57 35.47009995505263 37.0 37.0 40.0 27.0 40.0 58-59 35.25253486216111 37.0 35.0 40.0 27.0 40.0 60-61 35.0335958436698 37.0 33.0 40.0 27.0 40.0 62-63 34.8106678990363 37.0 33.0 40.0 27.0 40.0 64-65 34.5284685594639 37.0 33.0 37.0 27.0 40.0 66-67 34.23522313757091 37.0 33.0 37.0 27.0 40.0 68-69 33.99521327116051 37.0 33.0 37.0 27.0 40.0 70-71 33.71320504088324 37.0 33.0 37.0 27.0 37.0 72-73 33.46164520297327 37.0 33.0 37.0 27.0 37.0 74-75 33.20959536362561 37.0 33.0 37.0 27.0 37.0 76-77 32.950431244937306 37.0 33.0 37.0 27.0 37.0 78-79 32.76772709033652 37.0 33.0 37.0 27.0 37.0 80-81 32.58785699574442 37.0 33.0 37.0 22.0 37.0 82-83 32.36069643441414 33.0 33.0 37.0 22.0 37.0 84-85 32.181406655250186 33.0 33.0 37.0 22.0 37.0 86-87 32.03246220219748 33.0 33.0 37.0 22.0 37.0 88-89 31.925778495243144 33.0 33.0 37.0 22.0 37.0 90-91 31.79197133081071 33.0 33.0 37.0 22.0 37.0 92-93 31.60232426635605 33.0 33.0 37.0 22.0 37.0 94-95 31.413226772141297 33.0 33.0 37.0 18.5 37.0 96-97 31.27635268783694 33.0 33.0 37.0 15.0 37.0 98-99 31.108081914265295 33.0 33.0 37.0 15.0 37.0 100-101 29.73519693428548 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.46242182023435774 2306 2 0.37548659858302 2306 3 0.4033827259556908 2306 4 0.5135200229368095 2306 5 0.5173041630746695 2306 6 0.4625544234547121 2306 7 0.5036402287112409 2306 8 0.467320976638149 2306 9 0.5419424331269056 2306 10-11 0.5107417359138609 2306 12-13 0.539375641974182 2306 14-15 0.8046729091977909 2306 16-17 0.765334772864783 2306 18-19 0.7455006001076399 2306 20-21 0.7069641473472146 2306 22-23 0.7095107845072093 2306 24-25 0.8997520428441987 2306 26-27 0.8588161531533132 2306 28-29 0.7970308971186881 2306 30-31 0.705047482829535 2306 32-33 0.7182455967942971 2306 34-35 0.7286597792681917 2306 36-37 0.6788616484617762 2306 38-39 0.7630443304953758 2306 40-41 0.5394018904716376 2306 42-43 0.5567652382979986 2306 44-45 0.7344400497229842 2306 46-47 0.5413880735621035 2306 48-49 0.7129521264180809 2306 50-51 0.6234148726899278 2306 52-53 0.6625548995946389 2306 54-55 0.7821112952109388 2306 56-57 0.6418047606756048 2306 58-59 0.8076762846314836 2306 60-61 0.8317347631130545 2306 62-63 0.7356664222334501 2306 64-65 0.7890769211577577 2306 66-67 0.6729196764328975 2306 68-69 0.7091855631662085 2306 70-71 0.6742996537717332 2306 72-73 0.6735241250720065 2306 74-75 0.7188334037782482 2306 76-77 0.6805208508836174 2306 78-79 0.6314853768762276 2306 80-81 0.5688837672200506 2306 82-83 0.5041933869862802 2306 84-85 0.6412783994771232 2306 86-87 0.6594003540726021 2306 88-89 0.6314226869774018 2306 90-91 0.617406435985199 2306 92-93 0.5212605574223907 2306 94-95 0.546032205474571 2306 96-97 0.5896237252927712 2306 98-99 0.7402410570484612 2306 100-101 0.7986035207256279 2307 1 0.40052356340620676 2307 2 0.3540686410824456 2307 3 0.3690122349338729 2307 4 0.48911716124881366 2307 5 0.5082663446027667 2307 6 0.44376659528034423 2307 7 0.47915913774400565 2307 8 0.4883512420223397 2307 9 0.5521030957471851 2307 10-11 0.495294501156323 2307 