FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34274-2.3420000003583a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34274-2.3420000003583a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4579906
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT102250.22325785725733235Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG301.3120641E-463.2312851
ATCGGGA7550.059.0386242
GAGCGGC5300.057.251679
CGGTGCG352.8111215E-454.198251
CGAGGCG1750.048.778421
CGGGAGA8800.046.338064
GAGAGCG9500.041.421817
AGAGCGG7150.041.1112068
CGACGGG1357.2759576E-1238.6413421
CGAGCCC1850.038.4514581
CGGAGCG651.3414695E-436.4795881
CGGGATG2100.036.1321681
CGGAAAG5000.036.0359235
GATCGGG15450.035.9129181
GATCCGG2000.035.56761
CGTGCGG958.8179877E-734.9436071
CGACGGC702.0738356E-433.8739051
CGATAGG851.6513855E-533.475391
CGGGTGC1308.431016E-932.831631
CTCGCCG753.109138E-431.6156441
Produced by FastQC (version 0.11.2)