##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34271-1.342000000358da.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5161012 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.102479707468227 33.0 33.0 33.0 27.0 33.0 2 30.670277263451432 33.0 33.0 33.0 27.0 33.0 3 30.49403585963373 33.0 33.0 33.0 22.0 33.0 4 33.91315501688428 37.0 37.0 37.0 27.0 37.0 5 33.97014694017375 37.0 37.0 37.0 27.0 37.0 6 34.53648528621906 37.0 37.0 37.0 27.0 37.0 7 33.90046428878677 37.0 37.0 37.0 22.0 37.0 8 34.20187436107492 37.0 37.0 37.0 27.0 37.0 9 33.70810065932805 37.0 37.0 37.0 22.0 37.0 10-11 33.72154550309125 37.0 37.0 37.0 22.0 37.0 12-13 33.6833901955663 37.0 37.0 37.0 22.0 37.0 14-15 35.176638515856965 40.0 37.0 40.0 22.0 40.0 16-17 35.0978001019955 40.0 37.0 40.0 18.5 40.0 18-19 35.146610587225915 40.0 37.0 40.0 18.5 40.0 20-21 35.12295311462171 40.0 37.0 40.0 18.5 40.0 22-23 35.82141661364089 40.0 37.0 40.0 27.0 40.0 24-25 35.098195663951174 38.5 37.0 40.0 22.0 40.0 26-27 34.99303615259953 37.0 37.0 40.0 21.0 40.0 28-29 34.65115175085816 37.0 37.0 40.0 15.0 40.0 30-31 34.65872914071891 37.0 37.0 40.0 18.5 40.0 32-33 34.520262111384355 37.0 37.0 40.0 15.0 40.0 34-35 34.51738583440612 37.0 37.0 40.0 15.0 40.0 36-37 34.35204239013589 37.0 37.0 40.0 15.0 40.0 38-39 34.2951661418342 37.0 37.0 40.0 15.0 40.0 40-41 34.30034497110257 37.0 33.0 40.0 15.0 40.0 42-43 33.924822011651976 37.0 33.0 40.0 15.0 40.0 44-45 33.74567972327908 37.0 33.0 40.0 15.0 40.0 46-47 33.65638822385997 37.0 33.0 40.0 15.0 40.0 48-49 33.53650534042548 37.0 33.0 40.0 15.0 40.0 50-51 32.564524263845925 37.0 33.0 38.5 15.0 38.5 52-53 33.01261874609088 37.0 33.0 38.5 15.0 40.0 54-55 33.61549294983232 37.0 33.0 40.0 15.0 40.0 56-57 33.828688346394074 37.0 33.0 40.0 18.5 40.0 58-59 33.36892774130345 37.0 33.0 40.0 15.0 40.0 60-61 33.145774510890504 37.0 33.0 40.0 15.0 40.0 62-63 32.878439732362565 37.0 33.0 40.0 10.5 40.0 64-65 32.631249065105834 37.0 33.0 38.5 10.5 40.0 66-67 32.487202800536025 37.0 33.0 37.0 15.0 40.0 68-69 32.46895870422313 37.0 33.0 37.0 15.0 40.0 70-71 32.114143602068744 37.0 33.0 37.0 15.0 40.0 72-73 32.2063258136195 37.0 33.0 37.0 15.0 38.5 74-75 32.239473188591695 37.0 33.0 37.0 18.5 37.0 76-77 31.985760060236245 37.0 33.0 37.0 22.0 37.0 78-79 31.628632620889082 37.0 33.0 37.0 15.0 37.0 80-81 31.224929626205093 33.0 33.0 37.0 15.0 37.0 82-83 30.902159595831208 33.0 30.0 37.0 15.0 37.0 84-85 30.654915838211576 33.0 27.0 37.0 15.0 37.0 86-87 30.48347397758424 33.0 27.0 37.0 15.0 37.0 88-89 30.323779231670066 33.0 27.0 37.0 15.0 37.0 90-91 30.179866371169066 33.0 27.0 37.0 15.0 37.0 92-93 30.02105565730132 33.0 27.0 37.0 15.0 37.0 94-95 29.862781853636456 33.0 27.0 37.0 15.0 37.0 96-97 29.72539213239574 33.0 27.0 37.0 10.5 37.0 98-99 29.551585813014967 33.0 27.0 37.0 6.0 37.0 100-101 28.30651788835213 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14762398280802103 2306 2 0.06593020214541667 2306 3 0.04461722273665458 2306 4 0.13205223731877425 2306 5 0.11229951383271697 2306 6 -0.07195794868715666 2306 7 0.5421154210085533 2306 8 -0.06697106566720379 2306 9 0.16683367968056473 2306 10-11 0.13107809925625702 2306 12-13 0.14978000377649892 2306 14-15 0.5304820061529725 2306 16-17 0.4215529840616412 2306 18-19 0.4352920417019206 2306 20-21 0.33121746119165607 2306 22-23 0.249846310440482 2306 24-25 0.5940438135080015 