##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3488542 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.936731161614222 33.0 33.0 33.0 27.0 33.0 2 31.83437063392099 33.0 33.0 33.0 27.0 33.0 3 31.764677048463227 33.0 33.0 33.0 27.0 33.0 4 35.46547640819575 37.0 37.0 37.0 33.0 37.0 5 35.47586756874362 37.0 37.0 37.0 33.0 37.0 6 35.6300207937872 37.0 37.0 37.0 33.0 37.0 7 35.41478531718982 37.0 37.0 37.0 33.0 37.0 8 35.46487128433598 37.0 37.0 37.0 33.0 37.0 9 35.324454743557624 37.0 37.0 37.0 33.0 37.0 10-11 35.275125109573 37.0 37.0 37.0 33.0 37.0 12-13 35.24260507684872 37.0 37.0 37.0 33.0 37.0 14-15 36.92356047311456 40.0 37.0 40.0 33.0 40.0 16-17 36.886857747448644 40.0 37.0 40.0 33.0 40.0 18-19 36.88889140506263 40.0 37.0 40.0 33.0 40.0 20-21 36.85491789979883 40.0 37.0 40.0 33.0 40.0 22-23 37.02016616110684 40.0 37.0 40.0 33.0 40.0 24-25 36.65212845366345 40.0 37.0 40.0 33.0 40.0 26-27 36.579973954735244 40.0 37.0 40.0 33.0 40.0 28-29 36.40401677835611 40.0 37.0 40.0 33.0 40.0 30-31 36.41781036318324 40.0 37.0 40.0 33.0 40.0 32-33 36.29171470488244 40.0 37.0 40.0 33.0 40.0 34-35 36.31353872190732 40.0 37.0 40.0 33.0 40.0 36-37 36.14898086363874 40.0 37.0 40.0 33.0 40.0 38-39 36.08904622618847 40.0 37.0 40.0 33.0 40.0 40-41 36.15767145701557 37.0 37.0 40.0 27.0 40.0 42-43 35.90780876366115 37.0 37.0 40.0 27.0 40.0 44-45 35.69833242655528 37.0 37.0 40.0 27.0 40.0 46-47 35.56096314735497 37.0 37.0 40.0 27.0 40.0 48-49 35.43648750681517 37.0 37.0 40.0 27.0 40.0 50-51 34.38434337324877 37.0 35.0 38.5 24.5 38.5 52-53 34.81487939660752 37.0 35.0 38.5 27.0 40.0 54-55 35.46102899148126 37.0 37.0 40.0 27.0 40.0 56-57 35.55893651273226 37.0 37.0 40.0 27.0 40.0 58-59 35.32238009460686 37.0 37.0 40.0 27.0 40.0 60-61 35.17562236601996 37.0 35.0 40.0 27.0 40.0 62-63 34.91403213720804 37.0 33.0 40.0 27.0 40.0 64-65 34.70163738891491 37.0 33.0 40.0 27.0 40.0 66-67 34.51330197543845 37.0 33.0 40.0 27.0 40.0 68-69 34.36799757606473 37.0 33.0 40.0 27.0 40.0 70-71 34.04863665107085 37.0 33.0 37.0 27.0 40.0 72-73 33.93172520210449 37.0 33.0 37.0 27.0 40.0 74-75 33.74648950765105 37.0 33.0 37.0 27.0 40.0 76-77 33.43108396000392 37.0 33.0 37.0 24.5 40.0 78-79 33.033924917630344 37.0 33.0 37.0 22.0 37.0 80-81 32.62172334459496 37.0 33.0 37.0 22.0 37.0 82-83 32.292155576742374 37.0 33.0 37.0 22.0 37.0 84-85 32.03095046583931 37.0 33.0 37.0 22.0 37.0 86-87 31.837903628507267 35.0 33.0 37.0 15.0 37.0 88-89 31.62491966557949 33.0 33.0 37.0 15.0 37.0 90-91 31.432019021126877 33.0 33.0 37.0 15.0 37.0 92-93 31.252819229351402 33.0 33.0 37.0 15.0 37.0 94-95 31.058561570994414 33.0 33.0 37.0 15.0 37.0 96-97 30.893933626139514 33.0 30.0 37.0 15.0 37.0 98-99 30.70350851444529 33.0 27.0 37.0 15.0 37.0 100-101 29.319747619492613 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.367032007362905 2306 2 0.31481185363072584 2306 3 0.27319427162640864 2306 4 0.38339620120036955 2306 5 0.44676626538168307 2306 6 0.39733651595182806 2306 7 0.5420801573378142 2306 8 0.3477831431540963 2306 9 0.37039213478588806 2306 10-11 0.3597549408489158 2306 12-13 0.36445636184881636 2306 14-15 0.6253415532281537 2306 16-17 0.6267481216597801 2306 18-19 0.6380864909392514 2306 20-21 0.5572372821360077 2306 22-23 0.6189696076319962 2306 24-25 0.8587405323559025 2306 26-27 0.656056703610183 