##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8266348 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.043699103884812 33.0 33.0 33.0 27.0 33.0 2 30.708506585979684 33.0 33.0 33.0 27.0 33.0 3 30.50378800892486 33.0 33.0 33.0 27.0 33.0 4 33.97312319781359 37.0 37.0 37.0 27.0 37.0 5 34.04689688844457 37.0 37.0 37.0 27.0 37.0 6 34.50776086368491 37.0 37.0 37.0 27.0 37.0 7 33.92075980832164 37.0 37.0 37.0 27.0 37.0 8 34.24825061804802 37.0 37.0 37.0 27.0 37.0 9 33.74344208591267 37.0 37.0 37.0 22.0 37.0 10-11 33.76112304974337 37.0 37.0 37.0 22.0 37.0 12-13 33.709893837036624 37.0 37.0 37.0 22.0 37.0 14-15 35.173334524508284 40.0 37.0 40.0 22.0 40.0 16-17 35.08996554463954 40.0 37.0 40.0 22.0 40.0 18-19 35.15163921238254 38.5 37.0 40.0 22.0 40.0 20-21 35.17896675775083 40.0 37.0 40.0 22.0 40.0 22-23 35.739675610075935 38.5 37.0 40.0 27.0 40.0 24-25 35.111780982363676 37.0 37.0 40.0 22.0 40.0 26-27 35.00402051788771 37.0 37.0 40.0 21.0 40.0 28-29 34.69981072657478 37.0 37.0 40.0 15.0 40.0 30-31 34.75215149422695 37.0 37.0 40.0 18.5 40.0 32-33 34.548355876137805 37.0 37.0 40.0 15.0 40.0 34-35 34.623950503898456 37.0 37.0 40.0 18.5 40.0 36-37 34.48966732346618 37.0 37.0 40.0 15.0 40.0 38-39 34.61502993825084 37.0 37.0 40.0 18.5 40.0 40-41 34.24965728517599 37.0 33.0 40.0 15.0 40.0 42-43 34.09282871952645 37.0 33.0 40.0 15.0 40.0 44-45 33.9837909679099 37.0 33.0 40.0 15.0 40.0 46-47 33.89401831377049 37.0 33.0 40.0 15.0 40.0 48-49 33.78340719505155 37.0 33.0 40.0 15.0 40.0 50-51 32.79396844894505 37.0 33.0 38.5 15.0 38.5 52-53 33.275494208567075 37.0 33.0 38.5 15.0 40.0 54-55 33.89774208634817 37.0 33.0 40.0 15.0 40.0 56-57 34.11091923543505 37.0 33.0 40.0 18.5 40.0 58-59 33.64881051463112 37.0 33.0 40.0 15.0 40.0 60-61 33.508036620282624 37.0 33.0 40.0 15.0 40.0 62-63 33.184865735146886 37.0 33.0 40.0 15.0 40.0 64-65 32.96630241068971 37.0 33.0 38.5 15.0 40.0 66-67 32.81335518417565 37.0 33.0 37.0 15.0 40.0 68-69 32.83632167433551 37.0 33.0 37.0 15.0 40.0 70-71 32.454274547841436 37.0 33.0 37.0 15.0 40.0 72-73 32.63388929428086 37.0 33.0 37.0 22.0 37.0 74-75 32.5884787937793 37.0 33.0 37.0 22.0 37.0 76-77 32.2401817586194 37.0 33.0 37.0 22.0 37.0 78-79 31.804760941591134 37.0 33.0 37.0 18.5 37.0 80-81 31.3553933974229 33.0 33.0 37.0 15.0 37.0 82-83 31.051987104825493 33.0 33.0 37.0 15.0 37.0 84-85 30.8412884383769 33.0 33.0 37.0 15.0 37.0 86-87 30.698226230011123 33.0 30.0 37.0 15.0 37.0 88-89 30.5597641183265 33.0 27.0 37.0 15.0 37.0 90-91 30.4134771485546 33.0 27.0 37.0 15.0 37.0 92-93 30.233675136831888 33.0 27.0 37.0 15.0 37.0 94-95 30.070264704558774 33.0 27.0 37.0 15.0 37.0 96-97 29.943630548822767 33.0 27.0 37.0 15.0 37.0 98-99 29.78310022757329 33.0 27.0 37.0 15.0 37.0 100-101 28.55143159954069 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.23093913007304323 2306 2 0.11802303697569627 2306 3 0.1679076030607014 2306 4 0.3136005752499855 2306 5 0.2366180549512933 2306 6 0.049157389361084824 2306 7 0.5056964344484385 2306 8 0.05468725148389808 2306 9 0.3207031410860921 2306 10-11 0.3044135165498574 2306 12-13 0.2866420881361549 2306 14-15 0.6188581527638846 2306 16-17 0.5607705679847328 2306 18-19 0.5352606055318034 2306 20-21 0.5149525219678637 2306 22-23 0.5085040414951649 2306 24-25 0.7209425635205946 2306 26-27 0.5788711171441321 2306 28-29 0.5603395533993023 2306 30-31 0.7027485813726955 2306 32-33 0.7052473557846852 2306 34-35 0.5606074907553946 