2306 26-27 0.33713308572384193 2306 28-29 0.5470696704386526 2306 30-31 0.5806573083888935 2306 32-33 0.5529011807136257 2306 34-35 0.4102270279100111 2306 36-37 0.3474550559104017 2306 38-39 0.2963731355100947 2306 40-41 0.19043168206682282 2306 42-43 0.39097589657912835 2306 44-45 0.5826109787430838 2306 46-47 0.4887052575753046 2306 48-49 0.446516257032485 2306 50-51 0.47933186745279244 2306 52-53 0.4976439340410579 2306 54-55 0.7278737916935825 2306 56-57 0.6354304033379705 2306 58-59 0.8198146188629636 2306 60-61 0.7077803074183677 2306 62-63 0.6836222889816952 2306 64-65 0.7090014480403397 2306 66-67 0.5852943724736335 2306 68-69 0.4562358947223544 2306 70-71 0.4571316421054661 2306 72-73 0.32511795497573814 2306 74-75 0.3329148002797595 2306 76-77 0.38434297627703984 2306 78-79 0.35329166193596606 2306 80-81 0.3928171956408484 2306 82-83 0.38784916805078495 2306 84-85 0.40344359554289966 2306 86-87 0.3812994867085173 2306 88-89 0.5278282935199137 2306 90-91 0.49543597467359035 2306 92-93 0.42872940708661744 2306 94-95 0.49305443121802384 2306 96-97 0.4494979300138269 2306 98-99 0.44926911904062905 2306 100-101 0.6111209807389493 2307 1 0.12728514513838363 2307 2 0.09002559455338499 2307 3 0.14754548465667483 2307 4 0.2889284606240281 2307 5 0.2715632380234112 2307 6 0.17362165151106268 2307 7 0.5627302398365472 2307 8 0.17869423511695004 2307 9 0.346259191414525 2307 10-11 0.2989289775608981 2307 12-13 0.31540943072648986 2307 14-15 0.5247500640580256 2307 16-17 0.5252958846823432 2307 18-19 0.5424142063634392 2307 20-21 0.5262346447909323 2307 22-23 0.37552536494226274 2307 24-25 0.6907210613106116 2307 26-27 0.4134383825775174 2307 28-29 0.5167990045005055 2307 30-31 0.5837165517248124 2307 32-33 0.49052750638760045 2307 34-35 0.364497366208802 2307 36-37 0.4173062735982853 2307 38-39 0.44961488838959696 2307 40-41 0.35610808453312615 2307 42-43 0.40142029313960137 2307 44-45 0.3718079033035977 2307 46-47 0.2654151682274275 2307 48-49 0.3219895892474156 2307 50-51 0.43885755447529107 2307 52-53 0.2718794168040546 2307 54-55 0.44260482304328264 2307 56-57 0.46849944983979697 2307 58-59 0.4306021745432602 2307 60-61 0.42475668668679845 2307 62-63 0.48926763111768423 2307 64-65 0.5576050822357459 2307 66-67 0.4775500795343106 2307 68-69 0.4892970326878867 2307 70-71 0.3932877530440919 2307 72-73 0.2921763741589345 2307 74-75 0.25193287522458263 2307 76-77 0.26521429331666013 2307 78-79 0.3232789153674709 2307 80-81 0.30894419513846216 2307 82-83 0.4063415408844584 2307 84-85 0.5396655688312215 2307 86-87 0.2682278208756905 2307 88-89 0.41200737072900395 2307 90-91 0.39216108249986803 2307 92-93 0.5816905332859683 2307 94-95 0.48882206355611757 2307 96-97 0.5626168315121909 2307 98-99 0.3827310131633155 2307 100-101 0.35398908278017416 2201 1 0.029564792900636405 2201 2 -0.05886461080602956 2201 3 -0.013228303964169186 2201 4 0.10679018526822404 2201 5 0.02780310851830592 2201 6 0.015222073132768799 2201 7 0.3487156770511177 2201 8 0.010029206152935899 2201 9 0.0847835127155463 2201 10-11 0.1048183729685519 2201 12-13 0.09919351823941724 2201 14-15 0.2499321806066206 2201 16-17 0.24122097190949177 2201 18-19 0.25675058398567074 2201 20-21 0.20293997055744484 2201 22-23 0.1811478072805386 2201 24-25 0.4384680850171563 2201 26-27 0.22633404455438466 2201 28-29 0.2828612086862563 2201 30-31 0.35305149361015964 