2306 28-29 0.7899044611821822 2306 30-31 0.5950135190007941 2306 32-33 0.6309175443772972 2306 34-35 0.6346486144034316 2306 36-37 0.637332667414718 2306 38-39 0.5784692957654585 2306 40-41 0.49807478415154094 2306 42-43 0.684762638975144 2306 44-45 0.794893089953888 2306 46-47 0.668278945494464 2306 48-49 0.8662430333614495 2306 50-51 0.6503911676024998 2306 52-53 0.7000501361078832 2306 54-55 0.8128240951728287 2306 56-57 0.846452633904434 2306 58-59 1.0348137454131106 2306 60-61 1.0505491939456633 2306 62-63 0.8854798391088821 2306 64-65 0.8075648777950377 2306 66-67 0.8692450504193872 2306 68-69 0.7238839310747096 2306 70-71 0.8766021232222059 2306 72-73 0.7720357872239205 2306 74-75 0.6522304020781817 2306 76-77 0.8963416791763166 2306 78-79 0.8487170398872266 2306 80-81 0.7245411075667292 2306 82-83 0.7650173987840319 2306 84-85 0.7216462518999691 2306 86-87 0.8918219060365473 2306 88-89 0.8524784688599318 2306 90-91 0.5930116331053163 2306 92-93 0.573602707848579 2306 94-95 0.6362309071625987 2306 96-97 0.7345276634038171 2306 98-99 0.7604045560511459 2306 100-101 0.6508890745492479 2307 1 0.16361328993703594 2307 2 0.14146086698520222 2307 3 0.10712636043881574 2307 4 0.16541393488630973 2307 5 0.242875279933088 2307 6 0.19581684505629227 2307 7 0.33098112156253023 2307 8 0.28222592326454077 2307 9 0.22896802769336233 2307 10-11 0.2049431845409444 2307 12-13 0.22266601936126307 2307 14-15 0.45701031163595474 2307 16-17 0.3207095526709196 2307 18-19 0.3136782570828771 2307 20-21 0.29431561270974527 2307 22-23 0.2683715315712618 2307 24-25 0.37060057449141226 2307 26-27 0.3946407408642827 2307 28-29 0.3632044480853054 2307 30-31 0.3460627866601058 2307 32-33 0.3481246232803201 2307 34-35 0.17071206745026757 2307 36-37 0.3161418407765453 2307 38-39 0.102456521544255 2307 40-41 -0.048751739530814575 2307 42-43 0.19012606851700298 2307 44-45 0.07451890970101971 2307 46-47 0.01710243868192407 2307 48-49 0.1049055651170292 2307 50-51 0.11600558873373501 2307 52-53 0.14739919031418225 2307 54-55 0.2149312605498963 2307 56-57 0.3382523436916145 2307 58-59 0.25193625841674105 2307 60-61 0.1247915485378499 2307 62-63 0.25909566880443435 2307 64-65 0.21512762786073836 2307 66-67 0.205057537897126 2307 68-69 0.25359973761221966 2307 70-71 0.18749520850713708 2307 72-73 0.1634592438095197 2307 74-75 0.3510868976499566 2307 76-77 0.18456243000007078 2307 78-79 0.2636970866716908 2307 80-81 0.18155799559367125 2307 82-83 0.24244567605956746 2307 84-85 0.37320547361615297 2307 86-87 0.37178130573141743 2307 88-89 0.2570133260563203 2307 90-91 0.352839925299147 2307 92-93 0.3962677999993929 2307 94-95 0.28007229796373423 2307 96-97 0.45801061621951433 2307 98-99 0.3615139305497159 2307 100-101 0.4746472351585389 2201 1 0.2725596090143334 2201 2 0.29402731453825126 2201 3 0.2510211494876984 2201 4 0.35513971367510067 2201 5 0.3076580075473032 2201 6 0.2815802918070105 2201 7 0.4374840342154016 2201 8 0.29593045276927654 2201 9 0.2829533371723798 2201 10-11 0.33066926969561905 2201 12-13 0.3382986550063336 2201 14-15 0.5760718504080629 2201 16-17 0.5752478446455243 2201 18-19 0.47474526927537397 2201 20-21 0.44965370170705654 2201 22-23 0.5392784299888049 2201 24-25 0.6929558631224992 2201 26-27 0.5326761835174523 2201 28-29 0.5085835679444273 2201 30-31 