##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34274-2.3420000003583a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4579906 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.201394744782974 33.0 33.0 33.0 33.0 33.0 2 32.198610408161215 33.0 33.0 33.0 33.0 33.0 3 32.15323087417078 33.0 33.0 33.0 33.0 33.0 4 35.96613118260506 37.0 37.0 37.0 33.0 37.0 5 35.953301661649824 37.0 37.0 37.0 33.0 37.0 6 35.98937947634733 37.0 37.0 37.0 33.0 37.0 7 35.90616117448699 37.0 37.0 37.0 33.0 37.0 8 35.86910670218996 37.0 37.0 37.0 33.0 37.0 9 35.848626587532586 37.0 37.0 37.0 33.0 37.0 10-11 35.78290438712061 37.0 37.0 37.0 33.0 37.0 12-13 35.759956972916044 37.0 37.0 37.0 33.0 37.0 14-15 37.56552427058547 40.0 37.0 40.0 33.0 40.0 16-17 37.5416530382938 40.0 37.0 40.0 33.0 40.0 18-19 37.542544104616994 40.0 37.0 40.0 33.0 40.0 20-21 37.51033187144016 40.0 37.0 40.0 33.0 40.0 22-23 37.502085304807565 40.0 37.0 40.0 33.0 40.0 24-25 37.27179815480929 40.0 37.0 40.0 33.0 40.0 26-27 37.2014386321466 40.0 37.0 40.0 33.0 40.0 28-29 37.099493635895584 40.0 37.0 40.0 33.0 40.0 30-31 37.1485835080458 40.0 37.0 40.0 33.0 40.0 32-33 37.08417509005643 40.0 37.0 40.0 33.0 40.0 34-35 37.01037139190193 40.0 37.0 40.0 33.0 40.0 36-37 36.94785820931696 40.0 37.0 40.0 33.0 40.0 38-39 36.907989487120474 40.0 37.0 40.0 33.0 40.0 40-41 36.78425004792675 40.0 37.0 40.0 33.0 40.0 42-43 36.66694043502203 40.0 37.0 40.0 33.0 40.0 44-45 36.56228435692785 40.0 37.0 40.0 33.0 40.0 46-47 36.43966087950277 40.0 37.0 40.0 33.0 40.0 48-49 36.30759768868619 37.0 37.0 40.0 33.0 40.0 50-51 35.17526014726066 37.0 35.0 38.5 30.0 38.5 52-53 35.59779087169038 37.0 37.0 38.5 33.0 40.0 54-55 36.32144480694582 37.0 37.0 40.0 33.0 40.0 56-57 36.370026917583026 37.0 37.0 40.0 33.0 40.0 58-59 36.1976634454943 37.0 37.0 40.0 33.0 40.0 60-61 35.99249187647082 37.0 37.0 40.0 33.0 40.0 62-63 35.751363674276284 37.0 37.0 40.0 27.0 40.0 64-65 35.48814538988354 37.0 37.0 40.0 27.0 40.0 66-67 35.24108562490147 37.0 35.0 40.0 27.0 40.0 68-69 35.01606277508752 37.0 33.0 37.0 27.0 40.0 70-71 34.71489578607071 37.0 33.0 37.0 27.0 40.0 72-73 34.46861256541073 37.0 33.0 37.0 27.0 40.0 74-75 34.2229356890731 37.0 33.0 37.0 27.0 38.5 76-77 33.97146797336015 37.0 33.0 37.0 27.0 37.0 78-79 33.71215435425967 37.0 33.0 37.0 27.0 37.0 80-81 33.45291999442783 37.0 33.0 37.0 27.0 37.0 82-83 33.23167276795637 37.0 33.0 37.0 27.0 37.0 84-85 33.03646897993103 37.0 33.0 37.0 27.0 37.0 86-87 32.8808632753598 37.0 33.0 37.0 27.0 37.0 88-89 32.72123991191086 37.0 33.0 37.0 27.0 37.0 90-91 32.572284234654596 35.0 33.0 37.0 27.0 37.0 92-93 32.42399756676229 33.0 33.0 37.0 22.0 37.0 94-95 32.271238317991674 33.0 33.0 37.0 22.0 37.0 96-97 32.136612192477315 33.0 33.0 37.0 22.0 37.0 98-99 31.971157595810915 33.0 33.0 37.0 22.0 37.0 100-101 30.67278848081161 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.40139581098298294 2306 2 0.3522742708515523 2306 3 0.39457712456795235 2306 4 0.46422299167359427 2306 5 0.4718041438213021 2306 6 0.4370726281474191 2306 7 0.4765288087347628 2306 8 0.48983671067581724 2306 9 0.4334894239610847 2306 10-11 0.5010884226543766 2306 12-13 0.4583413632699447 2306 14-15 0.7205982768431838 2306 16-17 0.6238958392400349 2306 18-19 0.6353579611209952 2306 20-21 0.7566025949599506 2306 22-23 0.6795905288366342 