12-13 0.5032589108851937 2307 14-15 0.6833624610781328 2307 16-17 0.699019304863711 2307 18-19 0.7336833055036962 2307 20-21 0.7550497549682476 2307 22-23 0.6516611468713336 2307 24-25 0.8044414512703213 2307 26-27 0.7751429288411131 2307 28-29 0.6648016735452984 2307 30-31 0.7416859117742902 2307 32-33 0.6900970765773167 2307 34-35 0.6453595155847154 2307 36-37 0.695499593807348 2307 38-39 0.5624365194745522 2307 40-41 0.5483580253844238 2307 42-43 0.5544139912724901 2307 44-45 0.4440623120284002 2307 46-47 0.41565035621932367 2307 48-49 0.5588678773450013 2307 50-51 0.5338408240036827 2307 52-53 0.7014051852127849 2307 54-55 0.6463317831137516 2307 56-57 0.5903105916016074 2307 58-59 0.4899184007964763 2307 60-61 0.4597362529236406 2307 62-63 0.5335529515782724 2307 64-65 0.6666951382982589 2307 66-67 0.6282550250337451 2307 68-69 0.6479724729085135 2307 70-71 0.4893611673843381 2307 72-73 0.4624942437224604 2307 74-75 0.6232206643128393 2307 76-77 0.5716641992308737 2307 78-79 0.5318363113910038 2307 80-81 0.48381176275631077 2307 82-83 0.5182738444792321 2307 84-85 0.765123577840896 2307 86-87 0.6452089532381748 2307 88-89 0.634759013342638 2307 90-91 0.5480176748708026 2307 92-93 0.6116821238326473 2307 94-95 0.5452120172000683 2307 96-97 0.6752808238074088 2307 98-99 0.5467061888363496 2307 100-101 0.5634532158651098 2201 1 0.2838648922642655 2201 2 0.29746493709798116 2201 3 0.29020328961090414 2201 4 0.38357955489311735 2201 5 0.36525324356202304 2201 6 0.3245602382571988 2201 7 0.41049630118267544 2201 8 0.37483083738792544 2201 9 0.37100399974869447 2201 10-11 0.34241979185866 2201 12-13 0.3396035229805179 2201 14-15 0.5179087717659598 2201 16-17 0.44554125161074865 2201 18-19 0.4193289685632422 2201 20-21 0.4277138029391878 2201 22-23 0.41699378744363 2201 24-25 0.5789438612708722 2201 26-27 0.5205011440260492 2201 28-29 0.4915254653311365 2201 30-31 0.5319799903089688 2201 32-33 0.4144387737643882 2201 34-35 0.4789085528663648 2201 36-37 0.5541256214443848 2201 38-39 0.46414044233535634 2201 40-41 0.3586390713060368 2201 42-43 0.5010506419831557 2201 44-45 0.4592817518326129 2201 46-47 0.5067748995660466 2201 48-49 0.475813096288654 2201 50-51 0.4727924567045818 2201 52-53 0.5222849585272087 2201 54-55 0.651190240193273 2201 56-57 0.702054643327763 2201 58-59 0.6572601221453382 2201 60-61 0.5981687575074588 2201 62-63 0.4970006771696518 2201 64-65 0.5415950559083953 2201 66-67 0.48252593554417444 2201 68-69 0.629331003856727 2201 70-71 0.5321335358100114 2201 72-73 0.5048079991789152 2201 74-75 0.4938982624387691 2201 76-77 0.49466821838841213 2201 78-79 0.5502242660546273 2201 80-81 0.6141856637405354 2201 82-83 0.5881503961597403 2201 84-85 0.5482619845237124 2201 86-87 0.5958737991016037 2201 88-89 0.5350698520464405 2201 90-91 0.4978175838675263 2201 92-93 0.44063424161383224 2201 94-95 0.3348046659652404 2201 96-97 0.4041170756455976 2201 98-99 0.3346372993215887 2201 100-101 0.37833782116930337 1101 1 0.22790755738035884 1101 2 0.16231748418658398 1101 3 