0.622247470661911 2201 32-33 0.37179673104194677 2201 34-35 0.5341004938656653 2201 36-37 0.5830640036563253 2201 38-39 0.3813078553243443 2201 40-41 0.3253972936156728 2201 42-43 0.5260884155962913 2201 44-45 0.448236596314068 2201 46-47 0.3662677104074774 2201 48-49 0.43655889985421226 2201 50-51 0.333237105945436 2201 52-53 0.4607108471400707 2201 54-55 0.629336334823833 2201 56-57 0.5723098391465911 2201 58-59 0.6035342493848859 2201 60-61 0.48746244943957606 2201 62-63 0.5062478317119172 2201 64-65 0.4321765723827795 2201 66-67 0.47730963585029684 2201 68-69 0.38340644607499996 2201 70-71 0.4213003677243776 2201 72-73 0.4099957062969253 2201 74-75 0.32062852837933065 2201 76-77 0.37904440052641064 2201 78-79 0.5168470345617848 2201 80-81 0.5829781439396768 2201 82-83 0.45147082299842367 2201 84-85 0.42461972422105987 2201 86-87 0.4462061473335268 2201 88-89 0.41715282940318943 2201 90-91 0.6675421032280617 2201 92-93 0.6623379569940937 2201 94-95 0.5607820139182351 2201 96-97 0.701860637851162 2201 98-99 0.4341199721323541 2201 100-101 0.5327464169558134 1101 1 0.3841420947198877 1101 2 0.2919882150469384 1101 3 0.3650166828547512 1101 4 0.437535821895807 1101 5 0.4739052418330729 1101 6 0.430993956813829 1101 7 0.44563596125563265 1101 8 0.4553264415268714 1101 9 0.41548765062785975 1101 10-11 0.45168433420927556 1101 12-13 0.48789983060925124 1101 14-15 0.6911234162690789 1101 16-17 0.5957186008613604 1101 18-19 0.6001368328902004 1101 20-21 0.5979124373580689 1101 22-23 0.5070633584647339 1101 24-25 0.7961949767777057 1101 26-27 0.7738616653865975 1101 28-29 0.7026675862830913 1101 30-31 0.7856494082535193 1101 32-33 0.6564935236360867 1101 34-35 0.7347272639048867 1101 36-37 0.6768811793050205 1101 38-39 0.6642113598857549 1101 40-41 0.6349901640282383 1101 42-43 0.586583608100014 1101 44-45 0.5165001104897513 1101 46-47 0.5777853855836881 1101 48-49 0.6326500952794163 1101 50-51 0.5199246941567921 1101 52-53 0.7334320881830152 1101 54-55 0.9019909590837187 1101 56-57 0.8981179499053482 1101 58-59 0.8824901953932809 1101 60-61 0.8235301707199625 1101 62-63 0.8420887876743777 1101 64-65 0.8561547514072103 1101 66-67 0.8036689222477378 1101 68-69 0.7120341904182084 1101 70-71 0.713314276235657 1101 72-73 0.8245896819221556 1101 74-75 0.6899466980040856 1101 76-77 0.7519177603228542 1101 78-79 0.8788634682515237 1101 80-81 0.7971612057865158 1101 82-83 0.672329333600949 1101 84-85 0.7908964129609828 1101 86-87 0.8259199330195628 1101 88-89 0.8853384421810269 1101 90-91 0.8349338943235516 1101 92-93 0.6995216569313527 1101 94-95 0.8190065487810756 1101 96-97 0.7633975558887336 1101 98-99 0.7026583051282778 1101 100-101 0.8225730595200211 2304 1 0.240718054138501 2304 2 0.16702260868113328 2304 3 0.18573585633176037 2304 4 0.23947279551804712 2304 5 0.262667643367287 2304 6 0.2637009065001692 2304 7 0.26504765943571584 2304 8 0.26865262144401925 2304 9 0.18280842272788078 2304 10-11 0.22475186020534466 2304 12-13 0.19817449351715766 2304 14-15 0.2359368562324491 2304 16-17 0.3212753294974746 2304 18-19 0.3090115283470638 2304 20-21 0.2409575445662142 2304 22-23 0.30993137379860514 2304 24-25 0.3478027541869366 2304 26-27 0.35625243499718096 2304 28-29 0.31594495999552663 2304 30-31 0.16708843101227444 2304 32-33 0.23699712574431686 2304 34-35 