2306 24-25 0.8645533046867229 2306 26-27 0.7893048322505933 2306 28-29 0.695349906640196 2306 30-31 0.6795291274545932 2306 32-33 0.5494384893200603 2306 34-35 0.7080670541610345 2306 36-37 0.6479479996358251 2306 38-39 0.6351579794178903 2306 40-41 0.6406229333407154 2306 42-43 0.646011316103646 2306 44-45 0.6751964972514983 2306 46-47 0.5857251458579498 2306 48-49 0.6470208824117663 2306 50-51 0.670821264650705 2306 52-53 0.6700115301604583 2306 54-55 0.781464574671972 2306 56-57 0.6088088778964718 2306 58-59 0.7867728888511607 2306 60-61 0.7811069578815122 2306 62-63 0.7179341216290638 2306 64-65 0.762101651154623 2306 66-67 0.6567948869125502 2306 68-69 0.71673269791971 2306 70-71 0.6734190707746635 2306 72-73 0.5209354204607664 2306 74-75 0.6029482168101907 2306 76-77 0.6094714780828028 2306 78-79 0.537854888141247 2306 80-81 0.5177626039203673 2306 82-83 0.5748679189204751 2306 84-85 0.6495545315719724 2306 86-87 0.6829575507394381 2306 88-89 0.6949559218105819 2306 90-91 0.59345201507859 2306 92-93 0.49124016597819065 2306 94-95 0.5628035647127376 2306 96-97 0.4218492604085142 2306 98-99 0.5497957134535838 2306 100-101 0.5376678640086006 2307 1 0.26476205093061367 2307 2 0.23582560523013285 2307 3 0.24304032720915814 2307 4 0.3286718526008059 2307 5 0.30604534767654457 2307 6 0.31443578298693353 2307 7 0.3789015932347155 2307 8 0.22637281783359242 2307 9 0.31576234933438485 2307 10-11 0.3004780920631589 2307 12-13 0.25851141192158167 2307 14-15 0.5351586248250513 2307 16-17 0.5146091695232187 2307 18-19 0.5069862387469684 2307 20-21 0.4997520334256649 2307 22-23 0.5080362142363839 2307 24-25 0.5550311829907173 2307 26-27 0.6008299835975137 2307 28-29 0.5421674168892281 2307 30-31 0.5508152582720953 2307 32-33 0.43867780341094686 2307 34-35 0.4928183135518438 2307 36-37 0.48576070741195565 2307 38-39 0.4251985294809728 2307 40-41 0.36579634257807214 2307 42-43 0.32078027960256605 2307 44-45 0.23501100497541927 2307 46-47 0.22044406526237026 2307 48-49 0.27505883683679144 2307 50-51 0.27165426795342285 2307 52-53 0.29596664479109336 2307 54-55 0.29138466205323255 2307 56-57 0.3715852220252671 2307 58-59 0.3078341467530592 2307 60-61 0.30423580484796986 2307 62-63 0.2993866421286313 2307 64-65 0.3953322681099394 2307 66-67 0.4107001286426524 2307 68-69 0.37203341156948255 2307 70-71 0.37706309101172764 2307 72-73 0.2882892138838997 2307 74-75 0.4076750388655128 2307 76-77 0.3244793415519993 2307 78-79 0.3910519655468434 2307 80-81 0.34528341996099954 2307 82-83 0.3897568508087872 2307 84-85 0.37641492470324067 2307 86-87 0.34345562872552193 2307 88-89 0.34800410869169696 2307 90-91 0.245329753654552 2307 92-93 0.37678548807300416 2307 94-95 0.37338027739079394 2307 96-97 0.6232056711703464 2307 98-99 0.47986611395703704 2307 100-101 0.17696851255643864 2201 1 0.32271654282065043 2201 2 0.29455639090250685 2201 3 0.33895226922768273 2201 4 0.36041830230831096 2201 5 0.3774253659135951 2201 6 0.3661259611476524 2201 7 0.4447974924985729 2201 8 0.3992851060635445 2201 9 0.4283938649764707 2201 10-11 0.31515339288512223 2201 12-13 0.4118138300376941 2201 14-15 0.5750698126553146 2201 16-17 0.5693058606847501 2201 18-19 0.6006482941375495 2201 20-21 0.5468728709647337 2201 22-23 0.5125863750702777 2201 24-25 0.6777925522975323 2201 26-27 0.6275796109732497 