2201 32-33 0.1716312747473907 2201 34-35 0.2109487804096304 2201 36-37 0.3227017019614209 2201 38-39 0.18953513688376944 2201 40-41 0.14774523731704647 2201 42-43 0.2512164702653976 2201 44-45 0.2555810525175204 2201 46-47 0.274330631113628 2201 48-49 0.22467983187605256 2201 50-51 0.2359699525127681 2201 52-53 0.300808459254732 2201 54-55 0.4320433368621792 2201 56-57 0.47156826931263396 2201 58-59 0.5319324378879102 2201 60-61 0.3805181121691916 2201 62-63 0.336455235068982 2201 64-65 0.4106109962729718 2201 66-67 0.3595859394467098 2201 68-69 0.4014493807170467 2201 70-71 0.3804086211118545 2201 72-73 0.28697356474606295 2201 74-75 0.13943427486371718 2201 76-77 0.15759985459851578 2201 78-79 0.24862025704315016 2201 80-81 0.3749557398796455 2201 82-83 0.3854118617512192 2201 84-85 0.32026559651070485 2201 86-87 0.28006127594244035 2201 88-89 0.2593957939493663 2201 90-91 0.41555215134467716 2201 92-93 0.41682159127148566 2201 94-95 0.2639219862115816 2201 96-97 0.48806680697934723 2201 98-99 0.38102600750891114 2201 100-101 0.30754779638172636 1101 1 0.27955406604650435 1101 2 0.1263637751441884 1101 3 0.30695045531798826 1101 4 0.3176515117410261 1101 5 0.3988120015793015 1101 6 0.1948388176023883 1101 7 0.4078966044051242 1101 8 0.2714668391004409 1101 9 0.40851529296671174 1101 10-11 0.3627481375190982 1101 12-13 0.4077409375454124 1101 14-15 0.5948248634202784 1101 16-17 0.5721022004386285 1101 18-19 0.6278770982495132 1101 20-21 0.5378951273179453 1101 22-23 0.4338995260094478 1101 24-25 0.6646282130010022 1101 26-27 0.637428100106014 1101 28-29 0.7049399682428259 1101 30-31 0.678582672459001 1101 32-33 0.6556912942403272 1101 34-35 0.5943413276408407 1101 36-37 0.6688855986583206 1101 38-39 0.6458813620663832 1101 40-41 0.5110575813567024 1101 42-43 0.6240884058464005 1101 44-45 0.5194680254017854 1101 46-47 0.5865906021611025 1101 48-49 0.6720247920382576 1101 50-51 0.48821068008285096 1101 52-53 0.6429782985340324 1101 54-55 0.8090901703073143 1101 56-57 0.76308047759521 1101 58-59 0.8413516011883004 1101 60-61 0.80748990752776 1101 62-63 0.7844473301258716 1101 64-65 0.8541701876777239 1101 66-67 0.7645435484159577 1101 68-69 0.6602343312400549 1101 70-71 0.7629393404522062 1101 72-73 0.6591868278276394 1101 74-75 0.6938563144577827 1101 76-77 0.7487898705915086 1101 78-79 0.6578143809223498 1101 80-81 0.6387903881673047 1101 82-83 0.6790003087796421 1101 84-85 0.837434893210748 1101 86-87 0.8268770716592329 1101 88-89 0.844465141814009 1101 90-91 0.7155202701363521 1101 92-93 0.7367168061216418 1101 94-95 0.686669240822944 1101 96-97 0.7831473469442329 1101 98-99 0.6658117922319242 1101 100-101 0.8066316793831803 2304 1 0.25307504571890505 2304 2 0.10531100953626549 2304 3 0.1423795076396317 2304 4 0.17745099250898022 2304 5 0.14317814893784941 2304 6 0.389159121045509 2304 7 0.31181219009913264 2304 8 0.26939884788315993 2304 9 0.17951974018085792 2304 10-11 0.2827547993913626 2304 12-13 0.2716866109740863 2304 14-15 0.3595911026948855 2304 16-17 0.3942815323703144 2304 18-19 0.37730676702857835 2304 20-21 0.32153211678111404 2304 22-23 0.3599949661584887 2304 24-25 0.4714580014439136 2304 26-27 0.47098258493584666 2304 28-29 0.5151353775569802 2304 30-31 0.4603617310929664 2304 32-33 0.3598157271073106 2304 34-35 0.3807102432920004 2304 36-37 0.32807428567969055 2304 38-39 0.39860716211868663 2304 