FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5161012
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT145130.28120453895476316Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC58200.080.604319
ATCGGGA93400.075.536072
TCGGGAG81850.074.7077263
CGGGAGA81900.073.561594
AGAGCGG65500.072.849198
ATCCGGA16100.071.9042052
ATTCGGA13000.071.532432
GATCGGG100500.071.529181
GATCCGG17950.069.258571
AAGCGGC15050.066.186259
GAGAGCG78600.066.018087
TCGGAGA17950.065.013264
CGGAAAG26750.062.4237985
CGGAGAG33250.060.9210745
GTTCGGA9700.060.1621972
GAGCGTC142100.059.9511079
AGAGCGT143550.059.7727858
AATCGGA11200.059.7298472
ATCGGAG13850.059.255363
TCGGGAA23500.059.146783
Produced by FastQC (version 0.11.2)