0.18506995833831397 1101 4 0.17984566103849886 1101 5 0.2161683931480809 1101 6 0.2809300831698067 1101 7 0.20903584915099316 1101 8 0.22102288220735034 1101 9 0.2400257581001881 1101 10-11 0.22613345704354515 1101 12-13 0.2194925660536029 1101 14-15 0.41680997961024957 1101 16-17 0.3770166885642183 1101 18-19 0.3434705659096764 1101 20-21 0.345318816441214 1101 22-23 0.2963041802847286 1101 24-25 0.3972112634546363 1101 26-27 0.38859896679850436 1101 28-29 0.3436343403536597 1101 30-31 0.3417116123369439 1101 32-33 0.30001195050375173 1101 34-35 0.3797940305807117 1101 36-37 0.3601518936519881 1101 38-39 0.36303791856931156 1101 40-41 0.23233901586099392 1101 42-43 0.1939684911386479 1101 44-45 0.21670315896717085 1101 46-47 0.22573671544559915 1101 48-49 0.3025380540504443 1101 50-51 0.21441703832562808 1101 52-53 0.31404508950116394 1101 54-55 0.45640987191675464 1101 56-57 0.5184429847745662 1101 58-59 0.5621143345346766 1101 60-61 0.5640173382284601 1101 62-63 0.5334470368027624 1101 64-65 0.6149994792033269 1101 66-67 0.4740966176741921 1101 68-69 0.41078375874590023 1101 70-71 0.5071003063769624 1101 72-73 0.4770166919930361 1101 74-75 0.4078839202450908 1101 76-77 0.5088274796186809 1101 78-79 0.5772380894430853 1101 80-81 0.5735644118855205 1101 82-83 0.45179301036998964 1101 84-85 0.594736406423479 1101 86-87 0.49592707201626496 1101 88-89 0.5480023324561785 1101 90-91 0.5271267931755013 1101 92-93 0.40611141571545417 1101 94-95 0.3941415309614271 1101 96-97 0.4506116036846102 1101 98-99 0.32527396495510175 1101 100-101 0.44137017785109833 2304 1 0.34843876051920475 2304 2 0.3645309814771629 2304 3 0.34578870194815536 2304 4 0.40658085504367847 2304 5 0.4211604906533637 2304 6 0.39564347395775457 2304 7 0.3699511966051503 2304 8 0.3739577750231078 2304 9 0.3943211154377977 2304 10-11 0.41912265554739747 2304 12-13 0.3183231938652753 2304 14-15 0.5083593691372101 2304 16-17 0.577760582768768 2304 18-19 0.5197189364195296 2304 20-21 0.5242770130961745 2304 22-23 0.546287006736577 2304 24-25 0.7094934123367906 2304 26-27 0.6234241800167624 2304 28-29 0.6452558161818231 2304 30-31 0.5554189260851601 2304 32-33 0.4916243895842527 2304 34-35 0.4471949352140072 2304 36-37 0.4241967674676843 2304 38-39 0.5255957494942649 2304 40-41 0.49354258604196133 2304 42-43 0.5324724381259074 2304 44-45 0.49694425960789346 2304 46-47 0.5356922878759107 2304 48-49 0.5463998019289207 2304 50-51 0.4477748850129686 2304 52-53 0.5404233399612508 2304 54-55 0.6308455677623002 2304 56-57 0.664958245535594 2304 58-59 0.736637974922175 2304 60-61 0.6338167884619068 2304 62-63 0.7067143988605622 2304 64-65 0.6841252204615955 2304 66-67 0.7332165438891707 2304 68-69 0.7780930444967069 2304 70-71 0.8641886417835423 2304 72-73 0.8272890458288344 2304 74-75 0.8380525991013386 2304 76-77 0.8772644684060893 2304 78-79 0.7645403406955538 2304 80-81 0.6400035978220657 2304 82-83 0.703123680782717 2304 84-85 0.690420227822834 2304 86-87 0.6885933811891292 2304 88-89 0.6451006152078662 2304 90-91 0.6710818906041034 