2201 28-29 0.5430821952407783 2201 30-31 0.5765000823463069 2201 32-33 0.43873387990904433 2201 34-35 0.49267088925105185 2201 36-37 0.5168083951910134 2201 38-39 0.48592531340440814 2201 40-41 0.5379073057772601 2201 42-43 0.48606843965951896 2201 44-45 0.4530209882174887 2201 46-47 0.4339043752922578 2201 48-49 0.3785188380395965 2201 50-51 0.39768611615352256 2201 52-53 0.5457381940140706 2201 54-55 0.6501744499540365 2201 56-57 0.6952064309695558 2201 58-59 0.6910025826738746 2201 60-61 0.6520541039908991 2201 62-63 0.6537302951630224 2201 64-65 0.6155518279175567 2201 66-67 0.5064968293940737 2201 68-69 0.628760071962077 2201 70-71 0.5208126745014994 2201 72-73 0.5662046635280262 2201 74-75 0.5030395442301696 2201 76-77 0.5078584300569631 2201 78-79 0.5142860028433986 2201 80-81 0.46965914225067706 2201 82-83 0.4697606217501047 2201 84-85 0.574323027513401 2201 86-87 0.4974634729336316 2201 88-89 0.44041752739133955 2201 90-91 0.5690523879171323 2201 92-93 0.5052783631069317 2201 94-95 0.3755882285506047 2201 96-97 0.5437317825008847 2201 98-99 0.4825291787245831 2201 100-101 0.43245059977245504 1101 1 0.31177738865193305 1101 2 0.24781566343980188 1101 3 0.26874082672745203 1101 4 0.30019526195483337 1101 5 0.3696913252184615 1101 6 0.38364406268479456 1101 7 0.32734414053513206 1101 8 0.4030662819970061 1101 9 0.31301054596809763 1101 10-11 0.3380271229814724 1101 12-13 0.32675906005668054 1101 14-15 0.4983211241166643 1101 16-17 0.4620035987203437 1101 18-19 0.48879101169688255 1101 20-21 0.5139736815121978 1101 22-23 0.535097384987445 1101 24-25 0.6068569142547346 1101 26-27 0.5996671058216236 1101 28-29 0.6058232107347123 1101 30-31 0.5740100198067566 1101 32-33 0.48157201401821936 1101 34-35 0.5267888970248293 1101 36-37 0.4819811775420604 1101 38-39 0.4975042071499942 1101 40-41 0.5090673170840248 1101 42-43 0.43408880905957403 1101 44-45 0.3468073176884445 1101 46-47 0.5235680279725585 1101 48-49 0.5605400684691801 1101 50-51 0.42602085325609096 1101 52-53 0.5783669232907585 1101 54-55 0.656213099378661 1101 56-57 0.6661235116811142 1101 58-59 0.6586677735635078 1101 60-61 0.62698696054062 1101 62-63 0.6693693472185416 1101 64-65 0.6341779112903438 1101 66-67 0.5641071449721196 1101 68-69 0.526604165866388 1101 70-71 0.5801306858756732 1101 72-73 0.5504676540429045 1101 74-75 0.5428732674781216 1101 76-77 0.5668911870980295 1101 78-79 0.5864614774437982 1101 80-81 0.5612965940475831 1101 82-83 0.5210872028194089 1101 84-85 0.6878755097144449 1101 86-87 0.6144099454703138 1101 88-89 0.7051935390627335 1101 90-91 0.6101275276812217 1101 92-93 0.494913164786702 1101 94-95 0.5811623797394176 1101 96-97 0.4762213509443072 1101 98-99 0.3935765552656605 1101 100-101 0.6008106947308356 2304 1 0.23260354904371638 2304 2 0.24371842145989575 2304 3 0.20110022864854926 2304 4 0.24391125968452343 2304 5 0.21409388538090468 2304 6 0.22865066636436637 2304 7 0.2958051618561015 2304 8 0.36045467656270347 2304 9 0.2545566085286666 2304 10-11 0.26178219940218383 2304 12-13 0.2884032444860125 2304 14-15 0.3868109629709906 2304 16-17 0.4744279217800056 2304 18-19 0.42761522275755937 2304 20-21 0.2977352641278799 2304 22-23 0.3922679465854415 2304 24-25 0.5863173586112396 2304 26-27 0.5158239340046435 2304 28-29 0.5640774130434352 2304 30-31 0.4636864629761206 