0.23106817774460353 2304 36-37 0.259833453008703 2304 38-39 0.22836022863689465 2304 40-41 0.2599287899241105 2304 42-43 0.24171631063628496 2304 44-45 0.19455879930443132 2304 46-47 0.15919054914567 2304 48-49 0.2115623668591411 2304 50-51 0.1639390294924894 2304 52-53 0.25462253202812946 2304 54-55 0.3655455161799068 2304 56-57 0.4405453853711734 2304 58-59 0.5013031340198708 2304 60-61 0.32244655574385206 2304 62-63 0.3393270391882126 2304 64-65 0.3363826355246218 2304 66-67 0.4222085219920402 2304 68-69 0.557764259522699 2304 70-71 0.3990511930351275 2304 72-73 0.4270021152122965 2304 74-75 0.5053186526589641 2304 76-77 0.5288273741969789 2304 78-79 0.6036663815216414 2304 80-81 0.40451899543608505 2304 82-83 0.38178484185784356 2304 84-85 0.452546062680355 2304 86-87 0.5095825430188512 2304 88-89 0.5071784516147098 2304 90-91 0.3454533468113752 2304 92-93 0.3513049022146859 2304 94-95 0.28221139416315566 2304 96-97 0.24065310593517708 2304 98-99 0.3468280583147312 2304 100-101 0.4736928266018481 2202 1 0.18729236354266376 2202 2 0.15343687044527954 2202 3 0.11703056725546901 2202 4 0.15487040961349408 2202 5 0.17431874967459748 2202 6 0.27426948522222006 2202 7 0.1743703663030871 2202 8 0.20394635453291698 2202 9 0.1746647566095163 2202 10-11 0.2556122928755684 2202 12-13 0.28056195653464755 2202 14-15 0.35687195569987296 2202 16-17 0.29479927452184995 2202 18-19 0.2196404161777039 2202 20-21 0.2788199840286083 2202 22-23 0.3521812892914937 2202 24-25 0.349458195066191 2202 26-27 0.3524089492949187 2202 28-29 0.3221917474084748 2202 30-31 0.3004139307196425 2202 32-33 0.2577857203980187 2202 34-35 0.3326337204073013 2202 36-37 0.26768858659499983 2202 38-39 0.2716868158601642 2202 40-41 0.3778722136906367 2202 42-43 0.19284207549922883 2202 44-45 0.26168672556651273 2202 46-47 0.3429291499805558 2202 48-49 0.2985208823100791 2202 50-51 0.1735737697698312 2202 52-53 0.4136692546238905 2202 54-55 0.48600447887562837 2202 56-57 0.46414781124425986 2202 58-59 0.49769179113601325 2202 60-61 0.5285712532300764 2202 62-63 0.41733980681085114 2202 64-65 0.4897552206428841 2202 66-67 0.47715046072615763 2202 68-69 0.5355688654469049 2202 70-71 0.48980191672894335 2202 72-73 0.5844043960212986 2202 74-75 0.5121195774502638 2202 76-77 0.6567920556462212 2202 78-79 0.39463203677785685 2202 80-81 0.4724453628634038 2202 82-83 0.5138129134008906 2202 84-85 0.42979544680863313 2202 86-87 0.33437071753819225 2202 88-89 0.34345812166671763 2202 90-91 0.2715346428081098 2202 92-93 0.24658378188120622 2202 94-95 0.35002908745826744 2202 96-97 0.3716763096826803 2202 98-99 0.3036992998396215 2202 100-101 0.34462335602379923 1102 1 0.09446290524779855 1102 2 0.06257553055290188 1102 3 0.07957971019651211 1102 4 -0.017888208549045714 1102 5 -0.01211030569680105 1102 6 0.10401801943142175 1102 7 0.11212887453605447 1102 8 0.09594800524547509 1102 9 0.059088848218507906 1102 10-11 0.042333824085829974 1102 12-13 0.03546633170633129 1102 14-15 0.0039000413155534375 1102 16-17 0.0398899082948887 1102 18-19 0.09604577730392805 1102 20-21 0.08074445912753703 1102 22-23 0.09433042252840806 1102 24-25 -0.00595838873210397 1102 26-27 0.09781395637210011 1102 28-29 -0.04223040590208882 1102 30-31 0.08300590070558655 1102 32-33 0.02422316074227382 1102 34-35 0.03535073717648629 