FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8266348
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC167770.20295540424864764No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT166490.20140695746174733Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG158100.19125737266323653No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA127600.154360789069127No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA126560.1531026760547705No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC114440.13844082054130796No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG105670.12783154060293617No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC97310.11771824752599333No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA93150.11268579546856725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC59200.087.701739
CGGGAGA85050.085.0820854
AGAGCGG64900.084.089388
GAGAGCG81300.077.682687
ATTCGGA10650.074.364252
TCGGGAG94600.073.98663
CGGAAAG30300.073.868155
AAGCGGC24500.073.348119
TCGGGAA31150.071.8524863
GAACCGG10800.068.067041
AAGCGTC14400.066.512729
AACCGGA10950.065.830452
AGAGCGT204500.065.290488
GATCGGG137600.064.350921
GAGCGTC204500.064.1087959
TCGGAGA18850.063.8969654
ATCGGGA135300.063.687542
CGGAGAG34650.063.4998365
GATCCGG16750.063.142091
ACCGGAA10400.062.9221573
Produced by FastQC (version 0.11.2)