2306 36-37 0.490768453922918 2306 38-39 0.42459749029870153 2306 40-41 0.48434955293219417 2306 42-43 0.4642183461106768 2306 44-45 0.5924099248421086 2306 46-47 0.3780691261151219 2306 48-49 0.5656948791479195 2306 50-51 0.5723953336985872 2306 52-53 0.5361622222813196 2306 54-55 0.7541463253380627 2306 56-57 0.7012275306922504 2306 58-59 0.8388352689420486 2306 60-61 0.6741234114599877 2306 62-63 0.7043614295346856 2306 64-65 0.7353163326625491 2306 66-67 0.5792071446333296 2306 68-69 0.6122815758947624 2306 70-71 0.6124230751835142 2306 72-73 0.3981261645338705 2306 74-75 0.606211872323442 2306 76-77 0.6429644688352667 2306 78-79 0.6057363064996188 2306 80-81 0.5789369817931842 2306 82-83 0.5488461184411229 2306 84-85 0.5834384819628582 2306 86-87 0.5751640207800861 2306 88-89 0.6048707009606709 2306 90-91 0.6067080455420175 2306 92-93 0.44017183848754726 2306 94-95 0.44110852302031134 2306 96-97 0.4975467729387191 2306 98-99 0.5667200390542071 2306 100-101 0.43861032442709913 2307 1 0.1816124826944474 2307 2 0.08538554459794412 2307 3 0.1624345047304594 2307 4 0.2583882551026022 2307 5 0.16502860543976539 2307 6 0.20395551331505146 2307 7 0.4497287008833055 2307 8 0.1139328759892635 2307 9 0.33433236900103225 2307 10-11 0.230251639465898 2307 12-13 0.21687289628903983 2307 14-15 0.5657451942937897 2307 16-17 0.44402416503703535 2307 18-19 0.4856146346196013 2307 20-21 0.4393925300117729 2307 22-23 0.34814686620293145 2307 24-25 0.579093179138745 2307 26-27 0.4314785746217211 2307 28-29 0.4546896837883949 2307 30-31 0.5734283885784848 2307 32-33 0.464712079372795 2307 34-35 0.32987458729522245 2307 36-37 0.43246587987359675 2307 38-39 0.331421348258921 2307 40-41 0.3997854157706584 2307 42-43 0.38726056363341144 2307 44-45 0.3420294611499557 2307 46-47 0.20416040496613164 2307 48-49 0.31092805506291654 2307 50-51 0.28837626834835106 2307 52-53 0.22435990122575333 2307 54-55 0.3733748241120125 2307 56-57 0.3405674115905697 2307 58-59 0.3326190825310533 2307 60-61 0.28136860133505337 2307 62-63 0.362712656230066 2307 64-65 0.3796551107675654 2307 66-67 0.39967498847779837 2307 68-69 0.32788756081892956 2307 70-71 0.24971695013973516 2307 72-73 0.1398329966627827 2307 74-75 0.3324294858787198 2307 76-77 0.35428188518515213 2307 78-79 0.39118251613414756 2307 80-81 0.36733640704325765 2307 82-83 0.4246610502625465 2307 84-85 0.35927579400146925 2307 86-87 0.28261614657636613 2307 88-89 0.2872180613924442 2307 90-91 0.35964261097658223 2307 92-93 0.5141395915137394 2307 94-95 0.3174367402576195 2307 96-97 0.3665960677996978 2307 98-99 0.38663502666399907 2307 100-101 0.38540362852559795 2201 1 0.21573553933753686 2201 2 0.13605606777468537 2201 3 0.24524480486598677 2201 4 0.2904442989022229 2201 5 0.2907345058762516 2201 6 0.20039356453342805 2201 7 0.39176621573098913 2201 8 0.21535406693794812 2201 9 0.31537159320135544 2201 10-11 0.34111688410428087 2201 12-13 0.32765037063379765 2201 14-15 0.6130139083558177 2201 16-17 0.43548353232536385 2201 18-19 0.4924873327053376 2201 20-21 0.47164609435292704 2201 22-23 0.42716569898661305 2201 24-25 0.6514067801550283 2201 26-27 0.5187895320625486 2201 28-29 0.4458377431528149 2201 30-31 0.5380031317868301 2201 32-33 0.3385664303271696 2201 34-35 0.39881733056098057 2201 36-37 0.4537656517605413 2201 38-39 0.3263042055692438 2201 40-41 0.4400084330591625 2201 42-43 0.38031714093286695 2201 44-45 0.39341051999164023 