40-41 0.3589748213475801 2304 42-43 0.4282539558946965 2304 44-45 0.40403012742068256 2304 46-47 0.41787475311532774 2304 48-49 0.5181090261637991 2304 50-51 0.4266083316347462 2304 52-53 0.4182446442952923 2304 54-55 0.571506345852093 2304 56-57 0.7379318797655685 2304 58-59 0.7283813947483395 2304 60-61 0.5563181038961105 2304 62-63 0.6266629095616096 2304 64-65 0.59798510294425 2304 66-67 0.5746485071384129 2304 68-69 0.6431646349150029 2304 70-71 0.6404312812804562 2304 72-73 0.61977127078233 2304 74-75 0.6136245975794239 2304 76-77 0.6167882830815188 2304 78-79 0.5433322201694288 2304 80-81 0.5413343200677367 2304 82-83 0.6115640773476905 2304 84-85 0.5618293877607101 2304 86-87 0.560937647369439 2304 88-89 0.6281241433235643 2304 90-91 0.6363636900720238 2304 92-93 0.6392464437054599 2304 94-95 0.6235216347065595 2304 96-97 0.5205222521542971 2304 98-99 0.5142435975908981 2304 100-101 0.45766744076034627 2202 1 0.2614725718835693 2202 2 0.20397446417319287 2202 3 0.21683396618876571 2202 4 0.3142720288919989 2202 5 0.22209602195901823 2202 6 0.34345329483574005 2202 7 0.3636409843228847 2202 8 0.35076328202715956 2202 9 0.21626757314499656 2202 10-11 0.2863728004585795 2202 12-13 0.3037934692234785 2202 14-15 0.3412837644934612 2202 16-17 0.3293573842099917 2202 18-19 0.2709092535039801 2202 20-21 0.3437784429076842 2202 22-23 0.3144604891243432 2202 24-25 0.3808027371341396 2202 26-27 0.3154592900017974 2202 28-29 0.38902551707388966 2202 30-31 0.3801919466703936 2202 32-33 0.43360900666733215 2202 34-35 0.3886974576469342 2202 36-37 0.46237403744405015 2202 38-39 0.3924200675610692 2202 40-41 0.38649066583423064 2202 42-43 0.3902092357757354 2202 44-45 0.4003004905948515 2202 46-47 0.4114980848036396 2202 48-49 0.42333476194914255 2202 50-51 0.573257864110289 2202 52-53 0.6044077870167897 2202 54-55 0.5524186742427588 2202 56-57 0.5986972717432906 2202 58-59 0.6200108050009803 2202 60-61 0.6819939252034928 2202 62-63 0.6379072020540519 2202 64-65 0.6087412001145509 2202 66-67 0.6278079135216714 2202 68-69 0.5532645600879889 2202 70-71 0.711229982012398 2202 72-73 0.6725299816132448 2202 74-75 0.5332511555357158 2202 76-77 0.5728408971398693 2202 78-79 0.5722458816512308 2202 80-81 0.468206578140407 2202 82-83 0.540513637707658 2202 84-85 0.6004535350601969 2202 86-87 0.5368852627936782 2202 88-89 0.505643534656425 2202 90-91 0.44807884138178977 2202 92-93 0.6485726878503044 2202 94-95 0.5762030014071975 2202 96-97 0.5130473963518476 2202 98-99 0.4731877630397783 2202 100-101 0.4418083798976653 1102 1 0.05302287636471803 1102 2 -0.11084645230362966 1102 3 0.00847751289570553 1102 4 -0.071421804391548 1102 5 -0.11783491503063459 1102 6 0.05955345029335746 1102 7 -0.041600679611896396 1102 8 0.10901603915311142 1102 9 0.02315834921094506 1102 10-11 0.10318953027629618 1102 12-13 0.042091188693291315 1102 14-15 -0.08493314935981999 1102 16-17 0.015133231839818961 1102 18-19 0.021906698121775037 1102 20-21 -0.04943682211069955 1102 22-23 0.061597229680160126 1102 24-25 0.07937744330907748 1102 26-27 0.1900963187629614 1102 28-29 0.043945349783108156 1102 30-31 0.1085335634526956 1102 32-33 0.11898028099238189 1102 34-35 0.03062596377755966 1102 36-37 0.1293123679692485 1102 38-39 0.03713502843330474 1102 40-41 0.004853262117343604 1102 42-43 -0.03334195769472359 1102 44-45 0.08527017185475216 1102 46-47 