2304 32-33 0.3736879643776163 2304 34-35 0.5092977211871528 2304 36-37 0.42163987490216925 2304 38-39 0.497627146072638 2304 40-41 0.5160951449680837 2304 42-43 0.4544416545991794 2304 44-45 0.48005501199693157 2304 46-47 0.36584149721146986 2304 48-49 0.37196029103736805 2304 50-51 0.3664807296744925 2304 52-53 0.4288074815092884 2304 54-55 0.6533591020764646 2304 56-57 0.6273049601435261 2304 58-59 0.7043139936158767 2304 60-61 0.5265323212120094 2304 62-63 0.5793160899328242 2304 64-65 0.6471540152854729 2304 66-67 0.6448695775091622 2304 68-69 0.5443472672056089 2304 70-71 0.6537884762678772 2304 72-73 0.6644209780514174 2304 74-75 0.6494943498605537 2304 76-77 0.5971229242593026 2304 78-79 0.5698171540086392 2304 80-81 0.5477080068956184 2304 82-83 0.57077343777609 2304 84-85 0.5366566632678698 2304 86-87 0.6239344173564803 2304 88-89 0.5298227455235818 2304 90-91 0.5092304616790884 2304 92-93 0.4712104141321163 2304 94-95 0.5470621735053172 2304 96-97 0.5336980503176605 2304 98-99 0.465408991499892 2304 100-101 0.5271738003449364 2202 1 0.20343716444065052 2202 2 0.20931091031207671 2202 3 0.20371071727295487 2202 4 0.19372772588029363 2202 5 0.21483104697427535 2202 6 0.19859795166485839 2202 7 0.06274231755600113 2202 8 0.18333543653361772 2202 9 0.21812094730124443 2202 10-11 0.18370204502512877 2202 12-13 0.20858284400083704 2202 14-15 0.3576639710697336 2202 16-17 0.3012694519478032 2202 18-19 0.24374652608251068 2202 20-21 0.3092196858550267 2202 22-23 0.21927895383520024 2202 24-25 0.2915722631330482 2202 26-27 0.25232693210837454 2202 28-29 0.3104917542450849 2202 30-31 0.31059497959769544 2202 32-33 0.1778547272777402 2202 34-35 0.3079270912150349 2202 36-37 0.28431063620309516 2202 38-39 0.29231521824693374 2202 40-41 0.28328380848041945 2202 42-43 0.3015045562411487 2202 44-45 0.20882087956747597 2202 46-47 0.3889302409227753 2202 48-49 0.3371900129213401 2202 50-51 0.35456258041519106 2202 52-53 0.39149767533277213 2202 54-55 0.42927089753801084 2202 56-57 0.43295229808703795 2202 58-59 0.5298120117374978 2202 60-61 0.4090456392594817 2202 62-63 0.3184715079032898 2202 64-65 0.2853485189079734 2202 66-67 0.48408408946215786 2202 68-69 0.3785518120266431 2202 70-71 0.3782700699258683 2202 72-73 0.5050194997461546 2202 74-75 0.38584204706680936 2202 76-77 0.364696495072927 2202 78-79 0.39469723384126354 2202 80-81 0.31536476324125573 2202 82-83 0.4025257814259149 2202 84-85 0.4511886859147296 2202 86-87 0.4223396364223291 2202 88-89 0.37423374496847117 2202 90-91 0.37871041074039624 2202 92-93 0.350374369669872 2202 94-95 0.3492907015634117 2202 96-97 0.3061519319242194 2202 98-99 0.3235155788861164 2202 100-101 0.3493997480188895 1102 1 0.1269164528489597 1102 2 0.1055454312502917 1102 3 0.20481209804573552 1102 4 0.12588111630067544 1102 5 0.14125155946274504 1102 6 0.18131255418683168 1102 7 0.14224375012799584 1102 8 0.25967355589843066 1102 9 0.11199666156383614 1102 10-11 0.18940400530161128 1102 12-13 0.16806307126987718 1102 14-15 0.11273349532633148 1102 16-17 0.1788509143043413 1102 18-19 0.23505429434577962 1102 20-21 0.19403976452508687 1102 22-23 0.22625323228835015 1102 24-25 0.18948313611166157 1102 26-27 0.3008121288644503 1102 28-29 0.16181052984118338 1102 30-31 0.25124667845002335 1102 32-33 0.20812586378485776 1102 34-35 