2201 46-47 0.39411894509046874 2201 48-49 0.4117699919783533 2201 50-51 0.38244778125103807 2201 52-53 0.44298598362635744 2201 54-55 0.5990032414715714 2201 56-57 0.5843414026359071 2201 58-59 0.595412911157986 2201 60-61 0.5587877719222263 2201 62-63 0.5268277043945773 2201 64-65 0.5963018010948318 2201 66-67 0.539532989001728 2201 68-69 0.5175839862503651 2201 70-71 0.5573370264804467 2201 72-73 0.43721818953015656 2201 74-75 0.42333110061288437 2201 76-77 0.4859150466262676 2201 78-79 0.5470353443412144 2201 80-81 0.5588649439019946 2201 82-83 0.4509347213275987 2201 84-85 0.43454756791236804 2201 86-87 0.5227766032330905 2201 88-89 0.5359554849709838 2201 90-91 0.49156210991735705 2201 92-93 0.45977643601767326 2201 94-95 0.36527035409703856 2201 96-97 0.5261158102843488 2201 98-99 0.48429988828299386 2201 100-101 0.4190020072124021 1101 1 0.22277389302543327 1101 2 0.07388850525240187 1101 3 0.20105616181901453 1101 4 0.22797974347293604 1101 5 0.26999859845841456 1101 6 0.21422468544875528 1101 7 0.3869365974962591 1101 8 0.22582047917007486 1101 9 0.30575097377208493 1101 10-11 0.2851510639669357 1101 12-13 0.2593229423236565 1101 14-15 0.5451875311656664 1101 16-17 0.5351227864670847 1101 18-19 0.4804412546141208 1101 20-21 0.42293599804720117 1101 22-23 0.3957105521811499 1101 24-25 0.5318613375529821 1101 26-27 0.4749090788736581 1101 28-29 0.5127930970769867 1101 30-31 0.5337330376388749 1101 32-33 0.52636490371993 1101 34-35 0.3969433839452563 1101 36-37 0.3246051135584409 1101 38-39 0.3691673037825325 1101 40-41 0.39010815061731563 1101 42-43 0.32626908481315553 1101 44-45 0.26638051275990193 1101 46-47 0.3415779737047018 1101 48-49 0.35838447189540545 1101 50-51 0.28025574667245934 1101 52-53 0.3607858812712976 1101 54-55 0.5569895688497226 1101 56-57 0.6240338228872417 1101 58-59 0.6201804674298756 1101 60-61 0.5658832300606562 1101 62-63 0.6581695579607469 1101 64-65 0.5964017276627374 1101 66-67 0.4812822692617118 1101 68-69 0.3921028159390758 1101 70-71 0.4985955319333115 1101 72-73 0.5017950373405995 1101 74-75 0.5188924998157418 1101 76-77 0.5611338997439717 1101 78-79 0.57528485381523 1101 80-81 0.5044648307580495 1101 82-83 0.45562460161278295 1101 84-85 0.5391572730513623 1101 86-87 0.4609874478932632 1101 88-89 0.593760018651313 1101 90-91 0.4881133992189035 1101 92-93 0.41014274054010613 1101 94-95 0.47196119921105506 1101 96-97 0.4053799824892792 1101 98-99 0.34350534467171556 1101 100-101 0.5245526045921345 2304 1 0.3569752105569748 2304 2 0.3082113762437899 2304 3 0.2628323102553445 2304 4 0.3559340086877114 2304 5 0.3113552809155138 2304 6 0.3345181942004203 2304 7 0.33962431638245505 2304 8 0.3231522220961054 2304 9 0.3354088341660173 2304 10-11 0.3970756856690798 2304 12-13 0.4313640839317756 2304 14-15 0.46030083584766857 2304 16-17 0.4667323209556926 2304 18-19 0.45427257515726893 2304 20-21 0.4075390765176081 2304 22-23 0.5465730277236673 2304 24-25 0.6599065902913708 2304 26-27 0.5737834900472976 2304 28-29 0.5534296688053715 2304 30-31 0.4933101670263156 2304 32-33 0.45713450238042697 2304 34-35 0.5313634219407746 2304 36-37 0.42865939733533054 2304 38-39 0.47237570901249626 2304 40-41 0.44708980347919436 2304 42-43 0.4129119837613615 2304 44-45 0.45878235609183804 2304 46-47 0.4980871449061368 2304 48-49 0.45280447200904916 2304 50-51 0.42401219842895443 2304 52-53 0.4512701205180605 2304 54-55 0.7107033419410911 2304 56-57 