0.13852400665066966 1102 48-49 -0.0017636610420481702 1102 50-51 -0.02297961801694015 1102 52-53 0.0928330445707104 1102 54-55 0.2730912796722009 1102 56-57 0.313579271581105 1102 58-59 0.28720030734948665 1102 60-61 0.31451896672470525 1102 62-63 0.26757153697199954 1102 64-65 0.3402798747303706 1102 66-67 0.36949030730558263 1102 68-69 0.27491604716981044 1102 70-71 0.24433450687094194 1102 72-73 0.3696025315039577 1102 74-75 0.42246083988212746 1102 76-77 0.4081294062830807 1102 78-79 0.2693355274901812 1102 80-81 0.3131251194124509 1102 82-83 0.23621636319455064 1102 84-85 0.33558034887677124 1102 86-87 0.35645677364348316 1102 88-89 0.2583526533348781 1102 90-91 0.2695328933641008 1102 92-93 0.2622534894007664 1102 94-95 0.2344019062962026 1102 96-97 0.28521704083236443 1102 98-99 0.27489093533158737 1102 100-101 0.35915030186574626 2305 1 0.19816022343051287 2305 2 0.20026059949631403 2305 3 0.12653514067512717 2305 4 0.16852700742219184 2305 5 0.07131953181534101 2305 6 0.29665144056654214 2305 7 0.0646278838537313 2305 8 0.22903525413408943 2305 9 0.06908043740779135 2305 10-11 0.13708094397499337 2305 12-13 0.19960705780748356 2305 14-15 0.0768870266971291 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1213 84-85 -0.5940616948668342 1213 86-87 -0.7801025587687427 1213 88-89 -0.5648284847262239 1213 90-91 -0.5430921612618604 1213 92-93 -0.4884061567747615 1213 94-95 -0.4955419862268542 1213 96-97 -0.4151368346997302 1213 98-99 -0.6889880590656041 1213 100-101 -0.9651170577723001 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2253.0 3 301.0 4 461.0 5 444.0 6 936.0 7 8647.0 8 53117.0 9 97043.0 10 97819.0 11 74732.0 12 51416.0 13 36821.0 14 27636.0 15 22762.0 16 20213.0 17 19039.0 18 19041.0 19 19519.0 20 20869.0 21 22612.0 22 24505.0 23 26567.0 24 30895.0 25 35962.0 26 42531.0 27 50051.0 28 58509.0 29 69522.0 30 86675.0 31 113496.0 32 152812.0 33 216055.0 34 323654.0 35 529483.0 36 952615.0 37 1438472.0 38 412311.0 39 1216.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.79262842290123 23.617773497806873 21.545687945483934 15.04391013380796 2 31.550938396071604 26.66464585620006 29.068485292587997 12.715930455140336 3 35.153705642210696 20.51058161848751 28.60961498319621 15.726097756105586 4 28.9138709879538 18.496803825516206 32.281973335371454 20.30735185115854 5 34.48523457407119 17.145350987097252 30.6110539216352 17.758360517196355 6 31.52620498778368 17.33442957795811 34.76830900903121 16.371056425227003 7 35.47695447543738 19.408739412328455 27.146188438243268 17.968117673990896 8 28.57597886976686 30.0263545963805 23.980607082957814 17.417059450894826 9 32.35063635718245 25.422204670097866 24.011344913856007 18.215814058863668 10-11 27.094856755851765 28.895354401880734 27.65634980642735 16.353439035840154 12-13 28.635752837454802 24.130202380390006 27.342377660391236 19.891667121763955 14-15 25.51818920670369 24.478934833863146 28.958273175019443 21.044602784413716 16-17 27.494534782448454 24.76396273371443 29.618220366106534 18.123282117730582 18-19 27.006191443318457 24.852963040946314 29.86929390739438 18.27155160834085 20-21 25.713071257326902 27.291776637065052 28.526894326686346 18.4682577789217 22-23 28.71254987062299 25.04286540930719 27.534364586140025 18.71022013392979 24-25 24.674359160421997 28.744833178314906 27.738481151399476 18.84232650986363 26-27 23.154064338701826 