1102 36-37 -0.008739746021049655 1102 38-39 -0.07195548948678265 1102 40-41 0.0611033293696579 1102 42-43 0.045332763706369406 1102 44-45 0.010017754590698758 1102 46-47 0.04663407980378764 1102 48-49 -0.02449402338172746 1102 50-51 -0.021974385826759146 1102 52-53 -0.01676364802981567 1102 54-55 0.1594924228868564 1102 56-57 0.21367650399970017 1102 58-59 0.23924187138459985 1102 60-61 0.15444705707427886 1102 62-63 0.1918362851475237 1102 64-65 0.1238318968982739 1102 66-67 0.19356533391924557 1102 68-69 0.2153299973323186 1102 70-71 0.2628807458349556 1102 72-73 0.2310090000739322 1102 74-75 0.2543493257064924 1102 76-77 0.21765323633026412 1102 78-79 0.10535072599280682 1102 80-81 0.13943381570955893 1102 82-83 0.06408819221135076 1102 84-85 0.03712827994135637 1102 86-87 0.0490838935955864 1102 88-89 0.08021577800868229 1102 90-91 0.09308521079019627 1102 92-93 0.1832940421514735 1102 94-95 0.19721137772843633 1102 96-97 0.14956752691639963 1102 98-99 0.22834580045282848 1102 100-101 0.062121983167710226 2305 1 0.24141591129740547 2305 2 0.2334525418856792 2305 3 0.23306064253957004 2305 4 0.27266032555301933 2305 5 0.23196334862134194 2305 6 0.23219107413824247 2305 7 0.2687770958899165 2305 8 0.3548067997168971 2305 9 0.29404259311968417 2305 10-11 0.2503787760847018 2305 12-13 0.2652746536434236 2305 14-15 0.23582297833898735 2305 16-17 0.2820352927156975 2305 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64-65 -0.8613650406052713 1213 66-67 -0.6986707597118809 1213 68-69 -0.5578581776964882 1213 70-71 -0.6231621887937919 1213 72-73 -0.6961953368281755 1213 74-75 -0.7143752873066092 1213 76-77 -0.9196655799451747 1213 78-79 -0.8302945260986476 1213 80-81 -0.8329464927310752 1213 82-83 -0.7229502421793654 1213 84-85 -0.6557864611475139 1213 86-87 -0.982769680723397 1213 88-89 -0.7742004445739887 1213 90-91 -0.6883084864216684 1213 92-93 -0.6198775433626551 1213 94-95 -0.6591971393312193 1213 96-97 -0.6494865681823754 1213 98-99 -0.8454757907351578 1213 100-101 -1.1630773797644842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1714.0 3 125.0 4 157.0 5 182.0 6 380.0 7 1519.0 8 6182.0 9 14585.0 10 23476.0 11 20737.0 12 14641.0 13 11421.0 14 9931.0 15 9202.0 16 9186.0 17 9903.0 18 10801.0 19 11808.0 20 13524.0 21 15562.0 22 17550.0 23 19672.0 24 22470.0 25 26647.0 26 32192.0 27 39908.0 28 49739.0 29 57926.0 30 64297.0 31 81811.0 32 107500.0 33 147490.0 34 216094.0 35 337849.0 36 576780.0 37 970823.0 38 531397.0 39 3361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.67614527977414 28.935038622261615 16.17546431852045 18.213351779443784 2 31.78008610180042 26.888235912271945 27.065188491966648 14.266489493960988 3 35.13743756425837 23.411860336821487 23.67414642211125 17.77655567680889 4 30.284552787192588 23.638635209715716 26.978915201874536 19.09789680121716 5 33.57606069791581 21.471210284108132 26.819421863374576 18.133307154601486 6 31.61922824932918 21.793679203408974 28.42150625803536 18.16558628922649 7 33.86470021596437 22.945755169195053 22.998282388627945 20.191262226212633 8 29.35398305269599 28.551706956981953 22.61179859319697 19.482511397125087 9 30.058405149961843 24.157478950067627 25.071912291541885 20.712203608428638 10-11 26.04219543921037 29.80611378472538 27.749944833091767 16.401745942972482 12-13 26.51058796885717 