0.26817961237986765 1102 36-37 0.26279453797884145 1102 38-39 0.18945169298672226 1102 40-41 0.2766389406493488 1102 42-43 0.1447392552391591 1102 44-45 0.24138348377292118 1102 46-47 0.2806674570388097 1102 48-49 0.15232768551793185 1102 50-51 0.07406320362447616 1102 52-53 0.13594463779453037 1102 54-55 0.3656595211357896 1102 56-57 0.42155501430828934 1102 58-59 0.41284032324501396 1102 60-61 0.4675675810824984 1102 62-63 0.4831334371002427 1102 64-65 0.38845404361781277 1102 66-67 0.3877113293734453 1102 68-69 0.3618205042583824 1102 70-71 0.25776648444907124 1102 72-73 0.4348412780778972 1102 74-75 0.34865393498626673 1102 76-77 0.4311752816763601 1102 78-79 0.36280640068329717 1102 80-81 0.31649058676780584 1102 82-83 0.31863501301696573 1102 84-85 0.3499396773840431 1102 86-87 0.4118261767204956 1102 88-89 0.3185678822687663 1102 90-91 0.28121652142883846 1102 92-93 0.2599073673874628 1102 94-95 0.38257525730421804 1102 96-97 0.43470349642233685 1102 98-99 0.313390451411486 1102 100-101 0.3765522481402961 2305 1 0.13206920923212095 2305 2 0.12580883192459424 2305 3 0.11724557034850847 2305 4 0.12253315583745916 2305 5 0.19905861334347463 2305 6 0.18643683330336103 2305 7 0.1162000260092384 2305 8 0.21057043533484432 2305 9 0.10340579479054668 2305 10-11 0.050409949299123014 2305 12-13 0.0824434449848539 2305 14-15 -0.02595553938139261 2305 16-17 0.08421118402393546 2305 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-0.7463533060822485 1213 66-67 -0.6856377302832541 1213 68-69 -0.6396631125461951 1213 70-71 -0.663860474670436 1213 72-73 -0.606905127293345 1213 74-75 -0.6528539866523673 1213 76-77 -0.8444367334617908 1213 78-79 -0.7916881882124471 1213 80-81 -0.696260594409658 1213 82-83 -0.6225740971229428 1213 84-85 -0.6608405810272728 1213 86-87 -0.8130392354738234 1213 88-89 -0.6556598644159308 1213 90-91 -0.6707968624104055 1213 92-93 -0.5609404744727158 1213 94-95 -0.5558400232318164 1213 96-97 -0.5708621572874328 1213 98-99 -0.8135110143726862 1213 100-101 -1.1183423741383969 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2318.0 3 178.0 4 136.0 5 233.0 6 385.0 7 1390.0 8 5702.0 9 8395.0 10 11281.0 11 10888.0 12 9168.0 13 8232.0 14 7899.0 15 8303.0 16 8701.0 17 9388.0 18 10200.0 19 11666.0 20 13209.0 21 15562.0 22 17572.0 23 19480.0 24 22539.0 25 26683.0 26 31837.0 27 38540.0 28 47420.0 29 56309.0 30 68957.0 31 89774.0 32 122606.0 33 175563.0 34 264436.0 35 442871.0 36 869355.0 37 1579212.0 38 562957.0 39 561.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.07256049587887 32.757135236092125 16.1100919678656 18.06021230016341 2 30.634855091228484 25.69493046758537 29.931854209388103 13.738360231798044 3 31.90991745005593 23.823407487478455 25.288408812440476 18.978266250025143 4 28.077652871219637 23.311821148986326 29.500850131733795 19.10967584806024 5 29.27855722377528 21.801152532959577 30.55404015170316 18.366250091561977 6 27.417118115447103 21.98357091666027 32.05206516083867 18.54724580705395 7 31.533038422383846 22.821336614786127 25.29539197268488 20.350232990145148 8 27.52545983586684 27.175916583606053 25.14234355326851 20.156280027258603 9 26.035891930272832 24.75120470852317 28.505317594242495 20.707585766961508 10-11 22.81735584103667 29.04452644139291 30.84823344080952 17.2898842767609 12-13 22.83336787945415 