0.7430659836503324 2304 58-59 0.6528025133540751 2304 60-61 0.6433472091347099 2304 62-63 0.6943073731616067 2304 64-65 0.7126952420966717 2304 66-67 0.6767928411454065 2304 68-69 0.7095889464383376 2304 70-71 0.7563492410421944 2304 72-73 0.7254519828873001 2304 74-75 0.7302669583417156 2304 76-77 0.7570883051216839 2304 78-79 0.690155951272029 2304 80-81 0.650063477996369 2304 82-83 0.6681117454521477 2304 84-85 0.611372434513374 2304 86-87 0.624671185394611 2304 88-89 0.687922848199932 2304 90-91 0.6838627926354128 2304 92-93 0.6971546562832458 2304 94-95 0.6784690918992844 2304 96-97 0.6923706607073896 2304 98-99 0.723725916612235 2304 100-101 0.6075102128822039 2202 1 0.18684869850157781 2202 2 0.13876223707756807 2202 3 0.14301451435881063 2202 4 0.17572903859240796 2202 5 0.16329335233979236 2202 6 0.1766590606791425 2202 7 0.1386131762802023 2202 8 0.10602032660301575 2202 9 0.13791400306175206 2202 10-11 0.1362080876843308 2202 12-13 0.24520295064239406 2202 14-15 0.3100857226143674 2202 16-17 0.3091182180584866 2202 18-19 0.19029989477063936 2202 20-21 0.266001075083949 2202 22-23 0.2536535050816653 2202 24-25 0.39544594329711913 2202 26-27 0.2914248380817668 2202 28-29 0.31494575902978994 2202 30-31 0.34714396216792665 2202 32-33 0.3713314045128939 2202 34-35 0.3739164882829016 2202 36-37 0.3290985418926127 2202 38-39 0.3468601100764914 2202 40-41 0.2996349503875493 2202 42-43 0.2719936891288057 2202 44-45 0.2144100415690815 2202 46-47 0.293098652734173 2202 48-49 0.29372460738629513 2202 50-51 0.34771885312301976 2202 52-53 0.3834590642860576 2202 54-55 0.5027352553761588 2202 56-57 0.47410230387050945 2202 58-59 0.3847766189425741 2202 60-61 0.46971471317800706 2202 62-63 0.40872104942920373 2202 64-65 0.4222354833146369 2202 66-67 0.44755185000383335 2202 68-69 0.4049613354371999 2202 70-71 0.515488750024609 2202 72-73 0.5798651577106497 2202 74-75 0.4906274946373941 2202 76-77 0.5310414714987672 2202 78-79 0.5047392193682434 2202 80-81 0.4750018236281335 2202 82-83 0.4885055674897991 2202 84-85 0.5276346310338162 2202 86-87 0.5517686187617699 2202 88-89 0.4070692800750315 2202 90-91 0.39045474269918046 2202 92-93 0.47299029090008204 2202 94-95 0.400999542471272 2202 96-97 0.4954373307875457 2202 98-99 0.48354238590311027 2202 100-101 0.4026105313521917 1102 1 0.09319031581600257 1102 2 0.011185450430616584 1102 3 0.03926138638889043 1102 4 -0.004534854219997442 1102 5 -0.0635468623982689 1102 6 0.15361623033729188 1102 7 0.0763066742999925 1102 8 0.23482288367620185 1102 9 0.11444687450075719 1102 10-11 0.12298721889869313 1102 12-13 0.07726535883255536 1102 14-15 -0.03487278266266003 1102 16-17 0.10293893435328982 1102 18-19 0.13084931755104634 1102 20-21 0.10698332023723367 1102 22-23 0.08559769738224787 1102 24-25 0.07757436864417144 1102 26-27 0.1994957849808614 1102 28-29 0.11059473697258682 1102 30-31 0.23736572382805576 1102 32-33 0.15905522524612792 1102 34-35 0.19497595592824268 1102 36-37 0.18548523166072073 1102 38-39 0.13092340843905959 1102 40-41 0.1945891305392351 1102 42-43 0.18107018713984502 1102 44-45 0.1934559081092928 1102 46-47 0.2453417301107521 1102 48-49 0.17249020904654344 1102 50-51 0.1494887841238537 1102 52-53 0.23543157724080999 1102 54-55 0.44375064241038586 1102 56-57 0.5843611247033493 1102 58-59 0.4529074399154993 1102 60-61 0.4323903045303581 1102 62-63 0.4078583211257225 1102 64-65 0.4791318407376579 1102 66-67 0.5002047028338197 