FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724473
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA274810.4800616580775208No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC230590.40281437260687575No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT136660.2387293991953495No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC110460.19296099396398586No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT103050.18001657095771087No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC84560.14771665444137827No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC73790.12890269549703529No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT72180.12609020952671102No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG71660.12518182896486715No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT68440.11955685702421866No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA63230.11045558254882153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10400.064.286449
AGAGCGG13000.053.9818958
CGGGAGA14600.052.6179244
TTGTGTT705800.050.051051
CGGTTCG704.357E-640.6544761
TCGGGAG20050.039.9708983
CGAGTGG2150.037.5029681
TCGGGAA4650.036.7131043
ATTCGGA2600.036.481912
TGTGTTT1056250.033.54542
AACCGGA3400.033.477522
GATCCGG5100.032.5501561
GATCCGA2050.032.3913731
GGTCCGG2400.031.6201481
ACGGCGA1201.527169E-731.6201481
ACGACGC2200.031.25481216-17
GAACCGG3050.031.1017861
GCCGTAT3900.031.06139658-59
AGGCCGC1859.094947E-1230.7655491
CGGAAAG5450.030.4539155
Produced by FastQC (version 0.11.2)