28.46982986691435 29.977472868176374 18.719329385455122 14-15 22.229923165959516 28.506889526633923 30.699366428539882 18.563820878866682 16-17 22.359246071027567 28.35365080503272 30.968520562176227 18.31858256176348 18-19 21.908708150560646 28.34691947748845 31.13630095951767 18.608071412433233 20-21 21.98588552231535 29.019570724803973 30.432483081546195 18.562060671334475 22-23 22.230765522587088 28.94725709592816 30.24770311960787 18.574274261876887 24-25 21.55234361757252 29.477724937443522 30.24991453373796 18.720016911246002 26-27 21.433401664814646 29.39720419512002 30.460198782955683 18.70919535710965 28-29 21.193068100642083 29.67070090999841 30.326232110703266 18.80999887865625 30-31 21.186458297541638 29.596819005603308 30.545921738363496 18.670800958491554 32-33 20.58837181297689 29.645034867045446 31.054539284267683 18.712054035709986 34-35 20.591433903410113 30.30585538067556 30.622358301495638 18.480352414418686 36-37 20.740475710575332 29.922876830105572 30.809700770684284 18.526946688634812 38-39 20.857085727188448 29.196735633200916 31.034151980133295 18.912026659477345 40-41 21.04500254853299 29.67278588326241 30.066656045498018 19.21555552270658 42-43 21.409838773962548 29.219138002397543 30.34816506534737 19.022858158292543 44-45 20.772532668192863 29.510615212230334 30.542401993231433 19.17445012634537 46-47 21.085713960262243 29.709343474669332 30.15033255722409 19.05461000784433 48-49 20.76641854888942 29.84237925821397 30.169710281486502 19.221491911410112 50-51 20.87084801172022 29.36264074712898 30.076797845918886 19.689713395231916 52-53 21.398067133982966 29.2407048430743 29.764239590916052 19.59698843202668 54-55 21.49817971564251 29.395886797969517 29.692717165207572 19.4132163211804 56-57 21.470252005713494 29.176502568377817 29.877678145274402 19.475567280634294 58-59 21.401295259356885 29.47906076228049 29.851891660654882 19.267752317707735 60-61 20.772428998794112 29.63682031715275 29.588050495926886 20.002700188126248 62-63 21.039098773104747 29.70043048418412 29.451135950049995 19.80933479266114 64-65 20.69104133525899 29.69525951828768 29.830036384716774 19.783662761736554 66-67 20.88662076221423 29.238678222461573 29.80025420062314 20.074446814701062 68-69 20.740356438228186 29.24187180111046 30.565698246234916 19.452073514426445 70-71 20.736859240281124 30.258517928918376 29.795567078666284 19.209055752134212 72-73 20.64162099255057 30.545015749141612 29.428991818502638 19.38437143980518 74-75 20.797566791396633 30.131985627920244 29.434728264046573 19.635719316636553 76-77 20.911982387457545 29.923315119752942 29.490302899475314 19.674399593314202 78-79 20.65062799222772 29.75242960380133 29.943693549469362 19.653248854501587 80-81 20.61752303299937 30.03084933090451 29.67511866811462 19.676508967981505 82-83 20.311992449343773 30.07627319304574 29.59618708507477 20.015547272535713 84-85 20.40167735761085 30.071137758366007 29.693166050276698 19.834018833746445 86-87 20.43766395533253 29.901510168652912 30.082897305509032 19.577928570505527 88-89 20.63253429349685 30.30765441341799 29.68026322721002 19.379548065875145 90-91 20.919569277511783 29.953132875082357 29.33555943873325 19.791738408672614 92-93 20.561473182595872 30.246438366542254 29.329125891749463 19.86296255911241 94-95 20.15787484356513 30.54646382483629 