30.291496970241088 27.746225481222726 18.808213209834367 28-29 24.472037705632562 29.192934064350318 27.25249357533473 19.082534654682387 30-31 26.948000913029475 26.348793765146567 27.8052039379105 18.898001383913456 32-33 23.25784099881319 26.183646913713826 31.639082063615355 18.91943002385763 34-35 20.995403395982976 28.759058740925475 28.918194747618088 21.327343115473465 36-37 23.775572342456535 29.78824861454965 27.707627648373666 18.728551394620148 38-39 25.18356770747309 26.908301644084233 27.41910254492036 20.489028103522315 40-41 23.466864930228382 26.21471162648497 27.082406158761042 23.2360172845256 42-43 26.070590815192222 26.073195871879424 28.24534657168708 19.610866741241274 44-45 23.68017003876935 27.91239871207951 28.914168621520535 19.4932626276306 46-47 24.58693447100422 28.523983970455074 27.536257234586703 19.352824323954003 48-49 21.642993426378176 28.492850022574174 29.159026140753596 20.705130410294057 50-51 20.582014253140564 26.98898907186664 30.365577818151568 22.063418856841217 52-53 25.764661110654362 26.314538400643567 27.36331200995441 20.55748847874766 54-55 26.670063993789594 26.263220306624156 27.60558878124209 19.461126918344156 56-57 27.35976964997302 26.06160203538565 27.02913511560111 19.54949319904022 58-59 25.968450988567888 26.29014646540666 26.910177426142727 20.831225119882728 60-61 22.814855718796554 26.381466316639695 26.652652375301923 24.15102558926183 62-63 25.259800692853656 26.327341295515883 27.042577664309874 21.370280347320584 64-65 23.138986414755063 27.439524922054066 28.70604255768961 20.71544610550126 66-67 21.64613838499797 27.672821912654992 27.687443795619306 22.993595906727737 68-69 21.231414295789037 26.631694232006247 31.76577536376698 20.371116108437732 70-71 21.003820720158693 29.874676843339397 29.09747882199011 20.0240236145118 72-73 20.59066352820339 33.029888336708666 26.424023326174773 19.955424808913175 74-75 20.7524150552943 33.25213459914001 26.050166248580602 19.945284096985088 76-77 20.642389710156483 33.00962849343317 26.089811096528614 20.258170699881735 78-79 20.642224511013634 32.662196239421824 26.484003630340442 20.2115756192241 80-81 20.42943629262626 32.53271546361112 26.607236690660756 20.430611553101862 82-83 20.134577017839046 32.44593812516057 26.722037406926745 20.697447450073632 84-85 20.28535618457984 32.06184987971766 26.754578949639228 20.898214986063262 86-87 20.175754721481855 31.846842203092297 27.262415487682816 20.714987587743032 88-89 20.28143150265101 31.77639048302759 27.321164197819257 20.621013816502142 90-91 20.60533434497646 31.34389373472638 27.021499521299152 21.029272398998007 92-93 20.485438326630472 31.549567037082078 26.81313299481657 21.15186164147088 94-95 20.449984145228733 31.45401120857061 26.742643313292707 21.35336133290795 96-97 20.68852725535714 31.635393296456048 26.600135791434766 21.07594365675204 98-99 20.92804489231242 31.249583515550338 26.63680088653147 21.18557070560577 100-101 21.02849643667046 31.373716601526358 26.156718502065917 21.441068459737266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3640.0 1 2208.5 2 683.0 3 783.0 4 907.5 5 798.5 6 833.0 7 1069.0 8 1322.5 9 1595.0 10 1866.5 11 2084.0 12 2194.5 13 2141.5 14 2050.5 15 1997.5 16 1937.0 17 1888.0 18 1920.5 19 1948.5 20 2082.0 21 2409.0 22 2946.5 23 3768.0 24 4934.0 25 6700.0 26 10008.0 27 14623.5 28 19142.0 29 23945.5 30 29641.5 