2304 92-93 0.7829772085635902 2304 94-95 0.8081948184844343 2304 96-97 0.7847931682933655 2304 98-99 0.6866728875745558 2304 100-101 0.6448298090964144 2202 1 0.20321229497135462 2202 2 0.2139102714451404 2202 3 0.21064935039722243 2202 4 0.2561368405363993 2202 5 0.2759510928211739 2202 6 0.2389770310991466 2202 7 0.16459723757218114 2202 8 0.21108785226886084 2202 9 0.21570432073034596 2202 10-11 0.19403507888843308 2202 12-13 0.23975553819266082 2202 14-15 0.3191317683746817 2202 16-17 0.2524617943038763 2202 18-19 0.26304386096423826 2202 20-21 0.2903191377230385 2202 22-23 0.2710206046147974 2202 24-25 0.30383166079609225 2202 26-27 0.2613818910132224 2202 28-29 0.25025981181399004 2202 30-31 0.2878844107443328 2202 32-33 0.19127213638248008 2202 34-35 0.25477419053875394 2202 36-37 0.28607075374880964 2202 38-39 0.1544998836617495 2202 40-41 0.26549117469703987 2202 42-43 0.14390887024172372 2202 44-45 0.15209712991807578 2202 46-47 0.26901781143631354 2202 48-49 0.16384841917123794 2202 50-51 0.24582388995103344 2202 52-53 0.318396620663961 2202 54-55 0.35041411283686585 2202 56-57 0.3361163779682741 2202 58-59 0.3789332366233893 2202 60-61 0.39953707145310347 2202 62-63 0.30786271288997824 2202 64-65 0.3189174814421065 2202 66-67 0.36710915288181667 2202 68-69 0.34232948473731284 2202 70-71 0.4240155930136993 2202 72-73 0.4451499212832246 2202 74-75 0.32650043564847664 2202 76-77 0.33164702521947476 2202 78-79 0.28402628837035593 2202 80-81 0.2984715780351763 2202 82-83 0.4093638210031685 2202 84-85 0.354562236107121 2202 86-87 0.2997846353342766 2202 88-89 0.23272311720897676 2202 90-91 0.30986952515224786 2202 92-93 0.3800251096953353 2202 94-95 0.44468139112914074 2202 96-97 0.410360705103205 2202 98-99 0.46883321276202494 2202 100-101 0.41036133631749294 1102 1 0.1250193350337021 1102 2 0.0828976346575736 1102 3 0.1940323516807787 1102 4 0.09499333217567596 1102 5 0.08817823973863881 1102 6 0.10571599190033965 1102 7 0.1263216054146099 1102 8 0.11787075349200649 1102 9 0.09959896162411752 1102 10-11 0.10590059848294686 1102 12-13 0.19946107470541108 1102 14-15 -8.008915579793552E-4 1102 16-17 0.11742563255064198 1102 18-19 0.18352201656306733 1102 20-21 0.1529315172124157 1102 22-23 0.16685112068957153 1102 24-25 0.1894199269019481 1102 26-27 0.31763033062891566 1102 28-29 0.1909875701573327 1102 30-31 0.17509244142430447 1102 32-33 0.11364482126170117 1102 34-35 0.20104658786669205 1102 36-37 0.2578795267556089 1102 38-39 0.24974180564399973 1102 40-41 0.13819818078583523 1102 42-43 0.1174313197048491 1102 44-45 0.09966933348911056 1102 46-47 0.22682427580863873 1102 48-49 0.11010074567364114 1102 50-51 0.15935818609656138 1102 52-53 0.2923992230178243 1102 54-55 0.4040171393215459 1102 56-57 0.43724198902700806 1102 58-59 0.4122473411267933 1102 60-61 0.45973262982835905 1102 62-63 0.45506451671870707 1102 64-65 0.47497907635801795 1102 66-67 0.474447457905498 1102 68-69 0.38298244254060876 1102 70-71 0.40467990796705067 1102 72-73 0.4340662631451906 1102 