FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34298-1.342000000357ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34298-1.342000000357ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5285485
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC140070.26500879294899143No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG131210.2482459036398741No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA93850.1775617563951085No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA92610.17521570868141714No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76290.14433869361089854No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74040.14008175219492627No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT68470.1295434572229417Illumina Single End PCR Primer 1 (96% over 32bp)
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA66960.12668657653933366No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT60640.11472930109535832No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG57530.10884526207150338No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC57520.10882634233187682No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT55440.10489103648955583No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT55350.10472075883291693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA21400.077.1115954
GAGCGGC16600.074.264719
GAGAGCG20400.069.9599467
AGAGCGG18300.069.178838
AAGCGGC6400.065.195859
CGGAAAG9500.064.390295
ATTCGGA3200.062.2412382
TCGGGAA8050.058.905843
TCGGGAG26700.058.43043
TCGGAGA4900.056.1288454
AAGCGTC3550.052.089899
CGGTGCG558.284587E-751.7378771
GATCCGG5400.050.9394531
AAAGCGG9300.050.4737248
CGACGGG2400.049.4024851
GAACCGG4150.049.1405941
CGGAGAG11900.049.0131235
GGATCGG7200.048.7437861
AACCGGA3800.047.4218942
TTCGGAG3250.046.689683
Produced by FastQC (version 0.11.2)