29.33282586753282 19.962835464065762 96-97 20.37014800162397 30.724296034772813 29.136963867786864 19.768592095816352 98-99 20.618139867463878 30.319539996065103 28.99728816298306 20.065031973487958 100-101 20.616873679131572 30.218721523628677 28.93685987525933 20.227544921980424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1926.0 1 1121.0 2 272.0 3 225.0 4 214.0 5 247.5 6 361.5 7 560.5 8 840.0 9 1124.5 10 1376.0 11 1575.0 12 1614.0 13 1509.5 14 1354.0 15 1184.5 16 1041.5 17 972.5 18 1056.5 19 1272.5 20 1612.0 21 2210.5 22 3076.5 23 4425.5 24 6546.5 25 9485.5 26 13768.0 27 19730.5 28 26409.5 29 33578.0 30 41340.0 31 50134.0 32 64323.0 33 86837.0 34 114975.0 35 151790.5 36 198768.0 37 254643.0 38 306462.0 39 323132.0 40 328532.0 41 315115.5 42 280628.0 43 266924.0 44 250181.5 45 234228.5 46 236381.0 47 218181.0 48 172451.5 49 129826.0 50 98656.5 51 79024.5 52 63237.5 53 47636.5 54 36545.5 55 28072.0 56 19598.5 57 12927.5 58 8551.0 59 5910.5 60 4316.5 61 3192.0 62 2200.0 63 1413.0 64 956.5 65 681.0 66 510.0 67 398.5 68 281.5 69 199.0 70 163.5 71 126.0 72 104.5 73 97.0 74 86.5 75 74.0 76 55.5 77 46.0 78 43.0 79 38.0 80 32.0 81 23.5 82 18.5 83 17.0 84 12.5 85 15.0 86 13.5 87 8.5 88 9.0 89 9.5 90 5.0 91 3.5 92 3.0 93 2.5 94 3.0 95 1.5 96 2.0 97 1.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1743048874802234 2 0.16703399589423887 3 0.13515561236409657 4 0.12902011526000753 5 0.1419461447462022 6 0.1616845411237698 7 0.1380377675873697 8 0.1296314815194897 9 0.12146537505354914 10-11 0.10784064127080338 12-13 0.13777575347616305 14-15 0.13432590101194217 16-17 0.1240636816563484 18-19 0.12176014092865663 20-21 0.11444558032413765 22-23 0.1271095956991257 24-25 0.42854591338774206 26-27 0.30944958258968636 28-29 0.4020715709012368 30-31 0.23238468213103064 32-33 0.27415409835922394 34-35 0.28654518236837173 36-37 0.2836411926358314 38-39 0.3029538160826882 40-41 0.2959340213532767 42-43 0.35077139137790164 44-45 0.29809563777073156 46-47 0.32374026890508234 48-49 0.32320532342803543 50-51 0.32323807519193626 52-53 0.326971776276631 54-55 0.3056503779771899 56-57 0.31819430355120826 58-59 0.3180305447317041 60-61 0.2960213593903456 62-63 0.29301911436610273 64-65 0.30220052551296905 66-67 0.3019166768924952 68-69 0.2985432452107096 70-71 0.2721999097798077 72-73 0.2628438225588036 74-75 0.2715994607749591 76-77 0.26751640754198885 78-79 0.26495085270309043 80-81 0.2739357532665518 82-83 0.3160545216430206 84-85 0.3220480944368727 86-87 0.3112945986227665 88-89 0.31818338629657467 90-91 0.29444927472310567 92-93 0.278608338249737 94-95 0.2644268244806771 96-97 0.2638591272397294 98-99 0.28549712592354515 100-101 0.28540978788647625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4579906.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.88516820306671 #Duplication Level Percentage of deduplicated Percentage of total 1 89.54955269614318 43.77644946060346 2 5.082706902123568 4.969379636743974 3 1.600639676213505 2.347426194125983 4 0.6637795677649805 1.2979590327979995 5 0.5132206261931006 1.2544438318366473 6 0.29814634908060644 0.8744960654361413 7 0.2590866953242971 0.8865847676073475 8 0.1895831711584461 0.7414244168441139 9 0.15335015101931523 0.6746893133890417 >10 1.392300822437621 13.767610111626317 >50 0.1619475431097701 5.50183974685657 >100 0.10771608723968372 10.411450545206788 >500 0.018312281016184363 6.3998850380790255 >1k 0.00961219187610477 6.870232816557905 >5k 0.0 0.0 >10k+ 4.523929980446327E-5 0.22612902228875786 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 10225 0.22325785725733235 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.38883898490493E-4 0.0 0.0 2 0.0011572289911627007 0.0 0.0 3 0.0018340987784465446 0.0 0.0 4 0.0027729826769370375 0.0 0.0 5 0.0033406799178847773 0.0 0.0 6 0.005436792807537972 0.0 0.0 7 0.006812366891372879 0.0 0.0 8 0.008428120577147216 0.0 0.0 9 0.00934516996637049 0.0 2.183450926722077E-5 10-11 0.013286298889103838 0.0 2.183450926722077E-5 12-13 0.01990215519707173 0.0 2.183450926722077E-5 14-15 0.028646876158593647 0.0 2.183450926722077E-5 16-17 0.04023008332485427 0.0 2.183450926722077E-5 18-19 0.053570968487126155 0.0 2.183450926722077E-5 20-21 0.06794899283959102 0.0 2.183450926722077E-5 22-23 0.08627906336942287 0.0 2.183450926722077E-5 24-25 0.10562443858018047 0.0 2.183450926722077E-5 26-27 0.12705500942595765 0.0 2.183450926722077E-5 28-29 0.14821264890589458 0.0 2.183450926722077E-5 30-31 0.17156465656718717 0.0 2.183450926722077E-5 32-33 0.19508042304798395 0.0 2.183450926722077E-5 34-35 0.2202665294877231 0.0 2.183450926722077E-5 36-37 0.24783259743758934 0.0 2.183450926722077E-5 38-39 0.281130224070101 0.0 2.183450926722077E-5 40-41 0.3160217698791198 0.0 2.183450926722077E-5 42-43 0.3546142650089325 0.0 2.183450926722077E-5 44-45 0.3955212181210706 0.0 2.183450926722077E-5 46-47 0.4398561891881624 0.0 2.183450926722077E-5 48-49 0.4894860287525552 0.0 2.183450926722077E-5 50-51 0.5419761890309539 0.0 2.183450926722077E-5 52-53 0.5993572793852101 0.0 2.183450926722077E-5 54-55 0.6576772536379567 0.0 2.183450926722077E-5 56-57 0.719709094466131 0.0 2.183450926722077E-5 58-59 0.7844811662073414 0.0 4.366901853444154E-5 60-61 0.8527249249220399 0.0 5.458627316805192E-5 62-63 0.9240364321887828 0.0 9.825529170249346E-5 64-65 0.9982519291880663 0.0 1.2008980096971423E-4 66-67 1.075960947670105 0.0 1.310070556033246E-4 68-69 1.1566940456856538 0.0 1.41924310236935E-4 70-71 1.2412591000775999 0.0 1.6375881950415577E-4 72-73 1.3300382147581193 0.0 2.183450926722077E-4 74-75 1.4220488368101878 0.0 2.183450926722077E-4 76-77 1.5178586634747524 0.0 2.2926234730581807E-4 78-79 1.621157726817974 0.0 2.4017960193942846E-4 80-81 1.726432813249879 0.0 2.5109685657303887E-4 82-83 1.8403106963330689 0.0 2.620141112066492E-4 84-85 1.9604987525944857 0.0 2.620141112066492E-4 86-87 2.085905256570768 0.0 2.620141112066492E-4 88-89 2.2188228317349745 0.0 2.620141112066492E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTCG 30 1.3120641E-4 63.231285 1 ATCGGGA 755 0.0 59.038624 2 GAGCGGC 530 0.0 57.25167 9 CGGTGCG 35 2.8111215E-4 54.19825 1 CGAGGCG 175 0.0 48.77842 1 CGGGAGA 880 0.0 46.33806 4 GAGAGCG 950 0.0 41.42181 7 AGAGCGG 715 0.0 41.111206 8 CGACGGG 135 7.2759576E-12 38.641342 1 CGAGCCC 185 0.0 38.451458 1 CGGAGCG 65 1.3414695E-4 36.479588 1 CGGGATG 210 0.0 36.132168 1 CGGAAAG 500 0.0 36.035923 5 GATCGGG 1545 0.0 35.912918 1 GATCCGG 200 0.0 35.5676 1 CGTGCGG 95 8.8179877E-7 34.943607 1 CGACGGC 70 2.0738356E-4 33.873905 1 CGATAGG 85 1.6513855E-5 33.47539 1 CGGGTGC 130 8.431016E-9 32.83163 1 CTCGCCG 75 3.109138E-4 31.615644 1 >>END_MODULE