1102 68-69 0.3814705907190685 1102 70-71 0.374983549444174 1102 72-73 0.5108738601654821 1102 74-75 0.46806651676492095 1102 76-77 0.5068607873248254 1102 78-79 0.3399700115514257 1102 80-81 0.3727872585730765 1102 82-83 0.23666618228952174 1102 84-85 0.3061565089084901 1102 86-87 0.3484353792205468 1102 88-89 0.3533396223500631 1102 90-91 0.3584436271450535 1102 92-93 0.3780097546846939 1102 94-95 0.4187492552314289 1102 96-97 0.390516851306419 1102 98-99 0.385176650998023 1102 100-101 0.37942474309801 2305 1 0.22361712645519916 2305 2 0.22509590503798194 2305 3 0.11137414144478797 2305 4 0.1716041517866671 2305 5 0.18924047560226143 2305 6 0.3225100826928582 2305 7 0.1726550788067911 2305 8 0.275618553752885 2305 9 0.14214480550093356 2305 10-11 0.18201528347600515 2305 12-13 0.18069286876090018 2305 14-15 0.02021142353938643 2305 16-17 0.15869785912825307 2305 18-19 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-0.3330955416901915 1213 54-55 -0.5246126136038924 1213 56-57 -0.8404666993594674 1213 58-59 -1.1041614591811566 1213 60-61 -0.7780890077900793 1213 62-63 -0.786801845376182 1213 64-65 -0.6413289334541901 1213 66-67 -0.5453208610740035 1213 68-69 -0.4830168071444234 1213 70-71 -0.5115385315153347 1213 72-73 -0.47325057310155216 1213 74-75 -0.5666419323022822 1213 76-77 -0.795046344582822 1213 78-79 -0.762074636012926 1213 80-81 -0.777305465975985 1213 82-83 -0.6388561773784787 1213 84-85 -0.5821601804100318 1213 86-87 -0.7566958429092878 1213 88-89 -0.5853451881422735 1213 90-91 -0.5583577653955167 1213 92-93 -0.5055489130366659 1213 94-95 -0.4788767810633239 1213 96-97 -0.5205904640181451 1213 98-99 -0.8049392830710254 1213 100-101 -1.1024922690594856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3985.0 3 480.0 4 671.0 5 711.0 6 1543.0 7 9858.0 8 44172.0 9 125972.0 10 170346.0 11 129254.0 12 84255.0 13 57648.0 14 42790.0 15 34090.0 16 30359.0 17 29327.0 18 29712.0 19 31359.0 20 33122.0 21 35737.0 22 39227.0 23 42671.0 24 48095.0 25 55115.0 26 63827.0 27 76225.0 28 93271.0 29 113915.0 30 140267.0 31 182856.0 32 248473.0 33 351737.0 34 528896.0 35 878314.0 36 1632588.0 37 2335889.0 38 539357.0 39 234.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.96717460785992 23.6208225475046 25.583979695438853 13.828023149196621 2 29.08209938097413 25.61354320519453 33.45422249793719 11.850134915894147 3 36.473596279800354 20.00410373137953 28.593608377813638 14.928691611006478 4 26.67263621116975 18.052304691914216 36.66082348949954 18.614235607416497 5 34.24169879839003 17.039744330367455 32.907713927262364 15.810842943980147 6 27.61014880035048 17.52823930570175 40.14441203894863 14.717199854999139 7 33.588836297054655 19.522768269632827 31.05859383895464 15.829801594357876 8 26.550406492347058 30.621470678326197 27.34730663014374 15.480816199183007 9 33.19045647847146 26.295610727099962 23.83960797355551 16.674324820873068 10-11 26.577459669487148 29.371634204447826 27.255778937342402 16.79512718872262 12-13 28.36964063994145 24.066805549137403 27.05031571364693 20.51323809727422 14-15 25.25430878247617 24.74635437500738 28.760819081251327 21.238517761265122 16-17 25.927779174274306 26.216754038366197 29.89583037490371 17.959636412455783 18-19 25.347399430441513 26.588715504397403 30.089481286125753 17.974403779035335 20-21 24.434358755039153 28.721897623659174 28.80841328473165 18.03533033657002 22-23 27.20079884313329 26.63531295784339 