FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488542
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT233330.668846756037336Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA19050.076.446122
GATCGGG21450.069.67091
GAGCGGC12200.067.642629
TCGGGAG21200.060.8548553
AGAGCGG16750.052.3819548
AAGCGGC4450.052.2236339
CGGGAGA25550.050.3084074
GAGAGCG24150.050.0780947
GATCCGG3700.046.160341
AGCGGCG11850.040.82374610-11
TCGGAGA6150.039.3330154
CGTCGGG6150.039.33019312-13
CGCCGGA9350.038.86660458-59
GTTCGGA2200.038.811632
GGCGCCG15600.038.3652856-57
GCGTCGG6100.038.09756510-11
TTCGGAG4000.037.944793
AAGCGTC3050.037.3200659
TCGGGAA7400.036.534683
AAAGCGG6500.036.4822358
Produced by FastQC (version 0.11.2)