31 35090.0 32 45517.0 33 65622.5 34 94730.0 35 126543.0 36 165008.0 37 213016.5 38 250749.5 39 261837.0 40 262889.0 41 262100.5 42 262534.0 43 270266.5 44 268579.5 45 258926.0 46 249272.0 47 228601.5 48 201695.0 49 183165.5 50 168186.0 51 156720.5 52 146921.5 53 131379.0 54 112741.0 55 96120.5 56 81847.0 57 70487.0 58 61245.0 59 52156.0 60 43234.5 61 35331.5 62 28849.5 63 23523.0 64 19351.5 65 15574.0 66 12124.0 67 9899.5 68 8731.0 69 6909.0 70 4838.0 71 3719.5 72 2820.5 73 2124.0 74 1632.0 75 1303.5 76 1053.5 77 830.5 78 622.0 79 459.5 80 353.0 81 272.5 82 233.0 83 200.5 84 159.0 85 125.5 86 109.0 87 91.0 88 77.5 89 58.5 90 45.0 91 41.5 92 32.0 93 21.5 94 16.0 95 16.0 96 14.0 97 10.5 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16138307758245862 2 0.15431082121103382 3 0.12059650316643325 4 0.11515183456267879 5 0.1279981523003628 6 0.14843987962050853 7 0.12462672049590275 8 0.11497745015899982 9 0.10800207401184109 10-11 0.09566922146276738 12-13 0.12471391269774222 14-15 0.1203349265609148 16-17 0.1100946868559887 18-19 0.1083217787519192 20-21 0.10208269230918277 22-23 0.11476431366561442 24-25 0.40230481928738004 26-27 0.29176448339976735 28-29 0.37781349859291163 30-31 0.21552943492477833 32-33 0.25542471127755567 34-35 0.26821290088068 36-37 0.2650352295247521 38-39 0.2844790905349571 40-41 0.27761028263449106 42-43 0.33268669012976526 44-45 0.27942194282826704 46-47 0.3054245950212865 48-49 0.3051920824830479 50-51 0.30536646688672686 52-53 0.3091447956331045 54-55 0.2869495362537425 56-57 0.2993889570495089 58-59 0.2996020935428943 60-61 0.27825938013707385 62-63 0.27479106810834775 64-65 0.28398500139120003 66-67 0.28320027157464467 68-69 0.28061356958674 70-71 0.25499843829078483 72-73 0.24476788660828536 74-75 0.2547659257525462 76-77 0.25007692289806727 78-79 0.24674424318331367 80-81 0.25609318482499166 82-83 0.2971122717792557 84-85 0.3026150685175698 86-87 0.2923942048574969 88-89 0.2988948679057518 90-91 0.2764864720330044 92-93 0.2607628116346174 94-95 0.2464536025105154 96-97 0.24560105653697373 98-99 0.26663375322514266 100-101 0.2671859705034594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5161012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.86114305378144 #Duplication Level Percentage of deduplicated Percentage of total 1 92.35891848467291 57.1342826867289 2 3.9718627328675793 4.914079374158092 3 1.028099507804715 1.9079823217748932 4 0.603942214765288 1.494422229752523 5 0.4183073872025174 1.2938486560094236 6 0.2016426750320364 0.748430781954238 7 0.23338237278838683 1.0106110244505342 8 0.14666987806314555 0.7258533046835941 9 0.11550642820896202 0.6430823711159342 >10 0.7311964214674586 9.062197647459193 >50 0.09380740167763943 4.048037343593518 >100 0.08513960148120657 11.09521596978077 >500 0.009601850249423142 3.9256630457491735 >1k 0.0018918294212013664 1.7160541612439975 >5k 0.0 0.0 >10k+ 3.121429749314071E-5 0.28023908154516897 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 14513 0.28120453895476316 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002964534862542463 0.0 0.0 2 0.0033520557596068367 0.0 0.0 3 0.003797704791230867 0.0 0.0 4 0.004320858002267772 0.0 0.0 5 0.004727754944185365 0.0 0.0 6 0.005580300917726988 0.0 0.0 7 0.006665359429507236 0.0 1.937604485321871E-5 8 0.0077116658515810465 0.0 1.937604485321871E-5 9 0.008602963914829106 