74-75 0.38339729020744784 1102 76-77 0.39701511926451616 1102 78-79 0.3744854794867152 1102 80-81 0.39262266533543766 1102 82-83 0.31650983772755126 1102 84-85 0.3236568852250272 1102 86-87 0.4418845919617169 1102 88-89 0.39364449537955437 1102 90-91 0.2769069636057928 1102 92-93 0.32162744691849454 1102 94-95 0.4298534960104625 1102 96-97 0.3403487507237024 1102 98-99 0.41468754947998576 1102 100-101 0.3067820325695365 2305 1 0.07588213701958679 2305 2 0.12916536951240332 2305 3 0.09536638667617225 2305 4 0.13681483765626012 2305 5 0.09381028953818316 2305 6 0.13331419573778192 2305 7 0.12582437167112914 2305 8 0.17554740961066528 2305 9 0.1409991236526551 2305 10-11 0.13928491772071538 2305 12-13 0.13970960502344099 2305 14-15 0.05123634038518787 2305 16-17 0.08238493499699473 2305 18-19 0.13995342448031067 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-0.512073012939787 1213 84-85 -0.4434609692196929 1213 86-87 -0.5810130828473206 1213 88-89 -0.40371637090098034 1213 90-91 -0.38206642680804137 1213 92-93 -0.3264099911743408 1213 94-95 -0.3193650047869845 1213 96-97 -0.30685115694019416 1213 98-99 -0.4766183768190153 1213 100-101 -0.85044305766802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2727.0 3 216.0 4 243.0 5 343.0 6 778.0 7 4086.0 8 14409.0 9 22387.0 10 27400.0 11 25718.0 12 20557.0 13 16587.0 14 14005.0 15 13258.0 16 13295.0 17 14188.0 18 15661.0 19 17664.0 20 19686.0 21 22621.0 22 25360.0 23 28910.0 24 33327.0 25 38571.0 26 45887.0 27 54790.0 28 66331.0 29 82133.0 30 105406.0 31 139832.0 32 192358.0 33 275900.0 34 416889.0 35 692640.0 36 1267271.0 37 1696531.0 38 296403.0 39 105.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.11658949106448 23.80840642749142 24.85659826463821 15.218405816805891 2 32.04611445153355 21.703659184178733 33.43107021535883 12.819156148928892 3 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19.437606580973736 22-23 24.336084103665335 27.48245092945733 28.442546060106753 19.738918906770582 24-25 24.299018042891774 27.212771406419385 28.875888900972836 19.61232164971601 26-27 23.19379047142475 27.232233747565886 29.316589871924663 20.257385909084697 28-29 22.532845284865193 27.87192541960024 28.367190270947567 21.228039024586998 30-31 21.720182400030534 28.690397999275703 28.498468459170397 21.090951141523373 32-33 21.501037397814653 27.575428086420512 29.556808871143737 21.366725644621102 34-35 21.779012465920342 27.836616985631014 29.253108071842824 21.131262476605816 36-37 21.254257960710408 29.193777642860912 29.498487921987305 20.053476474441375 38-39 21.844240010883446 28.493489421706403 27.992363715331237 21.669906852078917 40-41 22.191143857266564 27.812254791437717 27.628999302926637 22.36760204836908 42-43 23.220143213259764 28.09124856255188 27.96953052578458 20.719077698403773 44-45 21.6706693572596 28.945177373196085 28.04384275465701 21.340310514887303 46-47 22.047772221967154 28.714563891452226 28.624702052277577 20.61296183430304 48-49 22.362942620003405 28.146126897543684 28.687501182843405 