28.135336061022432 18.02855213800089 24-25 23.80225267326617 29.699596798561018 28.363651536343426 18.134498991829382 26-27 22.499405765151835 30.81988878253261 28.305283466706044 18.375421985609506 28-29 23.145486880375483 29.684025976636136 28.039820252549212 19.130666890439173 30-31 25.13166255444087 27.47444917775084 28.303518029274134 19.090370238534152 32-33 22.42902307488779 26.987869069125086 29.94574049909911 20.63736735688801 34-35 20.173129758343606 27.20396216609071 33.55149329359636 19.071414781969327 36-37 21.443406262167777 27.867404728951943 29.790699056659847 20.898489952220434 38-39 20.45557711260892 27.47986237375369 27.757461250837657 24.30709926279973 40-41 22.231253361529898 27.002513440205544 28.85546666420804 21.910766534056517 42-43 23.836300992834282 27.14519025530631 30.34444837583381 18.674060376025594 44-45 21.58034244192225 29.28641999991265 30.635135672861402 18.498101885303697 46-47 23.184427249280056 29.735939802106685 28.658034729469822 18.42159821914343 48-49 20.901115685703818 28.85139943523732 30.237368150799316 20.010116728259547 50-51 20.138119550752574 27.906560068886854 30.512048070326525 21.443272310034047 52-53 24.403008865172275 27.50546723849635 27.95040051822419 20.14112337810719 54-55 25.443949623550033 27.46087118781927 27.89185107392823 19.203328114702465 56-57 25.682236254562397 27.102039974372914 27.351788799798086 19.863934971266602 58-59 24.047076767760114 26.773942450268283 27.883735660496868 21.295245121474732 60-61 20.87330259351101 27.06392722065919 27.846392116707726 24.21637806912208 62-63 23.380128770853993 27.213327146509446 28.257917769831952 21.148626312804602 64-65 21.085489093438518 28.64178772565524 30.11545982963885 20.157263351267385 66-67 20.420993145694958 28.598437353845412 28.531310126214475 22.449259374245152 68-69 20.176993265441006 27.18301763369395 32.61136067401881 20.02862842684623 70-71 19.777906960243726 31.47059925799996 29.655386838080183 19.096106943676137 72-73 19.733841696755697 34.35844423717412 27.016906895832026 18.89080717023816 74-75 20.535358227491923 33.77559720753326 26.673193823195522 19.0158507417793 76-77 20.73148206205232 32.7836306729682 27.25003417058026 19.234853094399217 78-79 20.696143658951883 32.5102781591241 27.72355450880708 19.070023673116946 80-81 19.976324679368904 32.99178974568739 27.491134641560556 19.54075093338314 82-83 19.287710599586394 32.38022313941636 28.00003567337963 20.33203058761761 84-85 19.422077656930757 31.584477836084822 28.35128928983504 20.64215521714938 86-87 19.372446024895748 31.437103189360492 28.99538802707027 20.19506275867349 88-89 19.646455211938978 31.669236151612694 28.602374717360195 20.081933919088133 90-91 20.14965243334267 30.921283045703184 28.2222839838135 20.70678053714064 92-93 19.813803706095864 31.646419896840634 27.866553864534342 20.673222532529163 94-95 19.510547252115153 32.22831207886652 27.749505392430713 20.511635276587608 96-97 20.03809966404479 32.44632199261146 27.245025371882846 20.270552971460905 98-99 20.46863836564576 31.338259750436375 27.145975431799407 21.04712645211845 100-101 20.53449060867157 31.424916285695897 26.74142158475631 21.29917152087622 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3278.0 1 1925.0 2 496.5 3 588.5 4 710.0 5 665.5 6 805.5 7 1140.0 8 1552.5 9 2067.5 10 2556.5 11 2881.5 12 3038.0 13 2994.5 14 2760.0 15 2580.0 16 2487.5 17 2445.5 18 2598.5 19 2914.0 20 3431.0 21 4230.0 22 