1.937604485321871E-5 1.937604485321871E-5 10-11 0.012265036392087444 1.937604485321871E-5 1.937604485321871E-5 12-13 0.015985237003905434 1.937604485321871E-5 1.937604485321871E-5 14-15 0.020819560194783503 1.937604485321871E-5 3.875208970643742E-5 16-17 0.024995097860652137 1.937604485321871E-5 3.875208970643742E-5 18-19 0.029926301275796298 1.937604485321871E-5 3.875208970643742E-5 20-21 0.03501251304976621 1.937604485321871E-5 3.875208970643742E-5 22-23 0.04058312594506659 1.937604485321871E-5 5.812813455965613E-5 24-25 0.04606654663852748 1.937604485321871E-5 5.812813455965613E-5 26-27 0.052005304386039015 1.937604485321871E-5 5.812813455965613E-5 28-29 0.0583025189633351 1.937604485321871E-5 5.812813455965613E-5 30-31 0.08679499291999321 1.937604485321871E-5 7.750417941287484E-5 32-33 0.0946616671304 1.937604485321871E-5 7.750417941287484E-5 34-35 0.10316775082096302 1.937604485321871E-5 7.750417941287484E-5 36-37 0.11188697100491143 1.937604485321871E-5 7.750417941287484E-5 38-39 0.12145873716240148 1.937604485321871E-5 7.750417941287484E-5 40-41 0.1318442972037267 1.937604485321871E-5 7.750417941287484E-5 42-43 0.14296614694947424 1.937604485321871E-5 7.750417941287484E-5 44-45 0.15538619170038742 1.937604485321871E-5 7.750417941287484E-5 46-47 0.16885254287337442 1.937604485321871E-5 7.750417941287484E-5 48-49 0.1847505876754404 1.937604485321871E-5 7.750417941287484E-5 50-51 0.20025142355801537 1.937604485321871E-5 7.750417941287484E-5 52-53 0.21627541265162725 1.937604485321871E-5 7.750417941287484E-5 54-55 0.23428544634269405 1.937604485321871E-5 7.750417941287484E-5 56-57 0.25442684496761486 1.937604485321871E-5 7.750417941287484E-5 58-59 0.274907324377467 1.937604485321871E-5 7.750417941287484E-5 60-61 0.2972769681605081 1.937604485321871E-5 7.750417941287484E-5 62-63 0.32130326377849927 1.937604485321871E-5 8.719220183948419E-5 64-65 0.34770312489100974 1.937604485321871E-5 9.688022426609355E-5 66-67 0.37598246235428245 1.937604485321871E-5 9.688022426609355E-5 68-69 0.4062672204598633 1.937604485321871E-5 9.688022426609355E-5 70-71 0.4403787474239549 1.937604485321871E-5 9.688022426609355E-5 72-73 0.47591441368475795 1.937604485321871E-5 9.688022426609355E-5 74-75 0.5154318571628975 1.937604485321871E-5 9.688022426609355E-5 76-77 0.5578653953914465 1.937604485321871E-5 9.688022426609355E-5 78-79 0.6059858027844152 1.937604485321871E-5 9.688022426609355E-5 80-81 0.6582526837759726 1.937604485321871E-5 9.688022426609355E-5 82-83 0.7169911637485051 1.937604485321871E-5 9.688022426609355E-5 84-85 0.7802733262391175 1.937604485321871E-5 1.0656824669270291E-4 86-87 0.8493683021856954 1.937604485321871E-5 1.1625626911931226E-4 88-89 0.9271824983162217 1.937604485321871E-5 1.3563231397253098E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5820 0.0 80.60431 9 ATCGGGA 9340 0.0 75.53607 2 TCGGGAG 8185 0.0 74.707726 3 CGGGAGA 8190 0.0 73.56159 4 AGAGCGG 6550 0.0 72.84919 8 ATCCGGA 1610 0.0 71.904205 2 ATTCGGA 1300 0.0 71.53243 2 GATCGGG 10050 0.0 71.52918 1 GATCCGG 1795 0.0 69.25857 1 AAGCGGC 1505 0.0 66.18625 9 GAGAGCG 7860 0.0 66.01808 7 TCGGAGA 1795 0.0 65.01326 4 CGGAAAG 2675 0.0 62.423798 5 CGGAGAG 3325 0.0 60.921074 5 GTTCGGA 970 0.0 60.162197 2 GAGCGTC 14210 0.0 59.951107 9 AGAGCGT 14355 0.0 59.772785 8 AATCGGA 1120 0.0 59.729847 2 ATCGGAG 1385 0.0 59.25536 3 TCGGGAA 2350 0.0 59.14678 3 >>END_MODULE