20.803429299609505 50-51 21.303747448107877 28.547178242545847 27.6981188283639 22.45095548098238 52-53 21.818220371692647 28.628040897213797 27.914800946909274 21.638937784184282 54-55 22.340709401126883 28.592633369910274 27.946915719260783 21.11974150970206 56-57 22.786923939443692 28.352585218526315 27.080096381792735 21.780394460237254 58-59 21.96005259221313 27.71517989054883 27.85723528864958 22.46753222858846 60-61 20.92412293725687 27.67727174382436 28.65841000049929 22.740195318419477 62-63 21.397813285678286 27.82314247401281 28.537381326255385 22.241662914053524 64-65 21.73032097412723 28.519885037947656 28.216090044555724 21.53370394336939 66-67 22.109458253781998 28.037319156223706 28.002699817836756 21.85052277215754 68-69 21.573624092636386 26.953513883220626 29.505795555550883 21.967066468592105 70-71 21.253656427123584 28.800175217756752 28.917375299655816 21.028793055463847 72-73 22.36454157852157 29.4774271063164 27.859357688130505 20.29867362703152 74-75 23.335382251719565 28.620786015768584 27.517505203811915 20.526326528699933 76-77 23.365619967208673 27.27692663494486 27.95496959012943 21.40248380771704 78-79 23.20014115784459 27.403124737299294 28.691303906184324 20.705430198671785 80-81 22.26781783872983 28.731789035549372 28.13007635957 20.870316766150797 82-83 21.464498718495285 28.702569124769163 28.53284662963471 21.300085527100844 84-85 21.652498360477633 27.933465838574623 28.933703717170673 21.480332083777075 86-87 20.811034897960294 28.481495167803555 29.477721188827033 21.22974874540912 88-89 20.763808046380944 29.15579262835483 29.269594931613916 20.810804393650308 90-91 21.54353222957984 28.12297495944006 28.62718651824676 21.706306292733338 92-93 21.436364693679018 28.60902741602854 27.731601350956065 22.223006539336385 94-95 21.133253152048322 29.728812416511275 27.234914673481637 21.903019757958766 96-97 21.01312717889865 30.597185808429682 26.499400571643882 21.890286441027783 98-99 21.35796931878983 29.1212247890473 27.379344901256452 22.141460990906413 100-101 22.027043727205662 29.07000544686209 27.134683408914128 21.76826741701812 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2320.0 1 1363.0 2 337.0 3 348.5 4 379.0 5 333.5 6 431.0 7 627.0 8 887.5 9 1218.0 10 1524.5 11 1825.5 12 1984.5 13 1950.5 14 1784.0 15 1583.0 16 1398.5 17 1212.5 18 1168.5 19 1185.0 20 1272.5 21 1425.0 22 1623.5 23 1966.5 24 2373.0 25 2909.5 26 3675.0 27 4604.5 28 5778.5 29 7204.0 30 9213.0 31 12335.5 32 18610.0 33 30230.0 34 49319.0 35 82399.5 36 126407.5 37 188536.0 38 270672.5 39 324672.0 40 343250.0 41 334627.0 42 345626.0 43 387922.5 44 385036.5 45 368863.0 46 384813.0 47 370330.0 48 307076.0 49 249040.0 50 201102.0 51 166599.0 52 149038.0 53 136918.5 54 111915.0 55 72294.5 56 47267.5 57 38482.5 58 33628.5 59 28476.5 60 22706.0 61 17673.0 62 12997.0 63 9305.5 64 6947.5 65 5244.5 66 4238.0 67 3599.0 68 2869.5 69 2099.0 70 1549.0 71 1182.5 72 933.0 73 695.0 74 500.0 75 353.0 76 255.5 77 207.0 78 167.5 79 126.0 80 100.5 81 83.5 82 68.5 83 59.0 84 54.5 85 49.0 86 34.5 87 28.5 88 23.0 89 17.5 90 17.0 91 13.5 92 12.5 93 11.5 94 6.0 95 3.0 96 3.5 97 3.