28.105259547283946 27.25332233411364 18.130830149745247 14-15 24.86894844030454 28.14960378492777 28.179081147275014 18.802366627492678 16-17 25.558038228702078 27.996770723213643 28.29167326159108 18.153517786493204 18-19 24.988502425288726 28.014104456955017 28.67962210754383 18.31777101021243 20-21 24.393906305107745 29.164541872342454 27.939957258273495 18.501594564276306 22-23 25.543798651854633 28.620478653025632 27.38020615384792 18.455516541271813 24-25 23.313488736537323 30.32093956391207 27.67786552873643 18.68770617081418 26-27 23.24855997429411 30.1803365774796 27.72578373843828 18.845319709788004 28-29 23.56780532232734 29.91069983331307 27.60180168643721 18.919693157922378 30-31 24.11510402399837 28.483768653741766 28.356976859599815 19.044150462660053 32-33 22.71625843369417 29.609654297751486 28.58250136978152 19.091585898772824 34-35 24.40305562398016 28.58509387360441 27.895963114524392 19.11588738789104 36-37 22.822290488117115 29.129045535101973 28.709840693537547 19.33882328324336 38-39 22.78259544662018 29.97241639032748 27.90897840026563 19.336009762786706 40-41 24.285013710293832 28.41087935315124 27.849431394345075 19.454675542209856 42-43 23.880042175840813 28.420977246214413 28.15798223230536 19.540998345639412 44-45 22.534613658149137 29.209231688685993 28.582709635653043 19.673445017511828 46-47 23.177089847911468 29.29466150359842 27.766257008428084 19.76199164006203 48-49 21.722598802801553 28.982634354155202 28.78276581710318 20.512001025940066 50-51 21.390277920151384 28.34343590894498 29.386109125069172 20.88017704583446 52-53 23.714013690885952 28.290850524579586 27.908508121307484 20.08662766322698 54-55 23.40548998908695 28.302031621398783 28.499593491727815 19.792884897786458 56-57 23.6313288716689 28.20312366347379 28.324141507208385 19.84140595764893 58-59 23.091480581854317 28.45737645716599 27.877529051312482 20.57361390966721 60-61 21.749412823073076 28.33325404175068 27.85553543162337 22.06179770355287 62-63 22.869154405476607 28.364677999134756 28.24808590330847 20.51808169208016 64-65 21.675769867143917 29.13631687560739 28.989329857927405 20.198583399321297 66-67 21.398538354818363 28.980456202102832 28.216631316647817 21.404374126430987 68-69 21.21452897374814 28.372782077179963 30.18154461689006 20.231144332181834 70-71 21.166949390616725 29.956208323364354 28.686127558436205 20.19071472758272 72-73 21.1260271724848 30.991021099718797 27.74330900155911 20.139642726237295 74-75 20.970219761191927 31.09351795371414 27.8987433082656 20.03751897682833 76-77 21.106441482244424 30.990207190513193 27.76308620063362 20.14026512660876 78-79 21.03532570158056 31.099327878714988 27.753205030111616 20.112141389592832 80-81 21.08821435217484 30.85219675485683 27.799782009379204 20.25980688358913 82-83 21.271256845181064 30.791997494023654 27.606509385695087 20.330236275100194 84-85 21.192264629111758 30.556365432644007 27.844063102555744 20.407306835688495 86-87 21.484470852364097 30.175063634739256 27.763074500549774 20.577391012346872 88-89 21.496542347988886 30.268137488371583 27.708383859105655 20.526936304533873 90-91 21.548139255150147 30.03781323404263 27.745433529899678 20.668613980907544 92-93 21.633643574788618 29.972928719383855 27.64925058912154 20.744177116705988 94-95 21.55720414326802 