5389.0 23 7056.5 24 9217.0 25 11943.0 26 15856.5 27 20947.5 28 26541.5 29 32727.5 30 40119.5 31 48737.5 32 60328.0 33 84045.5 34 124633.5 35 200069.0 36 300746.0 37 403897.0 38 521712.5 39 584318.0 40 580034.5 41 530410.5 42 476328.5 43 479615.0 44 471419.5 45 446917.0 46 449820.5 47 409628.0 48 325579.5 49 275136.5 50 236414.5 51 201856.0 52 179465.5 53 155314.5 54 122732.5 55 89870.0 56 67990.0 57 52622.5 58 41593.5 59 32352.5 60 24555.5 61 18528.0 62 13629.0 63 10174.0 64 7648.5 65 5617.0 66 4116.0 67 3034.5 68 2248.5 69 1602.0 70 1123.0 71 832.5 72 652.5 73 494.5 74 389.0 75 314.5 76 244.0 77 208.5 78 177.0 79 149.0 80 121.0 81 96.0 82 84.0 83 68.0 84 52.5 85 48.5 86 49.0 87 44.0 88 31.5 89 24.0 90 26.0 91 20.5 92 12.0 93 9.0 94 11.0 95 9.5 96 4.5 97 3.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1647765131591363 2 0.15796576674487936 3 0.1263798717402171 4 0.12091191902397529 5 0.13315432643290606 6 0.15247361954759225 7 0.12987597425126549 8 0.12074255765665805 9 0.11315758784895096 10-11 0.10117527111125735 12-13 0.12927716084539387 14-15 0.12569637765068686 16-17 0.11582502938419723 18-19 0.11402858916658239 20-21 0.10746583618303997 22-23 0.11986550771876528 24-25 0.4096428071985355 26-27 0.29828165956719943 28-29 0.384625713797677 30-31 0.21991573546141538 32-33 0.25927410750188595 34-35 0.27186733488597387 36-37 0.26901843474288767 38-39 0.2888760550608322 40-41 0.28142415489887435 42-43 0.33646055065671077 44-45 0.28346253992694237 46-47 0.30962282255719215 48-49 0.30898166880949124 50-51 0.30955023911405616 52-53 0.31330643229634175 54-55 0.2913499407477159 56-57 0.30325362542201223 58-59 0.30397945985337177 60-61 0.2817991693550767 62-63 0.27851476855317486 64-65 0.28720058724844394 66-67 0.28780544927457685 68-69 0.2841883743583019 70-71 0.2580946265509267 72-73 0.2484894175759356 74-75 0.25776800105681497 76-77 0.2534492861902257 78-79 0.2505096567432196 80-81 0.2597942888443603 82-83 0.30132411555864813 84-85 0.3072275689337057 86-87 0.2968541851855257 88-89 0.3033080630043642 90-91 0.280426132555755 92-93 0.26399807992598423 94-95 0.25004391298309725 96-97 0.24927573820990842 98-99 0.27047010360560675 100-101 0.27081487496050255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8266348.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.260053776397896 #Duplication Level Percentage of deduplicated Percentage of total 1 91.76422869637521 39.69725468154867 2 3.6609018116073817 3.167416184804957 3 1.2292020051209853 1.5952603453076986 4 0.7194995402210991 1.2450235520823325 5 0.467637787793475 1.0115017923910739 6 0.2667899098087625 0.6924807507196447 7 0.19712809723562483 0.5969440461076486 8 0.1356039789180371 0.469298833623025 9 0.10416024252616109 0.40553799237399385 >10 1.133743243915414 9.812943067097018 >50 0.13714757834161964 4.185773829879742 >100 0.14050095778406302 13.32486683308649 >500 0.024430839193450675 7.310425017358105 >1k 0.018427692203209146 14.199857083147528 >5k 3.985801204697738E-4 1.0964018662080453 >10k+ 1.9903883488955732E-4 1.1890141242642154 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16777 0.20295540424864764 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 16649 0.20140695746174733 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 15810 0.19125737266323653 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 12760 0.154360789069127 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12656 