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1674215250906066 2 0.15945572631751428 3 0.12610767838366954 4 0.11999357844818205 5 0.1329205325974985 6 0.1534115018098609 7 0.12895510206791086 8 0.11980142102163815 9 0.11295362909389214 10-11 0.10047213079701835 12-13 0.1294092923488328 14-15 0.12471016982698668 16-17 0.11479659350301764 18-19 0.11354757023048236 20-21 0.10672598158817413 22-23 0.11988003087795154 24-25 0.41249211936190455 26-27 0.297861479999993 28-29 0.3872932931992168 30-31 0.2225794409371832 32-33 0.2619455100932435 34-35 0.2758157825183209 36-37 0.2726451849803467 38-39 0.29214042934607254 40-41 0.28512668327722046 42-43 0.3403719434085897 44-45 0.28714433625593133 46-47 0.3133039495513386 48-49 0.3124305067034118 50-51 0.3130943232678362 52-53 0.31650948480322993 54-55 0.29469961689049806 56-57 0.30720731847280963 58-59 0.3064823609090304 60-61 0.28575556212772774 62-63 0.28349334515159735 64-65 0.2913193930690214 66-67 0.29138926849685554 68-69 0.28861172024044834 70-71 0.2621376675197874 72-73 0.25203193376927446 74-75 0.2612729591003399 76-77 0.25734246628466934 78-79 0.25422427531757075 80-81 0.26384088107324466 82-83 0.3058796853439609 84-85 0.31060501115124484 86-87 0.301058280823405 88-89 0.30826418431880104 90-91 0.28393880100404 92-93 0.2678150460313115 94-95 0.2532198160424549 96-97 0.2524162486223622 98-99 0.27372825411177587 100-101 0.2739378803952783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5724473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.385851018183914 #Duplication Level Percentage of deduplicated Percentage of total 1 84.65166931675093 28.261680202496166 2 6.257806642064076 4.17844400505106 3 2.5115245055059887 2.515481489080229 4 1.4846269328286028 1.9826213438799627 5 0.7798932970357707 1.3018700712458247 6 0.6242335386286422 1.2504340752725762 7 0.4230083864987506 0.9885746479762753 8 0.33891851962580216 0.9052066562824377 9 0.31203398818253064 0.9375768217864554 >10 2.036827328557082 13.743395605804324 >50 0.27023572980648164 6.335118579160784 >100 0.25939700910736374 17.60637642992478 >500 0.031742015683274144 7.342372284531251 >1k 0.017391960103195506 10.178960147030251 >5k 4.251331876925994E-4 0.9540206410863725 >10k+ 2.656964338204542E-4 1.5178669993913418 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 27481 0.4800616580775208 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23059 0.40281437260687575 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 13666 0.2387293991953495 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11046 0.19296099396398586 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 10305 0.18001657095771087 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8456 0.14771665444137827 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7379 0.12890269549703529 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7218 0.12609020952671102 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7166 