29.905352686286207 27.82567226663153 20.71177090381424 96-97 21.719479378960667 29.72239560164191 27.711788229587302 20.84633678981012 98-99 21.678825182808886 29.784673471948224 27.7196347400766 20.81686660516629 100-101 21.91960109676545 29.681822186204375 27.623252818692666 20.775323898337504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1365.0 1 799.0 2 186.5 3 129.5 4 138.0 5 171.0 6 235.0 7 330.0 8 475.0 9 672.5 10 873.0 11 1021.5 12 1115.5 13 1144.5 14 1089.5 15 1044.5 16 1005.0 17 1014.5 18 1174.0 19 1493.0 20 2006.5 21 2922.5 22 4216.5 23 6044.5 24 8574.0 25 11732.5 26 15701.0 27 20759.5 28 26609.5 29 33272.0 30 40982.0 31 49680.0 32 59766.0 33 71025.5 34 83042.5 35 95646.0 36 108909.5 37 122731.5 38 134898.5 39 145186.0 40 154820.5 41 162032.0 42 165509.5 43 166725.0 44 166595.5 45 164660.5 46 160699.0 47 155580.5 48 149508.0 49 140886.5 50 130908.0 51 119802.5 52 106191.5 53 91372.5 54 76842.5 55 63456.5 56 52169.0 57 42724.5 58 34614.0 59 27862.0 60 22045.0 61 17404.5 62 13637.5 63 10348.0 64 7912.5 65 6056.0 66 4526.5 67 3384.5 68 2566.0 69 1960.0 70 1474.5 71 1154.5 72 928.5 73 720.5 74 572.5 75 458.0 76 370.0 77 278.0 78 217.0 79 185.5 80 148.5 81 126.5 82 116.5 83 98.5 84 75.5 85 58.5 86 48.5 87 39.0 88 35.5 89 33.0 90 27.0 91 19.0 92 16.0 93 10.5 94 9.0 95 9.5 96 5.5 97 4.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1709883383946646 2 0.16304805847256532 3 0.13071363337463043 4 0.1253532277954515 5 0.13753596774813087 6 0.1576589876229095 7 0.13283486338992048 8 0.12412062116494513 9 0.117212290980014 10-11 0.10500088575685775 12-13 0.13353716251660436 14-15 0.12935203302697804 16-17 0.11954851052388075 18-19 0.11785725956574408 20-21 0.11153656742558926 22-23 0.1246365960335292 24-25 0.4194445702531315 26-27 0.3078506722865885 28-29 0.38596353433612096 30-31 0.21895966853774443 32-33 0.2571991393539192 34-35 0.27085814073615855 36-37 0.2676189651722697 38-39 0.2874266670718025 40-41 0.2800310272887642 42-43 0.3345237064653371 44-45 0.2835998534631373 46-47 0.30858163668374927 48-49 0.3083236492494572 50-51 0.3095419232447251 52-53 0.31245144819812976 54-55 0.2906085120947376 56-57 0.3022036140026406 58-59 0.30264792569503246 60-61 0.28150728871832414 62-63 0.27940039133827255 64-65 0.28675303321559553 66-67 0.28653804368701885 68-69 0.28358552082789884 70-71 0.2579731016567953 72-73 0.24828424023560558 74-75 0.25711314354248854 76-77 0.2535729826385923 78-79 0.2504914660623263 80-81 0.2589763861234865 82-83 0.2997384007416279 84-85 0.3052278000379528 86-87 0.29503729638341747 88-89 0.3018882960273948 90-91 0.2790134101868345 92-93 0.2639784758217043 94-95 0.2502048133575574 96-97 0.24933052260801217 98-99 0.270327833232336 100-101 0.269525205658983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3488542.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.22086657340883 #Duplication Level Percentage of deduplicated Percentage of total 1 97.96946883493611 93.28737720199186 2 1.435287856548868 2.7333870696574745 3 0.29174256926747183 0.8333994078600425 4 0.09154698234385064 0.3486873196384806 5 0.06808459880714482 0.32415372493596034 6 0.043694184489225456 0.2496358866769468 7 0.011248568691605119 0.07497689209676084 8 0.019707624687782574 0.15012616806993298 9 0.005846006356285676 0.05009956121152605 >10 0.05922119949037976 