0.1531026760547705 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11444 0.13844082054130796 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10567 0.12783154060293617 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 9731 0.11771824752599333 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9315 0.11268579546856725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002274281218259865 0.0 0.0 2 0.0024920315476677246 0.0 0.0 3 0.0027702680796888785 0.0 0.0 4 0.0029275322064834437 0.0 0.0 5 0.003193671497981938 0.0 1.2097240522658857E-5 6 0.0035081997515710684 0.0 1.2097240522658857E-5 7 0.003979992131954764 0.0 1.2097240522658857E-5 8 0.004609048639133024 0.0 1.2097240522658857E-5 9 0.005165521703175332 0.0 1.2097240522658857E-5 10-11 0.008093053909658775 0.0 2.4194481045317714E-5 12-13 0.010948002673006265 0.0 2.4194481045317714E-5 14-15 0.013851340398444392 0.0 2.4194481045317714E-5 16-17 0.016325226085328128 0.0 2.4194481045317714E-5 18-19 0.01927090415259556 0.0 2.4194481045317714E-5 20-21 0.02263393701789472 0.0 2.4194481045317714E-5 22-23 0.026771193276644054 0.0 2.4194481045317714E-5 24-25 0.03162823534649158 0.0 2.4194481045317714E-5 26-27 0.03711433392351737 0.0 2.4194481045317714E-5 28-29 0.0434290934763453 0.0 3.6291721567976575E-5 30-31 0.06694008043213279 0.0 3.6291721567976575E-5 32-33 0.07386575063135498 0.0 3.6291721567976575E-5 34-35 0.08007163501947898 0.0 3.6291721567976575E-5 36-37 0.0865315614585788 0.0 3.6291721567976575E-5 38-39 0.0936023985440729 0.0 4.838896209063543E-5 40-41 0.10132648661779059 0.0 4.838896209063543E-5 42-43 0.10958285327450526 0.0 4.838896209063543E-5 44-45 0.11825657472925166 0.0 4.838896209063543E-5 46-47 0.1274928178683017 0.0 4.838896209063543E-5 48-49 0.13746094405897258 0.0 4.838896209063543E-5 50-51 0.14830612018753625 0.0 4.838896209063543E-5 52-53 0.1596170400762223 0.0 4.838896209063543E-5 54-55 0.1718050099028011 0.0 4.838896209063543E-5 56-57 0.18505753689537385 0.0 4.838896209063543E-5 58-59 0.1989754121166929 0.0 4.838896209063543E-5 60-61 0.21407276828897115 0.0 6.653482287462372E-5 62-63 0.23117221776774943 0.0 7.258344313595315E-5 64-65 0.25006810746414254 0.0 1.0282654444260028E-4 66-67 0.2696414426298046 0.0 1.0887516470392971E-4 68-69 0.29159793417843044 0.0 1.0887516470392971E-4 70-71 0.31582265832505474 0.0 1.1492378496525914E-4 72-73 0.3424970736775176 0.0 1.2097240522658858E-4 74-75 0.3713671381848429 0.0 1.3306964574924743E-4 76-77 0.4035578952156381 0.0 1.3306964574924743E-4 78-79 0.4403153605437371 0.0 1.451668862719063E-4 80-81 0.4804540045979192 0.0 1.451668862719063E-4 82-83 0.527215887838257 0.0 1.451668862719063E-4 84-85 0.5787440838445224 0.0 1.451668862719063E-4 86-87 0.6365749421630931 0.0 1.5726412679456514E-4 88-89 0.7007326572750143 0.0 1.5726412679456514E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5920 0.0 87.70173 9 CGGGAGA 8505 0.0 85.082085 4 AGAGCGG 6490 0.0 84.08938 8 GAGAGCG 8130 0.0 77.68268 7 ATTCGGA 1065 0.0 74.36425 2 TCGGGAG 9460 0.0 73.9866 3 CGGAAAG 3030 0.0 73.86815 5 AAGCGGC 2450 0.0 73.34811 9 TCGGGAA 3115 0.0 71.852486 3 GAACCGG 1080 0.0 68.06704 1 AAGCGTC 1440 0.0 66.51272 9 AACCGGA 1095 0.0 65.83045 2 AGAGCGT 20450 0.0 65.29048 8 GATCGGG 13760 0.0 64.35092 1 GAGCGTC 20450 0.0 64.108795 9 TCGGAGA 1885 0.0 63.896965 4 ATCGGGA 13530 0.0 63.68754 2 CGGAGAG 3465 0.0 63.499836 5 GATCCGG 1675 0.0 63.14209 1 ACCGGAA 1040 0.0 62.922157 3 >>END_MODULE