0.12518182896486715 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6844 0.11955685702421866 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6323 0.11045558254882153 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.065968517975367E-4 0.0 0.0 2 5.764722796316796E-4 0.0 0.0 3 6.463477074658226E-4 0.0 0.0 4 6.987542783414299E-4 0.0 0.0 5 7.860985631341086E-4 0.0 0.0 6 9.083805618438588E-4 0.0 0.0 7 0.0010656002744706804 0.0 0.0 8 0.0011878822731804307 0.0 0.0 9 0.0014149774136413954 0.0 0.0 10-11 0.0025067809735498797 0.0 0.0 12-13 0.0032579418227669165 0.0 0.0 14-15 0.004419620810509544 0.0 0.0 16-17 0.005668644083044849 0.0 0.0 18-19 0.007057418211248442 0.0 0.0 20-21 0.008760631764705677 0.0 0.0 22-23 0.01059486174535193 0.0 0.0 24-25 0.012498967153832327 0.0 0.0 26-27 0.014778652986921242 0.0 0.0 28-29 0.017634811099641837 0.0 0.0 30-31 0.02837815812914132 0.0 0.0 32-33 0.032090290232830165 0.0 0.0 34-35 0.03551418619670317 0.0 8.734428479267873E-6 36-37 0.039610633153479805 0.0 1.7468856958535745E-5 38-39 0.04409139496334423 0.0 1.7468856958535745E-5 40-41 0.04902634705413057 0.0 1.7468856958535745E-5 42-43 0.05536754213007905 0.0 1.7468856958535745E-5 44-45 0.06218913077238726 0.0 1.7468856958535745E-5 46-47 0.06949984740953447 0.0 1.7468856958535745E-5 48-49 0.07826047917424014 0.0 1.7468856958535745E-5 50-51 0.08843608835258722 0.0 3.493771391707149E-5 52-53 0.09900474681250135 0.0 3.493771391707149E-5 54-55 0.11224614038707144 0.0 4.367214239633936E-5 56-57 0.12660554080698783 0.0 5.2406570875607236E-5 58-59 0.14265942035188217 0.0 5.2406570875607236E-5 60-61 0.161761615436041 0.0 5.2406570875607236E-5 62-63 0.18355401449181435 0.0 5.2406570875607236E-5 64-65 0.2084995422286034 0.0 5.2406570875607236E-5 66-67 0.2355762705143338 0.0 5.2406570875607236E-5 68-69 0.26557903234061897 0.0 5.2406570875607236E-5 70-71 0.29969570998063927 0.0 5.2406570875607236E-5 72-73 0.3366685457333802 0.0 5.2406570875607236E-5 74-75 0.3775980775872294 0.0 5.2406570875607236E-5 76-77 0.4238381419564735 0.0 5.2406570875607236E-5 78-79 0.4774675328191783 0.0 5.2406570875607236E-5 80-81 0.5376215417558962 0.0 5.2406570875607236E-5 82-83 0.6044748573362124 0.0 5.2406570875607236E-5 84-85 0.6799577882540454 0.0 5.2406570875607236E-5 86-87 0.7623234488135415 0.0 5.2406570875607236E-5 88-89 0.8517639964412445 0.0 5.2406570875607236E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1040 0.0 64.28644 9 AGAGCGG 1300 0.0 53.981895 8 CGGGAGA 1460 0.0 52.617924 4 TTGTGTT 70580 0.0 50.05105 1 CGGTTCG 70 4.357E-6 40.654476 1 TCGGGAG 2005 0.0 39.970898 3 CGAGTGG 215 0.0 37.502968 1 TCGGGAA 465 0.0 36.713104 3 ATTCGGA 260 0.0 36.48191 2 TGTGTTT 105625 0.0 33.5454 2 AACCGGA 340 0.0 33.47752 2 GATCCGG 510 0.0 32.550156 1 GATCCGA 205 0.0 32.391373 1 GGTCCGG 240 0.0 31.620148 1 ACGGCGA 120 1.527169E-7 31.620148 1 ACGACGC 220 0.0 31.254812 16-17 GAACCGG 305 0.0 31.101786 1 GCCGTAT 390 0.0 31.061396 58-59 AGGCCGC 185 9.094947E-12 30.765549 1 CGGAAAG 545 0.0 30.453915 5 >>END_MODULE