0.9784379651694758 >50 0.00364463492124458 0.2277062373387359 >100 4.7693596325925177E-4 0.0753983299992782 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.0003496796704622E-5 0.6666142353535482 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 23333 0.668846756037336 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0043284558420107885 0.0 0.0 2 0.005532397202040279 0.0 0.0 3 0.008284263167821973 0.0 0.0 4 0.011265451297418808 0.0 0.0 5 0.013988651992723608 0.0 0.0 6 0.023820839766297782 0.0 0.0 7 0.033079722130334104 0.0 2.866527047689264E-5 8 0.04193729070769393 0.0 5.733054095378528E-5 9 0.046323077090658506 0.0 8.599581143067792E-5 10-11 0.05624126067566336 0.0 8.599581143067792E-5 12-13 0.06852432907501185 0.0 1.433263523844632E-4 14-15 0.08064973848673744 0.0 1.433263523844632E-4 16-17 0.09405075243468475 0.0 1.433263523844632E-4 18-19 0.11180888749511975 0.0 1.433263523844632E-4 20-21 0.1321898948041904 0.0 1.7199162286135584E-4 22-23 0.15572408186571926 0.0 1.7199162286135584E-4 24-25 0.17740935898148852 0.0 1.7199162286135584E-4 26-27 0.19847833278200464 0.0 1.7199162286135584E-4 28-29 0.22231350518354087 0.0 2.0065689333824847E-4 30-31 0.2533579931100156 0.0 2.0065689333824847E-4 32-33 0.28827229255087083 0.0 2.149895285766948E-4 34-35 0.3290486398042506 0.0 3.009853400073727E-4 36-37 0.37603101811587764 0.0 3.15317975245819E-4 38-39 0.4320859545334412 0.0 3.15317975245819E-4 40-41 0.4959665097911964 0.0 3.15317975245819E-4 42-43 0.5657234454967146 0.0 3.4398324572271167E-4 44-45 0.6397371738680515 0.0 3.4398324572271167E-4 46-47 0.71862399822046 0.0 3.7264851619960433E-4 48-49 0.8043044916758921 0.0 4.0131378667649693E-4 50-51 0.8933244891418822 0.0 4.5864432763028225E-4 52-53 0.9873609089413284 0.0 4.5864432763028225E-4 54-55 1.0852671402551555 0.0 4.5864432763028225E-4 56-57 1.18651287557954 0.0 4.5864432763028225E-4 58-59 1.2904674789639912 0.0 4.5864432763028225E-4 60-61 1.401932383213388 0.0 4.5864432763028225E-4 62-63 1.5166364630266742 0.0 5.589727742994065E-4 64-65 1.6332467833266735 0.0 5.733054095378528E-4 66-67 1.7550168523125134 0.0 5.876380447762991E-4 68-69 1.8794814567231812 0.0 6.019706800147454E-4 70-71 2.0098941047578043 0.0 6.019706800147454E-4 72-73 2.140622070767673 0.0 6.30635950491638E-4 74-75 2.2800069484615637 0.0 6.593012209685307E-4 76-77 2.4237346146327035 0.0 6.593012209685307E-4 78-79 2.5727080253011145 0.0 6.879664914454233E-4 80-81 2.7290770757525635 0.0 6.879664914454233E-4 82-83 2.8947050085680495 0.0 7.16631761922316E-4 84-85 3.068445212928496 0.0 7.16631761922316E-4 86-87 3.2500826993053256 0.0 7.452970323992087E-4 88-89 3.439775126686163 0.0 7.452970323992087E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1905 0.0 76.44612 2 GATCGGG 2145 0.0 69.6709 1 GAGCGGC 1220 0.0 67.64262 9 TCGGGAG 2120 0.0 60.854855 3 AGAGCGG 1675 0.0 52.381954 8 AAGCGGC 445 0.0 52.223633 9 CGGGAGA 2555 0.0 50.308407 4 GAGAGCG 2415 0.0 50.078094 7 GATCCGG 370 0.0 46.16034 1 AGCGGCG 1185 0.0 40.823746 10-11 TCGGAGA 615 0.0 39.333015 4 CGTCGGG 615 0.0 39.330193 12-13 CGCCGGA 935 0.0 38.866604 58-59 GTTCGGA 220 0.0 38.81163 2 GGCGCCG 1560 0.0 38.36528 56-57 GCGTCGG 610 0.0 38.097565 10-11 TTCGGAG 400 0.0 37.94479 3 AAGCGTC 305 0.0 37.320065 9 TCGGGAA 740 0.0 36.53468 3 AAAGCGG 650 0.0 36.482235 8 >>END_MODULE